BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0599.0021.p (450 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value lin0989 [V] COG0534 Na+-driven multidrug efflux pump 328 1e-89 L112499 [V] COG0534 Na+-driven multidrug efflux pump 301 1e-81 CAC2485 [V] COG0534 Na+-driven multidrug efflux pump 298 2e-80 NMA1121 [I] COG1607 Acyl-CoA hydrolase 161 2e-39 MA1121 [V] COG0534 Na+-driven multidrug efflux pump 161 2e-39 >lin0989 [V] COG0534 Na+-driven multidrug efflux pump Length = 1091 Score = 328 bits (840), Expect = 1e-89, Method: Compositional matrix adjust. Identities = 29/440 (6%), Positives = 75/440 (17%), Gaps = 2/440 (0%) Query: 1 MDAENLYYFEKAPVAKAVAHFAVPMMLGTSMNVIYSILNAYFLGXXXXXXXXXXXXXXXX 60 M + + + T + + + F Sbjct: 1 MHRYVQKDIQGTTEPQFRNSHSRTSGTVTLYLPVSTFVGTGFFIHPQSKEKLMKRHLNTC 60 Query: 61 XXXIIMALGNLIGMGSGTFISRLLGEKRYDDVKHVSSFAFYSSLVLGLILMAVGLPLIEL 120 + + + S +R + + + ++ + L Sbjct: 61 YRLVWNHMTGAFVVASELARARGKRGGVAVALSLAAVTSLPVLAADIVVHPGETVNGGTL 120 Query: 121 IVHGLGATPDSFGFTKDYVTVMLIGSPFVVLFFTLENLVRSEGAAMTSMIGMILSVVVNI 180 H + + + + G+ Sbjct: 121 ANHDNQIVFGTTNGMTISTGLEYGPDNEANTGGQWVQDGGTANKTTVTSGGLQRVNPGGS 180 Query: 181 ILDALVIFVFHWDVIGVASATVISNLVASIYYAFHMGSKSQFLTISVKWFKATKDILGNV 240 + D ++ + G A T ++ ++ T V Sbjct: 181 VSDTVISAGGGQSLQGRAVNTTLNGGEQWMHEGAIATGTVINDKGWQVVKPGTVATDTVV 240 Query: 241 FKIGVPVFIMSIFLGAMSLIFNHFLVEYGDAAVAAYGISSRLLQFPEFILMGLCEGVVPL 300 + + + L Sbjct: 241 NTGAEGGPDAENGDTGQFVRGDAVRTTINKNGRQIVRAEGTANTTVVYAGGDQTVHGHAL 300 Query: 301 IAFSFTADKL-RMKNTIGFTIKAIVGLAVVFGIVVYLISDHLIGLFTNDPQLIEMGSYIL 359 + T T+ G +V V + + L Sbjct: 301 DTTLNGGYQYVHNGGTASDTVVNSDGWQIVKNGGVAGNTTVNQKGRLQVDAGGTATNVTL 360 Query: 360 HVTFLSLFITGMTTLFTGVFQATAQGTAAFIMSIIQGVTLIPVLYIANQMNGFHGVVWSL 419 + T T A + +++ + VL N +L Sbjct: 361 KQGGALVTSTAATVTGINRLGAFSVVEGKADNVVLENGGRLDVLTGHTATNTRVDDGGTL 420 Query: 420 VIADAAAFLVGAVMLYVLRN 439 + + M Sbjct: 421 DVRNGGTATTV-SMGNGGVL 439 >L112499 [V] COG0534 Na+-driven multidrug efflux pump Length = 459 Score = 301 bits (772), Expect = 1e-81, Method: Compositional matrix adjust. Identities = 169/453 (37%), Positives = 270/453 (59%), Gaps = 9/453 (1%) Query: 2 DAENLYYFEKAPVAKAVAHFAVPMMLGTSMNVIYSILNAYFLGXXXXXXXXXXXXXXXXX 61 N YY ++AP+ KA+AH ++PMM+G S IY+++NAYF+G Sbjct: 3 KKSNSYYLKEAPIRKAIAHLSIPMMIGISATTIYNLINAYFIGLVHDTNMMSTITLGLTI 62 Query: 62 XXIIMALGNLIGMGSGTFISRLLGEKRYDDVKHVSSFAFYSSLVLGLILMAVGLPLIELI 121 + MA+GNL G+G GT+I+RLLG + K ++ ++FY SL++GL++ V L + I Sbjct: 63 TIVFMAIGNLFGVGGGTYITRLLGSGDKEKGKKIAGYSFYMSLIIGLLIGLVSLFFLSPI 122 Query: 122 VHGLGATPDSFGFTKDYVTVMLIGSPFVVLFFTLENLVRSEGAAMTSMIGMILSVVVNII 181 H LG+ + +TK+Y V+L G ++L + LE LVR+EGA+ SM GM + V +I+ Sbjct: 123 AHILGSDAATLTYTKEYAAVLLGGGFSMILNYGLEQLVRAEGASKESMYGMFVGVATSIV 182 Query: 182 LDALVIFVFHWDVIGVASATVISNLVASIYYAFHMGSKSQFLTISVKWFKATKDILGNVF 241 LDAL I V + VIG + VI+NL ++ YY + + +KS+ L +K FK + ++ Sbjct: 183 LDALFILVLNLHVIGAGLSMVIANLASTAYYVWFLENKSESLKGFLKHFKISLKDQFEIY 242 Query: 242 KIGVPVFIMSIFLGAMSLIFNHFLVEYGDAAVAAYGISSRLLQFPEFILMGLCEGVVPLI 301 KIG+ S F+ +L+ NHF V+YGD +A++GI+ R+ Q PEF+ MGL G +PL Sbjct: 243 KIGIAEVFKSAFMIVTTLLLNHFSVQYGDNVLASFGIALRITQLPEFLTMGLFLGAIPLF 302 Query: 302 AFSFTADKL-RMKNTIGFTIKAIVGLAVVFGIVVYLISDHLIGLFTNDPQLIEMGSYILH 360 A+SF ++ R+K + I G++++F ++VYL ++ LFT+D +I++G +L Sbjct: 303 AYSFASNNTQRLKEALKELTLWIGGISILFALIVYLFRVPVMQLFTDDSAVIKVGLIVLA 362 Query: 361 VTFLSLFITGMTTLFTGVFQATAQGTAAFIMSIIQGVTLIPVLYIANQMNGFHGVVWSLV 420 +S G + LFTG+F+A+ G +MS IQG+ IP++ I + G G++WS+ Sbjct: 363 AQLVSSVFNGFSGLFTGIFEASGLGAQTGVMSTIQGILYIPIVIILHHFYGLTGLIWSMT 422 Query: 421 IADAAAFLVGAVML--YVLR---NKL---QPEL 445 + + AF+ G +ML Y+L+ KL QPEL Sbjct: 423 LTEGIAFVAGLIMLIPYLLKLNNGKLMENQPEL 455 >CAC2485 [V] COG0534 Na+-driven multidrug efflux pump Length = 442 Score = 298 bits (762), Expect = 2e-80, Method: Compositional matrix adjust. Identities = 166/437 (37%), Positives = 268/437 (61%), Gaps = 2/437 (0%) Query: 1 MDAENLYYFEKAPVAKAVAHFAVPMMLGTSMNVIYSILNAYFLGXXXXXXXXXXXXXXXX 60 M++ N YY EKAP+ KAV +VPMM+G S+ IY+++NA+F+G Sbjct: 1 MESSNEYYLEKAPIRKAVFSLSVPMMIGMSVGTIYNVINAFFIGLMHNTNMLTAVTLGLP 60 Query: 61 XXXIIMALGNLIGMGSGTFISRLLGEKRYDDVKHVSSFAFYSSLVLGLILMAVGLPLIEL 120 I+MA GN+ G+G G FI+RL+ + + ++ K + + FYSS++ G+I+ + I Sbjct: 61 IFTILMAFGNMFGVGGGNFITRLIAKNKVNEAKSAAGYTFYSSMIAGVIIAVAAVVFINP 120 Query: 121 IVHGLGATPDSFGFTKDYVTVMLIGSPFVVLFFTLENLVRSEGAAMTSMIGMILSVVVNI 180 IV LGA S +TK+Y M +G V+L F LE +VR+EGA+ SM GM +S +N Sbjct: 121 IVKILGADSLSSSYTKNYALTMFLGGFAVILNFALEQMVRAEGASKESMYGMFVSTALNF 180 Query: 181 ILDALVIFVFHWDVIGVASATVISNLVASIYYAFHMGSKSQFLTISVKWFKATKDILGNV 240 I D L I VF +V+G A+A +++NL +SIYY +++ KS+ L + +FK + + Sbjct: 181 IFDPLFILVFKLNVVGAAAAMLLANLGSSIYYIYYLDKKSEKLRGFMGYFKISIKNKMEI 240 Query: 241 FKIGVPVFIMSIFLGAMSLIFNHFLVEYGDAAVAAYGISSRLLQFPEFILMGLCEGVVPL 300 +KIG+ +MS FL +L+ N+F + YGD+ VA +GI+ R++Q PEF+ MG+ GV+PL Sbjct: 241 YKIGISELLMSFFLIITTLLLNNFSIGYGDSVVAGFGIALRIVQVPEFLSMGVMMGVMPL 300 Query: 301 IAFSFTADKL-RMKNTIGFTIKAIVGLAVVFGIVVYLISDHLIGLFTNDPQLIEMGSYIL 359 IA++F+ R+ I ++ IV L+ +FG +VY+ ++ F+ DP ++++G YI+ Sbjct: 301 IAYNFSKKNFARLTEGIKTSVLFIVILSAIFGGIVYVFRVEVLHFFSKDPSVMKIGGYIM 360 Query: 360 HVTFLSLFITGMTTLFTGVFQATAQGTAAFIMSIIQGVTLIPVLYIANQMNGFHGVVWSL 419 +S G T LFTG+FQA+ +G ++ IM++ QG+ IPV+ + + G GV+WS+ Sbjct: 361 LAMLISSMFNGTTGLFTGIFQASGKGISSTIMAVTQGMLYIPVIILLHHFFGLDGVIWSM 420 Query: 420 VIADAAAFLVGAVMLYV 436 I + F V A++LY+ Sbjct: 421 TITEIITFFV-ALILYI 436 >NMA1121 [I] COG1607 Acyl-CoA hydrolase Length = 475 Score = 161 bits (407), Expect = 2e-39, Method: Compositional matrix adjust. Identities = 124/444 (27%), Positives = 222/444 (50%), Gaps = 20/444 (4%) Query: 4 ENLYYFEKAPVAKAVAHFAVPMMLGTSMNVIYSILNAYFLGXXXXXXXXXX---XXXXXX 60 E + K K + + P+++G + IY++++ +F+G Sbjct: 10 EKSEFLGKESTGKLLFKLSTPVVIGMLVQAIYNVVDTFFVGVAYGTESVQAIGGLSIAFP 69 Query: 61 XXXIIMALGNLIGMGSGTFISRLLGEKRYDDVKHVSSFAFYSSLVLGLILMAVGLPLIEL 120 IIMA G ++G G + ISR LG + + F SL+L +++ L +E Sbjct: 70 VQMIIMAFGIVLGTGGSSIISRALGARENKKAERTLGNVFSLSLILSMLIAVPCLFYLEP 129 Query: 121 IVHGLGATPDSFGFTKDYVTVMLIGSPFVVLFFTLENLVRSEGAAMTSMIGMILSVVVNI 180 I+ GATP + +DY+ ++++G F V ++N+VRSEG A +M M++ +NI Sbjct: 130 ILRVFGATPGVLPYARDYLNIIILGGIFFVFGVAVQNIVRSEGNARLAMNAMLIGGGLNI 189 Query: 181 ILDALVIFVFHWDVIGVASATVISNLVASI----YYAFHMGS---KSQFLTISVKWFKAT 233 +LD + +F F V G A ATV++ VAS+ YY MGS +S+ L +K Sbjct: 190 LLDPVFMFGFGMGVKGAAIATVLAQAVASVFLLLYYLKGMGSVHFRSETLKPDLK----- 244 Query: 234 KDILGNVFKIGVPVFIMSIFLGAMSLIFNHFLVEY-GDAAVAAYGISSRLLQFPEFILMG 292 I+ + IG F+M M + + L +Y GD A+A +GI+ ++ F L+G Sbjct: 245 --IIKEIGAIGTGSFVMESASSLMMIFVYNALADYGGDVAIAVFGITMKINSFIFLPLLG 302 Query: 293 LCEGVVPLIAFSFTADKL-RMKNTIGFTIKAIVGLAVVFGIVVYLISDHLIGLFTNDPQL 351 + G+ P++ F++ A K R+ + + A ++ ++VYL ++ L+ +F+ DP+ Sbjct: 303 MAFGLQPIVGFNYGAKKYGRIVEAVKLALVAATTFGLLGLLIVYLFAEKLLIIFSADPEY 362 Query: 352 IEMGSYILHVTFLSLFITGMTTLFTGVFQATAQGTAAFIMSI-IQGVTLIPVLYIANQMN 410 +E+G + FL + G+ + T +FQA + AFI+S+ Q + LIP + + + Sbjct: 363 LELGKIAARIMFLGTPLIGLNVVTTILFQALGKARPAFILSVSRQLLFLIPAVVLLPRFY 422 Query: 411 GFHGVVWSLVIADAAAFLVGAVML 434 +GV + ++D AF++ ML Sbjct: 423 ALNGVWAAFPVSDLLAFMLSGFML 446 >MA1121 [V] COG0534 Na+-driven multidrug efflux pump Length = 475 Score = 161 bits (407), Expect = 2e-39, Method: Compositional matrix adjust. Identities = 124/444 (27%), Positives = 222/444 (50%), Gaps = 20/444 (4%) Query: 4 ENLYYFEKAPVAKAVAHFAVPMMLGTSMNVIYSILNAYFLGXXXXXXXXXX---XXXXXX 60 E + K K + + P+++G + IY++++ +F+G Sbjct: 10 EKSEFLGKESTGKLLFKLSTPVVIGMLVQAIYNVVDTFFVGVAYGTESVQAIGGLSIAFP 69 Query: 61 XXXIIMALGNLIGMGSGTFISRLLGEKRYDDVKHVSSFAFYSSLVLGLILMAVGLPLIEL 120 IIMA G ++G G + ISR LG + + F SL+L +++ L +E Sbjct: 70 VQMIIMAFGIVLGTGGSSIISRALGARENKKAERTLGNVFSLSLILSMLIAVPCLFYLEP 129 Query: 121 IVHGLGATPDSFGFTKDYVTVMLIGSPFVVLFFTLENLVRSEGAAMTSMIGMILSVVVNI 180 I+ GATP + +DY+ ++++G F V ++N+VRSEG A +M M++ +NI Sbjct: 130 ILRVFGATPGVLPYARDYLNIIILGGIFFVFGVAVQNIVRSEGNARLAMNAMLIGGGLNI 189 Query: 181 ILDALVIFVFHWDVIGVASATVISNLVASI----YYAFHMGS---KSQFLTISVKWFKAT 233 +LD + +F F V G A ATV++ VAS+ YY MGS +S+ L +K Sbjct: 190 LLDPVFMFGFGMGVKGAAIATVLAQAVASVFLLLYYLKGMGSVHFRSETLKPDLK----- 244 Query: 234 KDILGNVFKIGVPVFIMSIFLGAMSLIFNHFLVEY-GDAAVAAYGISSRLLQFPEFILMG 292 I+ + IG F+M M + + L +Y GD A+A +GI+ ++ F L+G Sbjct: 245 --IIKEIGAIGTGSFVMESASSLMMIFVYNALADYGGDVAIAVFGITMKINSFIFLPLLG 302 Query: 293 LCEGVVPLIAFSFTADKL-RMKNTIGFTIKAIVGLAVVFGIVVYLISDHLIGLFTNDPQL 351 + G+ P++ F++ A K R+ + + A ++ ++VYL ++ L+ +F+ DP+ Sbjct: 303 MAFGLQPIVGFNYGAKKYGRIVEAVKLALVAATTFGLLGLLIVYLFAEKLLIIFSADPEY 362 Query: 352 IEMGSYILHVTFLSLFITGMTTLFTGVFQATAQGTAAFIMSI-IQGVTLIPVLYIANQMN 410 +E+G + FL + G+ + T +FQA + AFI+S+ Q + LIP + + + Sbjct: 363 LELGKIAARIMFLGTPLIGLNVVTTILFQALGKARPAFILSVSRQLLFLIPAVVLLPRFY 422 Query: 411 GFHGVVWSLVIADAAAFLVGAVML 434 +GV + ++D AF++ ML Sbjct: 423 ALNGVWAAFPVSDLLAFMLSGFML 446 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.330 0.143 0.415 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 29,188,365 Number of extensions: 1192921 Number of successful extensions: 4374 Number of sequences better than 1.0e-05: 82 Number of HSP's gapped: 4220 Number of HSP's successfully gapped: 84 Length of query: 450 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 340 Effective length of database: 25,691,691 Effective search space: 8735174940 Effective search space used: 8735174940 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 117 (49.7 bits)