BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0586.0045.p (89 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value YPO0708 [NU] COG1987 Flagellar biosynthesis pathway, component FliQ 57 9e-09 YPO1820a [NU] COG1987 Flagellar biosynthesis pathway, component ... 52 1e-07 STM1420 [U] COG4794 Type III secretory pathway, component EscS 49 1e-06 YPCD1.45 [U] COG4794 Type III secretory pathway, component EscS 47 7e-06 >YPO0708 [NU] COG1987 Flagellar biosynthesis pathway, component FliQ Length = 89 Score = 56.6 bits (135), Expect = 9e-09, Method: Compositional matrix adjust. Identities = 32/80 (40%), Positives = 44/80 (55%) Query: 1 MNTEFIIGLAGQAVYLVLETSAPMXXXXXXXXXXXSIFQATTQIQEQTLAFIPKIVAVLL 60 M T+ + ++LVL S SIFQATTQI EQTL+F+P++V +L Sbjct: 1 MTTDIAGDIIAMGLHLVLMISIVAIVPSLLIGLLVSIFQATTQINEQTLSFLPRLVMTML 60 Query: 61 ALLLFGPWIITKLVDFTGQI 80 L+ G W++ KL DFT I Sbjct: 61 VLIFAGKWMMIKLSDFTVSI 80 >YPO1820a [NU] COG1987 Flagellar biosynthesis pathway, component FliQ Length = 246 Score = 52.4 bits (124), Expect = 1e-07, Method: Compositional matrix adjust. Identities = 7/54 (12%), Positives = 13/54 (24%) Query: 36 SIFQATTQIQEQTLAFIPKIVAVLLALLLFGPWIITKLVDFTGQILGSLNMYIG 89 + + + + A LL + F II + S Sbjct: 36 GQSWSLPVQTLVFITTLSFLPAALLMMTSFTRIIIVLGLLRNAMGTPSAPPNQV 89 >STM1420 [U] COG4794 Type III secretory pathway, component EscS Length = 88 Score = 49.3 bits (116), Expect = 1e-06, Method: Compositional matrix adjust. Identities = 28/81 (34%), Positives = 47/81 (58%) Query: 1 MNTEFIIGLAGQAVYLVLETSAPMXXXXXXXXXXXSIFQATTQIQEQTLAFIPKIVAVLL 60 MN + Q +++VL TS P+ S+ QA TQIQ+QTL F+ K++A+ + Sbjct: 1 MNDSELTQFVTQLLWIVLFTSMPVVLVASVVGVIVSLVQALTQIQDQTLQFMIKLLAIAI 60 Query: 61 ALLLFGPWIITKLVDFTGQIL 81 L++ PW+ L+++T QI+ Sbjct: 61 TLMVSYPWLSGILLNYTRQIM 81 >YPCD1.45 [U] COG4794 Type III secretory pathway, component EscS Length = 88 Score = 47.0 bits (110), Expect = 7e-06, Method: Compositional matrix adjust. Identities = 29/76 (38%), Positives = 40/76 (52%) Query: 1 MNTEFIIGLAGQAVYLVLETSAPMXXXXXXXXXXXSIFQATTQIQEQTLAFIPKIVAVLL 60 M+ II QA++LVL S P S+ QA TQIQEQTL F+ K++AV++ Sbjct: 1 MSQGDIIHFTSQALWLVLVLSMPPVLVAAVVGTLVSLVQALTQIQEQTLGFVIKLIAVVV 60 Query: 61 ALLLFGPWIITKLVDF 76 L W+ +L F Sbjct: 61 TLFATASWLGNELHSF 76 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.327 0.142 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 4,861,101 Number of extensions: 103355 Number of successful extensions: 384 Number of sequences better than 1.0e-05: 7 Number of HSP's gapped: 384 Number of HSP's successfully gapped: 7 Length of query: 89 Length of database: 38,378,431 Length adjustment: 59 Effective length of query: 30 Effective length of database: 31,573,725 Effective search space: 947211750 Effective search space used: 947211750 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 109 (46.6 bits)