BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0598.0027.p (467 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value STM0042 [G] COG2211 Na+/melibiose symporter and related transpor... 325 1e-88 CAC3422 [G] COG2211 Na+/melibiose symporter and related transpor... 284 2e-76 BS_ynaJ [G] COG2211 Na+/melibiose symporter and related transpor... 254 2e-67 CAC3451 [G] COG2211 Na+/melibiose symporter and related transpor... 248 1e-65 BS_ydjD [G] COG2211 Na+/melibiose symporter and related transpor... 233 4e-61 >STM0042 [G] COG2211 Na+/melibiose symporter and related transporters Length = 457 Score = 325 bits (832), Expect = 1e-88, Method: Compositional matrix adjust. Identities = 165/443 (37%), Positives = 270/443 (60%), Gaps = 1/443 (0%) Query: 19 NSNQKIPLLEKISYGLGDTGSNLIYTVITTYLTFYFTDIYGIGAAAVGTLMLIARLVDMI 78 + + + LL++ISYG D NL+Y +TY+ +++TD+ GI A G ++L+AR++D I Sbjct: 11 SGSASLSLLQRISYGSLDVAGNLLYCFSSTYILYFYTDVAGISLAVAGVILLLARIIDGI 70 Query: 79 DSPILGILIDKTNTRWGKSRPWILWSCIPFSIVSILLFMGPELSASGKMAYAYIFYIAAN 138 D+PI GI+IDKT +R+GK RPW LW +PF++ S L F P++S +GK YA I Y+ A+ Sbjct: 71 DAPIWGIIIDKTRSRYGKCRPWFLWLPLPFAVFSALSFWSPDISMTGKAIYAAISYMIAS 130 Query: 139 VLYAAVNNPLQTMLPSMTSNIQEKTVANTFRMFGGQIGGLIVNLTLLPLVAYLGAGDDRK 198 +L+ +N PL +LP MT + +E+ V N++RM GGQIG L++N T LPLVA+LG GDD Sbjct: 131 ILFTGLNTPLSAILPLMTLSPKERLVLNSWRMTGGQIGVLLMNATALPLVAFLGNGDDHA 190 Query: 199 GFFYAMIIFSLTALVLFFITFFNTRE-RVQGVSGNEAIPVKEGIKAIKGNLPWIGICLFG 257 GF Y I F++ + L F N RE + +P+K+ A+KGN PWI + L Sbjct: 191 GFIYTAITFAIISCALTLFAFKNIREMDTDKIQHEPKLPMKKSFAAMKGNWPWILMVLAN 250 Query: 258 ITMYTMFIMRLSAAIYYMTYYIEKPEIISTVNTIAMSSLLGILAVPFLTKKFSKKSLVIT 317 + + R + +YY+TY +++ +++ +N++A +L I+A+PF +K +K + + Sbjct: 251 LIFWIALQQRNTTIVYYLTYNLDRKDLVPLINSLATIQILFIIAIPFFSKYLAKTWIWVG 310 Query: 318 GLAINIVGQLIIFMAGSNVALIIIGTIVGSVGMGLPSGLLFVLKADTVDYGEWKSGVRAP 377 GL + G +++++A N+ +I I+G++G G+ + F + VD+G WK+G++A Sbjct: 311 GLLVATFGGVMMWLAADNITFLIAAWILGNIGSGIACSMPFAMLGFAVDFGAWKTGIKAT 370 Query: 378 GXXXXSASGVANKLGSGLGSAIPVWLLAAGGYVAKQQQTPEALRMIALSYIWLPVILGAV 437 G S K+GSGLG+A W++ + GYV Q+ L I ++IW P +L A+ Sbjct: 371 GILIAFGSTFCIKMGSGLGTAFAAWIMNSFGYVPNHAQSAAGLEGIIWAFIWAPALLFAL 430 Query: 438 AILVMVFVYKWEKPHAEIVAELE 460 A + ++F K+E +I +LE Sbjct: 431 AAIPLLFFRKYEAMEEKIRHDLE 453 >CAC3422 [G] COG2211 Na+/melibiose symporter and related transporters Length = 445 Score = 284 bits (727), Expect = 2e-76, Method: Compositional matrix adjust. Identities = 167/441 (37%), Positives = 267/441 (60%), Gaps = 6/441 (1%) Query: 23 KIPLLEKISYGLGDTGSNLIYTVITTYLTFYFTDIYGIGAAAVGTLMLIARLVDMIDSPI 82 KIPL+EKISYGLGDT S L + +++ Y+ F++TD++G+ A++ L+L AR++D + PI Sbjct: 5 KIPLVEKISYGLGDTASILTFQMVSVYIMFFYTDVFGLSTASIAVLLLSARIIDALIDPI 64 Query: 83 LGILIDKTNTRWGKSRPWILWSCIPFSIVSILLFMGPELSASGKMAYAYIFYIAANVLYA 142 +GI+IDKT+T+WGK RP+ LW +P+ +++I F P LS +GK+ YAYI YI+ N++Y+ Sbjct: 65 IGIIIDKTSTKWGKCRPYFLWFSVPYGLIAIATFFTPHLSTNGKLIYAYITYISLNIVYS 124 Query: 143 AVNNPLQTMLPSMTSNIQEKTVANTFRMFGGQIGGLIVNLTLLPLVAYLGAGDDRKGFFY 202 +N P+ +LPS+T + +E+T R+F G +IV+ LPLV+ LG G+ +KGFF Sbjct: 125 IINLPITAILPSLTDDYKERTQTTAIRIFLASCGSIIVSTFTLPLVSILGEGNKQKGFFL 184 Query: 203 AMIIFSLTALVLFFITFFNTRERVQGVSGNEAIPVKEGIKAIKGNLPWIGICLFGITMYT 262 M++F A +LF TF T ER+ GN IP+K+ I +K NLPW+ I L + Y Sbjct: 185 TMVLFGFIASLLFVNTFIQTHERILANKGNP-IPLKKSIMTLK-NLPWLLITLVNVFTYI 242 Query: 263 MFIMRLSAAIYYMTYYIEKPEIISTVNTIAMSSLLGILAV-PFLTKKFSKKSLVITGLAI 321 ++ S+ +YY++Y I + ++I ++ +L +A+ P L K K++ +I G + Sbjct: 243 GLNIKNSSMVYYLSYNISRQDLIQIFMFVSTIGVLPAIALTPILVTKIGKRNTLIVG-TL 301 Query: 322 NIVGQLIIFMAGSNVALIIIGTIVGSVGMGLPSGLLFVLKADTVDYGEWKSGVRAPGXXX 381 ++ +F SNV L++I + ++G G + + F + D VDYGEWK+GVRA Sbjct: 302 FVLISCFLFAISSNVILLLIADGISALGSGFSNAVTFSMLPDIVDYGEWKTGVRAQ-GLL 360 Query: 382 XSASGVANKLGSGLGSAIPVWLLAAGGYVAKQQQTPEALRMIALSYIWLPVILGAVAILV 441 S+S +K G G+GS + +L+ G YV Q A I+ ++IW I + + V Sbjct: 361 SSSSSFGSKFGMGVGSGLAALILSLGHYVPHASQAYSAKFAISFNFIWASAITSGIILFV 420 Query: 442 MVFVYKWEKPHAEIVAELEAR 462 + F YK +K + I A L + Sbjct: 421 LPF-YKIDKLYPMIQAYLSKK 440 >BS_ynaJ [G] COG2211 Na+/melibiose symporter and related transporters Length = 85 Score = 254 bits (649), Expect = 2e-67, Method: Compositional matrix adjust. Identities = 0/445 (0%), Positives = 0/445 (0%), Gaps = 5/445 (1%) Query: 22 QKIPLLEKISYGLGDTGSNLIYTVITTYLTFYFTDIYGIGAAAVGTLMLIARLVDMIDSP 81 + + + L T + ++ + ++ + Sbjct: 7 KSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIPLSEWTTSMYVIQSSMIFVYSLV 66 Query: 82 ILGILIDKTNTRWGKSRPWILWSCIPFSIVSILLFMGPELSASGKMAYAYIFYIAANVLY 141 Sbjct: 67 126 Query: 142 AAVNNPLQTMLPSMTSNIQEKTVANTFRMFGGQIGGLIVNLTLLPLVAYLG--AGDDRKG 199 Sbjct: 127 186 Query: 200 FFYAMIIFSLTALVLFFITFFNTRERVQGVSGNEAIPVKEGIKAIKGNLPWIGICLFGIT 259 Sbjct: 187 246 Query: 260 MYTMFIMRLSAAIYYMTYYIEKPEIISTVNTIAMSSLLGILA-VPFLTKKFSKKSLVITG 318 Sbjct: 247 306 Query: 319 LAINIVGQL-IIFMAGSNVALIIIGTIVGSVGMGLPSGLLFVLKADTVDYGEWKSGVRAP 377 Sbjct: 307 - 365 Query: 378 GXXXXSASGVANKLGSGLGSAIPVWLLAAGGYVAKQQQTPEALRMIALSYIWLPVILGAV 437 Sbjct: 366 425 Query: 438 AILVMVFVYKWEKPHAEIVAELEAR 462 Sbjct: 426 450 >CAC3451 [G] COG2211 Na+/melibiose symporter and related transporters Length = 458 Score = 248 bits (634), Expect = 1e-65, Method: Compositional matrix adjust. Identities = 143/443 (32%), Positives = 247/443 (55%), Gaps = 4/443 (0%) Query: 23 KIPLLEKISYGLGDTGSNLIYTVITTYLTFYFTDIYGIGAAAVGTLMLIARLVDMIDSPI 82 KI + EKI Y GD SNLIY I+ YL F++T+++G+ A G + L+ R +D I+ PI Sbjct: 6 KIKMREKIGYASGDLASNLIYQTISIYLLFFYTNVFGLSAGQAGVMFLVVRFIDAINDPI 65 Query: 83 LGILIDKTNTRWGKSRPWILWSCIPFSIVSILLFMGPELSASGKMAYAYIFYIAANVLYA 142 +G L+DKTNTR+G+ RP++L+ PF++++ L F P SA+GK+ YAY+ Y+ ++ Y Sbjct: 66 IGTLVDKTNTRFGRFRPYLLYGAAPFAVLAFLCFTTPNFSATGKLIYAYVTYVGLSITYT 125 Query: 143 AVNNPLQTMLPSMTSNIQEKTVANTFRMFGGQIGGLIVNLTLLPLVAYLGAGDDRK-GFF 201 +N P + ++T + QE + RMF +GG+IV+ + L AY G+ Sbjct: 126 CINVPYGALTSAITDDNQEIVSLTSVRMFFANLGGVIVSYFVPVLSAYFTKSFGLSGGWQ 185 Query: 202 YAMIIFSLTALVLFFITFFNTRERVQGVSGNEAIPVKEGIKAIKGNLPWIGICLFGITMY 261 M I + L F +T+ERV+ V+ + I + + K N P I + +F + ++ Sbjct: 186 ITMSILGIAGAFLLLFCFSSTKERVKSVNQDHKIKFSDLFEQFKTNRPLIVLSIFFVLIF 245 Query: 262 TMFIMRLSAAIYYMTYYIEKPEIISTVNTI-AMSSLLGILAVPFLTKKFSKKSLVITGLA 320 + + S IYY+TY + + +++ + ++ + + I +P + +K KK L+I+ L Sbjct: 246 GINSINSSIGIYYITYNVGRADLVQWYTVLGSLPAFVCIPLIPKINRKIGKKPLLISSLL 305 Query: 321 INIVGQLIIFMAGSN-VALIIIGTIVGSVGMGLPSGLLFVLKADTVDYGEWKSGVRAPGX 379 I ++G L + + ++ VALI++ ++ S+G ++ L +T++YGE+ +G R G Sbjct: 306 ITVLGTLSLLVIPTHAVALILVSRVITSIGSLTAGAFMWSLIPETIEYGEYTTGKRLSG- 364 Query: 380 XXXSASGVANKLGSGLGSAIPVWLLAAGGYVAKQQQTPEALRMIALSYIWLPVILGAVAI 439 + G K G LG A+P +L GYVA + QTP AL I L+ +P +L +A+ Sbjct: 365 LIYAIIGFFFKCGMALGGAVPGIILGNFGYVANKTQTPHALTGILLTATVVPAVLMILAL 424 Query: 440 LVMVFVYKWEKPHAEIVAELEAR 462 + + F +K + I+A L+ R Sbjct: 425 IDITFYNLDDKKYNHIIATLKER 447 >BS_ydjD [G] COG2211 Na+/melibiose symporter and related transporters Length = 463 Score = 233 bits (595), Expect = 4e-61, Method: Compositional matrix adjust. Identities = 144/461 (31%), Positives = 236/461 (51%), Gaps = 4/461 (0%) Query: 4 MGVKVNDGLAGSQTMNSNQKIPLLEKISYGLGDTGSNLIYTVITTYLTFYFTDIYGIGAA 63 +G + L S+ + E+I YG GD N+I+ + YL FY+TD+ GI A Sbjct: 2 IGTEKVTALKAKHQSKSSTGLSWFERIGYGFGDMSYNIIFQFVNAYLLFYYTDVGGIQPA 61 Query: 64 AVGTLMLIARLVDMIDSPILGILIDKTNTRWGKSRPWILWSCIPFSIVSILLFMGPELSA 123 + TL L+ R++D I PI+G+++DKTNTRWGK+RP++LW PF++ + L F P Sbjct: 62 VIATLFLVVRVLDAIFDPIMGLILDKTNTRWGKARPYLLWVAFPFALFTFLCFTTPHFGE 121 Query: 124 SGKMAYAYIFYIAANVLYAAVNNPLQTMLPSMTSNIQEKTVANTFRMFGGQIGGLIVNLT 183 +G M YAY+ YI + ++ P+ ++ MT++++E+TV T RM IG L+ Sbjct: 122 TGNMVYAYVTYILLGMSFSMQTIPVNSLTGRMTNSVEERTVLTTTRMILVYIGILLSISC 181 Query: 184 LLPLVAYLGAGDDRKGFFYAMIIFSLTALVLFFITFFNTRERVQGVSGNEAIPVKEGIKA 243 PL +G D GF +I++ ++VL +FF RER+Q + K + Sbjct: 182 ATPLATAIGGEDQAFGFQMTALIYAAVSIVLNLFSFFTVRERIQPKKRKKQGIKKT-LSV 240 Query: 244 IKGNLPWIGICLFGITMYTMFIMRLSAAIYYMTYYIEKPEIISTVNTIAM-SSLLGILAV 302 + N P + + + F ++LS +YY TY + E + + ++L+ L + Sbjct: 241 LFKNKPLLMLISSFLAFAIGFNIKLSTMVYYFTYNVNHKEFVFMGTVLFFGAALISNLFI 300 Query: 303 PFLTKKFSKKSLVITGLAINIVGQL-IIFMAGSNVALIIIGTIVGSVGMGLPSGLLFVLK 361 PF ++K+ +K ++I A++++ + F S++ LI I + L + + Sbjct: 301 PFFSEKWGRKQVMIITAALSLISYAGLHFTPYSSIPLIFIWLFASGFFTTPLNTLAWGMV 360 Query: 362 ADTVDYGEWKSGVRAPGXXXXSASGVANKLGSGLGSAIPVWLLAAGGYVAKQQQTPEALR 421 AD VDY EWK+G+RA G S S + NKLG L + L GYVA QT +L Sbjct: 361 ADCVDYAEWKTGIRADGVVISSMSFI-NKLGVALAGSFSAIYLGIAGYVANTDQTVASLN 419 Query: 422 MIALSYIWLPVILGAVAILVMVFVYKWEKPHAEIVAELEAR 462 I +P ++I+++ F EK + I++ELE R Sbjct: 420 AIKNMNALIPGFFILLSIILIAFYPLTEKRYKHIISELEQR 460 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.324 0.140 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 33,590,608 Number of extensions: 1400463 Number of successful extensions: 5291 Number of sequences better than 1.0e-05: 46 Number of HSP's gapped: 5149 Number of HSP's successfully gapped: 46 Length of query: 467 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 357 Effective length of database: 25,691,691 Effective search space: 9171933687 Effective search space used: 9171933687 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.5 bits) S2: 118 (50.1 bits)