BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0599.0052.p (503 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_ydgF [E] COG1113 Gamma-aminobutyrate permease and related per... 460 e-129 BS_aapA [E] COG1113 Gamma-aminobutyrate permease and related per... 428 e-119 CAC1472 [E] COG1113 Gamma-aminobutyrate permease and related per... 410 e-114 ZcycA [E] COG1113 Gamma-aminobutyrate permease and related perme... 392 e-109 cycA [E] COG1113 Gamma-aminobutyrate permease and related permeases 392 e-109 >BS_ydgF [E] COG1113 Gamma-aminobutyrate permease and related permeases Length = 121 Score = 460 bits (1184), Expect = e-129, Method: Compositional matrix adjust. Identities = 0/450 (0%), Positives = 0/450 (0%), Gaps = 1/450 (0%) Query: 43 EVAPQELKRDLENRHVQXXXXXXXXXXXXXXXXXKAIQLAGPSIIFAYLIVGIAVFFVMR 102 +A + + ++ L V A++ + Sbjct: 9 GLAIATEITGTLSMKWASVSEGNGGFILMLVMISLSYIFLSFAVKKIALGVAYALWEGIG 68 Query: 103 ALGELLLSKAGYQSFTDIAEDYLGPRAAFVTGWTYWFCWIMTAMADIIAVGVYVQYWF-D 161 Sbjct: 69 128 Query: 162 IPQWVPAVXXXXXXXXXXXXTVKNFGELEFWFALIKXXXXXXXXXXXXXXXXXXFKTDAG 221 Sbjct: 129 188 Query: 222 TVALSNIWTHGGIFPNGISGFLLSFQMVVFAFVGVELVGVSAAETANPEKSIPSAINKIP 281 Sbjct: 189 248 Query: 282 LRILFFYVGAIIALLSINAWTELTPAESPFVKTFSLVGIPIAAGIINFVVLTSAASACNS 341 Sbjct: 249 308 Query: 342 GMFSTSRILYNLSRNEQAPAHFAKLNKNHVPSNALLVSTLVLSVGALLSKLIPETAFGIV 401 Sbjct: 309 368 Query: 402 TTISAICFIWVWGVILICHLRYKKTKPELQARSKFKAPLTPFINYVVXXXXXXXXXXXXX 461 Sbjct: 369 428 Query: 462 XEETRPAXXXXXXXXXXXXXXYSTNRRKRE 491 Sbjct: 429 458 >BS_aapA [E] COG1113 Gamma-aminobutyrate permease and related permeases Length = 459 Score = 428 bits (1100), Expect = e-119, Method: Compositional matrix adjust. Identities = 217/445 (48%), Positives = 282/445 (63%), Gaps = 1/445 (0%) Query: 47 QELKRDLENRHVQXXXXXXXXXXXXXXXXXKAIQLAGPSIIFAYLIVGIAVFFVMRALGE 106 Q+L R L+NRH+Q K+I AGPSI+FAYLI G+ FF++R+LGE Sbjct: 14 QKLSRGLKNRHIQLMAIGGAIGTGLFLGSGKSIHFAGPSILFAYLITGVFCFFIIRSLGE 73 Query: 107 LLLSKAGYQSFTDIAEDYLGPRAAFVTGWTYWFCWIMTAMADIIAVGVYVQYWF-DIPQW 165 LLLS AGY SF D DYLG AAF+TGWTYWFCWI AMAD+ AVG+Y QYW D+PQW Sbjct: 74 LLLSNAGYHSFVDFVRDYLGNMAAFITGWTYWFCWISLAMADLTAVGIYTQYWLPDVPQW 133 Query: 166 VPAVXXXXXXXXXXXXTVKNFGELEFWFALIKXXXXXXXXXXXXXXXXXXFKTDAGTVAL 225 +P + TVK FGELEFWFALIK F +G +L Sbjct: 134 LPGLLALIILLIMNLATVKLFGELEFWFALIKVIAILALIVTGILLIAKGFSAASGPASL 193 Query: 226 SNIWTHGGIFPNGISGFLLSFQMVVFAFVGVELVGVSAAETANPEKSIPSAINKIPLRIL 285 +N+W+HGG+FPNG GF+LSFQMVVFAFVG+ELVG++A ET NP+K IP AIN+IP+RIL Sbjct: 194 NNLWSHGGMFPNGWHGFILSFQMVVFAFVGIELVGLTAGETENPQKVIPKAINQIPVRIL 253 Query: 286 FFYVGAIIALLSINAWTELTPAESPFVKTFSLVGIPIAAGIINFVVLTSAASACNSGMFS 345 FYVGA+ ++ I W L P ESPFV+ FS VGI +AA +INFVVLTSAASA NS +FS Sbjct: 254 LFYVGALFVIMCIYPWNVLNPNESPFVQVFSAVGIVVAASLINFVVLTSAASAANSALFS 313 Query: 346 TSRILYNLSRNEQAPAHFAKLNKNHVPSNALLVSTLVLSVGALLSKLIPETAFGIVTTIS 405 TSR++Y+L+++ AP KL ++VPSNAL S++ + +G L+ L+PE F ++T++S Sbjct: 314 TSRMVYSLAKDHHAPGLLKKLTSSNVPSNALFFSSIAILIGVSLNYLMPEQVFTLITSVS 373 Query: 406 AICFIWVWGVILICHLRYKKTKPELQARSKFKAPLTPFINYVVXXXXXXXXXXXXXXEET 465 ICFI++WG+ +ICHL+Y+KT+ +KFK P P NY+ +T Sbjct: 374 TICFIFIWGITVICHLKYRKTRQHEAKANKFKMPFYPLSNYLTLAFLAFILVILALANDT 433 Query: 466 RPAXXXXXXXXXXXXXXYSTNRRKR 490 R A Y ++R Sbjct: 434 RIALFVTPVWFVLLIILYKVQTQQR 458 >CAC1472 [E] COG1113 Gamma-aminobutyrate permease and related permeases Length = 455 Score = 410 bits (1054), Expect = e-114, Method: Compositional matrix adjust. Identities = 206/447 (46%), Positives = 277/447 (61%), Gaps = 1/447 (0%) Query: 47 QELKRDLENRHVQXXXXXXXXXXXXXXXXXKAIQLAGPSIIFAYLIVGIAVFFVMRALGE 106 Q L R L+NRHVQ ++I LAGPSI+FAY+I G F VMRALGE Sbjct: 9 QNLSRGLKNRHVQLLAIGGAIGTGLFLGSGRSIHLAGPSILFAYMITGAICFLVMRALGE 68 Query: 107 LLLSKAGYQSFTDIAEDYLGPRAAFVTGWTYWFCWIMTAMADIIAVGVYVQYWF-DIPQW 165 LL+S Y SF D +DYLG AAF+TGWTYWFCWI AMAD+ A G+YVQYW +P+W Sbjct: 69 LLVSNLNYHSFVDFVQDYLGNGAAFITGWTYWFCWISLAMADVTATGLYVQYWIPSLPRW 128 Query: 166 VPAVXXXXXXXXXXXXTVKNFGELEFWFALIKXXXXXXXXXXXXXXXXXXFKTDAGTVAL 225 VP++ V+ FGE+EFWFALIK F T AG + Sbjct: 129 VPSLVILLILLIMNLTAVRLFGEMEFWFALIKVVAILALIIVGIFMIIKGFSTSAGAASF 188 Query: 226 SNIWTHGGIFPNGISGFLLSFQMVVFAFVGVELVGVSAAETANPEKSIPSAINKIPLRIL 285 +N+W HGG FPNG +GF+LSFQMVVFAF G+ELVG++A ET +PE IP AIN IP+RI+ Sbjct: 189 TNLWKHGGWFPNGSNGFILSFQMVVFAFTGIELVGLTAGETEDPEHVIPKAINNIPVRII 248 Query: 286 FFYVGAIIALLSINAWTELTPAESPFVKTFSLVGIPIAAGIINFVVLTSAASACNSGMFS 345 FY+GA+ ++SI W + P +SPFV+ F+ VG+ AA I+NFVVLTSAASACNSG+FS Sbjct: 249 IFYIGALAVIMSIYPWNSINPDKSPFVQVFAAVGVAAAATIVNFVVLTSAASACNSGIFS 308 Query: 346 TSRILYNLSRNEQAPAHFAKLNKNHVPSNALLVSTLVLSVGALLSKLIPETAFGIVTTIS 405 TSR++++L+ AP KL + +PSNA + S V+ + +L+ ++PE F ++T+IS Sbjct: 309 TSRMVFSLANENNAPESMKKLTSHKIPSNATIFSAAVILIAVILNYIMPEGVFVLITSIS 368 Query: 406 AICFIWVWGVILICHLRYKKTKPELQARSKFKAPLTPFINYVVXXXXXXXXXXXXXXEET 465 CFI++W +I+ICH++Y+K PEL +++ FK PL P NY++ ET Sbjct: 369 TFCFIFIWAIIVICHIKYRKANPELASKNNFKMPLYPIANYIILAFFAFVIVTLALNSET 428 Query: 466 RPAXXXXXXXXXXXXXXYSTNRRKREK 492 R A Y ++ K++ Sbjct: 429 RVALFVTPVWFIVLGIIYFISKSKKKN 455 >ZcycA [E] COG1113 Gamma-aminobutyrate permease and related permeases Length = 470 Score = 392 bits (1007), Expect = e-109, Method: Compositional matrix adjust. Identities = 197/428 (46%), Positives = 268/428 (62%), Gaps = 4/428 (0%) Query: 45 APQELKRDLENRHVQXXXXXXXXXXXXXXXXXKAIQLAGPSIIFAYLIVGIAVFFVMRAL 104 A Q L+R+L NRH+Q K I LAGPSIIF Y+I+G +FFVMRA+ Sbjct: 15 AEQSLRRNLTNRHIQLIAIGGAIGTGLFMGSGKTISLAGPSIIFVYMIIGFMLFFVMRAM 74 Query: 105 GELLLSKAGYQSFTDIAEDYLGPRAAFVTGWTYWFCWIMTAMADIIAVGVYVQYWF-DIP 163 GELLLS Y+SF+D A D LGP A + TGWTYWFCW++T MAD++A+ Y Q+WF D+ Sbjct: 75 GELLLSNLEYKSFSDFASDLLGPWAGYFTGWTYWFCWVVTGMADVVAITAYAQFWFPDLS 134 Query: 164 QWVPAVXXXXXXXXXXXXTVKNFGELEFWFALIKXXXXXXXXXXXXXXXXXXFKTDAGTV 223 WV ++ TVK FGE+EFWFA+IK F++ G Sbjct: 135 DWVASLAVIVLLLTLNLATVKMFGEMEFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVE 194 Query: 224 A-LSNIWTHGGIFPNGISGFLLSFQMVVFAFVGVELVGVSAAETANPEKSIPSAINKIPL 282 A +++W GG FP G+SGF FQ+ VFAFVG+ELVG +AAET +PEKS+P AIN IP+ Sbjct: 195 ASFAHLWNDGGWFPKGLSGFFAGFQIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPI 254 Query: 283 RILFFYVGAIIALLSINAWTELTPAESPFVKTFSLVGIPIAAGIINFVVLTSAASACNSG 342 RI+ FYV A+I ++S+ W+ + P +SPFV+ F LVG+P AA +INFVVLTSAAS+ NSG Sbjct: 255 RIIMFYVFALIVIMSVTPWSSVVPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSG 314 Query: 343 MFSTSRILYNLSRNEQAPAHFAKLNKNHVPSNALLVSTLVLSVGALLSKLIPET--AFGI 400 +FSTSR+L+ L++ AP FAKL+K VP+ L S + L G ++ + P AF + Sbjct: 315 VFSTSRMLFGLAQEGVAPKAFAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTM 374 Query: 401 VTTISAICFIWVWGVILICHLRYKKTKPELQARSKFKAPLTPFINYVVXXXXXXXXXXXX 460 +TT+SAI F++VW +IL +L Y+K +P L +S +K PL + +V Sbjct: 375 ITTVSAILFMFVWTIILCSYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLT 434 Query: 461 XXEETRPA 468 ++TR A Sbjct: 435 LEDDTRQA 442 >cycA [E] COG1113 Gamma-aminobutyrate permease and related permeases Length = 470 Score = 392 bits (1007), Expect = e-109, Method: Compositional matrix adjust. Identities = 197/428 (46%), Positives = 268/428 (62%), Gaps = 4/428 (0%) Query: 45 APQELKRDLENRHVQXXXXXXXXXXXXXXXXXKAIQLAGPSIIFAYLIVGIAVFFVMRAL 104 A Q L+R+L NRH+Q K I LAGPSIIF Y+I+G +FFVMRA+ Sbjct: 15 AEQSLRRNLTNRHIQLIAIGGAIGTGLFMGSGKTISLAGPSIIFVYMIIGFMLFFVMRAM 74 Query: 105 GELLLSKAGYQSFTDIAEDYLGPRAAFVTGWTYWFCWIMTAMADIIAVGVYVQYWF-DIP 163 GELLLS Y+SF+D A D LGP A + TGWTYWFCW++T MAD++A+ Y Q+WF D+ Sbjct: 75 GELLLSNLEYKSFSDFASDLLGPWAGYFTGWTYWFCWVVTGMADVVAITAYAQFWFPDLS 134 Query: 164 QWVPAVXXXXXXXXXXXXTVKNFGELEFWFALIKXXXXXXXXXXXXXXXXXXFKTDAGTV 223 WV ++ TVK FGE+EFWFA+IK F++ G Sbjct: 135 DWVASLAVIVLLLTLNLATVKMFGEMEFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVE 194 Query: 224 A-LSNIWTHGGIFPNGISGFLLSFQMVVFAFVGVELVGVSAAETANPEKSIPSAINKIPL 282 A +++W GG FP G+SGF FQ+ VFAFVG+ELVG +AAET +PEKS+P AIN IP+ Sbjct: 195 ASFAHLWNDGGWFPKGLSGFFAGFQIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPI 254 Query: 283 RILFFYVGAIIALLSINAWTELTPAESPFVKTFSLVGIPIAAGIINFVVLTSAASACNSG 342 RI+ FYV A+I ++S+ W+ + P +SPFV+ F LVG+P AA +INFVVLTSAAS+ NSG Sbjct: 255 RIIMFYVFALIVIMSVTPWSSVVPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSG 314 Query: 343 MFSTSRILYNLSRNEQAPAHFAKLNKNHVPSNALLVSTLVLSVGALLSKLIPET--AFGI 400 +FSTSR+L+ L++ AP FAKL+K VP+ L S + L G ++ + P AF + Sbjct: 315 VFSTSRMLFGLAQEGVAPKAFAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTM 374 Query: 401 VTTISAICFIWVWGVILICHLRYKKTKPELQARSKFKAPLTPFINYVVXXXXXXXXXXXX 460 +TT+SAI F++VW +IL +L Y+K +P L +S +K PL + +V Sbjct: 375 ITTVSAILFMFVWTIILCSYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLT 434 Query: 461 XXEETRPA 468 ++TR A Sbjct: 435 LEDDTRQA 442 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.325 0.139 0.427 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 30,156,698 Number of extensions: 1043912 Number of successful extensions: 4169 Number of sequences better than 1.0e-05: 141 Number of HSP's gapped: 3892 Number of HSP's successfully gapped: 144 Length of query: 503 Length of database: 38,378,431 Length adjustment: 111 Effective length of query: 392 Effective length of database: 25,576,357 Effective search space: 10025931944 Effective search space used: 10025931944 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 118 (50.1 bits)