BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0587.0018.p (443 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_ppsA_2 [Q] COG1020 Non-ribosomal peptide synthetase modules a... 283 4e-76 SA0173_2 [Q] COG1020 Non-ribosomal peptide synthetase modules an... 200 3e-51 PA2399_1 [Q] COG1020 Non-ribosomal peptide synthetase modules an... 197 2e-50 all2644_1 [Q] COG1020 Non-ribosomal peptide synthetase modules a... 194 2e-49 RSp0641_6 [Q] COG1020 Non-ribosomal peptide synthetase modules a... 189 8e-48 >BS_ppsA_2 [Q] COG1020 Non-ribosomal peptide synthetase modules and related proteins Length = 792 Score = 283 bits (724), Expect = 4e-76, Method: Compositional matrix adjust. Identities = 33/434 (7%), Positives = 81/434 (18%), Gaps = 18/434 (4%) Query: 8 KQETYYPASPAQRSIFMADKLSGRGTAYNVPVIRRIKGMLDAGRLEEALKKLVNRHESLR 67 + P + M D G ++ + + ++ L Sbjct: 3 NNGSS-PLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVSVPNGFATTADAFNQFLD 61 Query: 68 TTIELDADSGMLRQKINPAVSFAFPVYELSETETVLLIQDFVRPFRLDKPPLFRTALIRL 127 + L K + + ++ + + + Sbjct: 62 QSGVNQR-IYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQPELENAIREAYAQLSADDE 120 Query: 128 TDREETVLIIDMHHIITDGTSVSILLKDLAALYENRELEVLKLQYKDYAVRQSLLAGTEQ 187 V + D + L + L +K + +++ Q Sbjct: 121 NA-SFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAVKHVFASLFNDRAISYRVHQ 179 Query: 188 WNRQEEYWLGVFHEPVPAL--PWDGRGTEHR--AGEDPSRTAYFAIDEQTACALHRLAQT 243 L + + G +G D I + Sbjct: 180 GYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV----VFITSAWGLGEMVVQGA 235 Query: 244 TESTLFMVLMAAFTALLSKLSDCEDIVVGMPTSGRHHEGLDNIVGMLVNTLAVRFFACRK 303 F V A + + + V + R Sbjct: 236 VNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHGKQVKIEDVPQEQRDIFSLT 295 Query: 304 LSFQEYLYQVKDKLIEAYENQDYPLDRLVEKLNGSHNLQGRPLFNVMFQFAEMEAAEQID 363 + L + ++ + Y +Q RP Sbjct: 296 NEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQARPETVRSRGQVMER-YTLHS 354 Query: 364 ----AIDMVSFGHDSVPGKFDLHFIVVQESSSLGIEITYFESLFSEEAIGELISGFIEII 419 + + GH G + I + + ++ ++ I+ Sbjct: 355 QGKIIAEGRAIGHRI--GAGPVKVIHDISEMNRIEPGDVLVTDMTDPDWEPIMKKASAIV 412 Query: 420 QVITSNLETGLGDL 433 Sbjct: 413 TNRGGRTCHAAIIA 426 Score = 60.5 bits (145), Expect = 5e-09, Method: Compositional matrix adjust. Identities = 0/288 (0%), Positives = 0/288 (0%), Gaps = 35/288 (12%) Query: 16 SPAQRSIFMADKLSGRGTAYNVPVIRRIKGMLDAGRLEEALKKLVNRHESLRTTIELDAD 75 Sbjct: 1051 -- 1108 Query: 76 SGMLR--QKINPAVSFAFPVY----------------ELSETETVLLIQDFVRPFRLDKP 117 Sbjct: 1109 ----- 1163 Query: 118 PLFRTALIRLTDREETVLIIDMHHIITDGTSVSILLKDLAALYENRELEVLKLQ------ 171 Sbjct: 1164 --- 1220 Query: 172 -YKDYAVRQSLLAGTEQWNRQEEYWLGVFHEPVPALPWDGRGTEHRAGEDPSRTAYFAID 230 Sbjct: 1221 1280 Query: 231 EQTACALHRLAQTTESTLFMVLMAAFTALLSKLSDCEDIVVGMPTSGR 278 Sbjct: 1281 1328 >SA0173_2 [Q] COG1020 Non-ribosomal peptide synthetase modules and related proteins Length = 346 Score = 200 bits (509), Expect = 3e-51, Method: Compositional matrix adjust. Identities = 0/424 (0%), Positives = 0/424 (0%), Gaps = 23/424 (5%) Query: 13 YPASPAQRSIFMADKLSGRGTAYNVPVIRRIKGMLDAGRLEEALKKLVNRHESLRTTIEL 72 + + G + A + + T L Sbjct: 5 RIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGL 64 Query: 73 DADSGMLRQKINPAVSFAFPVYELSETETVLLIQDFVRPFRLDKPPLFRTALIRLTDREE 132 + + S + + +RP + R AL + Sbjct: 65 TETY--VGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHD--SLKR 120 Query: 133 TVLIIDMHHIITDGTSVSILLKDLAALYENRELEVLKLQYKDYAVRQSLLAGTEQWNRQE 192 + + + L + + V L D R Sbjct: 121 LQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKI----RYI 176 Query: 193 EYWLGVFHEPVPALPWDGRGTEHRAGEDPSRTAYFAIDEQTACALHRLAQTTESTLFMVL 252 + L + R + + + A + E + L Sbjct: 177 GVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCL 236 Query: 253 MAAFTALLSKLSDCEDIVVGMPTSGRHHEGLDNIVGMLVNTLAVRFFACRKLSFQEYLYQ 312 S + + + + + + Sbjct: 237 GFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFV 296 Query: 313 VKDKLIEAYENQDYPLDRLVEKLNGSHNLQGRPLFNVMFQFAEMEAAEQIDAIDMVSFGH 372 + + + +D+L + H + + ++ Sbjct: 297 RRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEIEAVHQVYT------- 349 Query: 373 D--------SVPGKFDLHFIVVQESSSLGIEITYFESLFSEEAIGELISGFIEIIQVITS 424 Sbjct: 350 409 Query: 425 NLET 428 Sbjct: 410 413 >PA2399_1 [Q] COG1020 Non-ribosomal peptide synthetase modules and related proteins Length = 346 Score = 197 bits (502), Expect = 2e-50, Method: Compositional matrix adjust. Identities = 0/437 (0%), Positives = 0/437 (0%), Gaps = 27/437 (6%) Query: 16 SPAQRSIFMADKLSGRGTAYNVPVIRRIKGMLDAGRLEEALKKLVNRHESLRTTIELDAD 75 A+ + T V G + + + ++ + Sbjct: 48 DVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDR 107 Query: 76 SGMLRQKINPAVSFAFPVYELSETETVLLIQD----FVRPFRLDKPPLFRTALIRLTDRE 131 ++ L + + + + L L Sbjct: 108 KN--IREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDA-L 164 Query: 132 ETVLIIDMHHIITDGTSVSILLKDLAALYE--NRELEV----LKLQYKDYAVRQSLLAGT 185 I + + L + + V Sbjct: 165 AEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQ 224 Query: 186 EQWNRQEEYWLGVF--HEPVPALPWDGRGTEHRAGEDPSRTAYF--AIDEQTACALHRLA 241 + Y F A Q A A + Sbjct: 225 YEGVELLAYSCLGFGTLTGKYLNGAK----PAGARNTLFSRFTRYSGEQTQKAVAAYVDI 280 Query: 242 QTTESTLFMVLMAAFTALLSKLSDCEDIVVGMPTSGRHHEGLDNIVGMLVNTLAVRFFAC 301 + AF ++ M + E L + V Sbjct: 281 ARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEIEAVHQV 340 Query: 302 RKLSFQEYLYQVKDKLIEAYENQDYPLDRLVEKLNGSHNLQGRPLFNVMFQFAEMEA--- 358 Sbjct: 341 - 399 Query: 359 --AEQIDAIDMVSFGHDSVPGKFDLHFIVVQESSSLGIEITYFESLFSEEAIGELISGFI 416 Sbjct: 400 459 Query: 417 EIIQVITSNLETGLGDL 433 Sbjct: 460 476 >all2644_1 [Q] COG1020 Non-ribosomal peptide synthetase modules and related proteins Length = 346 Score = 194 bits (494), Expect = 2e-49, Method: Compositional matrix adjust. Identities = 0/439 (0%), Positives = 0/439 (0%), Gaps = 34/439 (7%) Query: 6 ERKQETYYPASPAQRSIFMADKLSGRGTAYNVPVIRRIKGMLDAGRLEEALKKLVNRHES 65 S A + ++ +V + + + L E Sbjct: 18 LGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTETYVGNWLAKHG 77 Query: 66 LRTTIELDADSGMLRQKINPAVSFAFPVYELSE-----TETVLLIQDFV---RPFRLDKP 117 R + + + N + L Sbjct: 78 SREKLIIASKVSG--PSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYLDLYQVHWPQ 135 Query: 118 PLFRTALIRLTDREETVLIIDMHHIITDGTSVSILLKDLAALYENRELEVLKLQYKDYAV 177 D + + + + E ++Y A Sbjct: 136 RPTNCFGKLGYSWT-DSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGVMRYLHLAD 194 Query: 178 RQSLLAGTEQWNRQEEYWLGVFHEPVPALPWDGRGTEHRAGEDPSRTA---------YFA 228 + L N L + Sbjct: 195 KHDLPRIVTIQNPYSLLN----RSFEVGLA-----EVSQYEGVELLAYSCLGFGTLTGKY 245 Query: 229 IDEQTACALHRLAQTTESTLFMVLMAAFTALLSKLSDCEDIVVGMPTSGRHHEGLDNIVG 288 ++ + + A ++ + Sbjct: 246 LNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQPFVAST 305 Query: 289 MLVNTLAVRFFACRKLSFQEYLYQVKDKLIEAYENQDYPLDRLVEKLNGSHNLQGRPLFN 348 Sbjct: 306 365 Query: 349 VMFQFAEM-EAAEQIDAIDMVSFGHDSVPGKFDLHFIVVQESSSLGIEITYFESLFSEEA 407 Sbjct: 366 425 Query: 408 IGELISGFIEIIQVITSNL 426 Sbjct: 426 ---- 440 >RSp0641_6 [Q] COG1020 Non-ribosomal peptide synthetase modules and related proteins Length = 346 Score = 189 bits (480), Expect = 8e-48, Method: Compositional matrix adjust. Identities = 0/412 (0%), Positives = 0/412 (0%), Gaps = 34/412 (8%) Query: 14 PASPAQRSIFMADKLSGRGTAYNVPVIRRIKGMLDAGRLEEALKKLVNRHESLRTTIELD 73 + + G + A + + T L Sbjct: 6 IPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLT 65 Query: 74 ADSGMLRQKINPA-VSFAFPVYELS------ETETVLLIQDFVRPFRLDKPPLFRTALIR 126 + + + + + Q R + L Sbjct: 66 --ETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQT 123 Query: 127 LTDREETVLIIDMHHIITDGTSVSILLKDLAALYE------NRELEVLKLQYKDYAVRQS 180 + G A+ E + ++ Sbjct: 124 DYLDLY-QVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNET 182 Query: 181 LLAGTEQWNRQEEYW-LGVFHEP-VPALPWDGRGTEHRAGEDPSRTAY------FAIDEQ 232 + +++ + +L + + Sbjct: 183 AFGVMRYLHLADKHDLPRIVTIQNPYSLLNR------SF--EVGLAEVSQYEGVELLAYS 234 Query: 233 TACALHRLAQTTESTLFMVLMAAFTALLSKLSDCEDIVVGMPTSGRHHEGLDNIVGMLVN 292 + + ++ S + + M + Sbjct: 235 CLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALA 294 Query: 293 TLAVRFFACRKLSFQEYLYQVKDKLIEAYENQDYPLDRLVEKLNGSHNLQGRPLFNVMFQ 352 + + Sbjct: 295 FVRRQ- 353 Query: 353 FAEMEAAE-QIDAIDMVSFGHDSVPGKFDLHFIVVQESSSLGIEITYFESLF 403 Sbjct: 354 405 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.136 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 30,975,232 Number of extensions: 1334027 Number of successful extensions: 3841 Number of sequences better than 1.0e-05: 40 Number of HSP's gapped: 3732 Number of HSP's successfully gapped: 44 Length of query: 443 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 333 Effective length of database: 25,691,691 Effective search space: 8555333103 Effective search space used: 8555333103 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 117 (49.7 bits)