BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0586.0060.p (437 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CT669 [NU] COG1157 Flagellar biosynthesis/type III secretory pat... 451 e-126 CPn0707 [NU] COG1157 Flagellar biosynthesis/type III secretory p... 447 e-125 PA1697 [NU] COG1157 Flagellar biosynthesis/type III secretory pa... 429 e-120 YPCD1.40 [NU] COG1157 Flagellar biosynthesis/type III secretory ... 427 e-119 PA1104 [NU] COG1157 Flagellar biosynthesis/type III secretory pa... 404 e-112 >CT669 [NU] COG1157 Flagellar biosynthesis/type III secretory pathway ATPase Length = 442 Score = 451 bits (1160), Expect = e-126, Method: Compositional matrix adjust. Identities = 230/421 (54%), Positives = 303/421 (71%), Gaps = 11/421 (2%) Query: 21 GKVTQVIGLMVESEGPDASIGDVCYIYPAKGNKPLQAEVVGFRDNKVLLMPLGELQAIGP 80 G++ +V+G+++++ PD +G+VC + G +PL EVVGF N V L PLGEL + P Sbjct: 25 GRIIEVVGMLIKAVVPDVRVGEVCLV-KRHGMEPLVTEVVGFTQNFVFLSPLGELTGVSP 83 Query: 81 GCDVVGTGKPLSVQVGSELLGKVLDGLGQPLD----GSL--IPARMPHSSTFNIPSNPLN 134 +V+ TG PL ++ G LLG+VL+GLG P+D G L + A P F P +PL+ Sbjct: 84 SSEVMATGLPLHIRAGEGLLGRVLNGLGNPIDTETKGPLENVDAIYP---IFKAPPDPLH 140 Query: 135 RPRVQEPISIGVRAIDGLLTIGKGQRVGIFAGSGVGKSTLMGMIARNTS-ADVNVIALIG 193 R +++ +S GVR IDG+LT+ KGQR+GIFAG+GVGKS+L+GMIARN AD+NVIALIG Sbjct: 141 RAKLRTILSTGVRCIDGMLTVAKGQRIGIFAGAGVGKSSLLGMIARNAEEADINVIALIG 200 Query: 194 ERGREVLDFIERDLGPEGLQRSVVVVATSDQPALIRIKGALIATTIAEYFRDRGLNVMLM 253 ERGREV +FIE DLG EG++RSV+VV+TSDQ + +R+ A + T IAEYFRD+G V+LM Sbjct: 201 ERGREVREFIENDLGEEGMKRSVIVVSTSDQSSQLRLNAAYVGTAIAEYFRDQGKTVVLM 260 Query: 254 MDSVTRYAMAQREVGLAVGEPPAMRGYTPSVFASLPKLLERAGTGPTGSITAFYTVLVDG 313 MDSVTR+A A REVGLA GEPPA GYTPSVF++LPKLLERAG G+ITAFYTVLV G Sbjct: 261 MDSVTRFARALREVGLAAGEPPARAGYTPSVFSTLPKLLERAGASDKGTITAFYTVLVAG 320 Query: 314 DDMNEPIADAVRGILDGHIVLNRNIANKGHFPAIDVLSSISRVMKDIAPEEQIAAAENVK 373 DDMNEP+AD V+ ILDGHIVL+ +A H+PAIDVL+SISR++ I PEEQ + Sbjct: 321 DDMNEPVADEVKSILDGHIVLSNALAQAYHYPAIDVLASISRLLTAIVPEEQRRIIGRAR 380 Query: 374 RLMAVYKDSEDLINIGAYQRGSNAQIDESIHYIDSIWNFTKQKVNEKVTLAEVQESLISQ 433 ++A YK +E LI IG Y+RGS+ ++D +I +ID + F KQ ++EK E + L + Sbjct: 381 EVLAKYKANEMLIRIGEYRRGSDREVDFAIDHIDKLNRFLKQDIHEKTNYEEAAQQLRAI 440 Query: 434 F 434 F Sbjct: 441 F 441 >CPn0707 [NU] COG1157 Flagellar biosynthesis/type III secretory pathway ATPase Length = 211 Score = 447 bits (1149), Expect = e-125, Method: Compositional matrix adjust. Identities = 0/432 (0%), Positives = 0/432 (0%), Gaps = 11/432 (2%) Query: 10 QLRNFDPVRINGKVTQVIGLMVESEGPDASIGDVCYIYPAKGNKPLQAEVVGFRDNKVLL 69 + N P + L+ A+ V AK + Sbjct: 14 RENNPHPTTELNFSSPFELLIAVLLSAQATDVSVNKA-TAKLYPVANTPAAMLELGVEGV 72 Query: 70 MPLGELQAIGPGCDVVGTGKPLSVQVGSELLGKVLDGLGQPLDGSLIPARMPHSST---- 125 + + + + L R + Sbjct: 73 KTYIKTIGLYNSKAENIIKTCRILLEQHNGEVPEDRAALEALP---GVGRKTANVVLNTA 129 Query: 126 --FNIPSNPLNRPRVQEPISIGVRAIDGLLTIGKGQRVGIFAGSGVGKSTLMGMIARNTS 183 + + + RV + + V ++ + Sbjct: 130 FGWPTIAVDTHIFRVCNRTQFAPGKNVEQVEEKLLKVVPAEFKVDCHHWLILHGRYTCIA 189 Query: 184 -ADVNVIALIGERGREVLDFIERDLGPEGLQRSVVVVATSDQPALIRIKGALIATTIAEY 242 Sbjct: 190 R 249 Query: 243 FRDRGLNVMLMMDSVTRYAMAQREVGLAVGEPPAMRGYTPSVFASLPKLLERAGTGPTGS 302 Sbjct: 250 309 Query: 303 ITAFYTVLVDGDDMNEPIADAVRGILDGHIVLNRNIANKGHFPAIDVLSSISRVMKDIAP 362 Sbjct: 310 369 Query: 363 EEQIAAAENVKRLMAVYKDSEDLINIGAYQRGSNAQIDESIHYIDSIWNFTKQKVNEKVT 422 Sbjct: 370 429 Query: 423 LAEVQESLISQF 434 Sbjct: 430 441 >PA1697 [NU] COG1157 Flagellar biosynthesis/type III secretory pathway ATPase Length = 440 Score = 429 bits (1102), Expect = e-120, Method: Compositional matrix adjust. Identities = 214/427 (50%), Positives = 292/427 (68%) Query: 6 RYKEQLRNFDPVRINGKVTQVIGLMVESEGPDASIGDVCYIYPAKGNKPLQAEVVGFRDN 65 R + + P++I G+VTQV G ++++ P IG++C + + L AEV+GF+ + Sbjct: 11 RMRHAIEGCRPIQIRGRVTQVTGTLLKAVVPGVRIGELCQLRNPDQSLALLAEVIGFQQH 70 Query: 66 KVLLMPLGELQAIGPGCDVVGTGKPLSVQVGSELLGKVLDGLGQPLDGSLIPARMPHSST 125 + LL PLGE+ + +V TG V VG LLG+VLDGLG+P DGS Sbjct: 71 QALLTPLGEMLGVSSNTEVSPTGGMHRVAVGEHLLGQVLDGLGRPFDGSPPAEPAAWYPV 130 Query: 126 FNIPSNPLNRPRVQEPISIGVRAIDGLLTIGKGQRVGIFAGSGVGKSTLMGMIARNTSAD 185 + P++R ++ P+S+GVRAIDGLLT G+GQR+GIFA +G GKSTL+ + RN D Sbjct: 131 YRDAPQPMSRRLIERPLSLGVRAIDGLLTCGEGQRMGIFAAAGGGKSTLLASLVRNAEVD 190 Query: 186 VNVIALIGERGREVLDFIERDLGPEGLQRSVVVVATSDQPALIRIKGALIATTIAEYFRD 245 V V+AL+GERGREV +FIE DLG +GL+RSV+VVATSD+PA+ R K +AT+IAEYFRD Sbjct: 191 VTVLALVGERGREVREFIESDLGEQGLRRSVLVVATSDRPAMERAKAGFVATSIAEYFRD 250 Query: 246 RGLNVMLMMDSVTRYAMAQREVGLAVGEPPAMRGYTPSVFASLPKLLERAGTGPTGSITA 305 +G V+L+MDS+TR+A AQRE+GLA GEPP RGY PSVFA+LP+L+ERAG GSITA Sbjct: 251 QGRRVLLLMDSLTRFARAQREIGLAAGEPPTRRGYPPSVFAALPRLMERAGQSERGSITA 310 Query: 306 FYTVLVDGDDMNEPIADAVRGILDGHIVLNRNIANKGHFPAIDVLSSISRVMKDIAPEEQ 365 YTVLV+GDDM+EP+AD R ILDGHIVL+R +A H+PAIDVL S+SRVM I ++Q Sbjct: 311 LYTVLVEGDDMSEPVADETRSILDGHIVLSRKLAAANHYPAIDVLHSVSRVMNQIVDDDQ 370 Query: 366 IAAAENVKRLMAVYKDSEDLINIGAYQRGSNAQIDESIHYIDSIWNFTKQKVNEKVTLAE 425 AA ++ +A Y++ E L+ IG YQ+G +++ D +I I +I + +Q +E A+ Sbjct: 371 RHAAGRLREWLAKYEEVELLLKIGEYQKGQDSEADRAIEKIGAIRQWLRQGTHETSDYAQ 430 Query: 426 VQESLIS 432 L S Sbjct: 431 ACAQLRS 437 >YPCD1.40 [NU] COG1157 Flagellar biosynthesis/type III secretory pathway ATPase Length = 439 Score = 427 bits (1099), Expect = e-119, Method: Compositional matrix adjust. Identities = 212/414 (51%), Positives = 288/414 (69%), Gaps = 3/414 (0%) Query: 17 VRINGKVTQVIGLMVESEGPDASIGDVCYIYPAKGNKPLQAEVVGFRDNKVLLMPLGELQ 76 ++I G+VTQV G ++++ P IG++CY+ + LQAEV+GF ++ LL+PLGE+ Sbjct: 21 IQIRGRVTQVTGTLLKAVVPGVRIGELCYLRNPDNSLSLQAEVIGFAQHQALLIPLGEMY 80 Query: 77 AIGPGCDVVGTGKPLSVQVGSELLGKVLDGLGQPLDGSLIPARMPHSSTFNIPSNPLNRP 136 I +V TG V VG LLG+VLDGLGQP DG +P + P++R Sbjct: 81 GISSNTEVSPTGTMHQVGVGEHLLGQVLDGLGQPFDGGHLPEPAAWYPVYQDAPAPMSRK 140 Query: 137 RVQEPISIGVRAIDGLLTIGKGQRVGIFAGSGVGKSTLMGMIARNTSADVNVIALIGERG 196 + P+S+G+R IDGLLT G+GQR+GIFA +G GKSTL+ + R+ DV V+ALIGERG Sbjct: 141 LITTPLSLGIRVIDGLLTCGEGQRMGIFAAAGGGKSTLLASLIRSAEVDVTVLALIGERG 200 Query: 197 REVLDFIERDLGPEGLQRSVVVVATSDQPALIRIKGALIATTIAEYFRDRGLNVMLMMDS 256 REV +FIE DLG EGL+++V+VVATSD+P++ R K +AT+IAEYFRD+G V+L+MDS Sbjct: 201 REVREFIESDLGEEGLRKAVLVVATSDRPSMERAKAGFVATSIAEYFRDQGKRVLLLMDS 260 Query: 257 VTRYAMAQREVGLAVGEPPAMRGYTPSVFASLPKLLERAGTGPTGSITAFYTVLVDGDDM 316 VTR+A AQRE+GLA GEPP RGY PSVFA+LP+L+ERAG GSITA YTVLV+GDDM Sbjct: 261 VTRFARAQREIGLAAGEPPTRRGYPPSVFAALPRLMERAGQSSKGSITALYTVLVEGDDM 320 Query: 317 NEPIADAVRGILDGHIVLNRNIANKGHFPAIDVLSSISRVMKDIAPEEQIAAAENVKRLM 376 EP+AD R ILDGHI+L+R +A H+PAIDVL S SRVM I +E A +++RL+ Sbjct: 321 TEPVADETRSILDGHIILSRKLAAANHYPAIDVLRSASRVMNQIVSKEHKTWAGDLRRLL 380 Query: 377 AVYKDSEDLINIGAYQRGSNAQIDESIHYIDSIWNFTKQKVNEKVTLAEVQESL 430 A Y++ E L+ IG YQ+G + + D++I + +I + Q +E L+ E+L Sbjct: 381 AKYEEVELLLQIGEYQKGQDKEADQAIERMGAIRGWLCQGTHE---LSHFNETL 431 >PA1104 [NU] COG1157 Flagellar biosynthesis/type III secretory pathway ATPase Length = 451 Score = 404 bits (1037), Expect = e-112, Method: Compositional matrix adjust. Identities = 209/419 (49%), Positives = 288/419 (68%), Gaps = 6/419 (1%) Query: 19 INGKVTQVIGLMVESEGPDASIGDVCYIYPAKGNKPLQ--AEVVGFRDNKVLLMPLGELQ 76 + G++ +++GL +E+EG A++G C + G P+Q AEV+GF +KV LMP+G L Sbjct: 27 VEGRLLRMVGLTLEAEGLQAAVGSRCNVINESGYHPVQVEAEVMGFSGSKVYLMPVGSLA 86 Query: 77 AIGPGCDVVGTGKPLSVQVGSELLGKVLDGLGQPLDGSLIPARMPHSSTFNIPS-NPLNR 135 I PG VV + +G +LG+VLDG G+ LDG R + P+ NPL R Sbjct: 87 GIAPGARVVPLPDTGRLPMGMSMLGRVLDGAGRALDGK-GGMRAEDWVPMDGPTINPLKR 145 Query: 136 PRVQEPISIGVRAIDGLLTIGKGQRVGIFAGSGVGKSTLMGMIARNTSADVNVIALIGER 195 + EP+ +G+R+I+GLLT+G+GQR+G+FAG+GVGKS L+GM+ R T AD+ V+ LIGER Sbjct: 146 HPISEPLDVGIRSINGLLTVGRGQRLGLFAGTGVGKSVLLGMMTRFTRADIIVVGLIGER 205 Query: 196 GREVLDFIERDLGPEGLQRSVVVVATSDQPALIRIKGALIATTIAEYFRDRGLNVMLMMD 255 GREV +FI+ LG EGL+RSVVV + +D L+R++ A T IAEYFRD+G NV+L+MD Sbjct: 206 GREVKEFIDEILGEEGLKRSVVVASPADDAPLMRLRAAQYCTRIAEYFRDKGKNVLLLMD 265 Query: 256 SVTRYAMAQREVGLAVGEPPAMRGYTPSVFASLPKLLERAGTGPT--GSITAFYTVLVDG 313 S+TRYA AQRE+ LA+GEPPA +GY PSVFA LPKL+ERAG GSITAFYTVL +G Sbjct: 266 SLTRYAQAQREIALAIGEPPATKGYPPSVFAKLPKLVERAGNAEAGGGSITAFYTVLSEG 325 Query: 314 DDMNEPIADAVRGILDGHIVLNRNIANKGHFPAIDVLSSISRVMKDIAPEEQIAAAENVK 373 DD +PIADA RG+LDGH VL+R +A +GH+PAID+ +SISRVM + E + A+ K Sbjct: 326 DDQQDPIADAARGVLDGHFVLSRRLAEEGHYPAIDIEASISRVMPQVVEAEHLRDAQRFK 385 Query: 374 RLMAVYKDSEDLINIGAYQRGSNAQIDESIHYIDSIWNFTKQKVNEKVTLAEVQESLIS 432 +L + Y+ S DLI++GAY G + + D +I + F +Q ++E +LAE + L S Sbjct: 386 QLWSRYQQSRDLISVGAYVAGGDPETDLAIARFPVMRQFLRQGLDESESLAESRARLAS 444 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.137 0.387 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 32,495,587 Number of extensions: 1453838 Number of successful extensions: 5449 Number of sequences better than 1.0e-05: 106 Number of HSP's gapped: 5225 Number of HSP's successfully gapped: 106 Length of query: 437 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 328 Effective length of database: 25,807,025 Effective search space: 8464704200 Effective search space used: 8464704200 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 117 (49.7 bits)