BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0586.0044.p (263 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value STM0910 [R] COG5525 Bacteriophage tail assembly protein 92 6e-19 YPCD1.46 [U] COG4791 Type III secretory pathway, component EscT 88 1e-17 YPO1819 [NU] COG1684 Flagellar biosynthesis pathway, component FliR 82 9e-16 ZescT [U] COG4791 Type III secretory pathway, component EscT 80 3e-15 ECs4581 [U] COG4791 Type III secretory pathway, component EscT 80 3e-15 >STM0910 [R] COG5525 Bacteriophage tail assembly protein Length = 702 Score = 92.4 bits (228), Expect = 6e-19, Method: Compositional matrix adjust. Identities = 13/254 (5%), Positives = 37/254 (14%), Gaps = 12/254 (4%) Query: 8 QNFPVFLLIFCRISAFFVVVPVFSTRSIPATFKIGLSFFVA-LVIFSSRGTGIAVPQD-- 64 V + + + + Sbjct: 12 VRREVAEYLRPPRRMPVAEGIKQFMFVPRGANTAVPWDDTLTPYMNE---AINTLSKREY 68 Query: 65 -LTYILLIAREALIGLLLGFIAYLMFMAIQTAGSFIDIQIGFGIANVIDPMTGTSAPVIG 123 AR L+ + + + + + T Sbjct: 69 DAVIFAGPARTGKTLGLIDGWIVYGIVCDPADMLVVQMTETKAREHSKTRLARTFHHS-P 127 Query: 124 NFKYLIATLLFLSMNGHHYLLDAIVYSYKWVPIDNNLLIKMIDGSLSEFLVRSFAQSFML 183 + ++ + D W I + D + Sbjct: 128 EVRKRLSPSRNDNNVHDKMFRDGSFLKIGWPSIT---VFSSSDYKRVALTDYD-RFPENI 183 Query: 184 AFQMSAPLVTALFLTDIGLAFLARTAPQYNVFVIGVPLKIIVXXXXXXXXXXXXXXXFQN 243 + + + T A + + V + + Sbjct: 184 DGEGDGFSLASKRTTTFMSAGMTLAESSPGREITDVKWRRSSPHEAPPTTGILSLYNRGD 243 Query: 244 LFDIMFESMHNLLG 257 + H Sbjct: 244 RRRWYWPCPHCGDW 257 >YPCD1.46 [U] COG4791 Type III secretory pathway, component EscT Length = 261 Score = 88.2 bits (217), Expect = 1e-17, Method: Compositional matrix adjust. Identities = 61/203 (30%), Positives = 109/203 (53%), Gaps = 4/203 (1%) Query: 22 AFFVVVPVFSTRSIPATF-KIGLSFFVALVIFSSRGTGIAVPQD-LTYILLIAREALIGL 79 A FV++PV S + + + G+ +AL ++ + + D T +LLI +E ++GL Sbjct: 22 ACFVILPVLSKQLLGGVLLRNGIVCSLALYVYPAVANQPYIEVDAFTLMLLIGKEIILGL 81 Query: 80 LLGFIAYLMFMAIQTAGSFIDIQIGFGIANVIDPMTGTSAPVIGNFKYLIATLLFLSMNG 139 L+GF+A + F A+++AG +D Q G +A++++P + G +F S Sbjct: 82 LIGFVATIPFWALESAGFIVDNQRGAAMASLLNPGLDSQTSPTGLLLTQTLITIFFSGGA 141 Query: 140 HHYLLDAIVYSYKWVPIDNNLLIKMIDGSLSEFLVRSFAQSFMLAFQMSAPLVTALFLTD 199 LL A+ +SY P+ + + +F F+Q ++A ++APL+ A+FL + Sbjct: 142 FLSLLSALFHSYVNWPVAS--FFPAVSEQWVDFFYNQFSQILLIAAVLAAPLLIAMFLAE 199 Query: 200 IGLAFLARTAPQYNVFVIGVPLK 222 GLA ++R AP NVFV+ +P+K Sbjct: 200 FGLALISRFAPSLNVFVLAMPIK 222 >YPO1819 [NU] COG1684 Flagellar biosynthesis pathway, component FliR Length = 260 Score = 82.0 bits (201), Expect = 9e-16, Method: Compositional matrix adjust. Identities = 70/245 (28%), Positives = 114/245 (46%), Gaps = 6/245 (2%) Query: 18 CRISAFFVVVPVFSTRSIPATFKIGLSFFVALVIFSSRGTGIAVPQDLTYILLIA-REAL 76 R+ A P+ S + I KIGL + +I S + +P + L +A ++ L Sbjct: 21 VRVLALIGTAPLLSEKQINKKVKIGLGVLITFLIAPSLPP-VNIPLFSSAALWVAIQQIL 79 Query: 77 IGLLLGFIAYLMFMAIQTAGSFIDIQIGFGIANVIDPMTGTSAPVIGNFKYLIATLLFLS 136 IG+ LG F A++ +G I +Q+G A DP G + PV+ ++ TLLFLS Sbjct: 80 IGVALGVTMQFAFAAVRLSGEVIGLQMGLSFATFFDPSGGPNMPVLSRLLNILVTLLFLS 139 Query: 137 MNGHHYLLDAIVYSYKWVPIDNNLLIKMIDGSLSEFLVRSFAQSFMLAFQMSAPLVTALF 196 +GH +L+ + S+ +PI ++G+ L +S + FM ++ PL+T L Sbjct: 140 FDGHLWLISLLADSFHTLPIQ----FAPLNGNGFLTLAQSGSMIFMNGLMLALPLITLLL 195 Query: 197 LTDIGLAFLARTAPQYNVFVIGVPLKIIVXXXXXXXXXXXXXXXFQNLFDIMFESMHNLL 256 ++ L L R PQ +VFVIG PL + V ++LF F+ + +L Sbjct: 196 TLNMALGMLNRMTPQLSVFVIGFPLTLTVGIISLGLIMPLLAPFTEHLFSEFFDRLAEVL 255 Query: 257 GLMGK 261 M Sbjct: 256 SGMAN 260 >ZescT [U] COG4791 Type III secretory pathway, component EscT Length = 258 Score = 80.1 bits (196), Expect = 3e-15, Method: Compositional matrix adjust. Identities = 60/216 (27%), Positives = 110/216 (50%), Gaps = 18/216 (8%) Query: 17 FC--RISAFFVVVPVFSTRSIPATF---KIGLSFFVALVI----FSSRGTGIAVPQDLTY 67 +C R F+++P+FST + + F I ++F + +++ FS + +P + Sbjct: 13 YCILRPLGMFIILPIFSTGVLLSNFIRNSIMIAFTLPIIVENYTFSEK-----LPSGIFQ 67 Query: 68 ILLIA-REALIGLLLGFIAYLMFMAIQTAGSFIDIQIGFGIANVIDPMTGTSAPVIGNFK 126 + IA +E IG +G ++F AI AG ID G I+++ +P S+ + G Sbjct: 68 LTGIALKEISIGFFIGLSFTILFWAIDAAGQIIDTLRGSTISSIFNPSISDSSSITGVIL 127 Query: 127 YLIATLLFLSMNGHHYLLDAIVYSYKWVPIDNNLLIKMIDGSLSEFLVRSFAQSFMLAFQ 186 Y +++F+ G +LD + SY+ +P+ ++ + +L +FL + L Sbjct: 128 YQFISVIFVIHGGIQSILDKLYLSYEILPLQADI---AFNRALIDFLFSLWDSFIKLMLS 184 Query: 187 MSAPLVTALFLTDIGLAFLARTAPQYNVFVIGVPLK 222 S P++ +FL D+G FL +TAPQ NVF + +P+K Sbjct: 185 FSVPMIIGIFLCDMGFGFLNKTAPQLNVFTLSLPVK 220 >ECs4581 [U] COG4791 Type III secretory pathway, component EscT Length = 258 Score = 80.1 bits (196), Expect = 3e-15, Method: Compositional matrix adjust. Identities = 60/216 (27%), Positives = 110/216 (50%), Gaps = 18/216 (8%) Query: 17 FC--RISAFFVVVPVFSTRSIPATF---KIGLSFFVALVI----FSSRGTGIAVPQDLTY 67 +C R F+++P+FST + + F I ++F + +++ FS + +P + Sbjct: 13 YCILRPLGMFIILPIFSTGVLLSNFIRNSIMIAFTLPIIVENYTFSEK-----LPSGIFQ 67 Query: 68 ILLIA-REALIGLLLGFIAYLMFMAIQTAGSFIDIQIGFGIANVIDPMTGTSAPVIGNFK 126 + IA +E IG +G ++F AI AG ID G I+++ +P S+ + G Sbjct: 68 LTGIALKEISIGFFIGLSFTILFWAIDAAGQIIDTLRGSTISSIFNPSISDSSSITGVIL 127 Query: 127 YLIATLLFLSMNGHHYLLDAIVYSYKWVPIDNNLLIKMIDGSLSEFLVRSFAQSFMLAFQ 186 Y +++F+ G +LD + SY+ +P+ ++ + +L +FL + L Sbjct: 128 YQFISVIFVIHGGIQSILDKLYLSYEILPLQADI---AFNRALIDFLFSLWDSFIKLMLS 184 Query: 187 MSAPLVTALFLTDIGLAFLARTAPQYNVFVIGVPLK 222 S P++ +FL D+G FL +TAPQ NVF + +P+K Sbjct: 185 FSVPMIIGIFLCDMGFGFLNKTAPQLNVFTLSLPVK 220 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.333 0.146 0.433 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 16,808,965 Number of extensions: 656794 Number of successful extensions: 1620 Number of sequences better than 1.0e-05: 11 Number of HSP's gapped: 1606 Number of HSP's successfully gapped: 11 Length of query: 263 Length of database: 38,378,431 Length adjustment: 104 Effective length of query: 159 Effective length of database: 26,383,695 Effective search space: 4195007505 Effective search space used: 4195007505 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (22.0 bits) S2: 115 (48.9 bits)