BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0586.0043.p (364 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value YPO1790 [NU] COG1377 Flagellar biosynthesis pathway, component FlhB 221 2e-57 STM1914 [NU] COG1377 Flagellar biosynthesis pathway, component FlhB 220 4e-57 ZflhB [NU] COG1377 Flagellar biosynthesis pathway, component FlhB 213 3e-55 ECs2590 [NU] COG1377 Flagellar biosynthesis pathway, component FlhB 213 4e-55 ZflhB [NU] COG1377 Flagellar biosynthesis pathway, component FlhB 213 5e-55 >YPO1790 [NU] COG1377 Flagellar biosynthesis pathway, component FlhB Length = 386 Score = 221 bits (562), Expect = 2e-57, Method: Compositional matrix adjust. Identities = 125/353 (35%), Positives = 191/353 (54%), Gaps = 10/353 (2%) Query: 17 DKTEKATPKKRQEARKKGQVAKXXXXXXXXXXXXXXXXXMMFGGFIKERVVKLYTDV--F 74 +K+E+ T K ++AR+KGQ+ + M G + ++ + F Sbjct: 8 EKSEEPTASKLEKAREKGQIPRSRELTSMLMLGAGLTILWMSGESMARQLSAMVAQGLHF 67 Query: 75 QNRMMMQVTPENVSEMFRHYGL---QILILLAPLFIITFVMALVVNLAEVGFMMVTEGLT 131 + M+ N +M R G+ Q L+ + P+F ++AL V + G + E + Sbjct: 68 DHSMV-----SNDKQMLRQIGMLLRQTLLAMLPIFAGLVIVALAVPMLLGGVLFSGESIK 122 Query: 132 PKFSKINPIKGFKNIFSMRSFVEFLKSIFKLLIIGYLVYSTLWGEKESFASLAHVSAEGA 191 +++P+ G K +FS ++ E LK+I K ++G++ LW L A Sbjct: 123 FDLKRMSPVAGLKRMFSSQALAELLKAILKATLVGWVTGLFLWHNWPDMMRLIAAPPVAA 182 Query: 192 YRFAAKMTMNLGIKIGAVLLIMAVFDYIYQKYEHEKSLKMSKQDIKDEYKKMEGDPIIKG 251 A + + G+ + L M FD YQ H K L+M+KQDI+DE+K EGDP +KG Sbjct: 183 LGDALHLIIFCGLVVVLGLSPMVGFDVFYQITSHIKKLRMTKQDIRDEFKNQEGDPHVKG 242 Query: 252 KIREXXXXXXXXXXXXEVPNADVIITNPTHFAVALKYDGSQMEAPQIIAKGQDFVALRIR 311 +IR+ +VP ADVI+TNPTH+AVAL+Y+ S+M AP+++AKG VALRIR Sbjct: 243 RIRQQQRAMARRRMMVDVPKADVIVTNPTHYAVALQYNESKMSAPKVLAKGAGAVALRIR 302 Query: 312 ELAKEHGVITMENKPLARALFQRTEIGDVVPADLFQAVAEVLAYVYKLNGKRR 364 EL EH + +E PLARALF+ +E+G +PA L+ AVAEVLA+VY+L +R Sbjct: 303 ELGAEHRIPLLEAPPLARALFRHSEVGQHIPATLYAAVAEVLAWVYQLKRWKR 355 >STM1914 [NU] COG1377 Flagellar biosynthesis pathway, component FlhB Length = 383 Score = 220 bits (560), Expect = 4e-57, Method: Compositional matrix adjust. Identities = 122/352 (34%), Positives = 193/352 (54%), Gaps = 10/352 (2%) Query: 17 DKTEKATPKKRQEARKKGQVAKXXXXXXXXXXXXXXXXXMMFGGFIKERVVKLYTDVFQN 76 DKTE TP + ++AR++GQ+ + G + ++ + + Sbjct: 8 DKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVCIIWFGGESLARQLAGMLSAGLHF 67 Query: 77 RMMMQVTPENVSEMFRHYGLQILILLAPLFIITFVMALVVNLAEVGFMMVTEGLTPKFSK 136 M P + ++ L PL ++AL+ + G + + L PKFSK Sbjct: 68 DHRMVNDPNLILGQIILLIKAAMMALLPLIAGVVLVALISPVMLGGLIFSGKSLQPKFSK 127 Query: 137 INPIKGFKNIFSMRSFVEFLKSIFKLLIIGYLVYSTLWGEKESFASLAHVSAEGAYRFAA 196 +NP+ G K +FS ++ E LK++ K ++G + LW + + + AE A Sbjct: 128 LNPLPGIKRMFSAQTGAELLKAVLKSTLVGCVTGFYLW---HHWPQMMRLMAESPI-VAM 183 Query: 197 KMTMNLGIKIGAVLLIMAV-----FDYIYQKYEHEKSLKMSKQDIKDEYKKMEGDPIIKG 251 ++L + + A+L+++ V FD +Q + H K L+MS+QDI+DE+K+ EGDP +KG Sbjct: 184 GNALDL-VGLCALLVVLGVIPMVGFDVFFQIFSHLKKLRMSRQDIRDEFKESEGDPHVKG 242 Query: 252 KIREXXXXXXXXXXXXEVPNADVIITNPTHFAVALKYDGSQMEAPQIIAKGQDFVALRIR 311 KIR+ +VP ADVI+TNPTH++VAL+YD ++M AP+++AKG +ALRIR Sbjct: 243 KIRQMQRAAAQRRMMEDVPKADVIVTNPTHYSVALQYDENKMSAPKVVAKGAGLIALRIR 302 Query: 312 ELAKEHGVITMENKPLARALFQRTEIGDVVPADLFQAVAEVLAYVYKLNGKR 363 E+ EH V T+E PLARAL++ EIG +P L+ AVAEVLA+V++L R Sbjct: 303 EIGAEHRVPTLEAPPLARALYRHAEIGQQIPGQLYAAVAEVLAWVWQLKRWR 354 >ZflhB [NU] COG1377 Flagellar biosynthesis pathway, component FlhB Length = 382 Score = 213 bits (543), Expect = 3e-55, Method: Compositional matrix adjust. Identities = 122/352 (34%), Positives = 194/352 (55%), Gaps = 10/352 (2%) Query: 17 DKTEKATPKKRQEARKKGQVAKXXXXXXXXXXXXXXXXXMMFGGFIKERVVKLYTDVFQN 76 DKTE TP + ++AR++GQ+ + G + R+ + + Sbjct: 7 DKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSVIWFGGVSLARRLSGMLSAGLHF 66 Query: 77 RMMMQVTPENVSEMFRHYGLQILILLAPLFIITFVMALVVNLAEVGFMMVTEGLTPKFSK 136 + P + + ++ L PL ++AL+ + G + + L PKFSK Sbjct: 67 DHSIIKDPNLILGQIILLIREAMLALLPLISGVVLVALISPVMLGGLVFSGKSLQPKFSK 126 Query: 137 INPIKGFKNIFSMRSFVEFLKSIFKLLIIGYLVYSTLWGEKESFASLAHVSAEGAYRFAA 196 +NP+ G K +FS ++ E LK+I K +++G + LW + + + AE A Sbjct: 127 LNPLPGIKRMFSAQTGAELLKAILKTILVGSVTGFFLW---HHWPHMMRLMAESPIT-AM 182 Query: 197 KMTMNLGIKIGAVLLIMAV-----FDYIYQKYEHEKSLKMSKQDIKDEYKKMEGDPIIKG 251 M+L + + A+L+++ V FD +Q + H K L+MS+QDI+DE+K+ EGDP +KG Sbjct: 183 GNAMDL-VGLCALLVVLGVIPMVGFDVFFQIFSHLKKLRMSRQDIRDEFKQSEGDPHVKG 241 Query: 252 KIREXXXXXXXXXXXXEVPNADVIITNPTHFAVALKYDGSQMEAPQIIAKGQDFVALRIR 311 +IR+ +VP ADVI+ NPTH++VAL+YD ++M AP+++AKG VALRIR Sbjct: 242 RIRQMQRAAARRRMMADVPKADVIVNNPTHYSVALQYDENKMSAPKVVAKGAGLVALRIR 301 Query: 312 ELAKEHGVITMENKPLARALFQRTEIGDVVPADLFQAVAEVLAYVYKLNGKR 363 E+ E+ V T+E PLARAL++ EIG +P L+ AVAEVLA+V++L R Sbjct: 302 EIGXENNVPTLEAPPLARALYRHVEIGQQIPGQLYAAVAEVLAWVWQLKRWR 353 >ECs2590 [NU] COG1377 Flagellar biosynthesis pathway, component FlhB Length = 382 Score = 213 bits (542), Expect = 4e-55, Method: Compositional matrix adjust. Identities = 122/352 (34%), Positives = 194/352 (55%), Gaps = 10/352 (2%) Query: 17 DKTEKATPKKRQEARKKGQVAKXXXXXXXXXXXXXXXXXMMFGGFIKERVVKLYTDVFQN 76 DKTE TP + ++AR++GQ+ + G + R+ + + Sbjct: 7 DKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSVIWFGGVSLARRLSGMLSAGLHF 66 Query: 77 RMMMQVTPENVSEMFRHYGLQILILLAPLFIITFVMALVVNLAEVGFMMVTEGLTPKFSK 136 + P + + ++ L PL ++AL+ + G + + L PKFSK Sbjct: 67 DHSIIKDPNLILGQIILLIREAMLALLPLISGVVLVALISPVMLGGLVFSGKSLQPKFSK 126 Query: 137 INPIKGFKNIFSMRSFVEFLKSIFKLLIIGYLVYSTLWGEKESFASLAHVSAEGAYRFAA 196 +NP+ G K +FS ++ E LK+I K +++G + LW + + + AE A Sbjct: 127 LNPLPGIKRMFSAQTGAELLKAILKTILVGSVTGFFLW---HHWPHMMRLMAESPIT-AM 182 Query: 197 KMTMNLGIKIGAVLLIMAV-----FDYIYQKYEHEKSLKMSKQDIKDEYKKMEGDPIIKG 251 M+L + + A+L+++ V FD +Q + H K L+MS+QDI+DE+K+ EGDP +KG Sbjct: 183 GNAMDL-VGLCALLVVLGVIPMVGFDVFFQIFSHLKKLRMSRQDIRDEFKQSEGDPHVKG 241 Query: 252 KIREXXXXXXXXXXXXEVPNADVIITNPTHFAVALKYDGSQMEAPQIIAKGQDFVALRIR 311 +IR+ +VP ADVI+ NPTH++VAL+YD ++M AP+++AKG VALRIR Sbjct: 242 RIRQMQRAAARRRMMADVPKADVIVNNPTHYSVALQYDENKMSAPKVVAKGAGLVALRIR 301 Query: 312 ELAKEHGVITMENKPLARALFQRTEIGDVVPADLFQAVAEVLAYVYKLNGKR 363 E+ E+ V T+E PLARAL++ EIG +P L+ AVAEVLA+V++L R Sbjct: 302 EIGAENNVPTLEAPPLARALYRHVEIGQQIPGQLYAAVAEVLAWVWQLKRWR 353 Score = 213 bits (541), Expect = 5e-55, Method: Compositional matrix adjust. Identities = 122/352 (34%), Positives = 194/352 (55%), Gaps = 10/352 (2%) Query: 17 DKTEKATPKKRQEARKKGQVAKXXXXXXXXXXXXXXXXXMMFGGFIKERVVKLYTDVFQN 76 DKTE TP + ++AR++GQ+ + G + R+ + + Sbjct: 7 DKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSVIWFGGVSLARRLSGMLSAGLHF 66 Query: 77 RMMMQVTPENVSEMFRHYGLQILILLAPLFIITFVMALVVNLAEVGFMMVTEGLTPKFSK 136 + P + + ++ L PL ++AL+ + G + + L PKFSK Sbjct: 67 DHSIIKDPNLILGQIILLIREAMLALLPLISGVVLVALISPVMLGGLVFSGKSLQPKFSK 126 Query: 137 INPIKGFKNIFSMRSFVEFLKSIFKLLIIGYLVYSTLWGEKESFASLAHVSAEGAYRFAA 196 +NP+ G K +FS ++ E LK+I K +++G + LW + + + AE A Sbjct: 127 LNPLPGIKRMFSAQTGAELLKAILKTILVGSVTGFFLW---HHWPHMMRLMAESPIT-AM 182 Query: 197 KMTMNLGIKIGAVLLIMAV-----FDYIYQKYEHEKSLKMSKQDIKDEYKKMEGDPIIKG 251 M+L + + A+L+++ V FD +Q + H K L+MS+QDI+DE+K+ EGDP +KG Sbjct: 183 GNAMDL-VGLCALLVVLGVIPMVGFDVFFQIFSHLKKLRMSRQDIRDEFKQSEGDPHVKG 241 Query: 252 KIREXXXXXXXXXXXXEVPNADVIITNPTHFAVALKYDGSQMEAPQIIAKGQDFVALRIR 311 +IR+ +VP ADVI+ NPTH++VAL+YD ++M AP+++AKG VALRIR Sbjct: 242 RIRQMQRAAARRRMMADVPKADVIVNNPTHYSVALQYDENKMSAPKVVAKGAGLVALRIR 301 Query: 312 ELAKEHGVITMENKPLARALFQRTEIGDVVPADLFQAVAEVLAYVYKLNGKR 363 E+ E+ V T+E PLARAL++ EIG +P L+ AVAEVLA+V++L R Sbjct: 302 EIGXENNVPTLEAPPLARALYRHVEIGQQIPGQLYAAVAEVLAWVWQLKRWR 353 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.323 0.138 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 22,243,299 Number of extensions: 813961 Number of successful extensions: 4389 Number of sequences better than 1.0e-05: 14 Number of HSP's gapped: 4371 Number of HSP's successfully gapped: 14 Length of query: 364 Length of database: 38,378,431 Length adjustment: 108 Effective length of query: 256 Effective length of database: 25,922,359 Effective search space: 6636123904 Effective search space used: 6636123904 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 116 (49.3 bits)