BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0589.0001.p (293 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_yeaB [P] COG0053 Predicted Co/Zn/Cd cation transporters 326 2e-89 BS_ydbO [P] COG0053 Predicted Co/Zn/Cd cation transporters 311 6e-85 lin2333 [P] COG0053 Predicted Co/Zn/Cd cation transporters 296 2e-80 CAC0087 [P] COG0053 Predicted Co/Zn/Cd cation transporters 269 5e-72 L106965 [P] COG0053 Predicted Co/Zn/Cd cation transporters 211 1e-54 >BS_yeaB [P] COG0053 Predicted Co/Zn/Cd cation transporters Length = 192 Score = 326 bits (836), Expect = 2e-89, Method: Compositional matrix adjust. Identities = 0/277 (0%), Positives = 0/277 (0%) Query: 1 LENYSDIKQSEKGAWLSLFTYILLSAVKLLIGTASGSQALLADGLNNSTDIIASLAILAG 60 +E S + L I + T L AG Sbjct: 1 MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQPGLLLTQRSIHLRKHAG 60 Query: 61 LKISRRPPDSNHSYGHFRAETVAALVASFIMIAVGLQVLYQGVNKFIQPALETPDLIAAW 120 + A A I + + + TP + Sbjct: 61 QVAFPGGAVDDTDASAIAAALREAEEEVAIPPSAVEVIGVLPPVDSVTGYQVTPVVGIIP 120 Query: 121 TAAGCAVVMFAVYMYNIRLARKLNSNAMLAVAQDNRSDALVSIGAFTGIIGAQFGLPWLD 180 V + R + +G+ Sbjct: 121 PDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDSHRVWLSWYEQYFVWGMTAGI 180 Query: 181 PLTGIIVGLMICKTAWEIFRKATHDLTDGFDANELELMKQTVAEIQGVESIKDIKARIHG 240 Sbjct: 181 240 Query: 241 NNVLVDTTVCVDSNLNVVQSHDITEEIEDQLKDRHQV 277 Sbjct: 241 277 >BS_ydbO [P] COG0053 Predicted Co/Zn/Cd cation transporters Length = 309 Score = 311 bits (798), Expect = 6e-85, Method: Compositional matrix adjust. Identities = 13/272 (4%), Positives = 44/272 (16%) Query: 1 LENYSDIKQSEKGAWLSLFTYILLSAVKLLIGTASGSQALLADGLNNSTDIIASLAILAG 60 + +Q + A + + G A + A Sbjct: 1 MTQEERFEQRIAQETAIEPQDWMPDAYRKTLIRQIGQHAHSEIVGMLPEGNWITRAPTLR 60 Query: 61 LKISRRPPDSNHSYGHFRAETVAALVASFIMIAVGLQVLYQGVNKFIQPALETPDLIAAW 120 K + + + A + + + I Sbjct: 61 RKAILLAKVQDEAGHGLYLYSAAETLGCAREDIYQKMLDGRMKYSSIFNYPTLSWADIGV 120 Query: 121 TAAGCAVVMFAVYMYNIRLARKLNSNAMLAVAQDNRSDALVSIGAFTGIIGAQFGLPWLD 180 + R + + AM+ + ++ A + + Sbjct: 121 IGWLVDGAAIVNQVALCRTSYGPYARAMVKICKEESFHQRQGFEACMALAQGSEAQKQML 180 Query: 181 PLTGIIVGLMICKTAWEIFRKATHDLTDGFDANELELMKQTVAEIQGVESIKDIKARIHG 240 + + + + + + Sbjct: 181 QDAINRFWWPALMMFGPNDDNSPNSARSLTWKIKRFTNDELRQRFVDNTVPQVEMLGMTV 240 Query: 241 NNVLVDTTVCVDSNLNVVQSHDITEEIEDQLK 272 + + E+ + Sbjct: 241 PDPDLHFDTESGHYRFGEIDWQEFNEVINGRG 272 >lin2333 [P] COG0053 Predicted Co/Zn/Cd cation transporters Length = 289 Score = 296 bits (759), Expect = 2e-80, Method: Compositional matrix adjust. Identities = 137/276 (49%), Positives = 200/276 (72%) Query: 1 LENYSDIKQSEKGAWLSLFTYILLSAVKLLIGTASGSQALLADGLNNSTDIIASLAILAG 60 ++ Y+D+K++EK A+LS+F Y+ LS +K+ G S ALLADGLNN+TDI+AS+A+L G Sbjct: 1 MDGYNDLKKAEKAAFLSIFAYLFLSVLKIAAGHFGNSDALLADGLNNTTDIVASVALLIG 60 Query: 61 LKISRRPPDSNHSYGHFRAETVAALVASFIMIAVGLQVLYQGVNKFIQPALETPDLIAAW 120 L+ISR PPD++HSYGH R ET+++L+AS IM VG+QV++ + I+ P ++ A Sbjct: 61 LRISRIPPDADHSYGHRRTETISSLIASIIMFLVGVQVIWSSIVHIIEKEFTAPSMLTAI 120 Query: 121 TAAGCAVVMFAVYMYNIRLARKLNSNAMLAVAQDNRSDALVSIGAFTGIIGAQFGLPWLD 180 A V M+A+Y+YN RLA+KL+S A+ A A DNRSDA VS GAF GIIGA G+PWLD Sbjct: 121 VALFSGVFMYAIYLYNHRLAKKLDSQAVRAAAYDNRSDAFVSFGAFIGIIGAVLGVPWLD 180 Query: 181 PLTGIIVGLMICKTAWEIFRKATHDLTDGFDANELELMKQTVAEIQGVESIKDIKARIHG 240 +T +VG++I TA +IF A H LTDGFD ++LE + +A + V+ + DIKAR++G Sbjct: 181 SVTAFLVGVLIVYTAVKIFYDAAHTLTDGFDVSKLETIHDLIASVPDVKKVIDIKARMNG 240 Query: 241 NNVLVDTTVCVDSNLNVVQSHDITEEIEDQLKDRHQ 276 N + +D T+ VD +LNVV+SH+ITE +E ++++ ++ Sbjct: 241 NRIWIDATIAVDPDLNVVKSHEITEIVEQKIRNEYE 276 >CAC0087 [P] COG0053 Predicted Co/Zn/Cd cation transporters Length = 292 Score = 269 bits (687), Expect = 5e-72, Method: Compositional matrix adjust. Identities = 129/276 (46%), Positives = 197/276 (71%) Query: 2 ENYSDIKQSEKGAWLSLFTYILLSAVKLLIGTASGSQALLADGLNNSTDIIASLAILAGL 61 +NY ++K +E+G +SL Y++LS K +G S+AL ADG+N+STDIIA++AI+ GL Sbjct: 3 DNYKNLKIAERGVRISLIIYVVLSIFKFFMGYFFKSEALKADGINSSTDIIAAIAIIVGL 62 Query: 62 KISRRPPDSNHSYGHFRAETVAALVASFIMIAVGLQVLYQGVNKFIQPALETPDLIAAWT 121 KI+R+P D H+YGH RAET+++L+AS IMIAVG+ V+Y GV I E PD+ AA Sbjct: 63 KIARKPADKEHTYGHLRAETISSLLASLIMIAVGVDVVYNGVKNTIFLRNEKPDIKAAIV 122 Query: 122 AAGCAVVMFAVYMYNIRLARKLNSNAMLAVAQDNRSDALVSIGAFTGIIGAQFGLPWLDP 181 + CA V++ +Y YN +A K++S+A++ ++DN SD++V G+ GI +QFG PW+D Sbjct: 123 SLICASVVYTLYRYNRSVADKIHSSALMVSSKDNLSDSMVGFGSAFGIFASQFGFPWMDS 182 Query: 182 LTGIIVGLMICKTAWEIFRKATHDLTDGFDANELELMKQTVAEIQGVESIKDIKARIHGN 241 +T ++VG++ICKT + IFR+A ++LTD FD +LE + + + +I GV IKDI+AR+HGN Sbjct: 183 ITAVLVGIIICKTGFGIFREAVNNLTDAFDPEKLENITKIIKDIDGVSDIKDIRARVHGN 242 Query: 242 NVLVDTTVCVDSNLNVVQSHDITEEIEDQLKDRHQV 277 ++L+D + VD L+V +SH+ITE +E LK+ + Sbjct: 243 SILLDAIILVDPGLSVRESHEITEAVEKILKEEFNI 278 >L106965 [P] COG0053 Predicted Co/Zn/Cd cation transporters Length = 425 Score = 211 bits (537), Expect = 1e-54, Method: Compositional matrix adjust. Identities = 106/273 (38%), Positives = 169/273 (61%) Query: 5 SDIKQSEKGAWLSLFTYILLSAVKLLIGTASGSQALLADGLNNSTDIIASLAILAGLKIS 64 +++K +EKG W+S+ YI+LS ++ S AL A+G NN TDI+ ++AIL GL+++ Sbjct: 7 NNLKLAEKGVWVSIIAYIVLSIGQIAFAAIVHSSALQANGFNNVTDIMGNIAILIGLRLA 66 Query: 65 RRPPDSNHSYGHFRAETVAALVASFIMIAVGLQVLYQGVNKFIQPALETPDLIAAWTAAG 124 R P DS+H YGH++ E++A+L++SFIM VG +VL + V + E D + A Sbjct: 67 RIPADSDHVYGHWKIESIASLISSFIMFFVGFEVLRETVMTLLSGKEEAIDPLGALVGLF 126 Query: 125 CAVVMFAVYMYNIRLARKLNSNAMLAVAQDNRSDALVSIGAFTGIIGAQFGLPWLDPLTG 184 A VM VY+YN LA+K NS A+ A ++DN SDA+ SIG I+ + G WLD Sbjct: 127 SAFVMLGVYLYNRALAKKTNSQALSAASKDNLSDAVTSIGTVVAILASSVGWEWLDTAMA 186 Query: 185 IIVGLMICKTAWEIFRKATHDLTDGFDANELELMKQTVAEIQGVESIKDIKARIHGNNVL 244 +++ I KTA++IF ++ L+DGFD N +E ++ + + V+ +K ++ R +G+N+ Sbjct: 187 VVICGFILKTAYDIFHESVFSLSDGFDENLVEEYREAICLVPKVKGVKLVRGRSYGSNIF 246 Query: 245 VDTTVCVDSNLNVVQSHDITEEIEDQLKDRHQV 277 +D V + +L+V +SH+ TE IE L D + V Sbjct: 247 LDVVVEMSPDLSVFESHEATEFIEKMLIDNYGV 279 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.321 0.135 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 19,653,810 Number of extensions: 758160 Number of successful extensions: 1972 Number of sequences better than 1.0e-05: 38 Number of HSP's gapped: 1929 Number of HSP's successfully gapped: 38 Length of query: 293 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 187 Effective length of database: 26,153,027 Effective search space: 4890616049 Effective search space used: 4890616049 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 115 (48.9 bits)