BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0589.0005.p (462 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_ytrF [V] COG0577 ABC-type antimicrobial peptide transport sys... 91 5e-18 MJ1507 [V] COG0577 ABC-type antimicrobial peptide transport syst... 62 2e-09 MA1744 [V] COG0577 ABC-type antimicrobial peptide transport syst... 54 4e-07 SSO1810 [V] COG0577 ABC-type antimicrobial peptide transport sys... 52 2e-06 >BS_ytrF [V] COG0577 ABC-type antimicrobial peptide transport system, permease component Length = 289 Score = 90.5 bits (223), Expect = 5e-18, Method: Compositional matrix adjust. Identities = 0/474 (0%), Positives = 0/474 (0%), Gaps = 51/474 (10%) Query: 1 VRILDVTRLAWGQIKRRKVVTGLCMLGLSIGCSAIVVALSLGDSVQSYVSREVTAQFKMD 60 + + R ++ + + + V + ++ Sbjct: 1 MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKDVTGHAIGAYIRARRLS 60 Query: 61 EITVMPNEGVSQGGGPTATKTGANSNVGKLTEQKLDIIRGLKHVTAAAPFQDAGYFELLT 120 + V + + Q + F Sbjct: 61 KSAVALRLT--------------ARPILDIALQYRFDSQQTFTRAFKKQFAQT------P 100 Query: 121 TDNKIS-SFNLVVTDLSLLTKYDFSFQQGVPSKQIGSVVLNYGATIGLMDVETQKKLMES 179 + S ++ L + + + ++ + ++ + + Sbjct: 101 ALYRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR 160 Query: 180 LSSNSYDPEVMRQYDELSRMPSNLYRQQVQFRHSDYDNSTGGVKSYLSSQLQVAGILKKS 239 + L + D Sbjct: 161 YQFWHDFLGNAPTIPPVLY---GLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPV 217 Query: 240 DGMSADQAMYDKKIYVSQETYDRLKDEFNIKPDKPETGQTYNQALIRVDNQDNLPQVETL 299 Q + + + Sbjct: 218 ----MLQGGEYVMFTYEGLGTGVQEFILTVYGTCMPM----LNLTR----RKGQDIERYY 265 Query: 300 IKKLTLN---TSNNLYQLEQLKSQFAMFKMVALGVGGFXXXXXXXXXXVAMTMSTHQRRR 356 + Sbjct: 266 PAEDAKAGDR 325 Query: 357 QIGIMKVLGSNMRQIRTMFIMEAAXXXXXXXXXXXXXSYLIVEGINKLVSSSGQMTMGT- 415 Sbjct: 326 ---- 381 Query: 416 --GESLVVSIPLNSIPVGIVF--AVLTG---VLSGIYPAISAARTNALTAIKRD 462 Sbjct: 382 435 >MJ1507 [V] COG0577 ABC-type antimicrobial peptide transport system, permease component Length = 289 Score = 61.6 bits (148), Expect = 2e-09, Method: Compositional matrix adjust. Identities = 0/480 (0%), Positives = 0/480 (0%), Gaps = 107/480 (22%) Query: 1 VRILDVTRLAWGQIKRRKVVTGLCMLGLSIGCSAIVVALSLGDSVQSYVSREVTAQFKMD 60 I D+ G + + + + + + R K Sbjct: 5 GIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKDVTGHAIGAYIRARR-LSKSA 63 Query: 61 EITVMPNEGVSQGGGPTATKTGANSNVGKLTEQKLDIIRGLKHVTAAAPFQDAGYFELLT 120 + + + R K A + Sbjct: 64 VALRLT---ARPILDIALQY---------RFDSQQTFTRAFKKQFAQT-PALYRRSPEWS 110 Query: 121 TDNKISSFNLVVTDLSLLTKYDFSFQQGVPSKQIGSVVLNYGATIGLMDVETQKKLMESL 180 + + + Sbjct: 111 AFGI---------------RP-PLRLGEFTMP-----------------------EHKFV 131 Query: 181 SSNSYDPEVMRQYDELSRMPS--------NLYRQQVQFRHSDYDNSTGGVKSYLSSQLQV 232 + + Q S + Sbjct: 132 TLEDTPLIGVTQSYSCSLEQISDFRHEMRYQFWHDFL---------GNAPTIPPVLYGLN 182 Query: 233 AGILKKSDGMSADQAMYDKKIYVSQETYDRLKDEFNIKPDKPETGQ--TYNQALIRVDNQ 290 + + + D+ + + L + Sbjct: 183 ETRPSQDKDDEQEVFYT-----------------TALAQDQADGYVLTGHPVMLQGGEYV 225 Query: 291 DNLPQVETLIKKLTLNTSNN----LYQLEQL----KSQFAMFKMVALGVGGFXXXXXXXX 342 + + + T + L + ++ + G Sbjct: 226 MFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRPINLRCELLI 285 Query: 343 XXVAMTMSTHQRRRQIGIMKVLGSNMRQIRTMFIMEAAXXXXXXXXXXXXXSYLIVEGIN 402 Sbjct: 286 345 Query: 403 KLVSSSGQMTMGTGES--LVVSIPLNSIPVGIVFAVLTGVLSGIYPAISAARTNALTAIK 460 Sbjct: 346 -------- 397 >MA1744 [V] COG0577 ABC-type antimicrobial peptide transport system, permease component Length = 289 Score = 54.3 bits (129), Expect = 4e-07, Method: Compositional matrix adjust. Identities = 0/195 (0%), Positives = 0/195 (0%), Gaps = 21/195 (10%) Query: 278 QTYNQALIRVDNQDNLPQVETLIKKLTLNTSNNLYQLEQLKS-------QFAMFKMVALG 330 Q V LT+ + + A Sbjct: 216 PVMLQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDR 275 Query: 331 VGGFXXXXXXXXXXVAMTMSTHQRRRQIGIMKVLGSNMRQIRTMFIMEAAXXXXXXXXXX 390 Sbjct: 276 PINLRCEL------- 328 Query: 391 XXXSYLIVEGINK----LVSSSGQMTMGTGESLVVSIPLNSIPVGIVFAVLTGVLSGIYP 446 Sbjct: 329 --- 385 Query: 447 AISAARTNALTAIKR 461 Sbjct: 386 400 >SSO1810 [V] COG0577 ABC-type antimicrobial peptide transport system, permease component Length = 289 Score = 52.0 bits (123), Expect = 2e-06, Method: Compositional matrix adjust. Identities = 0/117 (0%), Positives = 0/117 (0%), Gaps = 9/117 (7%) Query: 352 HQRRRQIGIMKVLGSNMRQIRTMFIMEAAXXXXXXXXXXXXXSYL--IVEGINKLVSSSG 409 Sbjct: 282 341 Query: 410 QMTMGTGESLVVSI----PLNSIPVGIVFAVLTGVLSGIYPAISAARTNALTAIKRD 462 Sbjct: 342 --- 395 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.132 0.359 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 28,702,376 Number of extensions: 1132306 Number of successful extensions: 3087 Number of sequences better than 1.0e-05: 14 Number of HSP's gapped: 3108 Number of HSP's successfully gapped: 14 Length of query: 462 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 352 Effective length of database: 25,691,691 Effective search space: 9043475232 Effective search space used: 9043475232 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 118 (50.1 bits)