BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0599.0010.p (249 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SMa0059 [IQR] COG1028 Dehydrogenases with different specificitie... 95 1e-19 CC0094 [IQR] COG1028 Dehydrogenases with different specificities... 93 3e-19 TM0441 [IQR] COG1028 Dehydrogenases with different specificities... 89 9e-18 PAE2832 [IQR] COG1028 Dehydrogenases with different specificitie... 88 1e-17 PAE1589 [IQR] COG1028 Dehydrogenases with different specificitie... 86 4e-17 >SMa0059 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 261 Score = 94.7 bits (234), Expect = 1e-19, Method: Compositional matrix adjust. Identities = 79/254 (31%), Positives = 124/254 (48%), Gaps = 28/254 (11%) Query: 13 FKGKRVVVLGGTSGIGFATAEAAAREGSSLVVVSSQKERADRAVSRLPEGTEGYAVDLTN 72 F GK +VV GG+ G+G A A A G + +V++ K D AV+ + + G+ D+ + Sbjct: 4 FNGKSIVVTGGSLGMGLACAHRFAAGGGKVTIVANDKASVDEAVTSIGDNAAGFVGDVRS 63 Query: 73 EEQ----VRQFFSEIGEFDHLVFTAGDPLMVESLDVINVDTAHRLF----NVRYWGAFMA 124 + V+ S G D L AG ++ + VDTA ++ ++ G F+A Sbjct: 64 KADMNAAVQAAVSRHGGVDILACCAG----IQRYGTV-VDTADEVWDDVLDINLKGIFLA 118 Query: 125 AKYGSRNIRQ--GGSITLTSGVVGVRPQKGFTVAASICGAVESLTRALAVELS--PLRVN 180 +K+ +R+ GG+I S V Q G + GA+ +L RA+A++ + + VN Sbjct: 119 SKFAIPEMRKRGGGAIVAISSVQAYASQTGVAAYTASKGAINALVRAMALDHAGDNITVN 178 Query: 181 AVCFGLMRT-------ELWDGIPEEHRNAMYENAGRSLPVGRVGEPEDGAEAFLYLMREK 233 AVC + T +LW G E A E G+ P+GRVG+P + AE +L EK Sbjct: 179 AVCPASIDTPMLRWAADLWKG--EGTVEATLETWGKGHPLGRVGKPSEVAELVAFLASEK 236 Query: 234 --YSTGQIIVVDGG 245 + TG I +DGG Sbjct: 237 ARFITGADIKIDGG 250 >CC0094 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 296 Score = 93.2 bits (230), Expect = 3e-19, Method: Compositional matrix adjust. Identities = 78/253 (30%), Positives = 116/253 (45%), Gaps = 17/253 (6%) Query: 13 FKGKRVVVLGGTSGIGFATAEAAAREGSSLVVVSSQKERADRAVSRLPEGTEGYAVDLTN 72 GK V+ GG SGIG T E EG+ +V Q E+ R P+ D+T Sbjct: 5 LNGKVAVITGGCSGIGLGTVELFVAEGACVVAADLQDEKGRMLEQRFPDQVRFARCDVTA 64 Query: 73 EEQVRQFF----SEIGEFDHLVFTAGDPLMVESLDVINVDTAHRLFNVRYWGAFMAAKYG 128 ++ + + S G D L AG S+ + + + F + G M + Sbjct: 65 DDDLAKTMALAESSFGGLDILFNNAGHGGTPASVPELTAEAWDKTFALLVRGPAMGMTHA 124 Query: 129 SRNI--RQGGSITLTSGVVGVRPQKGFTVAASICGAVESLTRALAVELSP--LRVNAVCF 184 + R GGSI T+ + G++ G +S AV ++R A ELSP +RVNA+C Sbjct: 125 LPLMQKRGGGSIINTASIAGLQAGFGPLAYSSAKAAVIHMSRCAAAELSPQKIRVNAICP 184 Query: 185 GLMRTELWD---GIPEEHRNAMYENAG----RSLPVGRVGEPEDGAEAFLYLMRE--KYS 235 GL+ T ++ G+P E + M + P+ + G PED A A LYL + ++ Sbjct: 185 GLIATSIFGASMGLPREVADQMAAQIASIGPKIQPIPKSGLPEDIAAAALYLASDDSRFV 244 Query: 236 TGQIIVVDGGSTL 248 TG IVVDGG T+ Sbjct: 245 TGTHIVVDGGITV 257 >TM0441 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 255 Score = 88.6 bits (218), Expect = 9e-18, Method: Compositional matrix adjust. Identities = 75/250 (30%), Positives = 116/250 (46%), Gaps = 18/250 (7%) Query: 13 FKGKRVVVLGGTSGIGFATAEAAAREGSSLVVVSSQKERADRAVSRLPEG----TEGYAV 68 +G+ +V GG+ G+GF A+ A G S+VV S E A A +L E T + Sbjct: 7 LRGRVALVTGGSRGLGFGIAQGLAEAGCSVVVASRNLEEASEAAQKLTEKYGVETMAFRC 66 Query: 69 DLTNEEQVRQFFSEI----GEFDHLVFTAGDPLMVESLDVINVDTAHRLFNVRYWGAFMA 124 D++N E+V++ + G+ D +V AG + +D ++ V +G + Sbjct: 67 DVSNYEEVKKLLEAVKEKFGKLDTVVNAAGINRR-HPAEEFPLDEFRQVIEVNLFGTYYV 125 Query: 125 AKYGSRNIRQGGS---ITLTSGVVGVRPQKGFTVAASICGAVESLTRALAVELSP--LRV 179 + +R+ + I + S V + A+ G V SLT+ALA E +RV Sbjct: 126 CREAFSLLRESDNPSIINIGSLTVEEVTMPNISAYAASKGGVASLTKALAKEWGRYGIRV 185 Query: 180 NAVCFGLMRTELWDGIPEEHRNAMYENAGRSLPVGRVGEPED--GAEAFLYLMREKYSTG 237 N + G RT++ + + + Y + +P+GR G PED G FL KY TG Sbjct: 186 NVIAPGWYRTKMTEAVFSDPEKLDY--MLKRIPLGRTGVPEDLKGVAVFLASEEAKYVTG 243 Query: 238 QIIVVDGGST 247 QII VDGG T Sbjct: 244 QIIFVDGGWT 253 >PAE2832 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 245 Score = 87.8 bits (216), Expect = 1e-17, Method: Compositional matrix adjust. Identities = 83/243 (34%), Positives = 126/243 (51%), Gaps = 17/243 (6%) Query: 18 VVVLGGTSGIGFATAEAAAREGSSLVVVSSQ-KERADRAVSRLPE-GTEGYAV--DLTNE 73 VVV G GIG A A AREG ++VV + + KE A+ + + E G G V D+ Sbjct: 4 VVVTGSGRGIGRAIAVRFAREGWNVVVNAKKGKEEAEETLRMVREAGGNGVVVLADVATR 63 Query: 74 EQVRQFFS----EIGEFDHLVFTAGDPLMVESLDVINVDTAHRLFNVRYWGAFMAAKYGS 129 E R G D LV AG L + SL +N D RL + + + + Y S Sbjct: 64 EGCRNVVQAAVQNFGGLDVLVNNAG--LGLYSL-FLNAD--DRLIDKQLEVSLKSVIYCS 118 Query: 130 R---NIRQGGSITLTSGVVGVRPQKGFTVAASICGAVESLTRALAVELSP-LRVNAVCFG 185 + + + GSI + + G+RP G ++ ++ A+ +LT+ALAVEL+P +RVNAV G Sbjct: 119 QEAAKVMKEGSIINIASIAGIRPFVGLSIYSAAKAAIINLTQALAVELAPRIRVNAVAPG 178 Query: 186 LMRTELWDGIPEEHRNAMYENAGRSLPVGRVGEPEDGAEAFLYLMREKYSTGQIIVVDGG 245 +++T++ D + + E + +G++ PED AEA L++ TGQ+IVVD G Sbjct: 179 VVKTKMGDSLMKLLGVTEEEFSKNHTLLGKIVAPEDVAEAVWMLVKIPTITGQVIVVDSG 238 Query: 246 STL 248 L Sbjct: 239 QLL 241 >PAE1589 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 224 Score = 86.3 bits (212), Expect = 4e-17, Method: Compositional matrix adjust. Identities = 76/240 (31%), Positives = 110/240 (45%), Gaps = 25/240 (10%) Query: 17 RVVVLGGTSGIGFATAEAAAREGSSLVVVSSQKERADRAVSRLPEGTEGYAVDLTNEEQV 76 R+VV G + GIG A A R G + +S AD Y D+ + + Sbjct: 2 RIVVTGASGGIGRALIRVAKRRGDFTIGISRSPSEADVH----------YQCDVLDLRCL 51 Query: 77 RQFFSEIGEFDHLVFTAG---DPLMVESLDVINVDTAHRLFNVRYWGAFMAAKYGSRNIR 133 + E+G D L G L V+ L ++ D +F V G+F K ++ Sbjct: 52 ERAAGEVGPVDGLALVHGHGDQRLWVKPLSELSGDDFIDVFKVDVIGSFNVVKAFLPSLT 111 Query: 134 QGGSITL---TSGVVGVRPQKGFTVAASICGAVESLTRALAVELSPLRVNAVCFGLMRTE 190 G S+ L T G++G ++VA GAV +L R+LA L+P+RVNAV FG + T Sbjct: 112 PGASVVLVSSTPGLIGDVYGIPYSVAK---GAVAALARSLAKVLAPVRVNAVAFGPIATR 168 Query: 191 LWDGIPEEHRNAMYENAGRSLPVGRVGEPEDGAEAFLYLMR--EKYSTGQIIVVDGGSTL 248 I EE G + R+G PE+ AEA +L+ Y TG ++VVDGG +L Sbjct: 169 WTGWISEEEVRGF----GERTVLKRLGTPEEAAEAIYWLLSPASSYVTGHVLVVDGGESL 224 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.134 0.384 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 17,957,308 Number of extensions: 717267 Number of successful extensions: 2754 Number of sequences better than 1.0e-05: 205 Number of HSP's gapped: 2561 Number of HSP's successfully gapped: 205 Length of query: 249 Length of database: 38,378,431 Length adjustment: 104 Effective length of query: 145 Effective length of database: 26,383,695 Effective search space: 3825635775 Effective search space used: 3825635775 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 114 (48.5 bits)