BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0598.0021.p (379 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value MK0782 [CE] COG0473 Isocitrate/isopropylmalate dehydrogenase 230 3e-60 RSp1612 [CE] COG0473 Isocitrate/isopropylmalate dehydrogenase 223 6e-58 MJ0720 [CE] COG0473 Isocitrate/isopropylmalate dehydrogenase 221 1e-57 PAB2424 [CE] COG0473 Isocitrate/isopropylmalate dehydrogenase 219 8e-57 MA0201 [CE] COG0473 Isocitrate/isopropylmalate dehydrogenase 216 5e-56 >MK0782 [CE] COG0473 Isocitrate/isopropylmalate dehydrogenase Length = 335 Score = 230 bits (587), Expect = 3e-60, Method: Compositional matrix adjust. Identities = 151/367 (41%), Positives = 202/367 (55%), Gaps = 47/367 (12%) Query: 4 YNISVLAGDGIGPEVTEEALKVLRAVQRNYGSXXXXXXXXQNGASSGQFLQMGKAMTEEQ 63 Y I+V+ GDGIGPEV E AL V+ + + G + + G + E+ Sbjct: 3 YKIAVIPGDGIGPEVIEAALHVIEPL------IDAEFVEGEAGDECAE--KHGDPLPEDT 54 Query: 64 FGQAKAADAMLMGAIGLFEARHPDGREVNGDVIFRLRFDLDLYAGVRPVKLYEGVPGPLR 123 ADA+L GA G E DVI RLR +LDLYA +RPV+ G PG LR Sbjct: 55 LELCHEADAILFGAAG----------ETAADVIVRLRQELDLYANIRPVR---GFPG-LR 100 Query: 124 EQ------EKGIDYVVVRENIEGLYASRTGGCNVRDEIATDTIIITRTGTQKIVDYXXXX 177 E +D+V+VREN EGLY+ G RD A IIT GT++I + Sbjct: 101 ELTGEPYVRDDVDFVIVRENTEGLYSGIEG--RFRD-TAYTLRIITEEGTRRIAEVACDL 157 Query: 178 XXXXXXXXXXXXSMVTCVDKANVL-SSYAYFRKIYDEVAANYPDIAKDYAYVDAMAFYQV 236 + VTCV KANV+ + FR++ EV + ++Y YVDA A + + Sbjct: 158 AEERGS------NTVTCVHKANVMRETCGLFREVCKEVVESRGLEFEEY-YVDAAAMFMI 210 Query: 237 IQPAQYDVLVAENMFADILSDLSSATIGGLGLAPSGDIGDRHAMFQPSHGSAPGIAGKQI 296 +P ++DV+V NMF DILSD ++A +GGLGLAPSG++GDRH +F+P HGSAP IAGK I Sbjct: 211 TEPERFDVVVTPNMFGDILSDEAAALVGGLGLAPSGNVGDRHGLFEPVHGSAPDIAGKGI 270 Query: 297 ANPIATILSGGMMLDWLGEQHQDNALIQAGKDIEAAVAAVLKEGTIRTGDIGGKSSTSEV 356 ANP ATILS MML+WLGE +A + + AV ++EG + T D+GG T EV Sbjct: 271 ANPFATILSAVMMLEWLGED-------EAAEAVREAVGEAIREGVV-TPDLGGDKKTMEV 322 Query: 357 GDAVVQA 363 + V +A Sbjct: 323 AEFVREA 329 >RSp1612 [CE] COG0473 Isocitrate/isopropylmalate dehydrogenase Length = 361 Score = 223 bits (567), Expect = 6e-58, Method: Compositional matrix adjust. Identities = 148/374 (39%), Positives = 211/374 (56%), Gaps = 24/374 (6%) Query: 1 MSR--YNISVLAGDGIGPEVTEEALKVLRAVQRNYGSXXXXXXXXQNGASSGQFLQMGKA 58 M+R Y I+V+ GDGIG EV E L+VL A R +G AS +LQ G+ Sbjct: 1 MTRKTYRIAVIPGDGIGREVMPEGLRVLDAAARRFGIDLEYQHI--EWASCDYYLQHGRM 58 Query: 59 MTEEQFGQAKAADAMLMGAIGLFEARHPDGREVNGDVIFRLRFDLDLYAGVRPVKLYEGV 118 M ++ Q + ADA+L GA+G + A PD + G ++ + R + D Y +RPV+L+EGV Sbjct: 59 MPDDWKAQLQGADAILFGAVG-WPATVPDHISLWGSLL-KFRREFDQYINLRPVRLFEGV 116 Query: 119 PGPLREQEKG-IDYVVVRENIEGLYASRTGGC---NVRDEIATDTIIITRTGTQKIVDYX 174 P PL ++ G IDY VVREN EG Y S GG E+ + +R G ++++ + Sbjct: 117 PCPLANRKPGDIDYYVVRENTEGEYTS-LGGIMYEGTDREVVIQESVYSRKGAERLLKFA 175 Query: 175 XXXXXXXXXXXXXXXSMVTCVDKANVLS-SYAYFRKIYDEVAANYPDIAKDYAYVDAMAF 233 VT K+N ++ S ++ DEVA +YP++ D ++D + Sbjct: 176 FDLARSRAR------KHVTLATKSNGIAISMPWWDARADEVATHYPEVTLDKQHIDILTA 229 Query: 234 YQVIQPAQYDVLVAENMFADILSDLSSATIGGLGLAPSGDIG-DR--HAMFQPSHGSAPG 290 V+QP ++DV+ A N+F DILSDL AT G +GLAPS ++ DR ++F+P HGSAP Sbjct: 230 RFVLQPGRFDVVAATNLFGDILSDLGPATTGTIGLAPSANLNPDRTFPSLFEPVHGSAPD 289 Query: 291 IAGKQIANPIATILSGGMMLDWLGEQHQDNALIQAGKDIEAAVAAVLKEGTIRTGDIGGK 350 I GK IANPIA I S +MLD+L E A A I AA+ AVLK G RT D+GG Sbjct: 290 IYGKNIANPIAMIWSAALMLDFLTEGQ--GAGRAAHDAIVAAIEAVLKGGP-RTPDLGGS 346 Query: 351 SSTSEVGDAVVQAL 364 ++T++VG+A+ L Sbjct: 347 ANTTQVGEAIASHL 360 >MJ0720 [CE] COG0473 Isocitrate/isopropylmalate dehydrogenase Length = 333 Score = 221 bits (564), Expect = 1e-57, Method: Compositional matrix adjust. Identities = 149/368 (40%), Positives = 198/368 (53%), Gaps = 40/368 (10%) Query: 4 YNISVLAGDGIGPEVTEEALKVLRAVQRNYGSXXXXXXXXQNGASSGQFLQMGKAMTEEQ 63 + I V+ GDGIG EV ++VL A + A + + GKA+ EE Sbjct: 2 HKICVIEGDGIGKEVVPATIQVLEATGLPFEFVYAE-------AGDEVYKRTGKALPEET 54 Query: 64 FGQAKAADAMLMGAIGLFEARHPDGREVNGDVIFRLRFDLDLYAGVRPVKLYEGVPGPLR 123 A DA+L GA G E DVI +LR LD YA +RPVK Y+GV LR Sbjct: 55 IETALDCDAVLFGAAG----------ETAADVIVKLRHILDTYANIRPVKAYKGVKC-LR 103 Query: 124 EQEKGIDYVVVRENIEGLYASRTGGCNVRDEIATDTIIITRTGTQKIVD--YXXXXXXXX 181 IDYV+VREN EGLY + EI I TR T+K + + Sbjct: 104 PD---IDYVIVRENTEGLYKG------IEAEIDEGITIATRVITEKACERIFRFAFNLAR 154 Query: 182 XXXXXXXXSMVTCVDKANVLS-SYAYFRKIYDEVAANYPDI-AKDYAYVDAMAFYQVIQP 239 VTC KANVL + F+KI+ +VA Y DI A+DY Y+DAM Y + +P Sbjct: 155 ERKKMGKEGKVTCAHKANVLKLTDGLFKKIFYKVAEEYDDIKAEDY-YIDAMNMYIITKP 213 Query: 240 AQYDVLVAENMFADILSDLSSATIGGLGLAPSGDIGDRHAMFQPSHGSAPGIAGKQIANP 299 +DV+V N+F DILSD ++ T+GGLGLAPS +IGD H +F+P HGSAP IAGK+IANP Sbjct: 214 QVFDVVVTSNLFGDILSDGAAGTVGGLGLAPSANIGDEHGLFEPVHGSAPDIAGKKIANP 273 Query: 300 IATILSGGMMLDWLGEQHQDNALIQAGKDIEAAVAAVLKEGTIRTGDIGGKSSTSEVGDA 359 ATILS +ML +LGE +A +E A+ VL G + T D+GG +T E+ + Sbjct: 274 TATILSAVLMLRYLGEY-------EAADKVEKALEEVLALG-LTTPDLGGNLNTFEMAEE 325 Query: 360 VVQALTKQ 367 V + + ++ Sbjct: 326 VAKRVREE 333 >PAB2424 [CE] COG0473 Isocitrate/isopropylmalate dehydrogenase Length = 354 Score = 219 bits (557), Expect = 8e-57, Method: Compositional matrix adjust. Identities = 147/367 (40%), Positives = 203/367 (55%), Gaps = 28/367 (7%) Query: 3 RYNISVLAGDGIGPEVTEEALKVLRAVQRNYGSXXXXXXXXQNGASSGQFLQMGKAMTEE 62 + I+V+ GDGIG EV E LKVL+ ++ GA +L+ G+ + + Sbjct: 2 KLRIAVIPGDGIGKEVVAEGLKVLKKLE-ELSRVSFEFKEYPFGAE--HYLKTGETLPDW 58 Query: 63 QFGQAKAADAMLMGAIGLFEARHPDGREVNGDVIFRLRFDLDLYAGVRPVKLYEGVPGPL 122 + K DA+ GAIG + R G G ++ ++RF+LDLY +RPVKLY PL Sbjct: 59 AIEEFKKFDAIYFGAIG--DPRVKPGILERG-ILLKMRFELDLYVNLRPVKLYHPRLTPL 115 Query: 123 REQEKGIDYVVVRENIEGLYASRTGGCN--VRDEIATDTIIITRTGTQKIVDYXXXXXXX 180 + + K ID V VREN EGLYA G EIA +I TR G ++++ + Sbjct: 116 KGKNK-IDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYAKR 174 Query: 181 XXXXXXXXXSMVTCVDKANVLSSYAY--FRKIYDEVAANYPDIAKDYAYVDAMAFYQVIQ 238 VT VDKANVL+ YA+ + +++ EV+ Y D+ D+ YVDAMA + Sbjct: 175 SGR------KKVTLVDKANVLT-YAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRS 226 Query: 239 PAQYDVLVAENMFADILSDLSSATIGGLGLAPSGDIG-DRHAMFQPSHGSAPGIAGKQIA 297 P +DV+V NMF DIL+DL + +GGLG+A SG+I + MF+P HGSAP IAGK+IA Sbjct: 227 PESFDVVVTPNMFGDILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIA 286 Query: 298 NPIATILSGGMMLDWLGEQHQDNALIQAGKDIEAAVAAVLKEGTIRTGDIGGKSSTSEVG 357 NP+A ILS MML++L L + K IE AV + E + T D+ G T EVG Sbjct: 287 NPLAAILSASMMLEYLN-------LEKESKWIEEAVKRAIAENKV-TPDMNGNLKTYEVG 338 Query: 358 DAVVQAL 364 D VV+ L Sbjct: 339 DWVVKFL 345 >MA0201 [CE] COG0473 Isocitrate/isopropylmalate dehydrogenase Length = 375 Score = 216 bits (550), Expect = 5e-56, Method: Compositional matrix adjust. Identities = 137/390 (35%), Positives = 211/390 (54%), Gaps = 48/390 (12%) Query: 1 MSRYNISVLAGDGIGPEVTEEALKVLRAVQRNYGSXXXXXXXXQNGASSGQFLQMGKAMT 60 M++Y + VL GDGIGPE+ E KV+ A +G +GA +L+ G+ ++ Sbjct: 1 MTQYKVPVLPGDGIGPEIIAEGKKVIDAAGERFG-FDVEWIEYPHGAD--HYLKTGELIS 57 Query: 61 EEQFGQAKAADAMLMGAIGLFEARHPDGREVNGDVIFRLRFDLDLYAGVRPVKLYEGVPG 120 EE + A+ +G+IG + R G G ++ RF D Y +RP+KL EGV Sbjct: 58 EETLKELSGYPAIYLGSIG--DPRIAPGVLEKG-ILLTARFYFDQYINLRPIKLLEGVWT 114 Query: 121 PLREQE-KGIDYVVVRENIEGLYASRTG----------------------GCNVR---DE 154 P++++ K ID+VVVREN E Y G G ++ +E Sbjct: 115 PIKDKTAKDIDFVVVRENTEDFYIGVGGRAKKGESKDLLEVKRTLYSAKFGLDIETDSEE 174 Query: 155 IATDTIIITRTGTQKIVDYXXXXXXXXXXXXXXXXSMVTCVDKANVLSS-YAYFRKIYDE 213 I +I++ GT+++++Y V+ VDKANVLS Y ++R+ ++ Sbjct: 175 IGYQIGLISKEGTKRVIEYAFDLAENRK-------KHVSSVDKANVLSDIYGFWREEFNA 227 Query: 214 VAANYPDIAKDYAYVDAMAFYQVIQPAQYDVLVAENMFADILSDLSSATIGGLGLAPSGD 273 VAA +PDI D+ +VDA+ + V P +DV+V NMF DI++DL + GGLGLAP G+ Sbjct: 228 VAAAHPDITTDFNFVDAITMWFVKNPEWFDVVVTPNMFGDIITDLGAMIQGGLGLAPGGN 287 Query: 274 IGDR-HAMFQPSHGSAPGIAGKQIANPIATILSGGMMLDWLGEQHQDNALIQAGKDIEAA 332 I + +MF+P HGSAP G+ NPIATI +G M+++ LGE+ +A I A Sbjct: 288 INPKGTSMFEPIHGSAPKYKGQNKVNPIATIWAGAMLIEQLGEK-------EAADTIVNA 340 Query: 333 VAAVLKEGTIRTGDIGGKSSTSEVGDAVVQ 362 + + EG ++T D+GGK++TS+VGD + + Sbjct: 341 IQKNILEGKVKTYDMGGKNTTSDVGDDIAR 370 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.316 0.134 0.379 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 26,155,035 Number of extensions: 1035909 Number of successful extensions: 3179 Number of sequences better than 1.0e-05: 55 Number of HSP's gapped: 2951 Number of HSP's successfully gapped: 61 Length of query: 379 Length of database: 38,378,431 Length adjustment: 108 Effective length of query: 271 Effective length of database: 25,922,359 Effective search space: 7024959289 Effective search space used: 7024959289 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 117 (49.7 bits)