BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0586.0066.p (141 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value YPO1799 [N] COG1815 Flagellar basal body protein 49 3e-06 STM1364 [R] COG0628 Predicted permease 47 8e-06 >YPO1799 [N] COG1815 Flagellar basal body protein Length = 137 Score = 48.5 bits (114), Expect = 3e-06, Method: Compositional matrix adjust. Identities = 40/140 (28%), Positives = 66/140 (47%), Gaps = 24/140 (17%) Query: 12 SVSFQRLQGGLDAATKRQSVLANNVANADTPNFKRSDVNFESYL-----QQQENGLKSTL 66 ++ FQ Q L+ +RQ +L++N+ANADTP F+ D++F S L Q + NG +L Sbjct: 8 ALRFQ--QEALNLRAQRQEILSSNIANADTPGFQARDIDFSSQLKKVMEQGRANGTGVSL 65 Query: 67 NAKVTDSRHFQFGTGTIVPTATVSTDE-------TTSXXXXXXXXXXXREQALSAENQLR 119 + +T +RH +P TV + RE+ A+N L+ Sbjct: 66 S--LTSARH--------IPATTVQPPDLDLLFRIPDQPAMDGNTVDMDRERTNFADNSLK 115 Query: 120 YNSYVAQLNSQITMMRTVVE 139 Y + + L QI M +V++ Sbjct: 116 YQTDLTILGGQIKGMMSVLQ 135 >STM1364 [R] COG0628 Predicted permease Length = 372 Score = 47.0 bits (110), Expect = 8e-06, Method: Compositional matrix adjust. Identities = 5/107 (4%), Positives = 14/107 (13%), Gaps = 6/107 (5%) Query: 14 SFQRLQGGLDAATKRQSVLANNVANADTPNFKRSDVNFESYLQQQENGLKSTLNAKVTDS 73 R + + +++ P + LK Sbjct: 5 RQPRDIAQVLLSVLFLAIMIVACLWIVQPFILGFAWAGTIVIATWPVLLKLQ----KILW 60 Query: 74 RHFQFGTGTIVPTATVSTDETTSXXXXXXXXXXXREQALSAENQLRY 120 + + + A Sbjct: 61 GRRSLAVLVMTLLLVL--LFVIPVALLVNSIVDGSGPLIHAVTGGDM 105 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.311 0.125 0.335 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 8,135,343 Number of extensions: 228904 Number of successful extensions: 317 Number of sequences better than 1.0e-05: 2 Number of HSP's gapped: 316 Number of HSP's successfully gapped: 2 Length of query: 141 Length of database: 38,378,431 Length adjustment: 96 Effective length of query: 45 Effective length of database: 27,306,367 Effective search space: 1228786515 Effective search space used: 1228786515 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 110 (47.0 bits)