BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0589.0036.p (421 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CAC0847 [V] COG0534 Na+-driven multidrug efflux pump 107 3e-23 VC1631 [V] COG0534 Na+-driven multidrug efflux pump 69 1e-11 BB0583 [V] COG0534 Na+-driven multidrug efflux pump 52 2e-06 >CAC0847 [V] COG0534 Na+-driven multidrug efflux pump Length = 459 Score = 107 bits (268), Expect = 3e-23, Method: Compositional matrix adjust. Identities = 90/365 (24%), Positives = 151/365 (41%), Gaps = 11/365 (3%) Query: 22 NRTFNRELVHLVIPIALQNLIAATAVSADVVMLGAVGXXXXQCRRYHWQDKSLLFLPCFI 81 ++ F + L IPI +QN I ++ D +++G +G Q LL L F Sbjct: 9 DKKFFKAAFGLAIPIIIQNFILSSLNLVDNIIIGGLGETAIAGVGLANQYFFLLDLALF- 67 Query: 82 WGCXXXXXGILAAQYWGKKDVHTIQRVLNIACXXXXXXXXXXXXXXXXXPNELMYFFTQD 141 I AQYWGK+D I+RVL I PN ++ FF+QD Sbjct: 68 --GTASGASIFTAQYWGKRDTKNIKRVLGICLITTIILSGIFTIGALAFPNIIIGFFSQD 125 Query: 142 EELIRYGAKFLHTISFSYLAMGLTQMFLGVIXXXXXEQAQLSALISSSGLFLNILLNALC 201 + +I GA +L I FSY+ G++ F I Q ++ ++S + L +N LN Sbjct: 126 KNVISLGANYLRIICFSYIVTGIS--FAYSIILRTVGQVKIPMIVSGTALAINTFLNFSL 183 Query: 202 VFVLFPNMPEKAITSVALATVVARFFE--LGWCYIYSITRGTVRFQLPERDDAQRKLLKD 259 V+ + + + A+AT++ARF E L IY + + + +L E D +K Sbjct: 184 VYGI-GSFHGIGVYGSAIATLIARFIEFFLMLLAIYKL-KLPIAAKLHELLDLSTTFVKR 241 Query: 260 YLRYTTPVQGNYIVWGGAXXXXPPAAIIGHINADMXXXXXXXXXXXXXXXXXCGGIATGG 319 + + PV N +WG AA+ H+ + G Sbjct: 242 FFKVAVPVMLNESMWG--LGVTAYAAVYAHMGTAVIAATNITSTIDRLSMVVFFGFGNAA 299 Query: 320 SVLVGKYLGNNEIEMAKKAGARMIWYALIFGVLSGGLILLMKPLVFYAVDLNQNAQEYLH 379 ++++G +G + + A R I + G+ G ++ P + YA ++N Y Sbjct: 300 AIMIGNRIGQKDEKTAFLYAKRFIILSPFIGIFMGIILYTSSPAILYAYNINSQVHMYSK 359 Query: 380 GMLYI 384 +L+I Sbjct: 360 EILHI 364 >VC1631 [V] COG0534 Na+-driven multidrug efflux pump Length = 458 Score = 69.3 bits (168), Expect = 1e-11, Method: Compositional matrix adjust. Identities = 82/369 (22%), Positives = 147/369 (39%), Gaps = 18/369 (4%) Query: 25 FNRELVHLVIPIALQNLIAATAVSADVVMLGAVGXXXXQCRRYHWQDKSLLFLPCFIWGC 84 F R L+ + +PI LQN++ ++ DV+MLG +G + F+ + Sbjct: 15 FFRRLIAIALPITLQNIMFSSRGLVDVLMLGQLGEAEIAAVGVAARAT---FVTTIMLVG 71 Query: 85 XXXXXGILAAQYWGKKDVHTIQRVLNIACXXXXXXXXXXXXXXXXXPNELMYFFTQDEEL 144 +L AQYWG D ++ + P ++M T E++ Sbjct: 72 VTTGGALLTAQYWGAGDRKGVRESTALTWLVSNFFAFFTVLLFLFFPQQIMSLTTDSEQV 131 Query: 145 IRYGAKFLHTISFSYLAMG-LTQMFLGVIXXXXXEQAQLSALISSSGLFLNILLNALCVF 203 G+++L SF+ A+ ++ M +G+ + +S S G+ N++LN + +F Sbjct: 132 NALGSQYLIITSFTMFAVACVSSMAVGL---RAMHKPGVSTFFSGIGIVSNVVLNWILIF 188 Query: 204 VLFPNMPEKAITSVALATVVARFFELGWCYIYSITRGTVRFQLPERDDAQRKL----LKD 259 F P I A+ATV++ E+ C +YS G D + + + Sbjct: 189 GKF-GFPAMGIQGAAIATVLSGAIEIA-C-LYSYLYGKKHLMAFGWQDIKAVIDWPKISR 245 Query: 260 YLRYTTPVQGNYIVWGGAXXXXPPAAIIGHINADMXXXXXXXXXXXXXXXXXCGGIATGG 319 +LR + P N+++W A AI+G G++T Sbjct: 246 FLRLSLPTTFNFLIW--AAGLFTYHAIMGQTGVQGLAALSVMTPVESISLSLLIGMSTAA 303 Query: 320 SVLVGKYLGNNEIEMAKKAGARMIWYALIFG-VLSGGLILLMKPLVFYAVDLNQNAQEYL 378 +VLVG LG E + +++ G V++ GL L+ P++ L +E Sbjct: 304 AVLVGNQLGAKNYEAVYYQAIGVTACSVLVGIVVAIGLYLVQIPVLGAFTALTPETRELS 363 Query: 379 HG-MLYICI 386 ML +C+ Sbjct: 364 EKFMLILCL 372 >BB0583 [V] COG0534 Na+-driven multidrug efflux pump Length = 449 Score = 52.0 bits (123), Expect = 2e-06, Method: Compositional matrix adjust. Identities = 52/257 (20%), Positives = 105/257 (40%), Gaps = 12/257 (4%) Query: 19 KNFNRTFNRELVHLVIPIALQNLIAATAVSADVVMLGAVGXXXXQCRRYHWQDKSLLFLP 78 KN+++ +EL + IP A ++L+ D M+ +G + L F+ Sbjct: 6 KNYDQII-KELFVIAIPTAFESLLFQMVTFFDNFMISYLGSFHVTGVSLANRVTFLFFII 64 Query: 79 CFIWGCXXXXXGILAAQYWGKKDVHTIQRVLNIACXXXXXXXXXXXXXXXXXPNELMYFF 138 F G +Q KK +++ P ++ F Sbjct: 65 VFGLGTALSAY---VSQAIAKKKFLQVRQSFAYILLIGTTIGIIFFIFSFLFPRNIIKLF 121 Query: 139 TQDEELIRYGAKFLHTISFSYLAMGLTQMFLGVIXXXXXEQAQLSALISSSGLFLNILLN 198 T +++ + +G+++L IS SY+ M + FL + ++ ++ ++S + +NI+ N Sbjct: 122 TANQDSLNFGSEYLKIISLSYVLMAYS--FLSAMGFKSAKEVKIPLYVTSIVVLINIVFN 179 Query: 199 ALCVFVLFPNMPEKAITSVALATVVARFFELGWCYIYSITRGTVRFQLPERD-DAQRKLL 257 + +F I A ATV+AR E + ++YS+ +++ D A + + Sbjct: 180 YILIFGF-----SMGIKGAAYATVLARIVEFVFYFLYSLISSNSYYRIKFGDFFAPKVVT 234 Query: 258 KDYLRYTTPVQGNYIVW 274 + L+ PV + I W Sbjct: 235 RANLKLIIPVLSHEIFW 251 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.328 0.142 0.441 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 24,075,619 Number of extensions: 799645 Number of successful extensions: 1692 Number of sequences better than 1.0e-05: 4 Number of HSP's gapped: 1687 Number of HSP's successfully gapped: 4 Length of query: 421 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 312 Effective length of database: 25,807,025 Effective search space: 8051791800 Effective search space used: 8051791800 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 117 (49.7 bits)