BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0587.0003.p (335 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_msmR [K] COG1609 Transcriptional regulators 210 2e-54 SP1854 [K] COG1609 Transcriptional regulators 185 1e-46 BS_lacR [K] COG1609 Transcriptional regulators 180 3e-45 ZebgR [K] COG1609 Transcriptional regulators 177 2e-44 ebgR [K] COG1609 Transcriptional regulators 177 2e-44 >BS_msmR [K] COG1609 Transcriptional regulators Length = 344 Score = 210 bits (535), Expect = 2e-54, Method: Compositional matrix adjust. Identities = 129/338 (38%), Positives = 184/338 (54%), Gaps = 13/338 (3%) Query: 7 IKDIAREAGVSAATVSRVLNNDLSLAVSEVTRTRVFAVAEQLGYKPARLKQLKRDTERSG 66 IKDIA +A VS+ATVSR+LN D SL+V+ TR RV +AE+LGY+ ++ R ++ Sbjct: 4 IKDIALKAKVSSATVSRILNEDESLSVAGETRQRVINIAEELGYQTVAKRRKSRGQKQRA 63 Query: 67 KTVSLLLRC-SVEEERDDPYYGSIRRGIELRAEELGIXXXXLGQTLRGHGQLPALRACDG 125 + + +L C S ++ER DPY+ SIR+GIE E I G Q R DG Sbjct: 64 QPLIGVLSCLSPDQERQDPYFSSIRKGIEKECFEQEIFIT--NSIHLGSFQEHIFRELDG 121 Query: 126 LIVVGGFDPRELESLHADPSTIVLVDQYGEQTDYDSVRTHFRQAVEQALGHLMALGHRDI 185 +IV+G ++ + V ++ + YDS+ F A QA+ HL LG++ + Sbjct: 122 VIVIGRVHDEAVKHISGRLEHAVFINHSPDPQAYDSIGIDFESASRQAIDHLFDLGYKRL 181 Query: 186 AFIGG--------GGDGERRAHHFERI--MLERGWFDDRLVRTGGWSSADGYRMMNELLA 235 +IGG G RR +R+ LE V G +S +GYR+M + + Sbjct: 182 GYIGGQEKEHTLKDGQSIRRTIEDKRLTAFLESAAPQPEHVLIGEYSMREGYRLMKKAID 241 Query: 236 GAKRPTACFAASDPLAVGALRALHGQGVRVPEEMAIVGFDDIEMAAYVQPPLTTVRAYPE 295 P A F ASD +A+GAL+AL G++VP + AIV F+ IE A + PLTTV+ Y E Sbjct: 242 QGHLPEAFFIASDSMAIGALKALQEAGLQVPRDTAIVSFNGIEEAEFASTPLTTVKVYTE 301 Query: 296 QMGKAAVQLLVERFEGREAPSHTITGTKLIVRESCGGT 333 +MG+ V+LL++R GR P H T LIVR+SCG T Sbjct: 302 EMGRTGVKLLLDRLNGRTLPQHVTLPTTLIVRQSCGCT 339 >SP1854 [K] COG1609 Transcriptional regulators Length = 335 Score = 185 bits (469), Expect = 1e-46, Method: Compositional matrix adjust. Identities = 119/341 (34%), Positives = 186/341 (54%), Gaps = 24/341 (7%) Query: 4 LPTIKDIAREAGVSAATVSRVLNNDLSLAVSEVTRTRVFAVAEQLGYKPARLKQLKR-DT 62 + T+KDIA+ A VS ATVSRVLN D SL+V+E TR R+ VAE+LGY K LK ++ Sbjct: 1 MATLKDIAQLASVSIATVSRVLNRDQSLSVTEETRHRILTVAEELGYT----KHLKTGES 56 Query: 63 ERSGKTVSLLLRCSVEEERDDPYYGSIRRGIELRAEELGIXXXXLGQTLRGHGQLPALRA 122 + + ++++ S + E DD YY IR GIE RA+EL LR P + Sbjct: 57 HKPKQKIAIIQWVSEQGELDDLYYYQIRLGIEKRAQELDY------DILRYFNDHPFTLS 110 Query: 123 CD--GLIVVGGFDPRELESLHADPSTIVLVDQYGEQTDYDSVRTHFRQAVEQALGHLMAL 180 + G++ +G F ++ + +V +D + + T F A++Q + + ++ Sbjct: 111 EEVIGILCIGKFSRAQISAFEEYQKPLVFLDSDTLSLGHTCIITDFYTAMKQVVDYFLSQ 170 Query: 181 GHRDIAFIGG---GGDGE-----RRAHHFERIMLERGWFDDRLVRTGGWSSADGYRMMNE 232 G I + G D E +R +F+ RG + D LV G +++ GY +M E Sbjct: 171 GMDRIGILTGLEETTDQEEIIQDKRLENFKNYSQARGIYHDELVFQGRFTAQSGYDLMKE 230 Query: 233 LLA--GAKRPTACFAASDPLAVGALRALHGQGVRVPEEMAIVGFDDIEMAAYVQPPLTTV 290 + G + P A FAASD LA+GALRAL G+ +P+ ++++ F+D + V PPL+++ Sbjct: 231 AIQSLGDQLPPAFFAASDSLAIGALRALQEAGISLPDRVSLISFNDTSLTKQVYPPLSSI 290 Query: 291 RAYPEQMGKAAVQLL-VERFEGREAPSHTITGTKLIVRESC 330 Y E+MG+A + +L E GR+ PS T+ GT+L +RES Sbjct: 291 TVYTEEMGRAGMDILNKEVLHGRKIPSLTMLGTRLTLREST 331 >BS_lacR [K] COG1609 Transcriptional regulators Length = 330 Score = 180 bits (456), Expect = 3e-45, Method: Compositional matrix adjust. Identities = 115/340 (33%), Positives = 178/340 (52%), Gaps = 22/340 (6%) Query: 4 LPTIKDIAREAGVSAATVSRVLNNDLSLAVSEVTRTRVFAVAEQLGYKPARLKQLKRDTE 63 + TIKDIA+EAG S +TVSRVLNND SL+V + TR +++ AE+L Y+ ++ L Sbjct: 1 MATIKDIAQEAGFSISTVSRVLNNDESLSVPDETREKIYEAAEKLNYRKKTVRPL----- 55 Query: 64 RSGKTVSLLLRCSVEEERDDPYYGSIRRGIELRAEELGIXXXXLGQTLRGHGQLPALRAC 123 K ++ L + +EE +D Y+ ++R +E A+ + + G +P Sbjct: 56 --VKHIAFLYWLTDKEELEDVYFKTMRLEVEKLAKAFNVDMTTY-KIADGIESIP--EHT 110 Query: 124 DGLIVVGGFDPRELESLHADPSTIVLVDQYGEQTDYDSVRTHFRQAVEQALGHLMALGHR 183 +G I VG F EL L V +D + +DSVR Q + + L GH+ Sbjct: 111 EGFIAVGTFSDEELAFLRNLTENGVFIDSTPDPDHFDSVRPDLAQMTRKTVNILTEKGHK 170 Query: 184 DIAFIGGG--------GDGERRAHHFERIMLERGWFDDRLVRTG-GWSSADGYRMMNELL 234 I FIGG + + R F M E+ D+R + G+S +GYR+M+ + Sbjct: 171 SIGFIGGTYKNPNTNQDEMDIREQTFRSYMREKAMLDERYIFCHRGFSVENGYRLMSAAI 230 Query: 235 --AGAKRPTACFAASDPLAVGALRALHGQGVRVPEEMAIVGFDDIEMAAYVQPPLTTVRA 292 G + PTA A+DP+AVG L+AL+ +G+ +P ++IV ++I A YV PPLTT Sbjct: 231 DTLGDQLPTAFMIAADPIAVGCLQALNEKGIAIPNRVSIVSINNISFAKYVSPPLTTFHI 290 Query: 293 YPEQMGKAAVQLLVERFEGREAPSHTI-TGTKLIVRESCG 331 ++ K AVQLL+E+ + + T+ G +LIVR+S Sbjct: 291 DIHELCKNAVQLLLEQVQDKRRTVKTLYVGAELIVRKSMN 330 >ZebgR [K] COG1609 Transcriptional regulators Length = 327 Score = 177 bits (449), Expect = 2e-44, Method: Compositional matrix adjust. Identities = 110/328 (33%), Positives = 174/328 (53%), Gaps = 9/328 (2%) Query: 4 LPTIKDIAREAGVSAATVSRVLNNDLSLAVSEVTRTRVFAVAEQLGYKPARLKQLKRDTE 63 + T+KDIA EAGVS ATVSRVLN+D +L V E T+ R+ +AE+L YK + ++L+ Sbjct: 1 MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEKLEYKTSSARKLQTGAV 60 Query: 64 RSGKTVSLLLRCSVEEERDDPYYGSIRRGIELRAEELGIXXXXLGQTLRGHGQLPALRAC 123 +++ E E +DPYY +IR GIE + E+L I + H LP ++ Sbjct: 61 NQHHILAIY-SYQQELEINDPYYLAIRHGIETQCEKLAIELTNCYE----HSGLPDIKNV 115 Query: 124 DGLIVVGGFDPRELESLHADPSTIVLVDQYGEQTDYDSVRTHFRQAVEQALGHLMALGHR 183 G+++VG P + A I +D + + YD+V + ++ + + G Sbjct: 116 TGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLARISKEIIDFYINQGVN 175 Query: 184 DIAFIGG---GGDGERRAHHFERIMLERGWFDDRLVRTGGWSSADGYRMMNELLAGAKRP 240 I FIGG G + R F + + + GG+SS+ GY + ++LA P Sbjct: 176 RIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSSSSGYELAKQMLAREDYP 235 Query: 241 TACFAASDPLAVGALRALHGQGVRVPEEMAIVGFDDIEMAAYVQPPLTTVRAYPEQMGKA 300 A F ASD +A+G LRA+H +G+ +P++++++ DDI A + PPL+TVR + E MG Sbjct: 236 KALFVASDSIAIGVLRAIHERGLNIPQDISLISVDDIPTARFTFPPLSTVRIHSEMMGSQ 295 Query: 301 AVQLLVERF-EGREAPSHTITGTKLIVR 327 V L+ E+ +GR P +KL +R Sbjct: 296 GVNLVYEKARDGRALPLLVFVPSKLKLR 323 >ebgR [K] COG1609 Transcriptional regulators Length = 327 Score = 177 bits (449), Expect = 2e-44, Method: Compositional matrix adjust. Identities = 110/328 (33%), Positives = 175/328 (53%), Gaps = 9/328 (2%) Query: 4 LPTIKDIAREAGVSAATVSRVLNNDLSLAVSEVTRTRVFAVAEQLGYKPARLKQLKRDTE 63 + T+KDIA EAGVS ATVSRVLN+D +L V E T+ R+ +AE+L YK + ++L+ Sbjct: 1 MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEKLEYKTSSARKLQTGAV 60 Query: 64 RSGKTVSLLLRCSVEEERDDPYYGSIRRGIELRAEELGIXXXXLGQTLRGHGQLPALRAC 123 +++ E E +DPYY +IR GIE + E+LGI + H LP ++ Sbjct: 61 NQHHILAIY-SYQQELEINDPYYLAIRHGIETQCEKLGIELTNCYE----HSGLPDIKNV 115 Query: 124 DGLIVVGGFDPRELESLHADPSTIVLVDQYGEQTDYDSVRTHFRQAVEQALGHLMALGHR 183 G+++VG P + A I +D + + YD+V + ++ + + G Sbjct: 116 TGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLARISKEIIDFYINQGVN 175 Query: 184 DIAFIGG---GGDGERRAHHFERIMLERGWFDDRLVRTGGWSSADGYRMMNELLAGAKRP 240 I FIGG G + R F + + + GG+SS+ GY + ++LA P Sbjct: 176 RIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSSSSGYELAKQMLAREDYP 235 Query: 241 TACFAASDPLAVGALRALHGQGVRVPEEMAIVGFDDIEMAAYVQPPLTTVRAYPEQMGKA 300 A F ASD +A+G LRA+H +G+ +P++++++ +DI A + PPL+TVR + E MG Sbjct: 236 KALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTARFTFPPLSTVRIHSEMMGSQ 295 Query: 301 AVQLLVERF-EGREAPSHTITGTKLIVR 327 V L+ E+ +GR P +KL +R Sbjct: 296 GVNLVYEKARDGRALPLLVFVPSKLKLR 323 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.137 0.400 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 25,042,338 Number of extensions: 996530 Number of successful extensions: 2576 Number of sequences better than 1.0e-05: 151 Number of HSP's gapped: 2362 Number of HSP's successfully gapped: 152 Length of query: 335 Length of database: 38,378,431 Length adjustment: 107 Effective length of query: 228 Effective length of database: 26,037,693 Effective search space: 5936594004 Effective search space used: 5936594004 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 116 (49.3 bits)