BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0591.0013.p (612 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH1064 [G] COG1653 ABC-type sugar transport system, periplasmic ... 144 4e-34 BH0482 [G] COG1653 ABC-type sugar transport system, periplasmic ... 65 3e-10 BS_lplA [G] COG1653 ABC-type sugar transport system, periplasmic... 62 4e-09 SP1796 [G] COG1653 ABC-type sugar transport system, periplasmic ... 58 5e-08 YPO3472 [G] COG1653 ABC-type sugar transport system, periplasmic... 56 2e-07 >BH1064 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 550 Score = 144 bits (363), Expect = 4e-34, Method: Compositional matrix adjust. Identities = 155/587 (26%), Positives = 256/587 (43%), Gaps = 76/587 (12%) Query: 55 MKRRL---GMIAVLLISLTTNACSNSKNSNSDASQHAKLLPGSSGKYEEPVTIHIGTTVE 111 MK+R+ ++ +L + + T ACS ++ D + + + VTI T++ Sbjct: 1 MKKRIVHSFLVGLLAVGILT-ACSQEGSTTEDETNTNE---------DGTVTITTARTLD 50 Query: 112 STKSLLQGETLVDNQYTRNIKNNLNI-IVDYSFKASPANYNQKVSLAIASGD-LPDAMVV 169 GE + DN TR + L I V + + +N ++ L ++SG+ LPD V Sbjct: 51 DGTVFKDGEDVHDNVVTRWAEEELGIKFVTEWTQPNDEQFNTQLRLMMSSGEKLPDVFQV 110 Query: 170 GAVELK-EMVEADQLADLTEVYNTYASPVIKRIVDSTGGEALGSVTFNGKILALPNVQLQ 228 +K +++ + + + E TYASP +K++ + +A T +G +P Sbjct: 111 NDTAIKADLLSSGLVMPVDEAIETYASPRLKQLFEQFP-QAFYPSTKDGVRYGIPRFSGG 169 Query: 229 ADGIHXXXXXXXXXXXXXXXPPQTMEELEAVAHAFVEQDPDGNGQRDTIGLAGPSATGRM 288 PP T+EELE + FV +DP+ G+ DTIG+ + Sbjct: 170 NGSDTLLWIRQDWLDKFDLEPPTTLEELEHIMDVFVNEDPNETGKNDTIGITFAAGDYGF 229 Query: 289 YANFLQSTNNLYGFDGIFAAYNAYPGYWLE-NNGKVVYGSIQPETKEALGILRKMYLSGA 347 + ++ ++G G F PG W + ++G +VYGSIQP K+ L + + Y G Sbjct: 230 RGGNIADSSWVFGAFGDFV-----PGAWSKGDDGSLVYGSIQPSMKDGLAKIHEWYEKGY 284 Query: 348 IDPEMGVRE--DPGESVASGKTGMFFAPWWAPYGPIDDAVRNNPEANWQAYALPLDKSGS 405 + + + D ES SG++G+ AP WA PI + + P+A + + Sbjct: 285 LATDFAILSAGDAQESFISGRSGIMAAPPWAHGYPIGEMAQVVPDAIVKPF--------- 335 Query: 406 YRPHMSNTTSQ-------FVV----VRKGYEHPEAAMLIVNNLVLGESRGTFTTERGPTE 454 PH+S Q +VV K +EH + + + G + G E E Sbjct: 336 --PHVSGPNGQAGRRGEGYVVGSFLFNKDFEHMD-KFFEYWDAIYGYTFG----ESDYFE 388 Query: 455 YPL--RLTYAAAD-ETEYEVKALRQVFAGTKTAEDFLNKPEYKMLQSDIRNIGPVKLGPY 511 Y L Y D E Y+ ++ T E+ ++ Y L +++ I P+ Sbjct: 389 YGLFEGYDYVMEDGEPVYDHAQFEEI-----TGEEVVDPGRY-FLPTNVPTI------PF 436 Query: 512 DKYDIQYWEPDKNRAMWVRAYSLMVGGSP-------LVD--NEIKGIFSATYTQTPTMES 562 + Y + + R + + G P +V+ N+++ I TPTM S Sbjct: 437 EMYSLLEEFHETGREPETGYEAGLAGRDPDYIEAAAIVNRQNDLRIINEFNGPPTPTMVS 496 Query: 563 RWLKLQRLEDETFLKIILGAAPLEAFDHFAEEWKRQGGEEITREVNE 609 R L+R+EDE F+ II G PL+AFD F EEW++ GGE IT EVNE Sbjct: 497 RSEHLERMEDELFVDIIYGNKPLDAFDQFVEEWRQSGGEAITEEVNE 543 >BH0482 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 512 Score = 65.5 bits (158), Expect = 3e-10, Method: Compositional matrix adjust. Identities = 110/492 (22%), Positives = 183/492 (37%), Gaps = 115/492 (23%) Query: 153 KVSLAIASGDLPDAMVVGAVELKEMVEADQLADLTEVYNTYASPVIKRI--VDSTGGEAL 210 +V+ A+ASG+LP + + ++ A + E+ P + + L Sbjct: 95 RVNAALASGNLPKVITLQDFRTSSVLNAFRSGQFWEI-----GPYLDEFPNLSEMNETIL 149 Query: 211 GSVTFNGKI--------LALPNVQLQADGIHXXXXXXXXXXXXXXXPPQTMEELEAVAHA 262 +V+ +GKI L+ V ++ D + P +EEL VA A Sbjct: 150 ENVSVDGKIYGIYRERPLSRQGVVIRQDWLENLGLEM----------PTNLEELYEVARA 199 Query: 263 FVEQDPDGNGQRDTIGLAGPSATGRMYANFLQSTNNLYGFDGIFAAYNAYPGYW-LENNG 321 F DP+G+G DT+G F + ++G A+Y+ P W ++ G Sbjct: 200 FTYDDPNGSGADDTVG-------------FTDRNDLIFGAFKTLASYHGTPNEWGFDDEG 246 Query: 322 KVVYGSIQPETKEALGILRKMYLSGAIDPEMGV--REDPGESVASGKTGMFFAPWWAPYG 379 K++ + E E + ++ +Y G I+ E V +E E +G+ G++ G Sbjct: 247 KLMPEFMTDEYVETMNYMKSLYDEGLINTEFPVTSKEQQQELFMTGRAGIYV-------G 299 Query: 380 PIDDAVRNNPEANWQAYALPLDKSGSYRPHMSNTTSQFVVVRKGYEHPEAAMLIVNNLVL 439 + DA RN DK+ P M + + G + A Sbjct: 300 NMVDA-RN-----------LRDKAKDINPEMEVAITNRIAGPDGEDRVWAQ--------- 338 Query: 440 GESRGTFTTERGPTEYPLRLTYAAADETEYEVKALRQVFAGTKTAEDFLNKPEYKMLQSD 499 G G F + ETE E+K + F + AE+ + Y ++ Sbjct: 339 GGHNGIFAIPKSSV------------ETEAELKRILAFF--DRIAEEDV----YNLMAYG 380 Query: 500 IRNIGPVKLGPYDKYDIQYWEP-DKNRAMWVRAYSLMVG------------GSPLVD--- 543 I ++ Y+ D +Y+E ++N W +V G PL + Sbjct: 381 IEDVH------YETIDGEYFERIEENLDDWAAEVQPLVSLVAREMNYLKAYGDPLYEEYE 434 Query: 544 ------NEIKGIFSATYTQTPTMESRWLKLQRLEDETFLKIILGAAPLEAFDHFAEEWKR 597 NEI A +PT R +LQ++ D+ K ILG + F+ E W Sbjct: 435 RLTEDNNEIIVTNPAEPLFSPTYTERGTELQKIIDDATYKYILGELDEDGFEAEVERWLT 494 Query: 598 QGGEEITREVNE 609 GGE I E+NE Sbjct: 495 NGGESIIEELNE 506 >BS_lplA [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 502 Score = 61.6 bits (148), Expect = 4e-09, Method: Compositional matrix adjust. Identities = 122/575 (21%), Positives = 215/575 (37%), Gaps = 97/575 (16%) Query: 54 KMKRRLGMIAVLLISLTTNACSNSKNSNSDASQHAKLLPGSSGKYEEPVTIHIGTTVEST 113 K++ R +A+ L ++ CS+S+ SNS +SG + T++ Sbjct: 2 KIRMRKKWMALPLAAMMIAGCSHSETSNS-----------ASGSKD---------TIKIM 41 Query: 114 KSLLQGETLVDNQYTRNIKNNLNIIVDYSFK------ASPANYNQKVSLAIASGDLPDAM 167 LL E+ D + L + Y+ K ++YN K ++ +ASG++P A+ Sbjct: 42 APLLSPESPSDKSPS------LKALEKYTGKEIKVTWVPDSSYNDKFNIVMASGEMPHAI 95 Query: 168 VVG--AVELKEMVEADQLADLTEVYNTYA--SPVIKRIV--DSTGGEALGSVTFNGKILA 221 V+ + + V+A +L+ Y S ++I+ S GE G + + L Sbjct: 96 VIKDKSAGFIKSVKAGAFWELSPYLKDYKNLSQADEKILKNSSVNGEVYG--IYRTRDLI 153 Query: 222 LPNVQLQADGIHXXXXXXXXXXXXXXXPPQTMEELEAVAHAFVEQDPDGNGQRDTIGLAG 281 + ++ D + P+T+++ V AF E+DPDGNG+ DT G+ Sbjct: 154 RACMIIRTDWLKNVGLDM----------PETLDDFYEVLKAFKEKDPDGNGKDDTYGMVV 203 Query: 282 PSATGRMYANFLQSTNNLYGFDGIFAAYNAYPGYWLENNGKVVYGSIQPETKEALGILRK 341 P G N +D + + A Y +E NGK++ E +AL +K Sbjct: 204 PKWMG---------LGNGSPWDVLQIWFGAPNRYGVE-NGKLIPDFTTKEYMDALTFFKK 253 Query: 342 MYLSGAIDPEMGVREDP--GESVASGKTGMFFAPWWAPYGPIDDAVRNNPEANWQAYAL- 398 +Y G I+ + V + + V GK G+ + I A+ E+N + Sbjct: 254 LYDEGLINKDFAVMDSAKWNDPVVKGKAGVIVDT-GSRASQIQSAMEEADESNKDIIDIV 312 Query: 399 -PLDKSGSYRPHMSNTTSQFVVVRKGYEHPEAAMLIVNNLV--LGESRGTFTTERGPTEY 455 L+ R ++ S + + K E + V + + + + T G Sbjct: 313 GSLEGPNGKRTFPTSGYSGMITIPKSSVKTEKELKEVLSFLDKMNDKEAQILTNNGVKGR 372 Query: 456 PLRLTYAAADETEYEVKALRQVFAGTKTAEDFLNKPEYKMLQSDIRNIGPVKLGPYDKYD 515 L E K+L G + K EY Sbjct: 373 NYELKDGVFTSLEKNNKSLLYEHEGLAQFSMSIPKSEY---------------------- 410 Query: 516 IQYWEPDKNRAMWVRAYSLMVGGSPLVDNEIKGIFSATYTQTPTMESRWLKLQRLEDETF 575 Y E K + R + G V N + + S YTQ + +L + + Sbjct: 411 --YIEDQKTKLFQHRKDIITEGEKIAVFNPAESLVSDVYTQ------KGAQLDNIILDAR 462 Query: 576 LKIILGAAPLEAFDHFAEEWKRQGGEEITREVNEL 610 + I+G + FD E WK+ GG E+ +++N+L Sbjct: 463 TQFIIGEIDEKGFDDAVELWKKSGGNELMKDLNKL 497 >SP1796 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 538 Score = 57.8 bits (138), Expect = 5e-08, Method: Compositional matrix adjust. Identities = 80/323 (24%), Positives = 131/323 (40%), Gaps = 34/323 (10%) Query: 63 AVLLI-SLTTNACSNSKNSNSDASQHAKLLPGSSGKYEEPVTIHIGTTVESTKSLLQGET 121 AVLL SL A SKN+ AS L G + +E T+ T E Sbjct: 10 AVLLTASLAVLAACGSKNT---ASSPDYKLEGVTFPLQEKKTLKFMTASSPLSPKDPNEK 66 Query: 122 LVDNQYTRNIKNNLNIIVDYSFKASPANYNQKVSLAIASGDLPDAMV---VGAVELKEMV 178 L+ + ++ + +D++ S ++ +K +L I+SGDLPDA+ V+L Sbjct: 67 LI----LQRLEKETGVHIDWTNYQS--DFAEKRNLDISSGDLPDAIHNDGASDVDLMNWA 120 Query: 179 EADQLADLTEVYNTYASPVIKRIVDSTGGEALGSVTFNGKILALPNVQLQADG---IHXX 235 + + + ++ + Y P +K+I+D +G I + P ++ DG IH Sbjct: 121 KKGVIIPVEDLIDKYM-PNLKKILDEKPEYKALMTAPDGHIYSFPWIEELGDGKESIHSV 179 Query: 236 XXXXXXXXXXXXX----PPQTMEELEAVAHAFVEQDPDGNGQRDTIGLAGPSATGRMYAN 291 P+T ++L V AF DP+GNG+ D I + S G Sbjct: 180 NDMAWINKDWLKKLGLEMPKTTDDLIKVLEAFKNGDPNGNGEADEIPFSFISGNGNEDFK 239 Query: 292 FLQSTNNLYGFDGIFAAYNAYPGYWLENNGKVVYGSIQPETKEALGILRKMYLSGAIDPE 351 FL + + D + N+GKV + + KE + +R++ G ID E Sbjct: 240 FLFAAFGIGDNDDHLV---------VGNDGKVDFTADNDNYKEGVKFIRQLQEKGLIDKE 290 Query: 352 MGVREDPGESVASG---KTGMFF 371 D +A G K G++F Sbjct: 291 -AFEHDWNSYIAKGHDQKFGVYF 312 >YPO3472 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 437 Score = 55.8 bits (133), Expect = 2e-07, Method: Compositional matrix adjust. Identities = 42/132 (31%), Positives = 58/132 (43%), Gaps = 16/132 (12%) Query: 250 PQTMEELEAVAHAFVEQDPDGNGQRDTIGLAGPSATGRMYAN-FLQSTNNLYGFDGIFAA 308 P+ +++ +A AF DPDGNG+ DT G P +T R YA+ F+ S G D I A Sbjct: 161 PKNWQDISTLAKAFTFNDPDGNGKNDTYGFILPGSTTRGYASWFISSYLWQAGGDFIRPA 220 Query: 309 YNAYPGYWLENNGKVVYGSIQPETKEALGILRKMYLSGAIDPEM--GVREDPGESVASGK 366 GK + +P E L +R M + P D S SG+ Sbjct: 221 ----------GEGKFIASLEEPAATETLSFMRGMVCDKTVQPGAINATTADAIPSFRSGQ 270 Query: 367 TGMFFAPWWAPY 378 +GMFF+ PY Sbjct: 271 SGMFFS---GPY 279 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.315 0.133 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 44,729,094 Number of extensions: 1841864 Number of successful extensions: 2173 Number of sequences better than 1.0e-05: 6 Number of HSP's gapped: 2170 Number of HSP's successfully gapped: 7 Length of query: 612 Length of database: 38,378,431 Length adjustment: 112 Effective length of query: 500 Effective length of database: 25,461,023 Effective search space: 12730511500 Effective search space used: 12730511500 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 119 (50.4 bits)