BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0596.0009.p (317 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH1857 [G] COG0524 Sugar kinases, ribokinase family 191 2e-48 BS_ydjE [G] COG0524 Sugar kinases, ribokinase family 166 4e-41 VCA0656 [G] COG0524 Sugar kinases, ribokinase family 161 1e-39 CAC0424 [G] COG0524 Sugar kinases, ribokinase family 160 3e-39 ECs3242 [G] COG0524 Sugar kinases, ribokinase family 159 4e-39 >BH1857 [G] COG0524 Sugar kinases, ribokinase family Length = 319 Score = 191 bits (484), Expect = 2e-48, Method: Compositional matrix adjust. Identities = 101/232 (43%), Positives = 141/232 (60%), Gaps = 4/232 (1%) Query: 28 ITAIGEVLIDFTPAGLSEKGEQLFECNPGGAPANVVVVLAKLGKSTAFIGKVGEDQFGEY 87 + +GE LIDF P + ++++ +PGGAPANV V LAKLG + F+GKVG+D G + Sbjct: 5 VICLGEALIDFIPM---DSTNEIYQKSPGGAPANVAVGLAKLGAKSTFVGKVGDDLLGRF 61 Query: 88 LTRILLENGVNTKGLVKESRAKTTLAFVHLKEDGDRSFSFYRNPGADMLLGMDDIDLEQI 147 L L + VNT+ ++ +T FV L +G+RSF FY NP AD L +I Sbjct: 62 LKETLTKANVNTEHMLFSKEVRTGATFVTLAANGERSFDFYINPSADRFLEKGEIAPTLF 121 Query: 148 KGTKIFHFGSVSMTHEPSASATLKACIHAKENGALISFDPNLRPALWQDLEQAKIRIKEG 207 + + HFGS+S+ EP+ +AT A AK+NG +IS+DPNLR LW+D EQA++ I Sbjct: 122 EEHGVLHFGSISLISEPARTATRHAVSLAKKNGLMISYDPNLRLNLWEDEEQARLLITSM 181 Query: 208 LALADIVKISEEELEFITGLTDLEEGTRQIQTQFKVKVVLVTKAEKGCFVRF 259 L AD++KISEEEL FITG +E G Q+ + + +V VT EKG V + Sbjct: 182 LGEADVLKISEEELAFITGEQTIEAGLDQL-VAYNIPLVFVTLGEKGSIVSY 232 >BS_ydjE [G] COG0524 Sugar kinases, ribokinase family Length = 452 Score = 166 bits (420), Expect = 4e-41, Method: Compositional matrix adjust. Identities = 12/238 (5%), Positives = 44/238 (18%), Gaps = 9/238 (3%) Query: 28 ITAIGEVLIDFTPAGLS---EKGEQLFECNPGGAPANVVVVLAKLGKSTAFIGKVGEDQF 84 + L + + VV+ + + Sbjct: 6 QIGARLDRLPLARFHYRIFGIISFSLLL-TGFLSYSGNVVLAKLVSNGWSNNFLNAAFTS 64 Query: 85 GEYLTRILLENGVNTKGLVKESRAKTTLAFVHLKEDGDRSFSFYRNPGADMLLGMDDIDL 144 + G R + + + + ++ + Sbjct: 65 ALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAATGAAFVPD--MYWLIFFRFLMGT 122 Query: 145 EQIKGTKIFHFGSVS-MTHEPSASATLKACIHAKENGALISFDPNLRPALWQDLEQAKIR 203 + + + + + + L + + A + + Sbjct: 123 GMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPMLSAAIGVVVIAFFSWRIMFLLG 182 Query: 204 I-KEGLALADIVKISEEELEFITGLTDLEEGTRQIQTQFKVKVVLVTKAEKGCFVRFG 260 LA K E ++ G + + + ++ + Sbjct: 183 GIGILLAWFLSGKYFIESPRWLAGKGQIAGAE-CQLREVEQQIEREKSIRLPPLTSYQ 239 >VCA0656 [G] COG0524 Sugar kinases, ribokinase family Length = 323 Score = 161 bits (407), Expect = 1e-39, Method: Compositional matrix adjust. Identities = 87/242 (35%), Positives = 136/242 (56%), Gaps = 9/242 (3%) Query: 23 NHMVDITAIGEVLIDFTPAGLSEKGEQLFECNPGGAPANVVVVLAKLGKSTAFIGKVGED 82 + M + G+ ++D P G+Q + PGGAPANV V +A+L +AF G+VG D Sbjct: 16 DSMSRVWLTGDAVVDLIP-----DGQQHYLKCPGGAPANVAVAIARLCGRSAFFGRVGND 70 Query: 83 QFGEYLTRILLENGVNTKGLVKESRAKTTLAFVHLKEDGDRSFSFYRNPGADMLLGMDDI 142 FG ++ + L + V+ + L + +T+ V L E G+RSF+F P AD L + DI Sbjct: 71 PFGRFMQQTLTDEQVDCQHLYFDPVHRTSTVVVDLDEHGERSFTFMVKPSADQFLQLSDI 130 Query: 143 DLEQIKGTKIFHFGSVSMTHEPSASATLKACIHAKENGALISFDPNLRPALWQDLEQAKI 202 + + H S+++ ++PS S+T A KE G +SFDPNLR +W + ++ + Sbjct: 131 --PSFQNGEWLHVCSIALANQPSRSSTFAAIAQMKEVGGYVSFDPNLREEVWSEPQELQA 188 Query: 203 RIKEGLALADIVKISEEELEFITGLTDLEEGTRQIQTQFKVKVVLVTKAEKGCFVRFGPG 262 + + LAD+VK SEEEL+F+TG +EEG + I F++ +V+VT KG V P Sbjct: 189 TVMRAVGLADVVKFSEEELQFLTGTQSIEEGLQAI-ADFQIPLVVVTLGAKGALV-VTPN 246 Query: 263 SK 264 S+ Sbjct: 247 SR 248 >CAC0424 [G] COG0524 Sugar kinases, ribokinase family Length = 316 Score = 160 bits (405), Expect = 3e-39, Method: Compositional matrix adjust. Identities = 98/241 (40%), Positives = 146/241 (60%), Gaps = 10/241 (4%) Query: 25 MVDITAIGEVLIDFTPAGLS---EKGEQLFECNPGGAPANVVVVLAKLGKSTAFIGKVGE 81 M ++ IGE+LIDF + + KGE F+ GGAPANV ++KLG S +F+GKVG Sbjct: 1 MNNVLCIGELLIDFICSDIDTTLSKGEN-FKKKAGGAPANVTAAISKLGGSASFLGKVGN 59 Query: 82 DQFGEYLTRILLENGVNTKGLVKESRAKTTLAFVHLKEDGDRSFSFYRNPGADMLLGMDD 141 D FG +L L E V+T L+ ++ + TTLAFV L+ +G+R F F R GAD LL D+ Sbjct: 60 DPFGHFLKETLDEVKVDTSMLIMDNNSSTTLAFVSLQANGERDFVFNR--GADGLLRYDE 117 Query: 142 IDLEQIKGTKIFHFGSVSMTHEPSASAT-LKACIHAKENGALISFDPNLRPALWQDLEQA 200 I+L+++ KI HFGS + + T LK AK+ G +ISFDPN R LW++ + Sbjct: 118 INLDKVYSNKIIHFGSATALLGGEMTDTYLKIMEEAKKRGIIISFDPNYRDNLWENRTEE 177 Query: 201 KIRI-KEGLALADIVKISEEELEFITGLTDLEEGTRQIQTQFKVKVVLVTKAEKGCFVRF 259 I I ++ + LAD VK+S+EEL+ I+G +++ G + + + KV+ VT ++G + Sbjct: 178 FIAISRKCIELADFVKLSDEELKIISGEKNIKNGVKLLASN--NKVIAVTLGKEGTMISN 235 Query: 260 G 260 G Sbjct: 236 G 236 >ECs3242 [G] COG0524 Sugar kinases, ribokinase family Length = 304 Score = 159 bits (403), Expect = 4e-39, Method: Compositional matrix adjust. Identities = 82/232 (35%), Positives = 134/232 (57%), Gaps = 7/232 (3%) Query: 28 ITAIGEVLIDFTPAGLSEKGEQLFECNPGGAPANVVVVLAKLGKSTAFIGKVGEDQFGEY 87 + +G+ ++D P E +L C PGGAPANV V +A+LG ++ FIG+VG+D FG Sbjct: 5 VWVLGDAVVDLLP----ESDGRLLPC-PGGAPANVAVGIARLGGTSGFIGRVGDDPFGAL 59 Query: 88 LTRILLENGVNTKGLVKESRAKTTLAFVHLKEDGDRSFSFYRNPGADMLLGMDDIDLEQI 147 + R LL GV+ L ++ +T+ V L + G+RSF+F P AD+ L + DL Sbjct: 60 MQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVRPSADLFL--ETTDLPCW 117 Query: 148 KGTKIFHFGSVSMTHEPSASATLKACIHAKENGALISFDPNLRPALWQDLEQAKIRIKEG 207 + + H S++++ EPS ++ A + G +SFDPN+R LWQD ++ +++ Sbjct: 118 RHGEWLHLCSIALSAEPSRTSAFTAMTEIRHAGGFVSFDPNIREDLWQDEHLLRLCLRQA 177 Query: 208 LALADIVKISEEELEFITGLTDLEEGTRQIQTQFKVKVVLVTKAEKGCFVRF 259 L LAD+VK+SEEE I+G T + + ++++ ++LVTK +G V + Sbjct: 178 LQLADVVKLSEEEWRLISGKTQNDRDICALAKEYEIAMLLVTKGAEGVVVCY 229 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.138 0.409 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 24,312,203 Number of extensions: 1046096 Number of successful extensions: 1757 Number of sequences better than 1.0e-05: 92 Number of HSP's gapped: 1685 Number of HSP's successfully gapped: 92 Length of query: 317 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 211 Effective length of database: 26,153,027 Effective search space: 5518288697 Effective search space used: 5518288697 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 116 (49.3 bits)