BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0589.0028.p (286 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH1970 [S] COG2323 Predicted membrane protein 175 7e-44 BH1572 [S] COG2323 Predicted membrane protein 152 6e-37 BS_ydfS [S] COG2323 Predicted membrane protein 145 5e-35 CAP0075 [S] COG2323 Predicted membrane protein 143 3e-34 CAC1521 [S] COG2323 Predicted membrane protein 128 1e-29 >BH1970 [S] COG2323 Predicted membrane protein Length = 292 Score = 175 bits (443), Expect = 7e-44, Method: Compositional matrix adjust. Identities = 92/257 (35%), Positives = 156/257 (60%), Gaps = 2/257 (0%) Query: 26 GKRQVSQLSFFEYITGITVGSLAAYISLDTDKTWHLGVIALIVWVAFSLGIEFLQMKSKK 85 GK+ ++ ++++EYI GI +GS+AA ++ + +I+W F + + L++KS + Sbjct: 26 GKKHITDMTYYEYIVGIAIGSIAAELTFSPHVRMSNFLFGMIIWAIFPMVLSKLELKSFR 85 Query: 86 ARDFIDFKSTVLIKDGKILEDNMKKERLTTDELLGELRKKDVFNVADVEFAIMETDGAIN 145 R + + TVLI++G ILE+N+KKE LT DEL+ LR+KD F + DVE A+ME G I+ Sbjct: 86 FRSLSEGEPTVLIENGNILEENLKKENLTVDELMIHLRQKDAFKLDDVESAVMEKSGLIS 145 Query: 146 VLLTRENQPLTPKHLGVKVAPEQETQAVIMDGEIMDEPLDALGLTRGWLMGELEKLNLSP 205 V+ QPLTPK G+ + + Q V++DG ++++ L G T+ WL+ E+ K Sbjct: 146 VMKKSNVQPLTPKDAGLVIELDHSPQIVMIDGNVLEKSLQDYGYTKEWLLAEVTKQGAKR 205 Query: 206 -ENVYLAQVDSYGELTVDLYTD-SVEVPQTQEKPQLYALLKKCEADLELFSLSTNNEQAK 263 E+V+LAQ+DS G + VDLY D +P+ ++K + A +K+ +ADL S+ + + K Sbjct: 206 FEDVFLAQIDSKGNVYVDLYNDQQSNMPEVKQKLLMAANIKQLQADLIQISMQVTDAKTK 265 Query: 264 KMYGQCSEQLQGLLKQL 280 + + ++Q+ L +L Sbjct: 266 EQFKAHAKQMDQLFHEL 282 >BH1572 [S] COG2323 Predicted membrane protein Length = 283 Score = 152 bits (384), Expect = 6e-37, Method: Compositional matrix adjust. Identities = 93/285 (32%), Positives = 148/285 (51%), Gaps = 2/285 (0%) Query: 1 MPEWLEVVWRTXXXXXXXXXXXXXXGKRQVSQLSFFEYITGITVGSLAAYISLDTDKTWH 60 M WLE R+ G+R +S F+ I + +A+ S + Sbjct: 1 MAGWLEAFIRSVCSIIVLFLVFKAYGRRSFQDVSLFQGAMIIALAGIASIGSFLLTVPFM 60 Query: 61 LGVIALIVWVAFSLGIEFLQMKSKKARDFIDFKSTVLIKDGKILEDNMKKERLTTDELLG 120 +AL++W A LG LQ K A F + +++ GKI ED + K++LT ++ + Sbjct: 61 PFFVALLLWSAVFLGAHRLQGKHGGALKFFAGQPETVVEKGKIKEDVLHKQKLTAEDFMR 120 Query: 121 ELRKKDVFNVADVEFAIMETDGAINVLLTRENQPLTPKHLGVKVAPEQETQAVIMDGEIM 180 +LR+K++F ++DVE+A +ET+GA+ V L +E G K Q VI +G+I+ Sbjct: 121 KLREKNIFQLSDVEWAQLETNGALTVFL-KEGSYSEAASTG-KQGRRQPPMTVIQNGQIV 178 Query: 181 DEPLDALGLTRGWLMGELEKLNLSPENVYLAQVDSYGELTVDLYTDSVEVPQTQEKPQLY 240 D L G+TR WL ELEK + NV+LA++D G+L VDLY D + E P L Sbjct: 179 DSALQKRGVTREWLKAELEKQEVLLGNVFLAEMDQNGQLYVDLYNDDITPKPVNELPLLR 238 Query: 241 ALLKKCEADLELFSLSTNNEQAKKMYGQCSEQLQGLLKQLKPFVQ 285 +KK +ADL +L++ N K+Y +C+E +Q + + P+V+ Sbjct: 239 ESIKKAQADLAYAALTSTNRTNAKVYSRCAEAMQQVENVVSPYVR 283 >BS_ydfS [S] COG2323 Predicted membrane protein Length = 235 Score = 145 bits (367), Expect = 5e-35, Method: Compositional matrix adjust. Identities = 86/232 (37%), Positives = 133/232 (57%), Gaps = 1/232 (0%) Query: 5 LEVVWRTXXXXXXXXXXXXXXGKRQVSQLSFFEYITGITVGSLAAYISLDTDKTWHLGVI 64 LEVV RT GK+ +SQ++ F++I IT+G++AA ++ +T H I Sbjct: 4 LEVVIRTVASFGLLLIAERILGKQTISQMTIFDFIAAITLGAIAAGLAYNTSIKPHNMAI 63 Query: 65 ALIVWVAFSLGIEFLQMKSKKARDFIDFKSTVLIKDGKILEDNMKKERLTTDELLGELRK 124 + ++V I FL +K++K R F TVLI++GKILE NM+K R T D L +LR+ Sbjct: 64 SFSIFVLTIFLISFLSIKNRKLRKFFAGDPTVLIQNGKILESNMRKMRYTLDYLNQQLRE 123 Query: 125 KDVFNVADVEFAIMETDGAINVLLTRENQPLTPKHLGVKVAPEQETQA-VIMDGEIMDEP 183 K++FN+ +V FAI+ET+G + VL + + +T + L + V EQ +IMDGEI++ Sbjct: 124 KEIFNIEEVLFAILETNGQLTVLRKPQFRHVTKQDLMIAVNQEQRLPIELIMDGEIIENN 183 Query: 184 LDALGLTRGWLMGELEKLNLSPENVYLAQVDSYGELTVDLYTDSVEVPQTQE 235 L LT WL+ EL K ++ + A + G++ VD Y D + VP +E Sbjct: 184 LKQNRLTESWLLEELRKRDIKVKETVYAVLLGNGDIYVDQYKDHISVPMDKE 235 >CAP0075 [S] COG2323 Predicted membrane protein Length = 233 Score = 143 bits (360), Expect = 3e-34, Method: Compositional matrix adjust. Identities = 76/218 (34%), Positives = 129/218 (59%), Gaps = 1/218 (0%) Query: 5 LEVVWRTXXXXXXXXXXXXXXGKRQVSQLSFFEYITGITVGSLAAYISLDTDKTWHLGVI 64 + V+R+ G++ +SQ++FF+++ G+++GS+ + T V Sbjct: 6 INTVFRSVIVYLIAMFLTRLMGRKLISQMTFFDFVMGVSMGSIITDSIVGQKFTSISSVT 65 Query: 65 ALIVWVAFSLGIEFLQMKSKKARDFIDFKSTVLIKDGKILEDNMKKERLTTDELLGELRK 124 AL V + ++ +KS K R+ I+ K +L+ +G I+E+NMK+ R+T +ELL +LR+ Sbjct: 66 ALTVLSILVIITGYIHIKSLKLREIINSKPIILVDNGNIVEENMKESRITINELLMKLRE 125 Query: 125 KDVFNVADVEFAIMETDGAINVLLTRENQPLTPKHLGVKVAPEQETQAVIMDGEIMDEPL 184 K++FN+ADVEFAI+E DG ++VL +PLTP ++ + V TQ +I+DG I+DE L Sbjct: 126 KNIFNMADVEFAIIEIDGQLSVLPKASQKPLTPSNMNINVTSTGLTQDIIIDGNILDENL 185 Query: 185 DALGLTRGWLMGELEKLNLSPEN-VYLAQVDSYGELTV 221 GL WL+ +L N+ E+ V+ A +D+ +L V Sbjct: 186 YKAGLDIDWLLSQLNSQNIKNESEVFYAGIDNTKKLYV 223 >CAC1521 [S] COG2323 Predicted membrane protein Length = 242 Score = 128 bits (322), Expect = 1e-29, Method: Compositional matrix adjust. Identities = 78/231 (33%), Positives = 130/231 (56%), Gaps = 3/231 (1%) Query: 1 MPEWLEVVWRTXXXXXXXXXXXXXXGKRQVSQLSFFEYITGITVGSLAAYISLD-TDKTW 59 M E L V+ R+ GK+Q+SQL+FF+YI GIT+GS+AA ++ D + + W Sbjct: 1 MNEGLVVIVRSIIGFFTMLIFAKILGKQQISQLTFFDYILGITIGSIAATLTTDLSSRAW 60 Query: 60 HLGVIALIVWVAFSLGIEFLQMKSKKARDFIDFKSTVLIKDGKILEDNMKKERLTTDELL 119 V L+ W +E++ K + A +++ + T++I GKI+ED MKK +++ Sbjct: 61 PHWV-GLLTWALLGYLMEYISTKWRYAGKYLEGEPTIVIMKGKIMEDAMKKMNFRATDIM 119 Query: 120 GELRKKDVFNVADVEFAIMETDGAINVLLTRENQPLTPKHLGVKVAPEQETQAVIMDGEI 179 LR KDVF++ +V+FAI+E +G ++VL E QP+T + + + +I DG + Sbjct: 120 ELLRDKDVFDLNEVDFAIIEPNGKLSVLKKPELQPVTCNDMNITKNEMGISTELIYDGIL 179 Query: 180 MDEPLDALGLTRGWLMGELEKLNL-SPENVYLAQVDSYGELTVDLYTDSVE 229 ++E L L T+ WL LE + + V+LA ++ G L +DLY D ++ Sbjct: 180 IEENLRQLNKTKDWLFKVLESRGIKNVSEVFLATLNPGGSLYIDLYKDHIK 230 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.135 0.385 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 19,346,994 Number of extensions: 755879 Number of successful extensions: 1560 Number of sequences better than 1.0e-05: 16 Number of HSP's gapped: 1541 Number of HSP's successfully gapped: 16 Length of query: 286 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 181 Effective length of database: 26,268,361 Effective search space: 4754573341 Effective search space used: 4754573341 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 115 (48.9 bits)