BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0598.0006.p (662 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CAC0706 [G] COG2730 Endoglucanase 53 1e-06 >CAC0706 [G] COG2730 Endoglucanase Length = 722 Score = 53.1 bits (126), Expect = 1e-06, Method: Compositional matrix adjust. Identities = 64/276 (23%), Positives = 124/276 (44%), Gaps = 23/276 (8%) Query: 385 MLVNKNGKAMDLIGGNQNNGAVINQWAYDYNGPNQRWSVIPAGNGHFRIASSVSGKYAAV 444 ++ + GK +++ + +GA I + D +++W + +GNG ++I S++S ++ Sbjct: 423 IISSSTGKVLNVRNYSSQSGAQIEELV-DVGALSEQWKIADSGNGSYKITSALSN--LSL 479 Query: 445 QGNSTSDGAQLVSAPYEEGNAFQQWDFVDAGNGWFKIVNVGSGKVLDVDKNSLANEAKMQ 504 G +++G + Y G QQW+ +KI N S L ++ + + A +Q Sbjct: 480 DGGVSTEGTDVKQYQYA-GAENQQWNIEKINANTYKITNKKSN--LALETTGVYDSADIQ 536 Query: 505 QWAWTGAECQYWRIQPQGDYYIQAEHSGKYLNAGGAGTANETPVIQFTFENNIWFKWRFE 564 W +TG Q W I +NAG T T N Sbjct: 537 LWNYTGKGTQQWIISD--------------VNAGTNTTPTTGGNTTPTTGGNTTPTTGSA 582 Query: 565 STGEGYYKVLNLNASNKVADVSNVSTADGAKVHLWDDVNAGNQQVRLVPQQDGSFKLFFK 624 YK+++ N + KV +V N S+ GA++ DV A +++ ++ +GS+K+ Sbjct: 583 IDNSKTYKIISQN-TGKVLNVRNYSSESGAQIEELVDVGALSEEWKITNSGNGSYKITSA 641 Query: 625 HSNKAWDIEEFSRNNEARLTQYTEVTADNQKFYLER 660 SN + D + ++ + QYT A++Q++ +E+ Sbjct: 642 LSNFSLDAGTSTEGSDVKQYQYT--GAESQQWNIEK 675 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.314 0.131 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 53,511,223 Number of extensions: 2402669 Number of successful extensions: 3132 Number of sequences better than 1.0e-05: 4 Number of HSP's gapped: 3115 Number of HSP's successfully gapped: 5 Length of query: 662 Length of database: 38,378,431 Length adjustment: 113 Effective length of query: 549 Effective length of database: 25,345,689 Effective search space: 13914783261 Effective search space used: 13914783261 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 119 (50.4 bits)