BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0596.0012.p (1944 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CAC0425 [G] COG1621 Beta-fructosidases (levanase/invertase) 187 2e-46 BS_sacC [G] COG1621 Beta-fructosidases (levanase/invertase) 184 1e-45 STM1414 [U] COG4789 Type III secretory pathway, component EscV 149 4e-35 CAC1462 [G] COG1621 Beta-fructosidases (levanase/invertase) 122 6e-27 BS_yveB [G] COG1621 Beta-fructosidases (levanase/invertase) 112 9e-24 >CAC0425 [G] COG1621 Beta-fructosidases (levanase/invertase) Length = 346 Score = 187 bits (474), Expect = 2e-46, Method: Composition-based stats. Identities = 0/466 (0%), Positives = 0/466 (0%), Gaps = 28/466 (6%) Query: 842 DQHRPQYHAMPPQNWMNEAHAPIYYNGKYHLFYQHNPQGPYWHQIHWGHWVSDDMVHWEN 901 + + A ++ + + + Sbjct: 24 GEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLI 83 Query: 902 VRPALAPEAGTLDPDGVWSGSAAYDRDGNPVLFYTAGNDSLSPNQRTGLATPADLSDPNL 961 + ++ + G+ A DR + L + Sbjct: 84 IASKVSG-PSRNNDKGIRPDQAL-DRKNIREALHD-SLKRLQTDYLDLYQVHWPQRP-TN 139 Query: 962 EKWVKYPEPVTEQNGNGIHNEFRDPFVWYDKETDKWYQLVTSGLKDFSSGTALVYVSDDM 1021 + + + + K + + ++++D Sbjct: 140 CFGKLGYSWTDSAPAVSLLDTLDALAEY--QRAGKIR-YIGVSNETAFGVMRYLHLADKH 196 Query: 1022 YNWEYKGPLYVSDRSLYPELGTVWELPVLLPLGKDSTGKQKYIFMINPHEKPEQVPPAND 1081 + E+ + + + + Sbjct: 197 DLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGV------ELLAYSCLGFGTLTGKYLNGAK 250 Query: 1082 VLRDVEVFYWIGTWDRDNFKFIPDQDAPSKMDVGDGY-LTAESGMVTPDGRTVVYS-MVQ 1139 + + + V V Sbjct: 251 PA---GARNTLFSRFTRYSGE----QTQKAVAAYVDIARRHGLDPAQMALAFVRRQPFVA 303 Query: 1140 NVRTPQAEYQAGWAHNLALPVSLSLDEHDELRIEPIQELQSLRGAKLVDFADKNLQAANQ 1199 + + +L + LS D E+ Sbjct: 304 STLLGATTMDQLKTNIESLHLELSEDVLAEIE-- 361 Query: 1200 LIQNVKGDMLEIVMEIDPGQAQKFGLKVRRSDNGQEETLIYYDKTAGTFNVDRTKSSIDP 1259 Sbjct: 362 421 Query: 1260 DVRVDGIQGGYVDLKGENLKLHIFLDRSVVEAFAND-KKKLTTRVY 1304 Sbjct: 422 --- 464 >BS_sacC [G] COG1621 Beta-fructosidases (levanase/invertase) Length = 346 Score = 184 bits (467), Expect = 1e-45, Method: Compositional matrix adjust. Identities = 0/526 (0%), Positives = 0/526 (0%), Gaps = 61/526 (11%) Query: 835 DPSVFDGDQHRPQYHAMPPQNWMNEAHAPIYYNGKYHLFYQHNPQGPYWHQIHWGHWVSD 894 S D + Y N ++ A G + + + Sbjct: 26 QNSEADAH-AQLDYAVAQGINLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLII 84 Query: 895 DMVHWENVRPALAP-EAGTLDPDGVWSGSAAYDRDGNPVLFYTAGNDSL----SPNQRTG 949 R + + L + QR Sbjct: 85 ASKVSGPSRNNDKGIRPDQ-----ALDRKNIREAL-HDSLKRLQTDYLDLYQVHWPQRPT 138 Query: 950 LATPADLSDPNLEKWVKYP-EPVTEQNGNGIHNEFRDPFV-WYDKETDKWYQLVTSGLKD 1007 + + ET + + L Sbjct: 139 NCFGKLGYSWTDSAPAVSLLDTLDALAEY---QRAGKIRYIGVSNET-AFGVMRYLHLA- 193 Query: 1008 FSSGTALVYVSDDMYNWEYKGPLYVSDRSLYPELGTVWELPVLLPLGKDSTGKQKYIFMI 1067 + + + + L S + Sbjct: 194 -----DKHDLPRIVTIQNPYSL-LNRSFEVG---LAEVSQYEGVELLAYSCLGFGTLTG- 243 Query: 1068 NPHEKPEQVPPANDVLRDVE-VFYWIGTWDRDNFKFIPDQDAPSKM---DVGDGYLTAES 1123 + +F + + + A G Sbjct: 244 -------KYLNGAKPAGARNTLFSRFTRYSGEQTQ---KAVAAYVDIARRHGLDPAQMAL 293 Query: 1124 GMVTP--DGRTVVYSMVQNVRTPQAEYQAGWAHNLALPVSLSLDEHDE-LRI--EPIQEL 1178 V + + + + + + Sbjct: 294 AFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEIEAVHQVYTYPA 353 Query: 1179 QSLRGAKLVDFADKNLQ---AANQLIQNVKGDMLEIVME--IDPGQAQKFGLKVRRSDNG 1233 Sbjct: 354 ---- 409 Query: 1234 QEETLIYYDKTAGTFNVDRTKSSIDPDVRVDGIQGGYVDLKGEN--LKLHIFLDRSVVEA 1291 Sbjct: 410 - 468 Query: 1292 FANDKKKLTTRVYVGRYDSLGLQVWADN-DITVKSMEVWNMNALTG 1336 Sbjct: 469 514 >STM1414 [U] COG4789 Type III secretory pathway, component EscV Length = 681 Score = 149 bits (376), Expect = 4e-35, Method: Compositional matrix adjust. Identities = 29/464 (6%), Positives = 76/464 (16%), Gaps = 65/464 (14%) Query: 845 RPQYHAMPPQNWMNEAHAPIYYNGKYHLFYQHNPQGPYWHQIHWGHWVSDDMVHWENVRP 904 + Q W++ L ++ +++ + Sbjct: 3 SWLGEGVRAQQWLSVCAGRQDMVLATVLLIAIVMMLLPLPTWMVDILITINLMFSVILLL 62 Query: 905 ALAPEAGTLDPDGVWSGSAAYDRDGNPVLFYTAGNDSLSPNQRTGLATPADLSDPNLEKW 964 + + + + S A + Sbjct: 63 IAIYLSDP---LDLSVFPSL-LLITTLYRLSLTISTS-RLVLLQHNAGNIVDAFGKFV-V 116 Query: 965 VKYP-EPVTEQNGNGIHNEFRDPFVWYDKETDKWYQLVTSGLKDFSSGTALVYVSDDMYN 1023 + I + + + ++ Sbjct: 117 GGNLTVGLVVFTIITIVQFIVITKG--IERVAEVSARFSLD-GMPGKQMSIDGDLRAGVI 173 Query: 1024 WEYKGPLYVSDRSLYPELGTVWELPVLLPLGKDSTGKQKYIFMINPHEKPEQVPPANDVL 1083 E + K I VL Sbjct: 174 DADHARTLRQHVQ--QES----RFLGAMDGAM----KFVKGDTIA---------GIIVVL 214 Query: 1084 RDVEVFYWIGTWDRDNFKFIPDQDAPSKMDVGDGYLTAESGMVTPDGRTVVYSMVQN-VR 1142 I Q S + Y G + ++ Sbjct: 215 ------VNIIGGIIIAI----VQYDMSMSEAVHTYSVLSIGDGL--CGQIPSLLISLSAG 262 Query: 1143 T-PQAEYQAGWAHNLALPVSLSLDEHDELRIEPIQELQSLRGAKLVDFADKNLQAANQLI 1201 + S + I L L F Sbjct: 263 IIVTRVPGEKRQNLATELSSQIA-RQPQSLILTAVVLMLLALIPGFPFI-----TLAFFS 316 Query: 1202 QNVKGDMLEIVMEIDPGQAQKFGLKVRRSDNGQEETLIYYDKTAGTFNVDRTKSSID-PD 1260 + ++ I + + + ++++++ Sbjct: 317 ALLALPIILIRRKKS-VVSA------NGVEAPEKDSMV--PGACPLILRLSPTLHSADLI 367 Query: 1261 VRVDGIQGGYVDLKGENLKLHIFLDRSVVEAFANDKKKLTTRVY 1304 +D ++ + + L +E +KLT +Y Sbjct: 368 RDIDAMRWFLFE------DTGVPLPEVNIEVLPEPTEKLTVLLY 405 >CAC1462 [G] COG1621 Beta-fructosidases (levanase/invertase) Length = 346 Score = 122 bits (306), Expect = 6e-27, Method: Compositional matrix adjust. Identities = 0/411 (0%), Positives = 0/411 (0%), Gaps = 44/411 (10%) Query: 1120 TAESGMVTPDGRTVVYSMVQN----VRTPQAEYQAGWAHNLALPVSLSL-DEHDELRI-- 1172 Sbjct: 586 ---- 641 Query: 1173 EPIQELQSLRGAKLVDFADKNLQAANQLIQNVKGDMLEIV--MEIDPGQAQKFGLKVRRS 1230 Sbjct: 642 - 700 Query: 1231 DNGQEETLIYYDKTAGTFNVDRTKSS----ID--PDVRVDGIQGGYVDLKGENLKLHIFL 1284 Sbjct: 701 ------ 754 Query: 1285 DRSVVEAFA----NDKKKLTTRVYVGRYDSLGLQVWADNDIT-VKSMEVWNMNALTGEPA 1339 Sbjct: 755 - 813 Query: 1340 APVDVPDNWDNSVYTDITDLPNHDFATGDLTGWITEGNAFQNVH-VTDAKFFWDTIYFNP 1398 Sbjct: 814 ------- 866 Query: 1399 SHKIPGGYHLWGFNEAAGGDSLTGTLKSQKFVLGGNGKINFLVSGGRDIDKLYVALVRAS 1458 Sbjct: 867 ---- 922 Query: 1459 DGKELFKETATNYEEYQRKIWDASQYIGQELYIKVVDQSTGGFGHINVDDF 1509 Sbjct: 923 973 Score = 121 bits (303), Expect = 1e-26, Method: Compositional matrix adjust. Identities = 0/157 (0%), Positives = 0/157 (0%), Gaps = 14/157 (8%) Query: 1358 DLPNHDFATGDLTGWITEGNAFQNVHVTDAKFFWDTIYFNPSHKIPGGYHLWGFNEAA-- 1415 Sbjct: 393 --------- 443 Query: 1416 -GGDSLTGTLKSQKFVLGGNGKINFLVSGGRDIDKLYVALVRASDGKEL--FKETATNYE 1472 Sbjct: 444 503 Query: 1473 EYQRKIWDASQYIGQELYIKVVDQSTGGFGHINVDDF 1509 Sbjct: 504 540 Score = 117 bits (294), Expect = 2e-25, Method: Compositional matrix adjust. Identities = 15/286 (5%), Positives = 36/286 (12%), Gaps = 45/286 (15%) Query: 837 SVFDGDQHRPQYHAMPPQNWMNEAHAPIYYNGKYHLFYQHNPQGPYWHQIHWGHWVSDDM 896 + + P + ++ VS Sbjct: 33 HAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPS 92 Query: 897 VHWENVRPALAPEAGTLDPDG-VWSGSAAYDRDGNP------------VLFYTAGNDSLS 943 + + + + Sbjct: 93 RNNDKGIRPDQA-----LDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCF-GKLGY 146 Query: 944 PNQRTGLATPADLSDPNLEKWVKY--PEPVTEQNGNGIHNEFRDPFVWYDKETDKWYQLV 1001 + A + + G NE + Y DK Sbjct: 147 SWTDSAPAVSLL----DTLDALAEYQRAGKIRYIGV--SNETAFGVMRYLHLADKHDLPR 200 Query: 1002 TSGLKDFSSGTALVYVSDDMYNWEYKGPLYVSDRSLYPELGTVWELPVLLPLGKDSTGKQ 1061 ++ + + + L L + K Sbjct: 201 IVTIQ------NPYSLLNRSFEVGLAEVSQYEGVELL---AYSCLGFGTLTGKYLNGAKP 251 Query: 1062 KYIFMIN--PHEKPEQVPPANDVLRDVEVFYWIGTWDRDNFKFIPD 1105 + Y R + Sbjct: 252 AGARNTLFSRFTRYSG-EQTQ----KAVAAYVDI--ARRHGLDPAQ 290 Score = 94.7 bits (234), Expect = 1e-18, Method: Compositional matrix adjust. Identities = 0/195 (0%), Positives = 0/195 (0%), Gaps = 33/195 (16%) Query: 85 IQNPSFETGDMSGWTVVRGQAFGPDSVSDETTWWAEQIPYNQEGTYHLNGWK------YG 138 Sbjct: 394 ------- 446 Query: 139 EAATGVLRSSTFELGGSGWISFKLGGAKNPNKAYVNIVEAQTGQVIARYGNSAFADVGFP 198 Sbjct: 447 ---------- 496 Query: 199 NPAQGLRLANMEQYKANLSGYLGKKLYVEIVDNATNDWGVVFADAFFMYH--------ES 250 Sbjct: 497 -- 554 Query: 251 EPAAGITATDIKPDF 265 Sbjct: 555 569 Score = 87.8 bits (216), Expect = 2e-16, Method: Compositional matrix adjust. Identities = 0/238 (0%), Positives = 0/238 (0%), Gaps = 37/238 (15%) Query: 208 NMEQYKANLSGYLGK---KLYVEIVDNATNDWGVVFADAFFMYHESEPAAGITATD-IKP 263 Sbjct: 336 ------ 389 Query: 264 DFKRYQIENPGFETGNLTGWTVVEGEAFGPNSVSDETTYWVEQIPYNQEGTYHLNGLRYN 323 Sbjct: 390 --------- 440 Query: 324 ETA------TGKLRSSTFELGGTGWITFRLGGGKHTDQVYVNVINADTGELIARYGNTEF 377 Sbjct: 441 ----- 495 Query: 378 NESGFPYPAQGLRLANMEQYKADLSKYIGKKLYVEIVDNGHADWGVIFADAFHTFNEL 435 Sbjct: 496 ------- 546 Score = 80.9 bits (198), Expect = 2e-14, Method: Compositional matrix adjust. Identities = 0/153 (0%), Positives = 0/153 (0%), Gaps = 19/153 (12%) Query: 450 EIQNPSFETGNLESWTAQGNAFQ----VNNDAQAGKEGNYYVKSSLE---------GQGS 496 Sbjct: 393 452 Query: 497 ITSNTFTLQGTGTINFTVLGINNPQDAYVALYDASTN---TLLEKTGDVSAN-EKVSWKM 552 Sbjct: 453 512 Query: 553 QKHYNKRLYIKVIDQSNQA--SISVDAFQAHGT 583 Sbjct: 513 545 Score = 67.0 bits (162), Expect = 3e-10, Method: Compositional matrix adjust. Identities = 0/164 (0%), Positives = 0/164 (0%), Gaps = 25/164 (15%) Query: 90 FETGDMSG-WTVVRGQAFGPDS---VSDETTWWAEQIPYNQEGTYHLNGW-KYGEAATGV 144 Sbjct: 834 ------ 887 Query: 145 LRSSTFELGGSGWISFKLGGAKNPNKAYVNIVEAQTGQVIARYGNSAFADVGFPNPAQGL 204 Sbjct: 888 -------------- 933 Query: 205 RLANMEQYKANLSGYLGKKLYVEIVDNATNDWGVVFADAFFMYH 248 Sbjct: 934 977 Score = 66.6 bits (161), Expect = 4e-10, Method: Compositional matrix adjust. Identities = 0/157 (0%), Positives = 0/157 (0%), Gaps = 23/157 (14%) Query: 275 FETGNLTG-WTVVEG--EAFGPNSVSDETTYWVEQIPYNQEGTYHLNGLRYN-ETATGKL 330 Sbjct: 834 ----- 888 Query: 331 RSSTFELGGTGWITFRLGGGKHTDQVYVNVINADTGELIARYGNTEFNESGFPYPAQGLR 390 Sbjct: 889 -------------- 934 Query: 391 LANMEQYKADLSKYIGKKLYVEIVDNGHADWGVIFAD 427 Sbjct: 935 971 >BS_yveB [G] COG1621 Beta-fructosidases (levanase/invertase) Length = 346 Score = 112 bits (279), Expect = 9e-24, Method: Compositional matrix adjust. Identities = 0/515 (0%), Positives = 0/515 (0%), Gaps = 76/515 (14%) Query: 844 HRPQYHAMPPQNWMNEAHAPIYYNGKYHLFYQHNPQGPYWHQIHWGHWVSDDMVHWENVR 903 + + S + Sbjct: 43 GINLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD 102 Query: 904 PALAPEAGTLDPDG-VWSGSAAYDRDGNPVLFYTAGNDSLSPNQRTGLATPADLSDPNLE 962 AL + + S + L + Sbjct: 103 QALDRKN---IREALHDSLKRLQTDYL------DLY---QVHWPQRPTNCFGKLGYSWTD 150 Query: 963 KWVKYP------------EPVTEQNGNGIHNEFRDPFVWYDKETDKWYQLVTSGLKDFSS 1010 + F + + Sbjct: 151 SAPAVSLLDTLDALAEYQRAGKIRYIG---VSNETAFGVMRYLHLADKHDLPRIVT---- 203 Query: 1011 GTALVYVSDDMYNWEYKGPLYVSDRSLYPELGTVWELPVLLPLGKDSTGKQKYIFMINPH 1070 S ++E + + V L LG + + Sbjct: 204 --IQNPYSLLNRSFEVGL-------AEVSQYEGVELLAYSC-LGFGTLTGKYLNGAKPAG 253 Query: 1071 EKPEQVPPANDVLRDVEVFYWIGTWDRDNFKFIPDQDAPSKMDVG----DGYLTAESGMV 1126 + Y + A ++ + + Sbjct: 254 ARNTLFSRF--------TRYSGEQTQKAVAA--YVDIARRHGLDPAQMALAFVRRQPFVA 303 Query: 1127 TPDGRTVVYSMVQNV----RTPQAEYQAGWAHNLALPVSLSLDEHD---ELRIEPIQELQ 1179 + ++ + Sbjct: 304 STLLGATTMDQLKTNIESLHLELS-- 361 Query: 1180 SLRGAKLVDFADKNLQAANQLIQNVKGDMLEIVMEIDPGQAQKFGLKVRRSDNGQEETLI 1239 Sbjct: 362 --- 418 Query: 1240 YYDKTAGTFNVDRTKSSIDPDVRVDGIQG-GYVDLKGENLKLHIFLDRSVVEAFANDKKK 1298 Sbjct: 419 - 477 Query: 1299 LTTRVYVGRYDSLGLQVWAD------NDITVKSME 1327 Sbjct: 478 512 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.314 0.133 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 147,270,736 Number of extensions: 6523024 Number of successful extensions: 20822 Number of sequences better than 1.0e-05: 12 Number of HSP's gapped: 20790 Number of HSP's successfully gapped: 19 Length of query: 1944 Length of database: 38,378,431 Length adjustment: 121 Effective length of query: 1823 Effective length of database: 24,423,017 Effective search space: 44523159991 Effective search space used: 44523159991 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 123 (52.0 bits)