BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0586.0042.p (533 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ZflhA [NU] COG1298 Flagellar biosynthesis pathway, component FlhA 330 3e-90 ECs2589 [NU] COG1298 Flagellar biosynthesis pathway, component FlhA 330 3e-90 flhA [NU] COG1298 Flagellar biosynthesis pathway, component FlhA 330 5e-90 ZflhA [NU] COG1298 Flagellar biosynthesis pathway, component FlhA 330 5e-90 fhiA [NU] COG1298 Flagellar biosynthesis pathway, component FlhA 249 9e-66 >ZflhA [NU] COG1298 Flagellar biosynthesis pathway, component FlhA Length = 692 Score = 330 bits (846), Expect = 3e-90, Method: Compositional matrix adjust. Identities = 188/472 (39%), Positives = 266/472 (56%), Gaps = 9/472 (1%) Query: 25 PIPAWLLDVXXXXXXXXXXXXXXVAMNTKDPLQFSIFPSXXXXXXXXXXXXNISTTKLIL 84 P+PA++LD+ VAM T+ L+F+ FP+ N+++T++IL Sbjct: 38 PLPAFILDLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIIL 97 Query: 85 SDGH-----AGEVVATFGSWIARGQIAXXXXXXXXXXXXXXXXXTKGSERVAEVGARFTL 139 +GH AG+VV FG ++ G A TKG+ R+AEVGARF L Sbjct: 98 MEGHTGAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVL 157 Query: 140 DAMPGKQMSIDADLNAGMINEQQAGERRRKIEREADFFGSMDGASKFVKGDAIASXXXXX 199 D MPGKQM+IDADLNAG+I E +A +RR ++ +EADF+GSMDGASKFV+GDAIA Sbjct: 158 DGMPGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMV 217 Query: 200 XXXXXXXXXXMSVHGMSFQAALSTYSVLTIGDGLVSQIPALLISTAAGLIVTRAASDGNL 259 + HGMS A +Y++LTIGDGLV+QIPAL+ISTAAG+IVTR ++D ++ Sbjct: 218 INVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQDV 277 Query: 260 AEDLTGQLLSYPKLLYIVAVTIAFLGFFTPITVMSTLPXXXXXXXXXXTMSQKASRQQIA 319 E + QL S P ++ + A + LG + M L R+Q A Sbjct: 278 GEQMVNQLFSNPSVMLLSAAVLGLLGL---VPGMPNLVFLLFTAGLLGLAWWIRGREQKA 334 Query: 320 XXXXXXXXXXXXXRSPESVINLLSV-DAIEFEFGYGLIPLADTGQGGDLLDRIIMIRRQC 378 E+ N + + D++ E GY LIP+ D Q G+LL RI IR++ Sbjct: 335 PAEPKPVKMAENNAVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRKKF 394 Query: 379 ALEMGLVVPVIRIRDNIQLKPNEYVIKIKXXXXXXXXXXXXHYLAMSPGYEDESIHGIET 438 A EMG + PV+ IRDN+ L+P Y I +K +LA++PG ++ G T Sbjct: 395 AQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLPGEAT 454 Query: 439 VEPSFGLPALWIDESVKERAELSGYTVVDPPSVVATHLTELIKRYGHELLGR 490 V+P+FGL A+WI+ ++KE+A++ GYTVV+ +VVATHL LI ++ EL GR Sbjct: 455 VDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGR 506 >ECs2589 [NU] COG1298 Flagellar biosynthesis pathway, component FlhA Length = 692 Score = 330 bits (846), Expect = 3e-90, Method: Compositional matrix adjust. Identities = 188/472 (39%), Positives = 266/472 (56%), Gaps = 9/472 (1%) Query: 25 PIPAWLLDVXXXXXXXXXXXXXXVAMNTKDPLQFSIFPSXXXXXXXXXXXXNISTTKLIL 84 P+PA++LD+ VAM T+ L+F+ FP+ N+++T++IL Sbjct: 38 PLPAFILDLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIIL 97 Query: 85 SDGH-----AGEVVATFGSWIARGQIAXXXXXXXXXXXXXXXXXTKGSERVAEVGARFTL 139 +GH AG+VV FG ++ G A TKG+ R+AEVGARF L Sbjct: 98 MEGHTGAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVL 157 Query: 140 DAMPGKQMSIDADLNAGMINEQQAGERRRKIEREADFFGSMDGASKFVKGDAIASXXXXX 199 D MPGKQM+IDADLNAG+I E +A +RR ++ +EADF+GSMDGASKFV+GDAIA Sbjct: 158 DGMPGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMV 217 Query: 200 XXXXXXXXXXMSVHGMSFQAALSTYSVLTIGDGLVSQIPALLISTAAGLIVTRAASDGNL 259 + HGMS A +Y++LTIGDGLV+QIPAL+ISTAAG+IVTR ++D ++ Sbjct: 218 INVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQDV 277 Query: 260 AEDLTGQLLSYPKLLYIVAVTIAFLGFFTPITVMSTLPXXXXXXXXXXTMSQKASRQQIA 319 E + QL S P ++ + A + LG + M L R+Q A Sbjct: 278 GEQMVNQLFSNPSVMLLSAAVLGLLGL---VPGMPNLVFLLFTAGLLGLAWWIRGREQKA 334 Query: 320 XXXXXXXXXXXXXRSPESVINLLSV-DAIEFEFGYGLIPLADTGQGGDLLDRIIMIRRQC 378 E+ N + + D++ E GY LIP+ D Q G+LL RI IR++ Sbjct: 335 PAEPKPVKMAENNAVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRKKF 394 Query: 379 ALEMGLVVPVIRIRDNIQLKPNEYVIKIKXXXXXXXXXXXXHYLAMSPGYEDESIHGIET 438 A EMG + PV+ IRDN+ L+P Y I +K +LA++PG ++ G T Sbjct: 395 AQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLPGEAT 454 Query: 439 VEPSFGLPALWIDESVKERAELSGYTVVDPPSVVATHLTELIKRYGHELLGR 490 V+P+FGL A+WI+ ++KE+A++ GYTVV+ +VVATHL LI ++ EL GR Sbjct: 455 VDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGR 506 >flhA [NU] COG1298 Flagellar biosynthesis pathway, component FlhA Length = 692 Score = 330 bits (845), Expect = 5e-90, Method: Compositional matrix adjust. Identities = 188/472 (39%), Positives = 266/472 (56%), Gaps = 9/472 (1%) Query: 25 PIPAWLLDVXXXXXXXXXXXXXXVAMNTKDPLQFSIFPSXXXXXXXXXXXXNISTTKLIL 84 P+PA++LD+ VAM T+ L+F+ FP+ N+++T++IL Sbjct: 38 PLPAFILDLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIIL 97 Query: 85 SDGH-----AGEVVATFGSWIARGQIAXXXXXXXXXXXXXXXXXTKGSERVAEVGARFTL 139 +GH AG+VV FG ++ G A TKG+ R+AEVGARF L Sbjct: 98 MEGHTGAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVL 157 Query: 140 DAMPGKQMSIDADLNAGMINEQQAGERRRKIEREADFFGSMDGASKFVKGDAIASXXXXX 199 D MPGKQM+IDADLNAG+I E +A +RR ++ +EADF+GSMDGASKFV+GDAIA Sbjct: 158 DGMPGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMV 217 Query: 200 XXXXXXXXXXMSVHGMSFQAALSTYSVLTIGDGLVSQIPALLISTAAGLIVTRAASDGNL 259 + HGMS A +Y++LTIGDGLV+QIPAL+ISTAAG+IVTR ++D ++ Sbjct: 218 INIVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQDV 277 Query: 260 AEDLTGQLLSYPKLLYIVAVTIAFLGFFTPITVMSTLPXXXXXXXXXXTMSQKASRQQIA 319 E + QL S P ++ + A + LG + M L R+Q A Sbjct: 278 GEQMVNQLFSNPSVMLLSAAVLGLLGL---VPGMPNLVFLLFTAGLLGLAWWIRGREQKA 334 Query: 320 XXXXXXXXXXXXXRSPESVINLLSV-DAIEFEFGYGLIPLADTGQGGDLLDRIIMIRRQC 378 E+ N + + D++ E GY LIP+ D Q G+LL RI IR++ Sbjct: 335 PAEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRKKF 394 Query: 379 ALEMGLVVPVIRIRDNIQLKPNEYVIKIKXXXXXXXXXXXXHYLAMSPGYEDESIHGIET 438 A EMG + PV+ IRDN+ L+P Y I +K +LA++PG ++ G T Sbjct: 395 AQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLPGEAT 454 Query: 439 VEPSFGLPALWIDESVKERAELSGYTVVDPPSVVATHLTELIKRYGHELLGR 490 V+P+FGL A+WI+ ++KE+A++ GYTVV+ +VVATHL LI ++ EL GR Sbjct: 455 VDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGR 506 Score = 330 bits (845), Expect = 5e-90, Method: Compositional matrix adjust. Identities = 188/472 (39%), Positives = 266/472 (56%), Gaps = 9/472 (1%) Query: 25 PIPAWLLDVXXXXXXXXXXXXXXVAMNTKDPLQFSIFPSXXXXXXXXXXXXNISTTKLIL 84 P+PA++LD+ VAM T+ L+F+ FP+ N+++T++IL Sbjct: 38 PLPAFILDLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIIL 97 Query: 85 SDGH-----AGEVVATFGSWIARGQIAXXXXXXXXXXXXXXXXXTKGSERVAEVGARFTL 139 +GH AG+VV FG ++ G A TKG+ R+AEVGARF L Sbjct: 98 MEGHTGAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVL 157 Query: 140 DAMPGKQMSIDADLNAGMINEQQAGERRRKIEREADFFGSMDGASKFVKGDAIASXXXXX 199 D MPGKQM+IDADLNAG+I E +A +RR ++ +EADF+GSMDGASKFV+GDAIA Sbjct: 158 DGMPGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMV 217 Query: 200 XXXXXXXXXXMSVHGMSFQAALSTYSVLTIGDGLVSQIPALLISTAAGLIVTRAASDGNL 259 + HGMS A +Y++LTIGDGLV+QIPAL+ISTAAG+IVTR ++D ++ Sbjct: 218 INVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQDV 277 Query: 260 AEDLTGQLLSYPKLLYIVAVTIAFLGFFTPITVMSTLPXXXXXXXXXXTMSQKASRQQIA 319 E + QL S P ++ + A + LG + M L R+Q A Sbjct: 278 GEQMVNQLFSNPSVMLLSAAVLGLLGL---VPGMPNLVFLLFTAGLLGLAWWIRGREQKA 334 Query: 320 XXXXXXXXXXXXXRSPESVINLLSV-DAIEFEFGYGLIPLADTGQGGDLLDRIIMIRRQC 378 E+ N + + D++ E GY LIP+ D Q G+LL RI IR++ Sbjct: 335 PAEPKPVKMAENNAVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRKKF 394 Query: 379 ALEMGLVVPVIRIRDNIQLKPNEYVIKIKXXXXXXXXXXXXHYLAMSPGYEDESIHGIET 438 A EMG + PV+ IRDN+ L+P Y I +K +LA++PG ++ G T Sbjct: 395 AQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLPGEAT 454 Query: 439 VEPSFGLPALWIDESVKERAELSGYTVVDPPSVVATHLTELIKRYGHELLGR 490 V+P+FGL A+WI+ ++KE+A++ GYTVV+ +VVATHL LI ++ EL GR Sbjct: 455 VDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGR 506 >fhiA [NU] COG1298 Flagellar biosynthesis pathway, component FlhA Length = 579 Score = 249 bits (636), Expect = 9e-66, Method: Compositional matrix adjust. Identities = 151/409 (36%), Positives = 218/409 (53%), Gaps = 17/409 (4%) Query: 124 TKGSERVAEVGARFTLDAMPGKQMSIDADLNAGMINEQQAGERRRKIEREADFFGSMDGA 183 TKG+ER++EV ARFTLDAMPGKQM+IDADLNAG+IN+ QA RR+ + EADF+G+MDGA Sbjct: 19 TKGAERISEVSARFTLDAMPGKQMAIDADLNAGLINQAQAQTRRKDVASEADFYGAMDGA 78 Query: 184 SKFVKGDAIASXXXXXXXXXXXXXXXMSVHGMSFQAALSTYSVLTIGDGLVSQIPALLIS 243 SKFV+GDAIA + + +S AA Y ++TIGDGLV+QIP+LL+S Sbjct: 79 SKFVRGDAIAGMMILAINLIGGVCIGIFKYNLSADAAFQQYVLMTIGDGLVAQIPSLLLS 138 Query: 244 TAAGLIVTRAASDGNLAEDLTGQLLSYPKLLYIVAVTIAFLGFFTPITVMSTLPXXXXXX 303 TAA +IVTR + +G++A D+ QLL+ P +LY A I F+ P M LP Sbjct: 139 TAAAIIVTRVSDNGDIAHDVRNQLLASPSVLY-TATGIMFVLAVVP--GMPHLPFLLFSA 195 Query: 304 XXXXTMSQKASRQQIAXXXXXXXXXXXXXRSPESVINLLS------VDAIEFEFGYGLIP 357 T + S+Q +A E+ +S ++ I GY L+ Sbjct: 196 LLGFT-GWRMSKQPLAAEAEEKSLETLTRTITETSEQQVSWETIPLIEPISLSLGYKLVA 254 Query: 358 LADTGQGGDLLDRIIMIRRQCALEMGLVVPVIRIRDNIQLKPNEYVIKIKXXXXXXXXXX 417 L D QG L RI +R+ + G+++P IRIR+N +LKP++Y I I Sbjct: 255 LVDKAQGNPLTQRIRGVRQVISDGNGVLLPEIRIRENFRLKPSQYAIFINGIKADEADIP 314 Query: 418 XXHYLAMSPGYEDESIHGIETVEPSFGLPALWIDESVKERAELSGYTVVDPPSVVATHLT 477 +A+ I G++ +P++G+P WI + K +A GY V+D SV+ATH+ Sbjct: 315 ADKLMALPSSETYGEIDGVQGNDPAYGMPVTWIQAAQKAKALNMGYQVIDSASVIATHVN 374 Query: 478 ELIKRYGHELLGRXXXXGNQATCRQSPRKLSCAGGRACAFRTGHRGYSK 526 ++++ Y +L N Q +LS R + YS+ Sbjct: 375 KIVRSYIPDLF-------NYDDITQLHNRLSSTAPRLAEDLSAALNYSQ 416 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.135 0.387 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 29,012,275 Number of extensions: 949504 Number of successful extensions: 1387 Number of sequences better than 1.0e-05: 17 Number of HSP's gapped: 1344 Number of HSP's successfully gapped: 17 Length of query: 533 Length of database: 38,378,431 Length adjustment: 111 Effective length of query: 422 Effective length of database: 25,576,357 Effective search space: 10793222654 Effective search space used: 10793222654 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 118 (50.1 bits)