BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0599.0025.p
         (389 letters)

Database: COG 
           115,334 sequences; 38,378,431 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

BS_aprE [O] COG1404 Subtilisin-like serine proteases                  275   9e-74
BH0684 [O] COG1404 Subtilisin-like serine proteases                   248   1e-65
BH0855 [O] COG1404 Subtilisin-like serine proteases                   239   4e-63
BS_epr [O] COG1404 Subtilisin-like serine proteases                   224   2e-58
BH1491 [O] COG1404 Subtilisin-like serine proteases                   195   1e-49

>BS_aprE [O] COG1404 Subtilisin-like serine proteases
          Length = 381

 Score =  275 bits (703), Expect = 9e-74,   Method: Compositional matrix adjust.
 Identities = 165/383 (43%), Positives = 228/383 (59%), Gaps = 15/383 (3%)

Query: 4   IRLLSSFCLITVMLTSAGALAASESSDPSIAKTYDGTYLVKLNSVTTPPN-DVIEKLGGK 62
           I LL +  LI  M  S  ++ A+  S  S  K Y   +   ++++++    DVI + GGK
Sbjct: 8   ISLLFALTLIFTMAFSNMSVQAAGKS--STEKKYIVGFKQTMSAMSSAKKKDVISEKGGK 65

Query: 63  LKKISESGDIVMAELSGSSLYELKNSPDVQYIEPNYEVKTEVEAEKIESVTETIPYGIQN 122
           ++K  +  +   A L   ++ ELK  P V Y+E         E        +++PYGI  
Sbjct: 66  VQKQFKYVNAAAATLDEKAVKELKKDPSVAYVE---------EDHIAHEYAQSVPYGISQ 116

Query: 123 IHAIEAQQEGYTGKGVKIAVLDSGY-SQHSDVKVKSGYSFITNSEN-YDDDFGHGTHVAG 180
           I A     +GYTG  VK+AV+DSG  S H D+ V+ G SF+ +  N Y D   HGTHVAG
Sbjct: 117 IKAPALHSQGYTGSNVKVAVIDSGIDSSHPDLNVRGGASFVPSETNPYQDGSSHGTHVAG 176

Query: 181 IIAASMNGEGVVGVAPMADIYAIKVLDQGGRGSYFTLIEGIDWAIKHKMDIINISFGGEN 240
            IAA  N  GV+GV+P A +YA+KVLD  G G Y  +I GI+WAI + MD+IN+S GG  
Sbjct: 177 TIAALNNSIGVLGVSPSASLYAVKVLDSTGSGQYSWIINGIEWAISNNMDVINMSLGGPT 236

Query: 241 PSQILEEKIQEAYDAGILIIAAAGNDGYMGD-DTILYPARYSTAIAVGAIDSSNKRAFFS 299
            S  L+  + +A  +GI++ AAAGN+G  G   T+ YPA+Y + IAVGA++SSN+RA FS
Sbjct: 237 GSTALKTVVDKAVSSGIVVAAAAGNEGSSGSTSTVGYPAKYPSTIAVGAVNSSNQRASFS 296

Query: 300 AKGPDLDLVAPGVKVLSTLPSNQYGMKSGTSMAAPHVTGVAALILDKNKKLSPGEVADIL 359
           + G +LD++APGV + STLP   YG  +GTSMA PHV G AALIL K+   +  +V D L
Sbjct: 297 SAGSELDVMAPGVSIQSTLPGGTYGAYNGTSMATPHVAGAAALILSKHPTWTNAQVRDRL 356

Query: 360 ESTAEPLGVVPEYGHGIVQAKEA 382
           ESTA  LG    YG G++  + A
Sbjct: 357 ESTATYLGNSFYYGKGLINVQAA 379


>BH0684 [O] COG1404 Subtilisin-like serine proteases
          Length = 372

 Score =  248 bits (634), Expect = 1e-65,   Method: Compositional matrix adjust.
 Identities = 139/317 (43%), Positives = 192/317 (60%), Gaps = 17/317 (5%)

Query: 72  IVMAELSGSSLYELKNSPDVQYIEPNYEVKTEVEAEKIESVTETIPYGIQNIHAIE---A 128
           ++++ELS      L+N P V++IE N  V  +             P  + NI   +    
Sbjct: 69  VIVSELSTEVAQLLENDPSVEFIEKNERVYLD-------------PLVMNNIQETDIPKL 115

Query: 129 QQEGYTGKGVKIAVLDSGYSQHSDVKVKSGYSFITNSENYDDDFGHGTHVAGIIAASMNG 188
           ++    G GVKIAVLD+G + H D+ V  G SF++    Y D  GHGTHVAG IAA  N 
Sbjct: 116 EERMKRGDGVKIAVLDTGIASHDDLHVIDGVSFVSVEPFYRDLNGHGTHVAGTIAAQEND 175

Query: 189 EGVVGVAPMADIYAIKVLDQGGRGSYFTLIEGIDWAIKHKMDIINISFGGENPSQILEEK 248
           E   G+AP  ++YA+KVL+  G GS  ++  G+DWAI + MDIIN+S G    S+ L+  
Sbjct: 176 EASTGIAPNVELYAVKVLNGLGAGSIASITNGVDWAISNDMDIINMSLGTNTDSEALQRA 235

Query: 249 IQEAYDAGILIIAAAGNDGYMG-DDTILYPARYSTAIAVGAIDSSNKRAFFSAKGPDLDL 307
           ++ A+D GILIIAAAGN G      TI YPARY + +AVGA+D +N+RA FS+ G  L++
Sbjct: 236 VERAHDHGILIIAAAGNSGEADKQHTIDYPARYDSVVAVGAVDGNNERASFSSYGEQLEI 295

Query: 308 VAPGVKVLSTLPSNQYGMKSGTSMAAPHVTGVAALILDKNKKLSPGEVADILESTAEPLG 367
           +APGV++ ST   N+Y   SGTSMA+PHVTG AALI   N +L+  ++   L +TA PLG
Sbjct: 296 MAPGVEIHSTFLFNRYERLSGTSMASPHVTGAAALIKSNNPELTNEQIRKRLNTTATPLG 355

Query: 368 VVPEYGHGIVQAKEAVD 384
               YGHG++    A+D
Sbjct: 356 NPFYYGHGLLNVDAALD 372


>BH0855 [O] COG1404 Subtilisin-like serine proteases
          Length = 361

 Score =  239 bits (611), Expect = 4e-63,   Method: Compositional matrix adjust.
 Identities = 132/294 (44%), Positives = 186/294 (63%), Gaps = 13/294 (4%)

Query: 89  PDVQYIEPNYEVKTEVEAEKIESVTETIPYGIQNIHAIEAQQEGYTGKGVKIAVLDSGYS 148
           P+V+ IE N EV          ++++T+P+GI  I+  +A   G  G G ++AVLD+G +
Sbjct: 79  PNVKAIEKNAEV----------TISQTVPWGISFINTQQAHNRGIFGNGARVAVLDTGIA 128

Query: 149 QHSDVKVKSGYSFITNSENYDDDFGHGTHVAGIIAASMNGEGVVGVAPMADIYAIKVLDQ 208
            H D+++  G SFI++  +Y D+ GHGTHVAG IAA  N  GV+GVAP AD+YA+KVLD+
Sbjct: 129 SHPDLRIAGGASFISSEPSYHDNNGHGTHVAGTIAALNNSIGVLGVAPSADLYAVKVLDR 188

Query: 209 GGRGSYFTLIEGIDWAIKHKMDIINISFGGENPSQILEEKIQEAYDAGILIIAAAGNDGY 268
            G GS  ++ +GI+WAI + M IIN+S G  + S  LE  +  A +AGIL++ AAGN G 
Sbjct: 189 NGSGSLASVAQGIEWAINNNMHIINMSLGSTSGSSTLELAVNRANNAGILLVGAAGNTGR 248

Query: 269 MGDDTILYPARYSTAIAVGAIDSSNKRAFFSAKGPDLDLVAPGVKVLSTLPSNQYGMKSG 328
            G   + YPARYS  +AV A+D + +RA FS  GP++++ APGV V ST   N+Y   SG
Sbjct: 249 QG---VNYPARYSGVMAVAAVDQNGQRASFSTYGPEIEISAPGVNVNSTYTGNRYVSLSG 305

Query: 329 TSMAAPHVTGVAALILDKNKKLSPGEVADILESTAEPLGVVPEYGHGIVQAKEA 382
           TSMA PHV GVAAL+  +    +  ++   +  TA  LG    YG+G+V A  A
Sbjct: 306 TSMATPHVAGVAALVKSRYPSYTNNQIRQRINQTATYLGSPSLYGNGLVHAGRA 359


>BS_epr [O] COG1404 Subtilisin-like serine proteases
          Length = 645

 Score =  224 bits (571), Expect = 2e-58,   Method: Compositional matrix adjust.
 Identities = 119/317 (37%), Positives = 188/317 (59%), Gaps = 11/317 (3%)

Query: 81  SLYELKNSPDVQYIEPN--YEVKTEVEAEKIESVTETI----PYGIQNIHAIEAQQEGYT 134
           ++ ELK  PD+ Y+E N  +      + + +   T+T      + ++ I   +A + G T
Sbjct: 73  TVKELKQDPDILYVENNVSFTAADSTDFKVLSDGTDTSDNFEQWNLEPIQVKQAWKAGLT 132

Query: 135 GKGVKIAVLDSGYSQHSDVKVKSGYSFITNSENYDDDFGHGTHVAGIIAASMNGEGVVGV 194
           GK +KIAV+DSG S H D+ +  GYS ++ + +Y DD GHGTHVAGII A  NG G+ G+
Sbjct: 133 GKNIKIAVIDSGISPHDDLSIAGGYSAVSYTSSYKDDNGHGTHVAGIIGAKHNGYGIDGI 192

Query: 195 APMADIYAIKVLDQGGRGSYFTLIEGIDWAIKHKMDIINISFGGENPSQILEEKIQEAYD 254
           AP A IYA+K LDQ G G   +L++GIDW+I ++MDI+N+S G  + S+IL + + +AY+
Sbjct: 193 APEAQIYAVKALDQNGSGDLQSLLQGIDWSIANRMDIVNMSLGTTSDSKILHDAVNKAYE 252

Query: 255 AGILIIAAAGNDGYMGDDTILYPARYSTAIAVGAIDSSNKRAFFSAKGPDLDLVAPGVKV 314
            G+L++AA+GNDG      + YPA YS+ +AV A +  N+ A FS  G +++  APG  +
Sbjct: 253 QGVLLVAASGNDG--NGKPVNYPAAYSSVVAVSATNEKNQLASFSTTGDEVEFSAPGTNI 310

Query: 315 LSTLPSNQYGMKSGTSMAAPHVTGVAALILDKNKKLSPGEVADILESTAEPLGVV---PE 371
            ST  +  Y   SGTS A PH   + AL+  ++   +  ++ + +      LG      +
Sbjct: 311 TSTYLNQYYATGSGTSQATPHAAAMFALLKQRDPAETNVQLREEMRKNIVDLGTAGRDQQ 370

Query: 372 YGHGIVQAKEAVDQTAW 388
           +G+G++Q K     +A+
Sbjct: 371 FGYGLIQYKAQATDSAY 387


>BH1491 [O] COG1404 Subtilisin-like serine proteases
          Length = 588

 Score =  195 bits (496), Expect = 1e-49,   Method: Compositional matrix adjust.
 Identities = 120/311 (38%), Positives = 176/311 (56%), Gaps = 19/311 (6%)

Query: 85  LKNSPDVQYIEPNYEVKTEVEAEKIESVTETIPYGIQNIHAIEAQQEGYT---GKGVKIA 141
           L    D+ + EPNY    +++A  +    E       N+  I+ + EG++   G+ VKIA
Sbjct: 279 LSRDYDLDFAEPNYRFTKQIQA-PVTPNDEFFKEYQWNLQQIDIE-EGWSLASGENVKIA 336

Query: 142 VLDSGYS-QHSDVKVK--SGYSFITNSENYDDDFGHGTHVAGIIAASMNG-EGVVGVAPM 197
           +LD+G    H D+K K  +GY+ +  + N+ D  GHGTHVAG+ AA  N   G+ G++  
Sbjct: 337 ILDTGVDPNHPDIKDKIVNGYNAVEGNNNFADKHGHGTHVAGVAAAVTNNVTGIAGISWK 396

Query: 198 ADIYAIKVLDQGGRGSYFTLIEGIDWAIKHKMDIINISFGGENPSQILEEKIQEAYDAGI 257
           ++I  +KVL+  G GS F + +GI WA  H   +IN+S G    S  L + ++ AYD  +
Sbjct: 397 SEILPVKVLNDNGEGSSFEVAKGIYWATDHGAKVINMSLGDYYHSDALRDAVKYAYDHDV 456

Query: 258 LIIAAAGNDGYMGDDTILYPARYSTAIAVGAIDSSNKRAFFSAKGPDLDLVAPGVKVLST 317
           ++IAA+GND     +  LYPA Y   + V A+D +  RAFFS  G  +D+ APG  + S 
Sbjct: 457 VLIAASGNDNV---EDPLYPAIYEEVLTVAAVDDTRNRAFFSNFGKHIDVTAPGEHIPSL 513

Query: 318 LPSNQYGMKSGTSMAAPHVTGVAALILDKNKKLSPGEVADILESTAEPLGVVPE-----Y 372
            P NQY + SGTSMA+PH  G+A LI      LS  EV DI++ TA+ LG  P+     Y
Sbjct: 514 FPDNQYTVMSGTSMASPHAAGLAGLIRSLRPDLSNQEVMDIMKKTAKDLG--PKGHDVYY 571

Query: 373 GHGIVQAKEAV 383
           GHG +  + A+
Sbjct: 572 GHGEIDIEAAL 582


  Database: COG
    Posted date:  Aug 3, 2010  9:58 AM
  Number of letters in database: 38,378,431
  Number of sequences in database:  115,334
  
Lambda     K      H
   0.312    0.132    0.367 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 115334
Number of Hits to DB: 30,044,218
Number of extensions: 1378203
Number of successful extensions: 3733
Number of sequences better than 1.0e-05: 59
Number of HSP's gapped: 3605
Number of HSP's successfully gapped: 63
Length of query: 389
Length of database: 38,378,431
Length adjustment: 108
Effective length of query: 281
Effective length of database: 25,922,359
Effective search space: 7284182879
Effective search space used: 7284182879
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 117 (49.7 bits)