BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0591.0029.p (453 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SMc02513 [F] COG2233 Xanthine/uracil permeases 159 8e-39 CAC0872 [F] COG2233 Xanthine/uracil permeases 157 4e-38 BS_yunK [F] COG2233 Xanthine/uracil permeases 153 5e-37 lin1997 [F] COG2233 Xanthine/uracil permeases 149 8e-36 L160092 [F] COG2233 Xanthine/uracil permeases 142 1e-33 >SMc02513 [F] COG2233 Xanthine/uracil permeases Length = 490 Score = 159 bits (402), Expect = 8e-39, Method: Compositional matrix adjust. Identities = 118/401 (29%), Positives = 207/401 (51%), Gaps = 27/401 (6%) Query: 1 MHSTHSEEQVLTVGIEEKLSIGANVTYGFQHLLALTG-IFLFPVLIGQALGLDASIIGYM 59 M ++ E T +EKL IGAN+ YG QH+L + G I P+++GQA GL + IG + Sbjct: 1 MANSAEETVSPTRPEDEKLGIGANLAYGLQHVLTMYGGIVAVPLILGQAAGLGSGDIGLL 60 Query: 60 IQACFLTTGIVTVLQSGKM----LRLPVVQGPTAAFFVAVLSAGSTVGLGTAFGSMAVAG 115 I A G+ T+LQ+ + +LP+VQG + + +++ GL + G++ A Sbjct: 61 ITASLFAGGLATILQTMGLPFFGSQLPLVQGVSFSGVATMIAISGNGGLQSVLGAVIAAS 120 Query: 116 LIFMLLSIPVRRFGLIGYLNKFISPPIVYGTLLIIIGAQLADIGLKNWFGSADTGINFAA 175 LI +L++ R + +F PP+V G ++ IG L + + G + +F + Sbjct: 121 LIGLLITPIFSR------ITRFF-PPLVTGIVITTIGLTLMPVAARWAMGGNSSAPDFGS 173 Query: 176 SXXXXXXXXXXXXFG-----GNTILRRGALLWGIIAGTIFYSIFGTTNFSSVAAAGWFSF 230 G+ + R ++L +I GT+ G +FS V +F+ Sbjct: 174 PANIQLAAVTLVIVLLLSKLGSAAISRLSILLALIIGTVIAYFAGMADFSQVTEGPFFAL 233 Query: 231 PDVFPFGF-----GVSVPIVILMLLAFLQASAEAVGMYTLLTKWGNQKLDNDRVNRGLFG 285 P VF FG+ + I++++ ++ SA+ + + ++ K+D+ R+ GL Sbjct: 234 PLVFHFGYPTFEVAAIASMFIVIMVTLVETSADILAVGEII----ETKVDSRRLGDGLRA 289 Query: 286 EFLGSTLGAVFGGLGTTSYPENIGIVRVSGIGSRYVTMTAGVLAIVLGLIPKVGMFIASL 345 + L S L +FG +++ +N+G+V V+G+ SRYV T G+ + LGL+P +G +A++ Sbjct: 290 DMLSSLLAPIFGSFTQSAFAQNVGLVAVTGVKSRYVVATGGLFLVTLGLLPVMGRIVAAV 349 Query: 346 PGPVLSAASTILFGIIAISGIQMVSKVVW-DELNLVVAGSS 385 P VL A +LFG +A SGI+ +SKV + + +NLV+ +S Sbjct: 350 PSSVLGGAGIVLFGTVAASGIRTLSKVDYANNMNLVIVATS 390 >CAC0872 [F] COG2233 Xanthine/uracil permeases Length = 435 Score = 157 bits (397), Expect = 4e-38, Method: Compositional matrix adjust. Identities = 115/424 (27%), Positives = 210/424 (49%), Gaps = 29/424 (6%) Query: 2 HSTHSEEQV-LTVGIEEKLSIGANVTYGFQHLLA-LTGIFLFPVLIGQALGLDASIIGYM 59 E Q+ L+ G+E+K + + + FQH+ A GI + P+++ +L LDA + Sbjct: 3 EKIKKETQIELSYGVEDKPELLSRILLAFQHIFAAFGGIIVVPIIVASSLKLDAKTSTAL 62 Query: 60 IQACFLTTGIVTVLQSGKML----RLPVVQGPTAAFFVAVLSAGSTVGLGTAFGSMAVAG 115 + A L G+ T++QS + R+ + G F G GL FG+ + Sbjct: 63 LSAAILMAGVATLIQSKGVYSVGARVACIMGTDITFVAPAAVVGKKFGLAGIFGATILGA 122 Query: 116 LIFMLLSIPVRRFGLIGYLNKFISPPIVYGTLLIIIGAQLADI---------GLKNWFGS 166 +I ++LS I Y+ KF PPIV G ++ +IG L + G KN+ GS Sbjct: 123 VIVVILSF------FIKYIMKFF-PPIVTGIVVCLIGLTLLPVSIDWAAGGSGTKNY-GS 174 Query: 167 ADTGINFAASXXXXXXXXXXXXFGGNTILRRGALLWGIIAGTIFYSIFGTTNFSSVAAAG 226 N + + +G ++ ++L G++ G I G NFS+++ Sbjct: 175 LQ---NISIALIVMVITLLINHYG-RGLVSSASILIGMVVGYIICIPLGMVNFSTISNLK 230 Query: 227 WFSFPDVFPFGFGVSVPIVILMLLAFLQASAEAVGMYTLLTKWGNQKLDNDRVNRGLFGE 286 S P +F +G ++ ++ + A+ + VG + + K D+ ++++G+ + Sbjct: 231 IISIPSIFQYGVKFNISALLPFIPAYFVSVISTVGNLRAINEISGIK-DDSKISKGVLSD 289 Query: 287 FLGSTLGAVFGGLGTTSYPENIGIVRVSGIGSRYVTMTAGVLAIVLGLIPKVGMFIASLP 346 +GS L +FG + TS+ +N+G++ ++ + SRYVT+ AG++ +VLGL+PK I +P Sbjct: 290 GIGSILAGMFGAMPNTSFSQNVGLIPLTKVASRYVTLVAGIILMVLGLVPKFSGIINIMP 349 Query: 347 GPVLSAASTILFGIIAISGIQMVSKVVWDELNLVVAGSSFIISLGTMYLPAELTQNLPLA 406 PVL ++FG +A +GIQ +S V + N+++ +S + LG + P E+ +LP + Sbjct: 350 QPVLGGVGIVMFGTVAAAGIQTLSHVNLNNRNILIIATSIGLGLGVTFRP-EILSSLPES 408 Query: 407 FQSI 410 + I Sbjct: 409 LKMI 412 >BS_yunK [F] COG2233 Xanthine/uracil permeases Length = 211 Score = 153 bits (387), Expect = 5e-37, Method: Compositional matrix adjust. Identities = 0/386 (0%), Positives = 0/386 (0%), Gaps = 28/386 (7%) Query: 28 GFQHLLAL-TGIFLFPVLIGQALGLDASIIGYMIQACFLTTGIVTVLQSGKM----LRLP 82 + F P + A+ L A + V + + + Sbjct: 14 RENNPHPTTELNFSSPFELLIAVLLSAQATDVSVNKATAKLYPVANTPAAMLELGVEGVK 73 Query: 83 VVQGPTAAFFVAVLSAGSTVGLGTAFGSMAVAGLIFMLLSIPVRRFGLIGYLNKFISPPI 142 + + T + + V L + + Sbjct: 74 TYIKTIGLYNSKAENIIKTCRILLEQHNGEVPEDRAALEA------LPGVGRKTAN-VVL 126 Query: 143 VYGTLLIIIGAQLADIGLKNW--------FGSADTGI-NFAASXXXXXXXXXXXXFGGNT 193 I + N + + + Sbjct: 127 NTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVEEKLLKVVPAEFKVDCHHWLILH---- 182 Query: 194 ILRRGALLWGIIAGTIFYSIFGTTNFSSVAAAGWFSFPDVFPFGFGV-SVPIVILMLLAF 252 Sbjct: 183 - 241 Query: 253 LQASAEAVGMYTLLTKWGNQKLDNDRVNRGLFGEFLGSTLGAVFGGLGTTSYPENIGIVR 312 Sbjct: 242 301 Query: 313 VSGIGSRYVTMTAGVLAIVLGLIPKVGMFIASLPGPVLSAASTILFGIIAISGIQMVSKV 372 Sbjct: 302 361 Query: 373 VWD-ELNLVVAGSSFIISLGTMYLPA 397 Sbjct: 362 387 >lin1997 [F] COG2233 Xanthine/uracil permeases Length = 435 Score = 149 bits (377), Expect = 8e-36, Method: Compositional matrix adjust. Identities = 118/369 (31%), Positives = 185/369 (50%), Gaps = 26/369 (7%) Query: 28 GFQHLLAL-TGIFLFPVLIGQALGLDASIIGYMIQACFLTTGIVTVLQSGKM----LRLP 82 GFQH+LA+ G + P+LIG ALG + + Y++ GI T+LQ + LP Sbjct: 11 GFQHVLAMYAGAVIVPLLIGGALGFNGEEMTYLVSIDIFMCGIATLLQLTVNRFFGIGLP 70 Query: 83 VVQGPTAAFFVAVLSAGSTVGLGTAFGSMAVAGLIFMLLSIPVRRFGLIGYLNKFIS--P 140 VV G ++ G +G+G +GS+ V+GL F+LL P + +K + P Sbjct: 71 VVLGCAVQAIAPIILIGQDMGIGAIYGSIIVSGL-FVLLIAP--------FFSKVVRFFP 121 Query: 141 PIVYGTLLIIIGAQLADIGLKNWFGSA---DTGINFAASXXXXXXXXXXXXFG-GNTILR 196 P+V G+++ +IG L + + N G A D G + + G + Sbjct: 122 PVVTGSVVTVIGLTLIPVAINNLAGGAGAKDFGSMYNLGLGFGTLLLIILVYRFGQGFSK 181 Query: 197 RGALLWGIIAGTIFYSIFGTTNFSSVAAAGWFSFPDVFPFGFGVSV---PIVILMLLAFL 253 A+L G++ G++F +++ + V+ A WF P PF FG P +I M+L L Sbjct: 182 AIAVLIGLVGGSLFAALYKGISLGPVSEASWFHMPK--PFYFGTPTFEWPAIITMILIAL 239 Query: 254 QASAEAVGMYTLLTKWGNQKLDNDRVNRGLFGEFLGSTLGAVFGGLGTTSYPENIGIVRV 313 + E+ G+Y L+ +KL + RG E L LG VF T+Y +N+G+V++ Sbjct: 240 VSMVESTGVYFALSDITERKLTQKDLTRGYRAEGLAIMLGGVFNTFPYTAYSQNVGLVQL 299 Query: 314 SGIGSRYVTMTAGVLAIVLGLIPKVGMFIASLPGPVLSAASTILFGIIAISGIQMVSKVV 373 SGI +R V A IVLGLIPK+G +P PVL A +FG++ GI+M+ KV Sbjct: 300 SGIKTRKVIYAAAGFLIVLGLIPKIGAVTTIIPTPVLGGAMVAMFGMVVAQGIKMLGKVN 359 Query: 374 W-DELNLVV 381 + + NL++ Sbjct: 360 FTSQENLLI 368 >L160092 [F] COG2233 Xanthine/uracil permeases Length = 434 Score = 142 bits (358), Expect = 1e-33, Method: Compositional matrix adjust. Identities = 132/416 (31%), Positives = 198/416 (47%), Gaps = 37/416 (8%) Query: 16 EEKLSIGANVTYGFQHLLAL-TGIFLFPVLIGQALGLDASIIGYMIQACFLTTGIVTVLQ 74 ++ S + G QHLLA+ +G L P++I AL A+ + Y+I A G+ T LQ Sbjct: 8 SQEQSNSKSAVLGLQHLLAMYSGSILVPIMIAGALNYSATQLTYLISADIFMCGLATFLQ 67 Query: 75 SGKMLR------LPVVQGPTAAFFVAVLSAGSTVGLGTAFGSMAVAGLIFMLLSIPVRRF 128 LR LPVV G ++ G G G FGS+ VAG+ +L+S Sbjct: 68 --LQLRKHFGIGLPVVLGVAFQSVAPLIIIGQRHGSGAMFGSLMVAGVFVILVS------ 119 Query: 129 GLIGYLNKFISPPIVYGTLLIIIGAQLADIGLKNWFGSADT----GINFAASXXXXXXXX 184 G+ + K PPIV G+++ IG L + + N + D + A S Sbjct: 120 GIFSKIRKLF-PPIVTGSVITTIGLSLIPVAIGNMGNNVDKPTIQSLILAVSTIVIILLI 178 Query: 185 XXXXFGGNTILRRGALLWGIIAGTIFYSIFGTTNFSSVAAAGWFSFPDVFPFGFGVSVPI 244 G +R A+L G+IAGTI + G +FS V+ A W P F F S P Sbjct: 179 NIFTTG---FIRSIAILIGLIAGTILAASMGLVDFSVVSQAPWAHLPQPFYF----SAPK 231 Query: 245 V-----ILMLLAFLQASAEAVGMYTLLTKWGNQKLDNDRVNRGLFGEFLGSTLGAVFGGL 299 ++M++ + + E+ G+Y L + LD R+ G E LG VF Sbjct: 232 FYLADSLMMIIIAIVSLVESTGVYLALADITGENLDEKRLRNGYRAEGFAVFLGGVFNTF 291 Query: 300 GTTSYPENIGIVRVSGIGSRY-VTMTAGVLAIVLGLIPKVGMFIASLPGPVLSAASTILF 358 T + +N+G+V++SGI +R + TAG L IVLGLIPK +P PVL A I+F Sbjct: 292 PYTGFSQNVGLVQLSGIKTRKPIYFTAGFL-IVLGLIPKFAAVAQLIPTPVLGGAMLIMF 350 Query: 359 GIIAISGIQMVSKVVWD-ELNLVVAGSSFIISLGTMYLPAELTQNLPLAFQSIVTQ 413 G++A GI+M++KV ++ NL++A + I++G + L LP Q V+ Sbjct: 351 GMVATQGIRMLAKVEFEGNQNLLIAAVA--IAMGVGFNSTNLFTALPSFIQPFVSN 404 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.325 0.141 0.416 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 30,251,080 Number of extensions: 1272322 Number of successful extensions: 4887 Number of sequences better than 1.0e-05: 69 Number of HSP's gapped: 4735 Number of HSP's successfully gapped: 71 Length of query: 453 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 343 Effective length of database: 25,691,691 Effective search space: 8812250013 Effective search space used: 8812250013 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 117 (49.7 bits)