BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0589.0012.p (255 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH3701 [G] COG1874 Beta-galactosidase 112 8e-25 TM0310 [G] COG1874 Beta-galactosidase 89 9e-18 STM0310 [G] COG0279 Phosphoheptose isomerase 89 9e-18 BH2022 [G] COG1874 Beta-galactosidase 87 3e-17 BS_lacA [G] COG1874 Beta-galactosidase 85 1e-16 >BH3701 [G] COG1874 Beta-galactosidase Length = 672 Score = 112 bits (279), Expect = 8e-25, Method: Compositional matrix adjust. Identities = 77/250 (30%), Positives = 125/250 (50%), Gaps = 12/250 (4%) Query: 8 WFKALQKRHIPNDVLYLRSKTTLEDLIPYQVLIYPHPAIMSDETAGLLDQYVQQGGTLIF 67 ++ AL +IP D++ + EDL Y+++I P ++ A + ++VQ GGT + Sbjct: 430 YYAALFDENIPVDMIGVD-----EDLSKYEIVIAPVLYMVKSGYADRVKEFVQNGGTFVT 484 Query: 68 GCRTGYKDTRGQCYMRPFPGAARDLCGITVEEFTMVQGTRLPTSIRWGNDPEALTG---A 124 +G + + +PG RDL GI VEE + I ND +LTG Sbjct: 485 TFFSGIVNEHDLVTLGGYPGELRDLLGIWVEEIDALPPEE-KNQIVITNDTGSLTGTYEC 543 Query: 125 DAFNDILHVEEDTVEVMGTYASDYYAGKPAVTRNRRGKGEVWYYGAVFNAEAAHKILDSL 184 DI+H E +V+ Y SD+YAG P +TR+ GKG+ +Y G + + ++ Sbjct: 544 RLLFDIIHSE--GADVLAEYGSDFYAGTPVITRHAYGKGKAYYVGTCPDQAFLTDFMKTV 601 Query: 185 NLSSPAAEWLELPPEVELQIRSNDKSGYAFLLNYSEEAVTITLKEPKTDLLTGEILSGSW 244 A L +P VE+ R D + Y F++N++ V + + E TDLLTG+ L+G+ Sbjct: 602 CAEKEIAPLLNVPKGVEVTERRKDGASYFFIMNHNASTVELEIGE-GTDLLTGKELTGAT 660 Query: 245 KLEGFGVRVL 254 LE +GV ++ Sbjct: 661 SLEAYGVMIV 670 >TM0310 [G] COG1874 Beta-galactosidase Length = 672 Score = 88.6 bits (218), Expect = 9e-18, Method: Compositional matrix adjust. Identities = 70/250 (28%), Positives = 112/250 (44%), Gaps = 12/250 (4%) Query: 6 KEWFKALQKRHIPNDVLYLRSKTTLEDLIPYQVLIYPHPAIMSDETAGLLDQYVQQGGTL 65 +++FKAL K + DV+ ++ Y+VL+ P ++ E A L YV GG L Sbjct: 431 QKYFKALVKTGLGVDVV-----GKDQNFERYRVLVAPVLYMVGKELAQKLKDYVSNGGIL 485 Query: 66 IFGCRTGYKDTRGQCYMRPFPGAARDLCGITVEEFTMVQGTRLPTSIRWGNDPEALTGAD 125 I +G D Q + +PG RDL G VEE + + +G E D Sbjct: 486 ILTTMSGIVDEDDQVVLGGYPGYLRDLMGGYVEEIDALPPEEKNEILMFGKRYECSLVFD 545 Query: 126 AFNDILHVEEDTVEVMGTYASDYYAGKPAVTRNRRGKGEVWYYGAVFNAEAAHKILDSLN 185 +++ T EV+G + DYY +PAV RN GKG V+Y G+ + + ++ + Sbjct: 546 ------YIKTTTAEVLGRFGRDYYMNEPAVLRNNYGKGWVYYVGSSTSFDLVWDLVKFME 599 Query: 186 LSSPAAEWLELPPEVELQIRSNDKSGYAFLLNYSEEAVTITLKEPK-TDLLTGEILSGSW 244 + P +VE+ + + + FL N+S E + L E DL+ G + Sbjct: 600 REHNLRAEIAPPDDVEVIKKIKGEKIFYFLFNHSHELQIVDLPEGMFRDLIKGNLYEKKV 659 Query: 245 KLEGFGVRVL 254 KL+ V +L Sbjct: 660 KLQPLDVLIL 669 >STM0310 [G] COG0279 Phosphoheptose isomerase Length = 192 Score = 88.6 bits (218), Expect = 9e-18, Method: Compositional matrix adjust. Identities = 0/250 (0%), Positives = 0/250 (0%), Gaps = 12/250 (4%) Query: 6 KEWFKALQKRHIPNDVLYLRSKTTLEDLIPYQVLIYPHPAIMSDETAGLLDQYVQQGGTL 65 Sbjct: 431 ----- 485 Query: 66 IFGCRTGYKDTRGQCYMRPFPGAARDLCGITVEEFTMVQGTRLPTSIRWGNDPEALTGAD 125 Sbjct: 486 545 Query: 126 AFNDILHVEEDTVEVMGTYASDYYAGKPAVTRNRRGKGEVWYYGAVFNAEAAHKILDSLN 185 Sbjct: 546 ------ 599 Query: 186 LSSPAAEWLELPPEVELQIRSNDKSGYAFLLNYSEEAVTITLKEPK-TDLLTGEILSGSW 244 Sbjct: 600 659 Query: 245 KLEGFGVRVL 254 Sbjct: 660 669 >BH2022 [G] COG1874 Beta-galactosidase Length = 689 Score = 86.7 bits (213), Expect = 3e-17, Method: Compositional matrix adjust. Identities = 66/254 (25%), Positives = 118/254 (46%), Gaps = 13/254 (5%) Query: 3 QSNKEWFKALQKRHIPNDVLYLRSKTTLEDLIPYQVLIYPHPAIMSDETAGLLDQYVQQG 62 Q+ ++ ++ +R IP DV+ T ++ Y++LI P + ++ET L YV G Sbjct: 440 QTLQQHYRTFWERDIPVDVI-----TKEQNFSAYRLLIVPMLYLANEETVARLKSYVANG 494 Query: 63 GTLIFGCRTGYKDTRGQCYMRPFPGAARDLCGITVEEFTMVQGTRLPTSIRWGNDPEALT 122 GTL+ +G + Y +P +D+ G+ E T P+ + + Sbjct: 495 GTLVMTYISGIVNEYDLTYTGGWPKDFQDIFGMRPLE----TDTLYPSDRNYVTYQDKTY 550 Query: 123 GADAFNDILHVEEDTVEVMGTYASDYYAGKPAVTRNRRGKGEVWYYGAVFNAEAAHKILD 182 + I+ +E E G Y D+YA PAVT + G+ +Y GA + E Sbjct: 551 PIKDYATIIDLESAQAE--GLYKEDFYAKLPAVTSHSYKGGKAYYIGARLDDEFHRDFYS 608 Query: 183 SL--NLSSPAAEWLELPPEVELQIRSNDKSGYAFLLNYSEEAVTITLKEPKTDLLTGEIL 240 L +LS + + P V +Q R + + Y F++N++EE T+T++ DL+T E + Sbjct: 609 RLMTDLSLTSVLLVNHGPGVSVQARRDTVTDYVFVMNFTEEKQTVTIQSEVQDLVTKENV 668 Query: 241 SGSWKLEGFGVRVL 254 + L+ + V++L Sbjct: 669 PAEFTLKPYEVKIL 682 >BS_lacA [G] COG1874 Beta-galactosidase Length = 687 Score = 85.1 bits (209), Expect = 1e-16, Method: Compositional matrix adjust. Identities = 63/255 (24%), Positives = 120/255 (47%), Gaps = 15/255 (5%) Query: 3 QSNKEWFKALQKRHIPNDVLYLRSKTTLEDLIPYQVLIYPHPAIMSDETAGLLDQYVQQG 62 Q+ ++ ++ + IP DV+ T +D PY++LI P ++S++T L + G Sbjct: 441 QTLQQHYRTFWEHDIPVDVI-----TKEQDFSPYKLLIVPMLYLISEDTVSRLKAFTADG 495 Query: 63 GTLIFGCRTGYKDTRGQCYMRPFPGAARDLCGIT-VEEFTMVQGTRLPTSIRWGNDPEAL 121 GTL+ +G + Y + + + G+ +E T+ R S R + Sbjct: 496 GTLVMTYISGVVNEHDLTYTGGWHPDLQAIFGVEPLETDTLYPKDRNAVSYR-----SQI 550 Query: 122 TGADAFNDILHVEEDTVEVMGTYASDYYAGKPAVTRNRRGKGEVWYYGAVFNAEAAHKIL 181 + ++ V+ +VE + Y D+YA PAVT + +G+ ++ GA + Sbjct: 551 YEMKDYATVIDVKTASVEAV--YQEDFYARTPAVTSHEYQQGKAYFIGARLEDQFQRDFY 608 Query: 182 DSL--NLSSPAAEWLELPPEVELQIRSNDKSGYAFLLNYSEEAVTITLKEPKTDLLTGEI 239 + L +LS + V +Q R + + Y F++N++EE +T + D++TG+I Sbjct: 609 EGLITDLSLSPVFPVRHGKGVSVQARQDQDNDYIFVMNFTEEKQLVTFDQSVKDIMTGDI 668 Query: 240 LSGSWKLEGFGVRVL 254 LSG +E + VR++ Sbjct: 669 LSGDLTMEKYEVRIV 683 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.136 0.417 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 20,604,803 Number of extensions: 887095 Number of successful extensions: 1234 Number of sequences better than 1.0e-05: 5 Number of HSP's gapped: 1224 Number of HSP's successfully gapped: 5 Length of query: 255 Length of database: 38,378,431 Length adjustment: 104 Effective length of query: 151 Effective length of database: 26,383,695 Effective search space: 3983937945 Effective search space used: 3983937945 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 114 (48.5 bits)