BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0599.0004.p (299 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value STM2445 [IQR] COG1028 Dehydrogenases with different specificitie... 64 3e-10 L121175 [IQR] COG1028 Dehydrogenases with different specificitie... 62 8e-10 Ta0191 [IQR] COG1028 Dehydrogenases with different specificities... 61 2e-09 AGl2880 [R] COG4618 ABC-type protease/lipase transport system, A... 60 6e-09 MA0107 [IQR] COG1028 Dehydrogenases with different specificities... 59 9e-09 >STM2445 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 263 Score = 63.9 bits (154), Expect = 3e-10, Method: Compositional matrix adjust. Identities = 60/197 (30%), Positives = 96/197 (48%), Gaps = 25/197 (12%) Query: 23 ALITGASQGIGLALARKLLSQNWQVITLNRSDFPADDMLIQNALKNGWLRAYKVK-DLTD 81 ALITGASQGIG +AR +I L+ SD + + + L R VK D+ D Sbjct: 9 ALITGASQGIGEGIARVFARHGANLILLDISD---EIEKLADELGGRGHRCTAVKADVRD 65 Query: 82 YGSLRRSLEEIKSKEQRIDILFNNAG----GGLSELRYSKQGREMHYEL-LTVVPYIILM 136 + S++ ++ K E RIDIL NNAG G L S++ R+ H ++ + V + Sbjct: 66 FASVQAAVARAKETEGRIDILVNNAGVCRLGNF--LDMSEEDRDFHIDINIKGVWNVTKA 123 Query: 137 ELKELLKNGSLKTVINTSSQVLRFTKEFSIENLEHPKTFRKMYGPYATSKLALSLWTQAV 196 L E++K + V+ +S T + + E YA SK A+ T+++ Sbjct: 124 VLPEMIKRKDGRIVMMSS-----VTGDMVADPGET---------AYALSKAAIVGLTKSL 169 Query: 197 APQLAKEGIKIRSVDPG 213 A + A+ GI++ ++ PG Sbjct: 170 AVEYAQSGIRVNAICPG 186 >L121175 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 339 Score = 62.4 bits (150), Expect = 8e-10, Method: Compositional matrix adjust. Identities = 65/231 (28%), Positives = 99/231 (42%), Gaps = 21/231 (9%) Query: 15 NTNQREHIALITGASQGIGLALARKLLSQNWQVITLNRSDFPADDMLIQNALKNGWLRAY 74 N N +ITG GIGL + ++L ++I PA M + A KN Sbjct: 24 NINLSGKTIIITGGYSGIGLEMTKQLSRAGAKII------IPARRMEV--AKKN----LS 71 Query: 75 KVK-------DLTDYGSLRRSLEEIKSKEQRIDILFNNAGGGLSELRYSKQGREMHYELL 127 K+K DLT+ S+ + E ++DIL N+AG L+ +G E H+ Sbjct: 72 KIKNIDILPLDLTNPESIHQFAESFLQNNSKLDILINSAGLMYLPLKRDSRGIEYHFATN 131 Query: 128 TVVPYIILMELKELLKNGSLKTVINTSSQVLRFTKEFSIENLEHPKTFRKMYGPYATSKL 187 + Y + +EL LKN + VIN SS+ + T N E + + G Y SK Sbjct: 132 HLGHYQLTLELLPALKNAKISRVINLSSRAQQMTPLLEDWNFEKIENYNPQIG-YQQSKT 190 Query: 188 ALSLWTQAVAPQLAKEGIKIRSVDPGINNTLRKGK-DSGLTAWFELFMKVF 237 A L++ + K GI+ +V PG+ G+ +S +T W F Sbjct: 191 ANVLFSIKLDELGKKYGIRSFAVHPGMIPMTNIGRQNSPITPWLRYLADYF 241 >Ta0191 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 268 Score = 60.8 bits (146), Expect = 2e-09, Method: Compositional matrix adjust. Identities = 55/209 (26%), Positives = 91/209 (43%), Gaps = 17/209 (8%) Query: 8 EGGGHPLNTNQREHIALITGASQGIGLALARKLLSQNWQVITLNRSDFPADDMLIQNALK 67 E G + N IAL+TG+S G+G A+A+ + +V RS D ++ Sbjct: 3 ENRGSRIAINLTGKIALVTGSSSGLGAAIAKYMARAGAKVAVHYRSGKDRADAIVDEIKN 62 Query: 68 NGWLRAYKVKDLTDYGSLRRSLEEIKSKEQRIDILFNNAG-GGLSEL--RYSKQGREMHY 124 +G D++ +++ EI SK +DIL NNAG G EL E Sbjct: 63 DGGFAMAFYGDVSKKEDVQKLFSEIDSKLGTVDILVNNAGIDGKRELVGEDDPDDWEKVI 122 Query: 125 ELLTVVPYIILMELKELLKNGSLKTVINTSSQVLRFTKEFSIENLEHPKTFRKMYGPYAT 184 E+ + PY E + +K +IN +S H Y Y++ Sbjct: 123 EVNLMGPYYCAREAVKRMKPKKSGVIINITSV--------------HEYVPWSGYTAYSS 168 Query: 185 SKLALSLWTQAVAPQLAKEGIKIRSVDPG 213 +K LS++T+A+A +L+ I++ ++ PG Sbjct: 169 AKAGLSMFTKALAQELSDYNIRVVAIAPG 197 >AGl2880 [R] COG4618 ABC-type protease/lipase transport system, ATPase and permease components Length = 249 Score = 59.7 bits (143), Expect = 6e-09, Method: Compositional matrix adjust. Identities = 54/206 (26%), Positives = 93/206 (45%), Gaps = 34/206 (16%) Query: 22 IALITGASQGIGLALARKLLSQNWQVITLNRSDFPADDMLIQNALKNGWLRAYKVKDLTD 81 IA+ITGAS+G+G A+AR ++ V+ S A + +++ + G D+++ Sbjct: 9 IAVITGASKGLGAAIARSFAAEGASVVVNYSSSKSAAEAVVEEIIDAGGTAIAVKADMSE 68 Query: 82 YGSLRRSLEEIKSKEQRIDILFNNAGGGLSELRYSKQGRE--------MHYELLTVVPYI 133 + + R+DIL NNAG Y Q E H E L V Y+ Sbjct: 69 PAEVEELFAATNAAFGRVDILVNNAG------VYDFQPLENLSIDLFRKHME-LNVFGYL 121 Query: 134 ILME--LKELLKNGSLKTVINTSSQVLRFTKEFSIENLEHPKTFRKMYGPYATSKLALSL 191 + ++ +K + + GS ++N SS V F E + Y SK A+ Sbjct: 122 LAIKEAVKYMAEGGS---IVNMSSTVTIFGPENA--------------SVYTASKGAIDG 164 Query: 192 WTQAVAPQLAKEGIKIRSVDPGINNT 217 T+A++ +LA I++ ++ PG+ +T Sbjct: 165 LTRALSNELAPRKIRVNAIKPGVVDT 190 >MA0107 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 255 Score = 58.9 bits (141), Expect = 9e-09, Method: Compositional matrix adjust. Identities = 52/196 (26%), Positives = 94/196 (47%), Gaps = 19/196 (9%) Query: 22 IALITGASQGIGLALARKLLSQNWQVITLNRSDFPADDMLIQNAL-KNGWLRAYKVKDLT 80 +A++TGAS G+G+ AR L +Q + + R + + +Q + K G Y + D+ Sbjct: 11 VAIVTGASSGLGVEFARALANQGANIAIIARREEKLKE--VQKEIEKLGVTCRYYLCDVM 68 Query: 81 DYGSLRRSLEEIKSKEQRIDILFNNAGGGLSELRYSKQGREMHYELLTV---VPYIILME 137 ++ ++E++ RIDIL NNAG GL + K EM + ++ Y E Sbjct: 69 KTEQIKNAVEQVVKDFGRIDILVNNAGLGLVDAA-DKTTDEMWHNMIDTNLNGVYFFARE 127 Query: 138 LKELLKNGSLKTVINTSSQVLRFTKEFSIENLEHPKTFRKMYGPYATSKLALSLWTQAVA 197 + +++ +IN S S++ L P T Y ++K + + T+++A Sbjct: 128 VGKIMLRQKHGRIINIGS----IHSTVSMKGL--PVT------AYCSTKGGVLMLTKSLA 175 Query: 198 PQLAKEGIKIRSVDPG 213 + AKEGI + ++ PG Sbjct: 176 TEWAKEGITVNAIGPG 191 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.136 0.388 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 21,272,881 Number of extensions: 829209 Number of successful extensions: 2280 Number of sequences better than 1.0e-05: 68 Number of HSP's gapped: 2334 Number of HSP's successfully gapped: 68 Length of query: 299 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 193 Effective length of database: 26,153,027 Effective search space: 5047534211 Effective search space used: 5047534211 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 115 (48.9 bits)