BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0586.0019.p (348 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH2420 [M] COG0750 Predicted membrane-associated Zn-dependent pr... 187 3e-47 BS_yluC [M] COG0750 Predicted membrane-associated Zn-dependent p... 163 5e-40 CAC1796 [M] COG0750 Predicted membrane-associated Zn-dependent p... 97 5e-20 slr1821 [M] COG0750 Predicted membrane-associated Zn-dependent p... 96 6e-20 all3971 [M] COG0750 Predicted membrane-associated Zn-dependent p... 93 6e-19 >BH2420 [M] COG0750 Predicted membrane-associated Zn-dependent proteases 1 Length = 420 Score = 187 bits (474), Expect = 3e-47, Method: Compositional matrix adjust. Identities = 115/307 (37%), Positives = 172/307 (56%), Gaps = 20/307 (6%) Query: 20 IAVRLGQDNKVKNIYLDSLDTRRNVIRGEAQYTDLENELKIR-LDVDGEVTVYDVHPQAM 78 + + + N V I +++ + + DLE EL I D DGE +DV P+A Sbjct: 83 VGLVFNEKNVVSRIIVNNKSKHPEAQVVQVERIDLERELFIEAYDDDGERKRWDVDPKAE 142 Query: 79 LIKGNQQTQIAPKDRQFGSKTVGQRAIAIFAGPVMNXXXXXXXXXXHLQMAGIKIENPTY 138 +++ + TQIAP DRQFGSK+V QRA+AIFAGP+MN + M GI +E+P Sbjct: 143 MVQDEEATQIAPYDRQFGSKSVAQRALAIFAGPLMNFVLAFVLLAAYGFMQGIPVEDPV- 201 Query: 139 VKIGDVSANMPAEEAGLQKGDIVVSINGEKIGGDYQKMISLTSASKGKEMQWTLQRGEET 198 +G+++ N AE AGLQKGD V+SI+G+ + + M + +E+ + ++R + Sbjct: 202 --VGNIAENSAAETAGLQKGDYVLSIDGQTL-ETWVDMTMIIQQHPNEEITFEVERAGQI 258 Query: 199 YNVSMIPRSMEGEEGGKVGIT------PELPTRKAG--FGETITKSGTAMV-DTTKLIFI 249 + + P +EG +G +G+ PE T +G FG T T + M+ D +L+ Sbjct: 259 LQIPVTPNQVEGMDGEPIGLVGIERPAPEPATLVSGLQFGATQTYTYMTMIFDVLRLLVT 318 Query: 250 GFKQLINKFNMNDISGPVGTFQMTGQIAQQGIQYLTYWAAILSLYLGIFNLLPIPALDGS 309 G +F+++ ++GPVG TGQ A+ GI L W A LS+ LGI NLLP+PALDG Sbjct: 319 G------QFSLDYVAGPVGIVNYTGQAAEMGIFVLLQWTAALSVNLGIVNLLPLPALDGG 372 Query: 310 RLVFLGV 316 RLVFLG+ Sbjct: 373 RLVFLGL 379 >BS_yluC [M] COG0750 Predicted membrane-associated Zn-dependent proteases 1 Length = 422 Score = 163 bits (412), Expect = 5e-40, Method: Compositional matrix adjust. Identities = 101/302 (33%), Positives = 165/302 (54%), Gaps = 8/302 (2%) Query: 19 TIAVRLGQDNKVKNIYLDSLDTRRNVIRGEAQYTDLENELKIRLDVDG---EVTVYDVHP 75 T+ + ++++V+ + ++ + + + E + DLE+++KI G E++ + V Sbjct: 84 TVGLLFNKEDQVEKVIINQKEKYPDALVIEVETADLEHDMKITGYEQGKEDELSSFTVSE 143 Query: 76 QAMLIKGNQQTQIAPKDRQFGSKTVGQRAIAIFAGPVMNXXXXXXXXXXHLQMAGIKIEN 135 + I ++ QIAP +RQFGSK V QR AI AGP+MN + G+ Sbjct: 144 TSFFIVDGEEVQIAPYNRQFGSKPVWQRIKAIAAGPIMNFILAYVILVMLGLIQGVPSNE 203 Query: 136 PTYVKIGDVSANMPAEEAGLQKGDIVVSINGEKIGGDYQKMISLTSASKGKEMQWTLQRG 195 P +G ++ N A EAGL++GD + SINGEK+ + ++S + KEM ++R Sbjct: 204 PM---LGQLTDNGRAAEAGLKEGDYIQSINGEKMR-SWTDIVSAVKENPEKEMDVAVKRD 259 Query: 196 EETYNVSMIPRSMEGEEGGKVGITPELPTRKAGFGETITKSGTAMVDTTKLIFIGFKQLI 255 +T ++S+ P +++ E +G + G + T+ VD TK I +L+ Sbjct: 260 NKTLHISVTPEAVKDENKKTIGRFGSYAPTEKGVLSAVAYGATSTVDVTKAILTNLSKLV 319 Query: 256 N-KFNMNDISGPVGTFQMTGQIAQQGIQYLTYWAAILSLYLGIFNLLPIPALDGSRLVFL 314 +F ++ +SGPVG + MT Q+A+ GI L +AA LS+ LGI NLLPIPALDG RL+FL Sbjct: 320 TGQFKLDMLSGPVGIYDMTDQVAKTGIVNLFQFAAFLSINLGIVNLLPIPALDGGRLLFL 379 Query: 315 GV 316 + Sbjct: 380 FI 381 >CAC1796 [M] COG0750 Predicted membrane-associated Zn-dependent proteases 1 Length = 339 Score = 96.7 bits (239), Expect = 5e-20, Method: Compositional matrix adjust. Identities = 71/230 (30%), Positives = 116/230 (50%), Gaps = 23/230 (10%) Query: 93 RQFGSKTVGQRAIAIFAGPVMNXXXXXXXXXXHLQMAGIKIENPTYVKIGDVSANMPAEE 152 R F +K+ +R + AGP+MN GI + PT +G +SAN PA++ Sbjct: 80 RAFNNKSSARRLSIVIAGPIMNLILAAVLFCIVGMSEGIAL--PT---VGKISANSPAQK 134 Query: 153 AGLQKGDIVVSINGEKIGGDYQKMISLTSASKGKEMQWTLQRGEETYNVSMIPRSMEGEE 212 G++ GD +V IN + ++ + + +KG+ ++ L+ V+++P+ + E+ Sbjct: 135 IGIKAGDTIVKINNYSVH-TWEDISFNMALNKGEGIKLALKNNGTIKKVTLVPQYSKKEK 193 Query: 213 GGKVGITPEL---PT----RKAGFGETITKSGTAMVDTTKLIFIGFKQLIN-KFNMNDIS 264 +GI+P+ PT K G ET+T K +++ K ++ K + D+S Sbjct: 194 MYLIGISPKFIDKPTIIEGAKYGTSETVT--------MIKTVYLSLKMMVTGKASAKDVS 245 Query: 265 GPVGTFQMTGQIAQQGIQYLTYWAAILSLYLGIFNLLPIPALDGSRLVFL 314 GPV ++TG A G L + A +S LG+ NLLPIPALDG VFL Sbjct: 246 GPVSIIKVTGAAANAGFIRLVNFIAFISAQLGVMNLLPIPALDGG-FVFL 294 >slr1821 [M] COG0750 Predicted membrane-associated Zn-dependent proteases 1 Length = 366 Score = 96.3 bits (238), Expect = 6e-20, Method: Compositional matrix adjust. Identities = 83/276 (30%), Positives = 130/276 (47%), Gaps = 23/276 (8%) Query: 50 QYTDLENELKIR-LDVDGEVTVYDVHPQAMLIKGNQQTQIAPKD--RQFGSKTVGQRAIA 106 +Y E E IR + + G V D P + + P D ++ + RAI Sbjct: 48 KYQGAETEYSIRAIPLGGYVAFPDDDPDSEI----------PADDPNLLKNRPILDRAIV 97 Query: 107 IFAGPVMNXXXXXXXXXXHLQMAGIKIENPTYVKIGDVSANMPAEEAGLQKGDIVVSING 166 I AG + N + G + P V I V + A+ AG++ GDIV+S+ G Sbjct: 98 ISAGVIANLVFAYFLLIGQVSTIGFQNIQPGLV-IPQVDSASAAQVAGMEPGDIVLSLQG 156 Query: 167 EKIGG---DYQKMISLTSASKGKEMQWTLQRGEETYNVSMIPRSMEGEEGGKVGIT--PE 221 + G + I + S + +QRGEET +++ P + + E GK+G+ P Sbjct: 157 NTLPGFPDATTQFIDIVRRSPSVPITVEVQRGEETKTLTITP-TPDAEGKGKIGVALLPN 215 Query: 222 LPTRKAGFG-ETITKSGTAMVDTTKLIFIGFKQLINKF--NMNDISGPVGTFQMTGQIAQ 278 + T++A E +T S A KL GF QLI+ F N + ++GPV + IA+ Sbjct: 216 VETKRASNPLEALTYSAEAFERIVKLTTQGFWQLISNFADNASQVAGPVKIVEYGANIAR 275 Query: 279 QGIQYLTYWAAILSLYLGIFNLLPIPALDGSRLVFL 314 L + A++S+ L + N+LP+PALDG +LVFL Sbjct: 276 SDASNLFQFGALISINLAVINILPLPALDGGQLVFL 311 >all3971 [M] COG0750 Predicted membrane-associated Zn-dependent proteases 1 Length = 364 Score = 93.2 bits (230), Expect = 6e-19, Method: Compositional matrix adjust. Identities = 63/221 (28%), Positives = 109/221 (49%), Gaps = 9/221 (4%) Query: 103 RAIAIFAGPVMNXXXXXXXXXXHLQMAGIKIENPTYVKIGDVSANMPA--EEAGLQKGDI 160 RAI I AG + N + GI + V I ++ + A AGL+ GD+ Sbjct: 91 RAIVISAGVIANLIFAYMLLLAQVGFVGIGQASQPGVSIQQLAPEVSAVATNAGLKPGDV 150 Query: 161 VVSINGEKIGGDYQKMISL---TSASKGKEMQWTLQRGEETYNVSMIPRSMEGEEGGKVG 217 ++S N ++ G Q++ +L S GK +Q T+ RG+E +V++IP + +G Sbjct: 151 ILSANQKEFGTSLQEIEALRDIIKNSPGKSIQLTVARGDERLSVNVIPEAKPAGGSIGIG 210 Query: 218 ITP--ELPTRKAGFGETITKSGTAMVDTTKLIFIGFKQLINKF--NMNDISGPVGTFQMT 273 + P ++ R + + + + F GF QL+ F + ++GP+ ++ Sbjct: 211 LAPNGKVERRPVSLSKAFSVGASEFQRIVVMTFKGFGQLVTNFGETASQVAGPIKIVEIG 270 Query: 274 GQIAQQGIQYLTYWAAILSLYLGIFNLLPIPALDGSRLVFL 314 IAQ L ++AA++S+ L + N+LP+PALDG +L FL Sbjct: 271 ANIAQNDTGSLFFFAALISINLAVINILPLPALDGGQLAFL 311 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.137 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 23,156,391 Number of extensions: 915565 Number of successful extensions: 1527 Number of sequences better than 1.0e-05: 7 Number of HSP's gapped: 1517 Number of HSP's successfully gapped: 7 Length of query: 348 Length of database: 38,378,431 Length adjustment: 107 Effective length of query: 241 Effective length of database: 26,037,693 Effective search space: 6275084013 Effective search space used: 6275084013 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 116 (49.3 bits)