BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0599.0074.p (322 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value STM0862 [O] COG0625 Glutathione S-transferase 192 7e-49 TVN0899 [M] COG1209 dTDP-glucose pyrophosphorylase 180 3e-45 DRA0031 [M] COG1209 dTDP-glucose pyrophosphorylase 147 2e-35 SSO1782 [M] COG1209 dTDP-glucose pyrophosphorylase 143 3e-34 SSO0831 [M] COG1209 dTDP-glucose pyrophosphorylase 142 6e-34 >STM0862 [O] COG0625 Glutathione S-transferase Length = 301 Score = 192 bits (488), Expect = 7e-49, Method: Compositional matrix adjust. Identities = 0/330 (0%), Positives = 0/330 (0%), Gaps = 22/330 (6%) Query: 1 MLPVNGEYILVSIINKLVAAGITEIGIVVN-HTQGEIRRTIGDGERWNASLTFLMQEEPM 59 + + + S + I I + E+ + Sbjct: 26 NKLLGQKISITSRKAQTTRHRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSS 85 Query: 60 GLADAVKSAKSFMADSPFVLMLGDNLYEGDIAPLIRSLETDNAAASILLQQVKDPGQFGV 119 + + N AP+I ++ + Sbjct: 86 IGDVELVIFVVEGTRWTPDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQ 145 Query: 120 AAVHAGQIIGLEEKPKYPKSDLAIVGVYALTAAIWPAMDRLTPSPRGEYEMTDAIQLLIT 179 I E + +P S R +L+ Sbjct: 146 MNFLDIVPISAETGLNVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRF 205 Query: 180 EGGHVVYSIAAEPFFDIGTHDRWLEANRYKMGQDPQNFVKPSGMHSSVQWIPPVRIDPTA 239 G + YS+ E + + N + + G + + I+ Sbjct: 206 LGAELPYSVTVEIERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKI--KTIGIEARK 263 Query: 240 TVMNSV-----------------VGPYVYIGPNSVVEDCILSNSILTDQVTLSHVHA--E 280 + Sbjct: 264 DMQEMFEAPVHLELWVKVKSGWA 323 Query: 281 GSVFGSYVHLAEQEIQEQPARHILGDRASV 310 Sbjct: 324 353 >TVN0899 [M] COG1209 dTDP-glucose pyrophosphorylase Length = 349 Score = 180 bits (457), Expect = 3e-45, Method: Compositional matrix adjust. Identities = 103/282 (36%), Positives = 158/282 (56%), Gaps = 12/282 (4%) Query: 1 MLPVNGEYILVSIINKLVAAGITEIGIVVNHTQG-EIRRTIGDGERWNASLTFLMQEEPM 59 +LP+ G+ I + L+ GI I IV+ G E+++ GDG RWN ++++ Q EP+ Sbjct: 25 LLPIAGKPISEYALENLIEIGIKNINIVIGSVGGLEVKKFYGDGSRWNVNISYTYQPEPL 84 Query: 60 GLADAVKSAKSFMADSPFVLMLGDNLYEGDIAPLIRSLETDNAAASILLQQVKDPGQFGV 119 G+A A+ K+F+ + FV+ LGDN + I+ L + + L V +P QFG+ Sbjct: 85 GIAHAIGLTKAFVGNDDFVVFLGDNYLQNGISNLYEDFTNAGSDGHLGLVPVDNPSQFGI 144 Query: 120 AAVHAGQIIGLEEKPKYPKSDLAIVGVYALTAAIWPAMDRLTPSPRGEYEMTDAIQLLIT 179 A V G+I L EKPK P S+LAIVGVY LT ++ ++DRL PS RGEYE+T+A Q +I Sbjct: 145 AEVDNGKISKLVEKPKTPTSNLAIVGVYFLTPKVFESIDRLKPSKRGEYEITEAYQDMID 204 Query: 180 EGGHVVYSIAAEPFFDIGTHDRWLEANRY---KMGQDPQ--NFVKPSGMHSSVQW----- 229 G + YSI + F D GT D +L NR K+G + + N +H +V+ Sbjct: 205 RGLKISYSIISGWFKDTGTVDDFLACNRLILDKLGDNGRRDNVSGRFDIHPTVKISSDSE 264 Query: 230 -IPPVRIDPTATVMNSVVGPYVYIGPNSVVEDCILSNSILTD 270 + P I + +S +GPY IG N ++++ + +SI+ D Sbjct: 265 VVGPCFIGEGTRIEHSYIGPYTSIGSNCIIKNAEIEDSIIMD 306 >DRA0031 [M] COG1209 dTDP-glucose pyrophosphorylase Length = 361 Score = 147 bits (371), Expect = 2e-35, Method: Compositional matrix adjust. Identities = 103/295 (34%), Positives = 151/295 (51%), Gaps = 17/295 (5%) Query: 1 MLPVNGEYILVSIINKLVAAGITEIGIVVNH-TQGEIRRTIGDGERWNASLTFLMQEEPM 59 +L V G+ I+ I L AGIT+IGIVV+ T+ EI+ + E + LT + Q + + Sbjct: 34 VLRVAGQPIIRHAIRTLTEAGITDIGIVVSDVTRDEIQHAVR--EIRDVKLTLINQHDQL 91 Query: 60 GLADAVKSAKSFMADSPFVLMLGDNLYEGDIAPLIRSLETDNAAASILLQQVKDPGQFGV 119 GL AV +A+ ++ F + LGDNL+E P I S + AA I L +V DP FGV Sbjct: 92 GLGHAVLTAREWVGQDDFCVYLGDNLFEFGARPFIESFHQKHPAALIALVKVADPTAFGV 151 Query: 120 AAVHAGQIIGLEEKPKYPKSDLAIVGVYALTAAIWPAMDRLTPSPRGEYEMTDAIQLLIT 179 A + QI L EKPK P S+LA+ G+Y T I+ +D + PS RGEYE+TD IQ LI Sbjct: 152 AELDGEQITRLVEKPKDPPSNLAVAGLYCFTPQIFEVLDVMPPSARGEYEITDGIQGLIE 211 Query: 180 EGGHVVYSIAAEPFFDIGTHDRWLEANRYKMGQ---DPQNFVKPSGMHSSV--------- 227 G VV + D G L+ANR + Q D Q ++ S M V Sbjct: 212 RGQTVVGQRVEGWWKDTGRPADLLDANRLLLEQIETDVQGDMEGSRMTGRVIVPASSKVL 271 Query: 228 --QWIPPVRIDPTATVMNSVVGPYVYIGPNSVVEDCILSNSILTDQVTLSHVHAE 280 + + PV I + ++ +GP+ IG +V+ + + +S + V + +V Sbjct: 272 RSKIVGPVIIGEDVIIEDAYIGPFTSIGRGTVIRNAEIEHSSVDSGVVIENVQTR 326 >SSO1782 [M] COG1209 dTDP-glucose pyrophosphorylase Length = 344 Score = 143 bits (361), Expect = 3e-34, Method: Compositional matrix adjust. Identities = 101/326 (30%), Positives = 171/326 (52%), Gaps = 24/326 (7%) Query: 1 MLPVNGEYILVSIINKLVAAGITEIGIVV-NHTQGEIRRTIGDGERWNASLTFLMQEEPM 59 ++ V G+ I ++ ++ +GI +I +++ ++T + GDG ++ ++T++ Q + Sbjct: 25 LIKVAGKPISQWVLEQIRDSGIKDILLILGDNTPTRVVDYYGDGSKFGVNITYVYQGKAR 84 Query: 60 GLADAVKSAKSFMADSPFVLMLGDNLYEGDIAPLIRSLETDNAAASILLQQVKDPGQFGV 119 GLADAV K +++ F++ LGDNL D+ R + +ASILL +V +P +FGV Sbjct: 85 GLADAVYRVKDLVSEK-FLVYLGDNLVFYDL----RKFLSFKGSASILLAKVDNPNRFGV 139 Query: 120 AAVHAGQIIGLEEKPKYPKSDLAIVGVYALTAAIWPAMDRLTPSPRGEYEMTDAIQLLIT 179 A ++ ++I L EKPK SDLA+VGVY T I+ +++L PS RGE E+TDAIQ LI Sbjct: 140 AVINENKVIRLVEKPKEKISDLALVGVYGFTDEIFEVIEKLKPSWRGELEITDAIQGLIN 199 Query: 180 EGGHVVYSIAAEPFFDIGTHDRWLEANRYKMGQDPQNFVKPSGMHSSVQW---------- 229 EG V Y I + D GT LEAN + + + + ++ +S++ Sbjct: 200 EGKEVNYEIIQGWWKDTGTPKDILEANSFLLDRYAERKIEGEVENSTIDGRVILEKGAVV 259 Query: 230 -----IPPVRIDPTATVMNSVVGPYVYIGPNSVVEDCILSNSILTDQVTLSHVHAEGSVF 284 P I ++NS +GP+ IG S + + S++ D V + V S+ Sbjct: 260 KNSVIRGPAYIGKDTKIINSYIGPFTSIGDLSEISGSEIEYSVILDNVRIKGVSLMDSLI 319 Query: 285 GSYVHLAEQEIQEQPARHILGDRASV 310 G+ + E + + I+G+ +SV Sbjct: 320 GNN---STVEKGTKWQKLIIGENSSV 342 >SSO0831 [M] COG1209 dTDP-glucose pyrophosphorylase Length = 344 Score = 142 bits (359), Expect = 6e-34, Method: Compositional matrix adjust. Identities = 97/302 (32%), Positives = 158/302 (52%), Gaps = 21/302 (6%) Query: 1 MLPVNGEYILVSIINKLVAAGITEIGIVV-NHTQGEIRRTIGDGERWNASLTFLMQEEPM 59 ++ V G+ + ++ ++ AGI ++ IV+ ++ + GDG ++ +T++ Q + Sbjct: 25 LIKVAGKPVSQWVLEQIRDAGIRDVIIVLGDNAPTRVVEYYGDGSKFGVRITYVYQGKAR 84 Query: 60 GLADAVKSAKSFMADSPFVLMLGDNLYEGDIAPLIRSLETDNAAASILLQQVKDPGQFGV 119 GLADAV K ++D F++ LGDNL D++ + +ASILL +V +P +FGV Sbjct: 85 GLADAVYKVKDVVSDK-FLVYLGDNLVPYDLS----KFSSFKGSASILLAKVNNPNRFGV 139 Query: 120 AAVHAGQIIGLEEKPKYPKSDLAIVGVYALTAAIWPAMDRLTPSPRGEYEMTDAIQLLIT 179 A + G+++ L EKPK SDLA+VGVY T I+ ++ L PS RGE E+TDAIQ LI Sbjct: 140 AVIKDGRVVKLIEKPKEQISDLALVGVYGFTREIFDVIESLKPSWRGELEITDAIQGLID 199 Query: 180 EGGHVVYSIAAEPFFDIGTHDRWLEANRYKMGQDPQNFVKPSGMHSSVQWI--------- 230 G V Y I + D GT LEAN + + + ++ ++ +S+V Sbjct: 200 RGREVNYEIIQGWWKDTGTPKDILEANAFLLDRYAESRIEGVVDNSTVDGKVIIEKGAVI 259 Query: 231 ------PPVRIDPTATVMNSVVGPYVYIGPNSVVEDCILSNSILTDQVTLSHVHAEGSVF 284 P I ++NS +GP+ IG S + D + S++ D V + V S+ Sbjct: 260 KNSVVRGPTYIGRDTKIINSYIGPFTSIGDQSEITDSEIEYSVILDGVKIRGVTLMDSLI 319 Query: 285 GS 286 G+ Sbjct: 320 GN 321 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.134 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 24,231,488 Number of extensions: 1017981 Number of successful extensions: 2178 Number of sequences better than 1.0e-05: 92 Number of HSP's gapped: 2074 Number of HSP's successfully gapped: 92 Length of query: 322 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 216 Effective length of database: 26,153,027 Effective search space: 5649053832 Effective search space used: 5649053832 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 116 (49.3 bits)