BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0591.0001.p (325 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value YPO2336 [I] COG0657 Esterase/lipase 62 2e-09 mll0618 [I] COG0657 Esterase/lipase 55 1e-07 Rv0220 [I] COG0657 Esterase/lipase 53 5e-07 MT0230 [I] COG0657 Esterase/lipase 53 5e-07 SA0610 [I] COG0657 Esterase/lipase 52 1e-06 >YPO2336 [I] COG0657 Esterase/lipase Length = 334 Score = 61.6 bits (148), Expect = 2e-09, Method: Compositional matrix adjust. Identities = 40/126 (31%), Positives = 67/126 (53%), Gaps = 7/126 (5%) Query: 51 EMGTSYTDIAYGDGE-ANKFDLYVPADHTKEAYGLVVYLHAGGFTTGDKSDDA-SMLKWL 108 + Y D++Y + + K D+++P + K Y +++ +H G F DK D+ + + Sbjct: 54 RIKNKYLDVSYANASPSEKMDIFLP-EQWKLRYPVIINVHGGAFFGCDKMDNQLTPALYG 112 Query: 109 ASKGYVAAGINYTLRDEVHPEASVYSQSMEIKSSIPAVVEEAKKLGYNLDSMAISGGSAG 168 SKGY INY L PEA+ +Q ++K++I V A K ++ D++ + GGSAG Sbjct: 113 LSKGYAVVNINYRLS----PEAAWPAQINDLKAAIKFVRANAIKYNFDPDNIILMGGSAG 168 Query: 169 GTLAML 174 G L L Sbjct: 169 GNLVAL 174 >mll0618 [I] COG0657 Esterase/lipase Length = 301 Score = 55.5 bits (132), Expect = 1e-07, Method: Compositional matrix adjust. Identities = 8/121 (6%), Positives = 16/121 (13%), Gaps = 15/121 (12%) Query: 53 GTSYTDIAYGDGEANKFDLYVPADHTKEAYGLVVYLHAGGFTTGDKSDDASMLKWLASKG 112 Y D + G + + Sbjct: 52 TEGAYQAIYVDTPGLHMEEKRA---INRLMNKAASSSIGDVELVIFVVEGTRWTPDDEMV 108 Query: 113 YVAAGINYTLRDEVHPEASVYSQSMEIKSSIPAVVEEAKKLGYNLDS-MAISGGSAGGTL 171 A +++ K+ + L S M Sbjct: 109 LNKLREGKAP----VILAVNKVDNVQEKADL-------LPHLQFLASQMNFLDIVPISAE 157 Query: 172 A 172 Sbjct: 158 T 158 >Rv0220 [I] COG0657 Esterase/lipase Length = 403 Score = 53.1 bits (126), Expect = 5e-07, Method: Compositional matrix adjust. Identities = 54/248 (21%), Positives = 103/248 (41%), Gaps = 21/248 (8%) Query: 61 YGDGEANKFDLYVPADHTKEAYGLVVYLHAGGFTTGDKSDDA-SMLKWLASKGYVAAGIN 119 YGD A D++ D + +++++ G + G ++ ++L LA++G+V I+ Sbjct: 135 YGDDPAQLLDVWRRKDMPTKPAPVLIFVPGGAWVHGSRAIQGYAVLSRLAAQGWVCLSID 194 Query: 120 YTLRDEVHPEASVYSQSMEIKSSIPAVVEEAKKLGYNLDSMAISGGSAGGTLAMLYAYRD 179 Y V P +++K++I K G + + +A++G SAGG L+ L Sbjct: 195 Y----RVAPHHRWPRHILDVKTAIAWARANVDKFGGDRNFIAVAGCSAGGHLSALAGLTA 250 Query: 180 AD-----ESPIPVKMLFEA-VGPSSFYPEDWSVYGLDQNPEAAANLFSIMAGTDIKADLL 233 D E P +A VG Y DW D++ A + ++ + Sbjct: 251 NDPQYQAELPEGSDTSVDAVVGIYGRY--DWE----DRSTPERARFVDFLERVVVQRTI- 303 Query: 234 GTEAYEEAIKPISAYMWVNEHSVPTVAAYGVYDRVCPFDTVKHLINALEKNNVTH-EYIE 292 + + E + S V ++ P + +G D V P + + + L + + Y+E Sbjct: 304 --DRHPEVFRDASPIQRVTRNAPPFLVIHGSRDCVIPVEQARSFVERLRAVSRSQVGYLE 361 Query: 293 FPHSGHAL 300 P +GH Sbjct: 362 LPGAGHGF 369 >MT0230 [I] COG0657 Esterase/lipase Length = 403 Score = 53.1 bits (126), Expect = 5e-07, Method: Compositional matrix adjust. Identities = 54/248 (21%), Positives = 103/248 (41%), Gaps = 21/248 (8%) Query: 61 YGDGEANKFDLYVPADHTKEAYGLVVYLHAGGFTTGDKSDDA-SMLKWLASKGYVAAGIN 119 YGD A D++ D + +++++ G + G ++ ++L LA++G+V I+ Sbjct: 135 YGDDPAQLLDVWRRKDMPTKPAPVLIFVPGGAWVHGSRAIQGYAVLSRLAAQGWVCLSID 194 Query: 120 YTLRDEVHPEASVYSQSMEIKSSIPAVVEEAKKLGYNLDSMAISGGSAGGTLAMLYAYRD 179 Y V P +++K++I K G + + +A++G SAGG L+ L Sbjct: 195 Y----RVAPHHRWPRHILDVKTAIAWARANVDKFGGDRNFIAVAGCSAGGHLSALAGLTA 250 Query: 180 AD-----ESPIPVKMLFEA-VGPSSFYPEDWSVYGLDQNPEAAANLFSIMAGTDIKADLL 233 D E P +A VG Y DW D++ A + ++ + Sbjct: 251 NDPQYQAELPEGSDTSVDAVVGIYGRY--DWE----DRSTPERARFVDFLERVVVQRTI- 303 Query: 234 GTEAYEEAIKPISAYMWVNEHSVPTVAAYGVYDRVCPFDTVKHLINALEKNNVTH-EYIE 292 + + E + S V ++ P + +G D V P + + + L + + Y+E Sbjct: 304 --DRHPEVFRDASPIQRVTRNAPPFLVIHGSRDCVIPVEQARSFVERLRAVSRSQVGYLE 361 Query: 293 FPHSGHAL 300 P +GH Sbjct: 362 LPGAGHGF 369 >SA0610 [I] COG0657 Esterase/lipase Length = 347 Score = 52.4 bits (124), Expect = 1e-06, Method: Compositional matrix adjust. Identities = 32/119 (26%), Positives = 55/119 (46%), Gaps = 6/119 (5%) Query: 56 YTDIAYGDGEAN-KFDLYVPADHTKEAYGLVVY-LHAGGFTTGDKSDDASMLKWLASKGY 113 Y ++ Y N K D+ P D + A V++ +H GG+ GDK +L +A +GY Sbjct: 54 YQNLTYNRVFPNSKLDIITPVDMSSNAKLPVIFWMHGGGYIAGDKQYKNPLLAKIAEQGY 113 Query: 114 VAAGINYTLRDEVHPEASVYSQSMEIKSSIPAVVEEAKKLGYNLDSMAISGGSAGGTLA 172 + +NY L P+ + +++ + + E L + + + I G SAG LA Sbjct: 114 IVVNVNYALA----PQYKYPTPLIQMNQATQFIKENKMNLPIDFNQVIIGGDSAGAQLA 168 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.315 0.132 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 25,776,804 Number of extensions: 1124790 Number of successful extensions: 1687 Number of sequences better than 1.0e-05: 11 Number of HSP's gapped: 1701 Number of HSP's successfully gapped: 11 Length of query: 325 Length of database: 38,378,431 Length adjustment: 107 Effective length of query: 218 Effective length of database: 26,037,693 Effective search space: 5676217074 Effective search space used: 5676217074 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 116 (49.3 bits)