BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0586.0053.p (261 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_flgE [N] COG4786 Flagellar basal body rod protein 249 3e-66 BH2449 [N] COG4786 Flagellar basal body rod protein 246 2e-65 TM1542 [N] COG4786 Flagellar basal body rod protein 152 5e-37 STM1542 [ER] COG1063 Threonine dehydrogenase and related Zn-depe... 152 5e-37 aq_834 [N] COG4786 Flagellar basal body rod protein 147 2e-35 >BS_flgE [N] COG4786 Flagellar basal body rod protein Length = 264 Score = 249 bits (636), Expect = 3e-66, Method: Compositional matrix adjust. Identities = 139/261 (53%), Positives = 176/261 (67%), Gaps = 8/261 (3%) Query: 1 MRGFQTKLDVIGNNIANVNTIGFKSGRVMFKDIMSQTVSGVTAPVDGGQGGVNAKQIGLG 60 M+ FQTKLDVIGNNIANVNT+GFK RV FKD++SQT++G +A GG N+KQIGLG Sbjct: 12 MKNFQTKLDVIGNNIANVNTVGFKKSRVTFKDMVSQTIAGGSAA-GATIGGTNSKQIGLG 70 Query: 61 VSIGSIDTMHLAGSAMTTNNPTDLRIDGDGFFLVKLSDEQATPFLTRAGDFHVDSARNLL 120 S G+IDT+H + +T DL IDGDG+F + D A TRAG+F++D+ L+ Sbjct: 71 SSSGTIDTIHSTSATQSTGRTLDLAIDGDGYFRIDTGDGTA---YTRAGNFYLDNTGTLV 127 Query: 121 TSDGLHVLNSAGEPIQLNEGTTAFSISADGSVLQTLQDGTIDSDTKIGVAKVSNPQGLEK 180 T DG HVLN G I++ FSI +DG V +G +IG+ +N GL+K Sbjct: 128 TGDGYHVLNMNGGTIKIPTDAQTFSIGSDGKVSIVDAEGKTQDGGQIGIVTFANSDGLDK 187 Query: 181 IGGNLYRMTLNANADGALNPTTANNTEEGTGAIVAGQLEMSNVDLTGEFTEMIVSQRGFQ 240 IG NLYR +LN+ A N + GTGA+ +G LEMSNVDLT EFTEMIV+QRGFQ Sbjct: 188 IGSNLYRESLNSGTASAANQPG----DGGTGALKSGFLEMSNVDLTDEFTEMIVAQRGFQ 243 Query: 241 ANSRIITTSDEVLQEVVNLKR 261 +NS+IITTSDE+LQE+VNLKR Sbjct: 244 SNSKIITTSDEILQELVNLKR 264 >BH2449 [N] COG4786 Flagellar basal body rod protein Length = 263 Score = 246 bits (629), Expect = 2e-65, Method: Compositional matrix adjust. Identities = 132/261 (50%), Positives = 175/261 (67%), Gaps = 9/261 (3%) Query: 1 MRGFQTKLDVIGNNIANVNTIGFKSGRVMFKDIMSQTVSGVTAPVDGGQGGVNAKQIGLG 60 MR FQTKLDVIGNNIANVNT G+K GR F+D++SQ ++G TAP +GGVN +Q+GLG Sbjct: 12 MRNFQTKLDVIGNNIANVNTFGYKKGRTTFQDMVSQQLAGATAP-GANRGGVNPRQVGLG 70 Query: 61 VSIGSIDTMHLAGSAMTTNNPTDLRIDGDGFFLVKLSDEQATPFLTRAGDFHVDSARNLL 120 + + SID +H GS TN DL I GDGFF V+ D T + TR+G+F+ D ++ Sbjct: 71 MQLASIDNVHTQGSLQNTNRELDLGISGDGFFQVQDGD---TFYYTRSGNFYFDQQGTIV 127 Query: 121 TSDGLHVLNSAGEPIQLNEGTTAFSISADGSVLQTLQDGTIDSDTKIGVAKVSNPQGLEK 180 + GL ++++ G PI + +FSI DG+V +DG + + + +NP+GL K Sbjct: 128 NAQGLRLMDTNGNPITVPTTAQSFSIGQDGTVTYIDEDGDLQEAGMVRLVNFANPEGLAK 187 Query: 181 IGGNLYRMTLNANADGALNPTTANNTEEGTGAIVAGQLEMSNVDLTGEFTEMIVSQRGFQ 240 G NLY ++ N+ A P + GTG I+AG LEMSNVDL+ EFTEMIV+QRGFQ Sbjct: 188 AGANLYLVSANSGA-----PLFTSAGNGGTGDIIAGTLEMSNVDLSEEFTEMIVAQRGFQ 242 Query: 241 ANSRIITTSDEVLQEVVNLKR 261 AN+RIITTSDE+LQE+VNLKR Sbjct: 243 ANTRIITTSDEILQELVNLKR 263 >TM1542 [N] COG4786 Flagellar basal body rod protein Length = 261 Score = 152 bits (384), Expect = 5e-37, Method: Compositional matrix adjust. Identities = 99/261 (37%), Positives = 138/261 (52%), Gaps = 11/261 (4%) Query: 1 MRGFQTKLDVIGNNIANVNTIGFKSGRVMFKDIMSQTVSGVTAPVDGGQGGVNAKQIGLG 60 M Q KLD I NN+ANV+T G+K R F+D++ Q V P +G G Sbjct: 12 MSAQQFKLDTIANNLANVDTTGYKKVRAEFQDLLYQYVKNAGTPTAATSSLPTGLYVGHG 71 Query: 61 VSIGSIDTMHLAGSAMTTNNPTDLRIDGDGFFLVKLSDEQATPFLTRAGDFHVDSARNLL 120 V + + G+ T N DL I GDGFF ++L D + TR G F +DS ++ Sbjct: 72 VRTAATTRIFTLGNFEQTGNALDLAIAGDGFFQIQLQDGRIA--YTRDGSFKMDSEGRIV 129 Query: 121 TSDGLHVLNSAGEPIQLNEGTTAFSISADGSVLQTLQDGTIDSDTKIGVAKVSNPQGLEK 180 TS+GL ++ I + E + ++S DG V LQDGTI I + + NP GL+ Sbjct: 130 TSNGLLLVPE----ITIPENAVSINVSPDGIVSAELQDGTIQELGTITLVRFVNPSGLKS 185 Query: 181 IGGNLYRMTLNANADGALNPTTANNTEEGTGAIVAGQLEMSNVDLTGEFTEMIVSQRGFQ 240 IG NLY T A G +P ++G GAI G LE SNVD+ E +MI +QR ++ Sbjct: 186 IGDNLYIAT---PASG--DPIEGVPGQDGFGAIKQGFLEKSNVDVVREMVDMITAQRAYE 240 Query: 241 ANSRIITTSDEVLQEVVNLKR 261 NSR+I T+DE+L+ N+KR Sbjct: 241 FNSRVIQTADEMLRTATNVKR 261 >STM1542 [ER] COG1063 Threonine dehydrogenase and related Zn-dependent dehydrogenases Length = 341 Score = 152 bits (384), Expect = 5e-37, Method: Compositional matrix adjust. Identities = 20/261 (7%), Positives = 42/261 (16%), Gaps = 11/261 (4%) Query: 1 MRGFQTKLDVIGNNIANVNTIGFKSGRVMFKDIMSQTVSGVTAPVDGGQGGVNAKQIGLG 60 +D V +G + + + V + G Sbjct: 12 GEVETRWVDYPHKKENEVLIKVDAAGICGSDIGAFRGTNPLVTYPRIIGHEVTGIVLQEG 71 Query: 61 VSIGSIDTMHLAGSAMTTNNPTDLRIDGDGFFLVKLSDEQATPFLTRAGDFHVDSARNLL 120 + G + + G Sbjct: 72 AGMPDNIKEGDRVIVDPYIYCGHCYPCSVGRTNCCENLNVIG--VHVDGAMQEVVTHPAH 129 Query: 121 TSDGLHVLNSAGEPIQLNEGTTAFSISADGSVLQTLQDGTIDSDTKIGVAKVSNPQGLEK 180 + ++ +I+ ++ G + G + Sbjct: 130 LIHKIPDNVP----SEMAPLAEPLTIALHALHRANVKAGEYVAIIGAGAIGLMAALSARH 185 Query: 181 IGGNLYRMTLNANADGALNPTTANNTEEGTGAIVAGQLEMSNVDLTGEFTEMIVSQRGFQ 240 + + A A + + T V Sbjct: 186 YKATPILID---IVEE--RLRYAQKLGVPYVLNAADDQVIDAIKNITHGTMAQVVIEASG 240 Query: 241 ANSRIITTSDEVLQEVVNLKR 261 ANS I T D Sbjct: 241 ANSAIRNTLDLASFAGRISFT 261 >aq_834 [N] COG4786 Flagellar basal body rod protein Length = 262 Score = 147 bits (371), Expect = 2e-35, Method: Compositional matrix adjust. Identities = 96/263 (36%), Positives = 141/263 (53%), Gaps = 14/263 (5%) Query: 1 MRGFQTKLDVIGNNIANVNTIGFKSGRVMFKDIMSQTVSGVTAPVDGGQGGVNAKQIGLG 60 M QT LDVI +N+ANVNT+G+K R F+D++ QTV AP + QIGLG Sbjct: 12 MTAQQTNLDVISHNMANVNTVGYKKMRATFQDLLYQTVEEPGAPTSPTTRSPSGFQIGLG 71 Query: 61 VSIGSIDTMHLAGSAMTTNNPTDLRIDGDGFFLVKLSDEQATPFLTRAGDFHVDSARNLL 120 + + G+ T+N D+ I GDGFF + L D T TR G F +DS ++ Sbjct: 72 TYVSDTYGIFTQGNLTKTDNQLDIAIQGDGFFKIVLPD--GTIAYTRNGQFRLDSEGRIV 129 Query: 121 TSDGLHVLNSAGEP-IQLNEGTTAFSISADGSVLQTLQDGT-IDSDTKIGVAKVSNPQGL 178 SDG + +P I + + SI+ADG+V Q T ++ +I +AK NP GL Sbjct: 130 NSDGYPL-----DPEITIPADAISVSIAADGTVSVLRQGATEVEEVGRIELAKFVNPAGL 184 Query: 179 EKIGGNLYRMTLNANADGALNPTTANNTEEGTGAIVAGQLEMSNVDLTGEFTEMIVSQRG 238 +IG NLY + A G P N +G G ++ G LE SNV++ E +I++QR Sbjct: 185 RRIGNNLY---IQTTASG--EPIIDNPGNQGLGTLLQGYLESSNVNIVEEMVNLIIAQRA 239 Query: 239 FQANSRIITTSDEVLQEVVNLKR 261 ++ N++ I +DE+L + NL+R Sbjct: 240 YEFNTKGIQAADEMLSQAANLRR 262 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.313 0.131 0.360 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 18,689,921 Number of extensions: 792864 Number of successful extensions: 10882 Number of sequences better than 1.0e-05: 45 Number of HSP's gapped: 10775 Number of HSP's successfully gapped: 54 Length of query: 261 Length of database: 38,378,431 Length adjustment: 104 Effective length of query: 157 Effective length of database: 26,383,695 Effective search space: 4142240115 Effective search space used: 4142240115 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 115 (48.9 bits)