BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0598.0019.p (240 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SMb20137 [R] COG2159 Predicted metal-dependent hydrolase of the ... 84 3e-16 SSO1189 [R] COG2159 Predicted metal-dependent hydrolase of the T... 78 1e-14 SSO2112 [R] COG2159 Predicted metal-dependent hydrolase of the T... 77 3e-14 SMb20138 [R] COG2159 Predicted metal-dependent hydrolase of the ... 70 4e-12 AGc2623 [R] COG2159 Predicted metal-dependent hydrolase of the T... 61 1e-09 >SMb20137 [R] COG2159 Predicted metal-dependent hydrolase of the TIM-barrel fold Length = 313 Score = 83.6 bits (205), Expect = 3e-16, Method: Compositional matrix adjust. Identities = 60/198 (30%), Positives = 92/198 (46%), Gaps = 17/198 (8%) Query: 48 IGDMMTKYPQRIYGMTSLDPWLAEDIYVAEVERCIKELGFVAIKLHPLGHNISP---LSK 104 I ++ KYP R G + +P + AE+E +KE GF +KLH H P L Sbjct: 78 IAELCQKYPDRFIGNFNYNPRWGPENGAAELEFHVKEYGFKMLKLHANMHGYRPDRALDW 137 Query: 105 LCNKIYELAELHQVPVLVHTGTGNPFSLPSLMIDPAKRYPGVSFILCHAGFAV---YADE 161 L + + AEL V VL+HTG G P+++P++ + +P V+FI+ H G Y+ E Sbjct: 138 LRPAMKKCAELGVV-VLIHTGDG-PYTIPTMFYPIIREFPMVNFIIGHFGIQTGGNYSFE 195 Query: 162 AIVAAKHCDNIYLEPSWCQTYMTRKMIDQVGLEKIIMGSDHISNLPVELAKY-------- 213 + A N+Y E WC + ++ KI+ G+D N P + Sbjct: 196 SFWMAMDTPNVYCESGWCFQSRIVEFAKELPKHKIVFGTDSPPNEPGMWLRELEVLCHEP 255 Query: 214 -NNIGFSDEQLEAIFYNN 230 + +G S+E LE NN Sbjct: 256 PHGLGLSEEDLEGYLGNN 273 >SSO1189 [R] COG2159 Predicted metal-dependent hydrolase of the TIM-barrel fold Length = 270 Score = 77.8 bits (190), Expect = 1e-14, Method: Compositional matrix adjust. Identities = 59/177 (33%), Positives = 89/177 (50%), Gaps = 17/177 (9%) Query: 77 EVERCIKELGFVAIKLHPLGHNISPLS--------KLCNKIYELAELHQVPVLVHTGTG- 127 E+ER LG + IKLHP+ H P + K IYE AE H +P+L+HTGT Sbjct: 94 ELERQY-SLGIIGIKLHPVHHTFKPNAYREEEGGLKQLLYIYEFAEDHGLPILIHTGTSI 152 Query: 128 -----NPFSLPSLMIDPAKRYPGVSFILCHAGFAVYADEAIVAAKHCDNIYLEPSWCQTY 182 N ++ P D AK +P + IL HAG ++ + A A+ NI+LE S Sbjct: 153 GVGSRNKYADPIYADDIAKDFPKLRIILAHAGRPLWYNTAFHMARFTQNIFLEISSIPPK 212 Query: 183 MTRKMIDQVG--LEKIIMGSDHISNLPVELAKYNNIGFSDEQLEAIFYNNPKRIFKL 237 K++ ++ +K+I GSD + +LA+Y ++ + E I +N KRI K+ Sbjct: 213 NILKVLPRIHEISDKVIYGSDFPAFKGQDLAEYAYQVYNVLKNEKIMRDNAKRILKI 269 >SSO2112 [R] COG2159 Predicted metal-dependent hydrolase of the TIM-barrel fold Length = 269 Score = 76.6 bits (187), Expect = 3e-14, Method: Compositional matrix adjust. Identities = 63/236 (26%), Positives = 110/236 (46%), Gaps = 21/236 (8%) Query: 16 EDLMQAMDRFGIAAGLVMPQPTLDNVFEVHSRIGDMMTKYPQRIYGMTSLDPWLAEDIYV 75 ++ + + FGI +V+P +D+ + RI + + RI S+DP ++ Sbjct: 38 KETLDYYESFGIKRFVVLP---IDSTTFLGRRIPNQVINLDDRIVKFISVDP--SKPNAD 92 Query: 76 AEVERCIKELGFVAIKLHPLGHNISPLSKLCNKIYELAELHQVPVLVHTGTGN------- 128 E++R IKE V +KLHP PL + KIYE+ + H + V+ HTGT Sbjct: 93 EELKRSIKEFEPVGVKLHPQLQGFHPLDERALKIYEIIDNHGLIVVFHTGTSGIGAGVKS 152 Query: 129 ----PFSLPSLMIDPAKRYPGVSFILCHAGFAVYADEAIVAAKHCDNIYLEPS-WCQTYM 183 + P + A R+ + IL H G+ + +EAI A H N+YL+ S W Y+ Sbjct: 153 SIRLDYGRPIYFDEIAVRFQNMKIILAHFGWP-WTEEAIAIALHKPNVYLDLSGWAPRYI 211 Query: 184 TRKMIDQVGL--EKIIMGSDHISNLPVE-LAKYNNIGFSDEQLEAIFYNNPKRIFK 236 + + +K++ GSD P L ++ ++ S + + I +N +++ K Sbjct: 212 PQVIWSNAKRLSDKLLFGSDFPLIRPERWLEEFKSVNLSQDIKDKILKHNAEKLVK 267 >SMb20138 [R] COG2159 Predicted metal-dependent hydrolase of the TIM-barrel fold Length = 308 Score = 69.7 bits (169), Expect = 4e-12, Method: Compositional matrix adjust. Identities = 56/204 (27%), Positives = 86/204 (42%), Gaps = 17/204 (8%) Query: 50 DMMTKYPQRIYGMTSLDPWLAEDIYVAEVERCIKELGFVAIKLHPLGHNISP---LSKLC 106 +++ KYP R G +P E+E KE GF ++L H P L L Sbjct: 82 ELVQKYPDRFLGCFVYNPRYGTQNGAEEIEHHAKEYGFKMVQLQANMHAYRPDRALDWLR 141 Query: 107 NKIYELAELHQVPVLVHTGTGNPFSLPSLMIDPAKRYPGVSFILCHAGF---AVYADEAI 163 + + A+L + V VHTG G P+S+P+ + +P V+F+L H G VY E Sbjct: 142 PAMRKCADLGLI-VKVHTGDG-PYSIPTEFYPIIREFPSVNFVLGHFGVQTGGVYVFEPF 199 Query: 164 VAAKHCDNIYLEPSWCQTYMTRKMIDQVGLEKIIMGSDHISNLPVELAKY---------N 214 A NIY+E WC + ++ KI+ G+D N P + Sbjct: 200 QMAMDAPNIYVESGWCLQSRIVEFAKELPKHKILFGTDTPPNEPGMWLRLLEVLCHEPPQ 259 Query: 215 NIGFSDEQLEAIFYNNPKRIFKLE 238 + ++ LE NN R+ L+ Sbjct: 260 GLNLDEDTLEDYLGNNIARMIGLD 283 >AGc2623 [R] COG2159 Predicted metal-dependent hydrolase of the TIM-barrel fold Length = 291 Score = 61.2 bits (147), Expect = 1e-09, Method: Compositional matrix adjust. Identities = 48/179 (26%), Positives = 72/179 (40%), Gaps = 19/179 (10%) Query: 77 EVERCIKELGFVAIKLHPLGHNISPLSKLCNKIYELAELHQVPVLVHTGTGN-------- 128 E R ++E G K HP P ++ +YE L HTG Sbjct: 114 EARRLVREFGVKGFKFHPTMQGFYPNDRMAYPLYEAIAEEGAITLFHTGQTGVGAGLRGG 173 Query: 129 -----PFSLPSLMIDPAKRYPGVSFILCHAGFAVYADEAIVAAKHCDNIYLEPS-WCQTY 182 FS P + D A +P + IL H F + +EA+ A H N+Y++ S W Y Sbjct: 174 MAMRLKFSNPIHLDDVAVDFPDMPIILAHPSFP-WQEEALSVATHKPNVYIDMSGWSPKY 232 Query: 183 MTRKMI---DQVGLEKIIMGSDHISNLPVE-LAKYNNIGFSDEQLEAIFYNNPKRIFKL 237 ++ + + K++ GSD + P L + NI DE I N +R+ KL Sbjct: 233 FPPILVQYANSLLKHKMLFGSDWPAMTPERWLNDFANISIKDEVRPLILKENARRLLKL 291 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.322 0.138 0.420 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 18,795,420 Number of extensions: 781846 Number of successful extensions: 1911 Number of sequences better than 1.0e-05: 11 Number of HSP's gapped: 1906 Number of HSP's successfully gapped: 11 Length of query: 240 Length of database: 38,378,431 Length adjustment: 103 Effective length of query: 137 Effective length of database: 26,499,029 Effective search space: 3630366973 Effective search space used: 3630366973 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 114 (48.5 bits)