BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0599.0005.p (252 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value AGc2317 [IQR] COG1028 Dehydrogenases with different specificitie... 195 7e-50 STM1724_1 [EH] COG0512 Anthranilate/para-aminobenzoate synthases... 136 3e-32 STM1724_2 [E] COG0547 Anthranilate phosphoribosyltransferase 136 3e-32 TM1724 [IQR] COG1028 Dehydrogenases with different specificities... 136 3e-32 SA1074 [IQR] COG1028 Dehydrogenases with different specificities... 123 3e-28 >AGc2317 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 218 Score = 195 bits (495), Expect = 7e-50, Method: Compositional matrix adjust. Identities = 0/256 (0%), Positives = 0/256 (0%), Gaps = 4/256 (1%) Query: 1 MELXXXXXXXXXXVVITGGSSGIGLATAKLLVDEGAH-VLITGRTQATLDAAREQLGNNV 59 M + + + L LG + Sbjct: 37 MRNAGLTFSAIPADIDERALDEQLERDGASPEEVALELARAKALAVSALHPEALVLGCDQ 96 Query: 60 IAFLSDAGSLKDIAALADRVKVEFGTIDALFVNAGVTGFVPFESTAEETYDEIFTINAKG 119 L K + + +N+ + + + Sbjct: 97 TMALGTRVYHKPKNMAEAATHLLSLSGKVHRLNSAAVLVHNGKVVWQTVSSAELAVRTLS 156 Query: 120 PYFTVQKLAPLLSTGSGVVLTTSIVNVVGLPMLSAYAASKAALRSMTRSLARELLPRNIR 179 F + L + V + S L L +L ++ Sbjct: 157 AEFVSRHLQRVGEKALSSVGAYQLEREGIQLFTSIEGDYFTILGLPLLPLLSKLRDMDVI 216 Query: 180 VNAVSPGVIDTGILEKA--MPKEAAEQTKAQMTQQIPMLRLGDPVEVAKVVAFLAFD-AT 236 Sbjct: 217 276 Query: 237 YTTGAEFPVDGGGSQI 252 Sbjct: 277 292 >STM1724_1 [EH] COG0512 Anthranilate/para-aminobenzoate synthases component II Length = 197 Score = 136 bits (342), Expect = 3e-32, Method: Compositional matrix adjust. Identities = 0/241 (0%), Positives = 0/241 (0%), Gaps = 11/241 (4%) Query: 14 VVITGGSSGIGLATAKLLVDEGAHVLITGRTQATLDA---AREQLGNNVIAFLSDAGSLK 70 I + + + + L + + Sbjct: 8 DNIDSFTWNLADQLRTNGHNVVIYRNHIPAQTLIDRLATMKNPVLMLSPGPGVPSEAGCM 67 Query: 71 DIAALADRVKVEFGTIDALFVNAGVTGFVPFESTAEETYDEIFTINAKGPYFTVQKLAPL 130 R K+ I E + + +I G PL Sbjct: 68 PELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAGEILHGKASSIEHDGQAMFAGLANPL 127 Query: 131 L--STGSGVVLTTSIVNVVGLPMLSAYAASKAALRSMTRSLARELLPRNIRVNAVSPGVI 188 S V + A + + + + + Sbjct: 128 PVARYHSLVGSNVPAGLTINAHFNGMVMAVRHDADRVCGFQFHPESILTTQGARLLEQTL 187 Query: 189 DTGILEKAMPKEAAEQTKAQMTQQIPMLRLGDPVEVAKVVAFLAFD-ATYTTGAEFPVDG 247 + Sbjct: 188 AWAQQKL----- 242 Query: 248 G 248 Sbjct: 243 243 >STM1724_2 [E] COG0547 Anthranilate phosphoribosyltransferase Length = 334 Score = 136 bits (342), Expect = 3e-32, Method: Compositional matrix adjust. Identities = 20/241 (8%), Positives = 46/241 (19%), Gaps = 11/241 (4%) Query: 14 VVITGGSSGIGLATAKLLVDEGAHVLITGRTQATLDA---AREQLGNNVIAFLSDAGSLK 70 + + + +L L + A L + E A + + Sbjct: 8 EKLYQAQTLTQQESHQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAA 67 Query: 71 DIAALADRVKVEFGTIDALFVNAGVTGFVPFESTAEETYDEIFTINAKGPYFTVQKLAPL 130 GT + ++ F + A + L Sbjct: 68 PFPRPEYLFADIVGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAA 127 Query: 131 L--STGSGVVLTTSIVNVVGLPMLSAYAASKAALRSMTRSLARELLPRNIRVNAVSPGVI 188 + + ++ +G+ L A +M + + + Sbjct: 128 FGINLDMNADKSRQALDELGVCFLFAPKYHTGLRHAMPVRQQLKTRTLFNVLGPLINPAH 187 Query: 189 DTGILEKAMPKEAAEQTKAQMTQQIPMLRLGDPVEVAKVVAFLAFD-ATYTTGAEFPVDG 247 L + + + V T AE Sbjct: 188 PPLALIG-VYSPELVLP----IAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGE 242 Query: 248 G 248 Sbjct: 243 I 243 >TM1724 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 246 Score = 136 bits (342), Expect = 3e-32, Method: Compositional matrix adjust. Identities = 89/241 (36%), Positives = 132/241 (54%), Gaps = 11/241 (4%) Query: 14 VVITGGSSGIGLATAKLLVDEGAHVLITGRTQATLDA---AREQLGNNVIAFLSDAGSLK 70 +ITG +SGIG AT L EGA V+ ++ LD+ E L V ++ + Sbjct: 8 CLITGAASGIGKATTLLFAQEGATVIAGDISKENLDSLVKEAEGLPGKVDPYVLNVTDRD 67 Query: 71 DIAALADRVKVEFGTIDALFVNAGVTGFVPFESTAEETYDEIFTINAKGPYFTVQKLAPL 130 I + ++V ++G ID L NAG+T EE +D + +N KG + Q + P Sbjct: 68 QIKEVVEKVVQKYGRIDVLVNNAGITRDALLVRMKEEDWDAVINVNLKGVFNVTQMVVPY 127 Query: 131 L--STGSGVVLTTSIVNVVGLPMLSAYAASKAALRSMTRSLARELLPRNIRVNAVSPGVI 188 + +V +S+V + G P + YAASKA + MT++ A+EL RNIRVNAV+PG I Sbjct: 128 MIKQRNGSIVNVSSVVGIYGNPGQTNYAASKAGVIGMTKTWAKELAGRNIRVNAVAPGFI 187 Query: 189 DTGILEKAMPKEAAEQTKAQMTQQIPMLRLGDPVEVAKVVAFLAFD-ATYTTGAEFPVDG 247 +T + EK +P++A E +IP+ R G P EVA+V+ FLA D ++Y TG +DG Sbjct: 188 ETPMTEK-LPEKARETA----LSRIPLGRFGKPEEVAQVILFLASDESSYVTGQVIGIDG 242 Query: 248 G 248 G Sbjct: 243 G 243 >SA1074 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 246 Score = 123 bits (308), Expect = 3e-28, Method: Compositional matrix adjust. Identities = 83/241 (34%), Positives = 129/241 (53%), Gaps = 12/241 (4%) Query: 15 VITGGSSGIGLATAKLLVDEGAHVLIT-GRTQATLDAAREQL---GNNVIAFLSDAGSLK 70 ++TG S GIG + A L +EG +V + ++ +A E++ G + A ++ Sbjct: 8 LVTGASRGIGRSIALQLAEEGYNVAVNYAGSKEKAEAVVEEIKAKGVDSFAIQANVADAD 67 Query: 71 DIAALADRVKVEFGTIDALFVNAGVTGFVPFESTAEETYDEIFTINAKGPYFTVQKLAP- 129 ++ A+ V +FG++D L NAG+T E+ +D++ N KG + +QK P Sbjct: 68 EVKAMIKEVVSQFGSLDVLVNNAGITRDNLLMRMKEQEWDDVIDTNLKGVFNCIQKATPQ 127 Query: 130 LLSTGSGVVLT-TSIVNVVGLPMLSAYAASKAALRSMTRSLARELLPRNIRVNAVSPGVI 188 +L SG ++ +S+V VG P + Y A+KA + +T+S AREL R I VNAV+PG I Sbjct: 128 MLRQRSGAIINLSSVVGAVGNPGQANYVATKAGVIGLTKSAARELASRGITVNAVAPGFI 187 Query: 189 DTGILEKAMPKEAAEQTKAQMTQQIPMLRLGDPVEVAKVVAFLAFD-ATYTTGAEFPVDG 247 + M +++ K QM QIP+ R G ++A VAFLA D A Y TG V+G Sbjct: 188 VSD-----MTDALSDELKEQMLTQIPLARFGQDTDIANTVAFLASDKAKYITGQTIHVNG 242 Query: 248 G 248 G Sbjct: 243 G 243 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.133 0.360 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 16,148,654 Number of extensions: 627904 Number of successful extensions: 4866 Number of sequences better than 1.0e-05: 353 Number of HSP's gapped: 4345 Number of HSP's successfully gapped: 353 Length of query: 252 Length of database: 38,378,431 Length adjustment: 104 Effective length of query: 148 Effective length of database: 26,383,695 Effective search space: 3904786860 Effective search space used: 3904786860 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 114 (48.5 bits)