BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0589.0019.p (308 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value mll1869 [I] COG0657 Esterase/lipase 126 4e-29 RSc0439 [I] COG0657 Esterase/lipase 112 8e-25 mlr0240 [I] COG0657 Esterase/lipase 110 3e-24 PA2098 [I] COG0657 Esterase/lipase 109 5e-24 ML0314 [I] COG0657 Esterase/lipase 102 8e-22 >mll1869 [I] COG0657 Esterase/lipase Length = 301 Score = 126 bits (317), Expect = 4e-29, Method: Compositional matrix adjust. Identities = 21/300 (7%), Positives = 48/300 (16%), Gaps = 9/300 (3%) Query: 6 SSIEMLTAIKNHLKQNNQTAGKTAMQIRLELAESA-KRLQQLPF-ISAEKADMNGLQGEW 63 S + + T + L S R Q Q + Sbjct: 2 SIDKS-YCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTRHRIVGIHTEGAYQAIY 60 Query: 64 VNACKGHNPEDRRKVILYFHGGGFISGSCEVYRDLAARISAASGVPVLTVEYRLAPEHVY 123 V G + + S +L + + Sbjct: 61 V-DTPG---LHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWTPDDEMVLNKLREGK 116 Query: 124 PAANDDCYSAYCRLLETGLQPEDIILGGDSVGATLALMTMITLRESGGRLPAAAFLLSPH 183 + L P L + ++ T L Sbjct: 117 APVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGLNVDTIAAIVRKHLPEA 176 Query: 184 GDLVHLDGESYDSNAELDPTGSREGNRRILRDYLGGYAGEPPALLSXXXXXXXXXXXXXI 243 + D + + Y+ + Sbjct: 177 THHF-PEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI-ERFVSNERGGYDINGL 234 Query: 244 QAGDQEVLLSDAEKLAERIRAERGEVTLEIWENMWSVFQMMSAMLPEAQEAIGNIGSFVR 303 ++E G + + M+ + + + + Sbjct: 235 ILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLELWVKVKSGWADDERALRS 294 >RSc0439 [I] COG0657 Esterase/lipase Length = 317 Score = 112 bits (280), Expect = 8e-25, Method: Compositional matrix adjust. Identities = 82/254 (32%), Positives = 117/254 (46%), Gaps = 5/254 (1%) Query: 53 KADMNGLQGEWVNACKGHNPEDRRKVILYFHGGGFISGSCEVYRDLAARISAASGVPVLT 112 +AD L GEW+ + D V+ Y HGGG+ S +R + ++ + V Sbjct: 53 RAD-GALPGEWIEPADSDSLGDAPPVVFYLHGGGYFFCSPRTHRSITIGLAVHARARVFA 111 Query: 113 VEYRLAPEHVYPAANDDCYSAYCRLLETGLQPEDIILGGDSVGATLALMTMITLRESGGR 172 ++YRLAPEH +PAA D + Y LL + P I++ GDS G L+L ++ LR++G Sbjct: 112 LDYRLAPEHPFPAAVLDALAGYRGLLAQRVAPSRIVVAGDSAGGGLSLALLVALRDAGDP 171 Query: 173 LPAAAFLLSPHGDLVHLDGESYDSNAELDPTGSREGNRRILRDYLG--GYAGEPPALLSX 230 LPA A L SP DL G S N D R YLG G + P L S Sbjct: 172 LPAGAILYSPWTDLAA-TGASLVENDASDVMFRGAWMARGATLYLGDSGVPADHP-LASP 229 Query: 231 XXXXXXXXXXXXIQAGDQEVLLSDAEKLAERIRAERGEVTLEIWENMWSVFQMMSAMLPE 290 EVL D ++AER RA V++E+ + V+ + LPE Sbjct: 230 LYADFTGLPPLHCYVSTSEVLRDDTLRMAERARAAGVSVSVELGRRLPHVWPIFYPFLPE 289 Query: 291 AQEAIGNIGSFVRK 304 A++A+ G VR+ Sbjct: 290 ARKALRQSGDAVRR 303 >mlr0240 [I] COG0657 Esterase/lipase Length = 301 Score = 110 bits (275), Expect = 3e-24, Method: Compositional matrix adjust. Identities = 16/259 (6%), Positives = 50/259 (19%), Gaps = 11/259 (4%) Query: 49 ISAEKADMNGLQGEWVNACKGHNPEDRRKVILYFHGGGFISGSCEVYRDLAARISAASGV 108 + + ++ + ++ I V + Sbjct: 51 HTEGAYQAIYVDTPGLHMEEKRAINR----LMNKAASSSIGDVELVIFVVEGTRWTPDDE 106 Query: 109 PVLTVEYRLAPEHVYPAANDDCYSAYCRLLETGLQPEDIILGGDSVGATLALMTMITL-- 166 VL + D LL ++ T + + Sbjct: 107 MVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQ--FLASQMNFLDIVPISAETGLNVDT 164 Query: 167 -RESGGRLPAAAFLLSPHGDLVHLDGESYDSNAELDPTGSREGNRRILRDYLGGYAGEPP 225 + A P + + + ++ + R + Sbjct: 165 IAAIVRKHLPEATHHFPEDYIT-DRSQRFMASEIIREKLMRFLGAELPYSVTVEIERFVS 223 Query: 226 ALLSXXXXXXXXXXXXXIQAGDQEVLLSDAEKLAERIRAERGEVTLEIWENMWSVFQMMS 285 + A+ I A + + ++ + Sbjct: 224 N-ERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLELWVKVK 282 Query: 286 AMLPEAQEAIGNIGSFVRK 304 + + + A+ ++G Sbjct: 283 SGWADDERALRSLGYVDDL 301 >PA2098 [I] COG0657 Esterase/lipase Length = 309 Score = 109 bits (273), Expect = 5e-24, Method: Compositional matrix adjust. Identities = 83/251 (33%), Positives = 118/251 (47%), Gaps = 7/251 (2%) Query: 52 EKADMNGLQGEWVNACKGHNPEDRRKVILYFHGGGFISGSCEVYRDLAARISAASGVPVL 111 E+ + G+ EW+ + E RR+V LY HGG ++ GS +R + ++ V Sbjct: 53 ERRSLAGVPCEWL---RPRGVEARRRV-LYLHGGAYLIGSPATHRAITTHLARRCAAEVC 108 Query: 112 TVEYRLAPEHVYPAANDDCYSAYCRLLETGLQPEDIILGGDSVGATLALMTMITLRESGG 171 V+YR APEH +PAA DD + Y LLE G P ++L GDS G LAL + L+ G Sbjct: 109 AVDYRRAPEHPFPAARDDALAVYLALLEAGHSPRRLLLAGDSAGGHLALSLALELKARGL 168 Query: 172 RLPAAAFLLSPHGDLVHLDGESYDSNAELDPTGSREGNRRILRDYLGGYAGEPPALLSXX 231 LPA L SP D L + + A DP SR +R L A A LS Sbjct: 169 PLPAGLLLFSPWTD---LGCQRLHTPAAGDPLLSRAWLESAVRLLLPADAEPGSAALSPL 225 Query: 232 XXXXXXXXXXXIQAGDQEVLLSDAEKLAERIRAERGEVTLEIWENMWSVFQMMSAMLPEA 291 +Q G+ E+L D+ +LA+R + V L+ + W VFQ + +L A Sbjct: 226 HADLAGLPPLLVQVGEDELLRDDSLRLAQRAGEQGVAVRLQRYAGCWHVFQAHAGVLASA 285 Query: 292 QEAIGNIGSFV 302 A+ +FV Sbjct: 286 DRALDEAAAFV 296 >ML0314 [I] COG0657 Esterase/lipase Length = 357 Score = 102 bits (254), Expect = 8e-22, Method: Compositional matrix adjust. Identities = 17/266 (6%), Positives = 44/266 (16%), Gaps = 9/266 (3%) Query: 46 LPFISAEKADMN--GLQGEWVNACKGHNPEDRRKVILYFHGGGFISGSCEVYRDLAARIS 103 ++ + E + + + + ++ Sbjct: 59 NFLSLSKLVEKEFTLKMKEGLASGQALSELLESLSFSKNVVTQLALVEVHGNLSGTMQLV 118 Query: 104 AASGVPVLTVEYRLAPEHVYPAANDDCYSAYCRLLETGLQPEDIILGGDSVGATLALMTM 163 L V+ +L YP L+ L P+ + L + Sbjct: 119 ELHLKKQLKVKNKLVEVATYPILLLIFLVGIMWGLKNYLLPQLNSGSNFATLLINHLPLV 178 Query: 164 ITLRESGGRLPAAAFLLSPHGDLVHLDGESYDSNAELD---PTGSREGNRRILRDYLG-- 218 + A ++ Sbjct: 179 FFSFGAFIF-LLTALSVTLFKRKSA-IMNFTFLVKLPLVHSFIRLYLTAYFAREWGNLIA 236 Query: 219 GYAGEPPALLSXXXXXXXXXXXXXIQAGDQEVLLSDAEKLAERIRAERGEVTLEIWENMW 278 + + E+ ++ E++L I Sbjct: 237 QGVELRQIINLMKKQKSRIFSEVGKNLDLELNAGRSFEQAVSKLALFLPELSLMIEYGAI 296 Query: 279 SVFQMMSAMLPEAQEAIGNIGSFVRK 304 + L + R Sbjct: 297 KDKLGLELSLYADECWEQFFTKIDRL 322 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.134 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 21,895,383 Number of extensions: 866807 Number of successful extensions: 1713 Number of sequences better than 1.0e-05: 40 Number of HSP's gapped: 1695 Number of HSP's successfully gapped: 40 Length of query: 308 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 202 Effective length of database: 26,153,027 Effective search space: 5282911454 Effective search space used: 5282911454 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 116 (49.3 bits)