BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0589.0037.p (57 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value L1009 [R] COG0561 Predicted hydrolases of the HAD superfamily 50 9e-07 MA0318 [R] COG0561 Predicted hydrolases of the HAD superfamily 49 3e-06 L106425 [R] COG0561 Predicted hydrolases of the HAD superfamily 47 6e-06 >L1009 [R] COG0561 Predicted hydrolases of the HAD superfamily Length = 270 Score = 49.7 bits (117), Expect = 9e-07, Method: Compositional matrix adjust. Identities = 26/52 (50%), Positives = 36/52 (69%) Query: 3 FGDGYNDLELLVRAAYSFAVRNAVPELKEAANYIIGSNDDNAVMKTIIHMLS 54 FGD NDLE+ A SFAV NA PE+ E A+ +I SND++AV+ I ++L+ Sbjct: 217 FGDQMNDLEMFEYAGSSFAVSNAAPEILELADKVILSNDEDAVLVEIENILN 268 >MA0318 [R] COG0561 Predicted hydrolases of the HAD superfamily Length = 134 Score = 48.5 bits (114), Expect = 3e-06, Method: Compositional matrix adjust. Identities = 22/48 (45%), Positives = 34/48 (70%) Query: 3 FGDGYNDLELLVRAAYSFAVRNAVPELKEAANYIIGSNDDNAVMKTII 50 FGD ND+++ A + AV NA PELK+ A IIG+N++++V+K I+ Sbjct: 68 FGDNVNDIKMFRLARHKIAVSNAKPELKKYATLIIGTNEEDSVVKYIM 115 >L106425 [R] COG0561 Predicted hydrolases of the HAD superfamily Length = 243 Score = 47.4 bits (111), Expect = 6e-06, Method: Compositional matrix adjust. Identities = 23/51 (45%), Positives = 32/51 (62%) Query: 1 MAFGDGYNDLELLVRAAYSFAVRNAVPELKEAANYIIGSNDDNAVMKTIIH 51 AFGDG NDL+L A + A+ NAVPELKE A++I N ++ ++ H Sbjct: 189 FAFGDGRNDLDLFKAADHRTAMGNAVPELKELADFISTENTNHGIINAFNH 239 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.135 0.371 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 4,047,223 Number of extensions: 118469 Number of successful extensions: 2117 Number of sequences better than 1.0e-05: 4 Number of HSP's gapped: 2124 Number of HSP's successfully gapped: 4 Length of query: 57 Length of database: 38,378,431 Length adjustment: 30 Effective length of query: 27 Effective length of database: 34,918,411 Effective search space: 942797097 Effective search space used: 942797097 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 109 (46.6 bits)