BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0596.0027.p (516 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH1910 [T] COG4753 Response regulator containing CheY-like recei... 267 3e-71 BH0793 [T] COG4753 Response regulator containing CheY-like recei... 159 1e-38 BH2109 [T] COG4753 Response regulator containing CheY-like recei... 122 1e-27 BH1123 [T] COG4753 Response regulator containing CheY-like recei... 107 4e-23 BH2728 [T] COG4753 Response regulator containing CheY-like recei... 102 1e-21 >BH1910 [T] COG4753 Response regulator containing CheY-like receiver domain and AraC-type DNA-binding domain Length = 506 Score = 267 bits (683), Expect = 3e-71, Method: Compositional matrix adjust. Identities = 175/522 (33%), Positives = 267/522 (51%), Gaps = 35/522 (6%) Query: 1 MIKVVIVDDEPKLRQGLQTLIPWENLGFTVTAAAGNGRDALKIVEEEVPDLVIADIRMPV 60 M V+IVDDE +R+GL+ LI WE GF V A NG++ + PDL+I DIRMP Sbjct: 1 MYDVMIVDDEAMVREGLKALISWEEYGFRVIDTARNGKEGFTKYRQLSPDLMIVDIRMPE 60 Query: 61 MDGLQFIQRLRSAGHQMHCIILSGYADFEYAKQAITYGVAGYLVKPVNIAEMSATLKLVX 120 +DGL I+ +R+ +H IILSG++DF YAK+A+ YL+KP++ E+ +LK V Sbjct: 61 VDGLSLIESIRAEDSAIHFIILSGHSDFNYAKKALGCQCDDYLLKPLDEDELIDSLKKVY 120 Query: 121 XXXXXXXXXXXWY-RTEVTNRELYLRRLLAPQEKTEDPAKLRSRIREMDLLWSHYEIVVI 179 R + E+ L LL +E P +E + + Y++ ++ Sbjct: 121 GRITRHHQVHELVERVQEKQVEMVLTELLEGTPLSEIPIG-----KEHNFFATDYQVALV 175 Query: 180 SPRVPEADRPEHLHQLSAGLKHGIEGRNRGLVTVIAPYVILLLNSPLHGRQRRDHLYGEI 239 R DR L+ LK IE + G V Y++++ + ++ +DH Y Sbjct: 176 ENR---QDR------LTKQLKRLIEEQEEGYVIPFPEYIVIVCKNRFLQKRGKDHRYHLF 226 Query: 240 RSIAGDTRFIAATGGTVSEPELIYSAFLKAQEAVKQVFFSAKDCLLEPE----RTLFAAP 295 + A G V IY ++ A+ ++ FF K L + R+ AP Sbjct: 227 AKWLEEEELHACAGSKVVNLADIYESYQSAKRLYEKKFFVEKGRLFTADQLVDRSKPEAP 286 Query: 296 EFPREAGDRDEKRMEDLIFRLYYSLDAGNKTMVLPLLNEAAAFFIQQGQEEDAVKQAFFF 355 EF +E RLYYS+D GN + ++ E I QEE +K A F Sbjct: 287 EFS----------LESYADRLYYSIDIGNMEGCVAIIEELKNVVIVHLQEEKEIKVALFQ 336 Query: 356 LSNRLIHKLTAASRIELKETETVSRFLNGIYQQDYLSDL---LEETHRFLLEFAEDAGPK 412 L + ++H+L + E + IYQ ++L + L + +++++ ++ P Sbjct: 337 LYSNILHQLVRSHPNMEGVLEPLVMNAGDIYQFEHLPGIMAFLTKQLKYVIDCMDNGQP- 395 Query: 413 GKEQEIKQMTGFIHRHYAEDLKLSTLAGLLNYSTPYLGQLFRNKTGEYFNTYLDKVRIQK 472 + +++M IH+ YA++L+L TLA + NY+ YLG+LF+N TGEYFNTYLDKVRI+ Sbjct: 396 --DLLLRKMIALIHKDYAKNLRLETLASVFNYNRAYLGKLFKNYTGEYFNTYLDKVRIEN 453 Query: 473 AKELLAQGMKVYEAAELVGYTSVNYFFSKFKKYEGRSPSDYK 514 K LL +G KVYE AE +GY +V+YF KFKKY G SPS ++ Sbjct: 454 GKRLLQKGYKVYEVAEKIGYANVDYFHRKFKKYVGVSPSTFR 495 >BH0793 [T] COG4753 Response regulator containing CheY-like receiver domain and AraC-type DNA-binding domain Length = 508 Score = 159 bits (401), Expect = 1e-38, Method: Compositional matrix adjust. Identities = 145/547 (26%), Positives = 242/547 (44%), Gaps = 76/547 (13%) Query: 1 MIKVVIVDDEPKLRQGLQTLIPWENLGFTVTAAAGNGRDALKIVEEEVPDLVIADIRMPV 60 MI+V++VDDEP + +GL+ LI W LG+ + A A NG +AL+I + DL+I DI+MP Sbjct: 1 MIRVMLVDDEPLILEGLKHLIDWGQLGYEIVATARNGVEALEIAKYVSFDLLITDIKMPE 60 Query: 61 MDGLQFIQRLRSAGHQMHCIILSGYADFEYAKQAITYGVAGYLVKPVNIAEMSATLKLVX 120 GL+ I L + I+LSG+ +F + KQ +T G+ YL+KP+N E+ +TL V Sbjct: 61 KTGLELIAALNEREKTIRSIVLSGFQEFHFVKQGLTLGIENYLLKPINEKELISTLSHVK 120 Query: 121 XXXXXX------------XXXXXWYRTEVTNRELYLRRLLAPQEKTEDPAKLRSRIREMD 168 W ++ + E R P+ + + P RI + Sbjct: 121 EKINHAFLKEESTLVLRDHSIWRWLVGKMGHTEFKQRMAFYPEVQWKFPL----RIGLLK 176 Query: 169 LLWSHYEIVVISPRVPEADRPEHLHQLSAGLKHGIEGRNRGLVTVIAPYVILLLNSPLHG 228 + W + E L++L L+ + +TV+ P LL Sbjct: 177 MEWDQH-------------TEEELYRLQRTLE-----QETSAMTVVTPSGDLLFIWCGFE 218 Query: 229 R-----QRRDHLYGEIRSIAGDTRFIAATGGTVSEPELIYSAFLKAQEAVKQVFFSAKDC 283 R Q R + F+ +SE + +Y E +++ + Sbjct: 219 RESDWGQERSRFQSVVTEAPIGQPFVFVHSEPISEIQCLY-------EVYREMEMKCELK 271 Query: 284 LLEPERTLFAAPE--FPREAG--------DRDEKRMEDLIFRLYYSLDAGNKTMVLPLLN 333 LL PER A + F RE+ ++ E L R Y + + M+ Sbjct: 272 LLLPERDHHMADQLTFKRESELIPPQMNITLEQDIFEHLANRHYQKVRKDLEHMLSESHC 331 Query: 334 EAAAFFIQQGQEEDAVKQAFFFLSNRLIHKLTAASRIELKE----TETVSRFLNGIYQQD 389 E F++ + + F+ L ++ I +L +++ + ET S + I D Sbjct: 332 ERNPIFLK-----SVLMEFFYRLKSQFIVQLPFDQYVQIVQQIVHVETTSEAMKMI---D 383 Query: 390 YLSDLLEETHRFLLEFAEDAGPKGKEQEIKQMTGFIHRHYAEDLKLSTLAGLLNYSTPYL 449 DLLE+ H L E ++ P I+ + IH+H+++DL L TL + + YL Sbjct: 384 RCIDLLEDDH--LQENEKNYSPI-----IQTVLQHIHQHFSQDLSLKTLGHSFHINPIYL 436 Query: 450 GQLFRNKTGEYFNTYLDKVRIQKAKELLAQGM-KVYEAAELVGYTSVNYFFSKFKKYEGR 508 GQLF+ + G F YL+++RI +AK+LL + + + VGY YF+ +FKKYE Sbjct: 437 GQLFQREVGCSFTKYLNQLRIDRAKKLLLNSYDRAGQIGKKVGYGDATYFYKQFKKYEAM 496 Query: 509 SPSDYKN 515 +P++++ Sbjct: 497 TPTEWRK 503 >BH2109 [T] COG4753 Response regulator containing CheY-like receiver domain and AraC-type DNA-binding domain Length = 525 Score = 122 bits (307), Expect = 1e-27, Method: Compositional matrix adjust. Identities = 57/117 (48%), Positives = 79/117 (67%), Gaps = 1/117 (0%) Query: 1 MIKVVIVDDEPKLRQGLQTLIPWENLGFTVTAAAGNGRDALKIVEEEVPDLVIADIRMPV 60 M KV++ DDE +R GL++LI WE G+ V A A NG DA K+V+E PDLVI DIRMPV Sbjct: 1 MYKVLLADDEFFVRHGLKSLIEWEKCGYEVAAEADNGEDAFKLVQEIKPDLVITDIRMPV 60 Query: 61 MDGLQFIQRLR-SAGHQMHCIILSGYADFEYAKQAITYGVAGYLVKPVNIAEMSATL 116 DGLQ IQ++ + II+SGY DF YA++A+ +G +++KP++ E+ A L Sbjct: 61 FDGLQLIQKVNETLSFNTKFIIISGYNDFSYAQKAVRFGAVDFVLKPIDQDELEAAL 117 Score = 86.7 bits (213), Expect = 9e-17, Method: Compositional matrix adjust. Identities = 45/120 (37%), Positives = 76/120 (63%), Gaps = 4/120 (3%) Query: 400 RFLLEFA---EDAGPKGKEQEIKQMTGFIHRHYAEDLKLSTLAGLLNYSTPYLGQLFRNK 456 +F+LE A ++ + + +I ++ +I HY E++ L ++A L + Y+GQLF+ Sbjct: 402 KFVLEAAVIIQELRKENMKSDIYKVKTYIENHYHENISLKSIASLFYMNPVYMGQLFKKT 461 Query: 457 TGEYFNTYLDKVRIQKAKELLAQ-GMKVYEAAELVGYTSVNYFFSKFKKYEGRSPSDYKN 515 G YF YL K+R+ +AK+LL Q M+VYE AE VG+ S +YF ++F+K +P++Y+N Sbjct: 462 FGVYFKEYLLKLRMNEAKKLLRQTDMRVYEVAERVGFGSTDYFVTQFEKINKMTPTEYRN 521 >BH1123 [T] COG4753 Response regulator containing CheY-like receiver domain and AraC-type DNA-binding domain Length = 526 Score = 107 bits (267), Expect = 4e-23, Method: Compositional matrix adjust. Identities = 52/119 (43%), Positives = 72/119 (60%) Query: 1 MIKVVIVDDEPKLRQGLQTLIPWENLGFTVTAAAGNGRDALKIVEEEVPDLVIADIRMPV 60 M KVVI+DDE + +GL+ +I W+ LG A NGR L IV E P +V+ DI MP+ Sbjct: 1 MWKVVIIDDEEHVVRGLKKIISWKALGCECVGEAANGRRGLDIVRETSPHIVLTDIYMPI 60 Query: 61 MDGLQFIQRLRSAGHQMHCIILSGYADFEYAKQAITYGVAGYLVKPVNIAEMSATLKLV 119 M GL IQ LR G +ILSGY++FE+A++A+ + YL KP ++ + LK V Sbjct: 61 MYGLDMIQILREDGFDGEVVILSGYSEFEHARKAMQLNITDYLSKPASVDTIETVLKRV 119 Score = 83.6 bits (205), Expect = 8e-16, Method: Compositional matrix adjust. Identities = 46/126 (36%), Positives = 73/126 (57%), Gaps = 2/126 (1%) Query: 388 QDYLSDLLEETHRFLLEFAEDAGPKGKEQEIKQMTGFIHRHYAEDLKLSTLAGLLNYSTP 447 Q+++ L E+ D K K Q I+Q+ +I +H AE++ L +A L S Sbjct: 397 QEWIRFLCEQIEHICYHQQWDENIKHK-QLIEQVMIYIEKHLAENITLQDIADELYISRN 455 Query: 448 YLGQLFRNKTGEYFNTYLDKVRIQKAKELLAQG-MKVYEAAELVGYTSVNYFFSKFKKYE 506 YLGQ+F+ GE F Y+ ++R++KAK+++ +G +YE +E VGY + YF + FKKY Sbjct: 456 YLGQIFKKVVGESFKNYVTRLRMEKAKKMIQEGNFLIYEVSEKVGYVNPAYFTTMFKKYT 515 Query: 507 GRSPSD 512 G +P D Sbjct: 516 GYTPRD 521 >BH2728 [T] COG4753 Response regulator containing CheY-like receiver domain and AraC-type DNA-binding domain Length = 510 Score = 102 bits (254), Expect = 1e-21, Method: Compositional matrix adjust. Identities = 47/120 (39%), Positives = 74/120 (61%), Gaps = 1/120 (0%) Query: 1 MIKVVIVDDEPKLRQGLQTLIPWENLGFTVTAAAGNGRDALKIVEEEVPDLVIADIRMPV 60 M V+IVDDE +RQG+ WE GF + A NG+DAL+++ + P +V+ DI MPV Sbjct: 1 MCNVLIVDDEKLIRQGIINFFDWEKEGFQIVGEAANGKDALELISKNEPHIVMTDIVMPV 60 Query: 61 MDGLQFIQRLRSAGHQMHCIILSGYADFEYAKQAITYGVAGYLVKP-VNIAEMSATLKLV 119 MDG+ ++R++ ++ I+LS + +FEY + GVA Y++KP +N ++ TL V Sbjct: 61 MDGIDLVKRVKRDFPEVEIIVLSSFEEFEYVRSTFHSGVADYILKPRLNEKDLLKTLSEV 120 Score = 75.5 bits (184), Expect = 2e-13, Method: Compositional matrix adjust. Identities = 41/98 (41%), Positives = 58/98 (59%), Gaps = 1/98 (1%) Query: 418 IKQMTGFIHRHYAEDLKLSTLAGLLNYSTPYLGQLFRNKTGEYFNTYLDKVRIQKAKELL 477 I++M +I H++E L LS LA +++ YL FR E F+ YL+KVRI+KAKE L Sbjct: 402 IQKMLAYIEEHHSEPLSLSQLANYFHFNPSYLSAYFRQHHDEGFSEYLNKVRIRKAKEWL 461 Query: 478 -AQGMKVYEAAELVGYTSVNYFFSKFKKYEGRSPSDYK 514 + + + E LVGY+ +YF FKK G PS Y+ Sbjct: 462 KSSTIPISEIYALVGYSDHSYFCKVFKKRTGMPPSRYR 499 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.137 0.395 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 36,716,989 Number of extensions: 1576685 Number of successful extensions: 6690 Number of sequences better than 1.0e-05: 331 Number of HSP's gapped: 6739 Number of HSP's successfully gapped: 342 Length of query: 516 Length of database: 38,378,431 Length adjustment: 111 Effective length of query: 405 Effective length of database: 25,576,357 Effective search space: 10358424585 Effective search space used: 10358424585 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 118 (50.1 bits)