BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0586.0010.p (861 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value AGc1311 [M] COG0463 Glycosyltransferases involved in cell wall b... 590 e-168 AGc1314 [C] COG0277 FAD/FMN-containing dehydrogenases 590 e-168 SPBC1271.15c [J] COG0532 Translation initiation factor 2 (IF-2; ... 347 3e-95 YOL023w [J] COG0532 Translation initiation factor 2 (IF-2; GTPase) 322 1e-87 PH1095_3 [J] COG0532 Translation initiation factor 2 (IF-2; GTPase) 129 2e-29 >AGc1311 [M] COG0463 Glycosyltransferases involved in cell wall biogenesis Length = 913 Score = 590 bits (1520), Expect = e-168, Method: Compositional matrix adjust. Identities = 301/598 (50%), Positives = 413/598 (69%), Gaps = 10/598 (1%) Query: 268 QPVERREKIDNTPKKIIVRGSMTVGETAKLLHKDASEVIKKLISMGVMATINQELDIDTI 327 Q E REK+ +++I+ ++T+ E ++ + + A +VIK L+ G M +D D Sbjct: 320 QMQETREKV---LREVILPETITIQELSQRMSERAVDVIKFLMKEGQMLKPGDVIDADLA 376 Query: 328 LLLSSEFGVEVEVKIPVDEDSFE--TVEENDSEEDLQTRPPVVTIMGHVDHGKTTLLDAI 385 L++ EFG V+ V E E + D E ++ +RPPVVTIMGHVDHGKT+LLDAI Sbjct: 377 ELIAVEFGHTVK---RVSESDVEEGIFNQTDDEGEMVSRPPVVTIMGHVDHGKTSLLDAI 433 Query: 386 RSTNVTGGEAGGITQHIGAYQVEINHKKITFLDTPGHEAFTAMRARGAQVTDMTIIVVAA 445 R NV GEAGGITQHIGAYQVE N KITF+DTPGH AFTAMRARGAQ TD+ ++VVAA Sbjct: 434 RQANVVSGEAGGITQHIGAYQVEKNGHKITFIDTPGHAAFTAMRARGAQATDIAVLVVAA 493 Query: 446 DDGVMPQTVEAINHAKAAGLPIIVAVNKIDKPGADPDRVKQELTNYELVPEEWGGDTIFV 505 DD VMPQT+E+INHAKAAG+PI+VA+NKIDK A+PD+V+Q+L +E+ E GG+ + V Sbjct: 494 DDSVMPQTIESINHAKAAGVPIVVAINKIDKHEANPDKVRQQLLQHEVFVESMGGEVLDV 553 Query: 506 NLSAKQRINXXXXXXXXXXVAEVNEYKANPDKRARGTVIEAELDKNRGPVARILVQNGTL 565 +SAK ++N AE+ + KA+P + A GTVIEAELD+ RG VA +LVQ GTL Sbjct: 554 EVSAKNKLNLDKLLEAILLQAEILDLKADPSRTAEGTVIEAELDRGRGAVATVLVQKGTL 613 Query: 566 KVGDAFVAGNCFGRVRAMVNDKGRKIKEAGPSTPVEITGLTEVPQAGDPFMAFEDERKAR 625 K G VAG+ +GRVRA+VNDKG +KEAGP+ PVEI GL+ P AGD F E+E +AR Sbjct: 614 KPGQIIVAGDQWGRVRALVNDKGDHVKEAGPAMPVEILGLSGTPSAGDRFAVVENESRAR 673 Query: 626 AIADRRSTSQRQSEL--NTNTRVTLDDLFKHIKEGEIKDLNVIIKGDVQGSVEALKGSLA 683 I++ R R + T R +L+ + ++ +K+ ++IK DVQGSVEA+ SL Sbjct: 674 EISEYRQRLARDKAVARQTGQRGSLEQMMSQLQTSGLKEFPLVIKADVQGSVEAIIASLD 733 Query: 684 KIEVEGVRVKIIHSGAGAITESDITLAAASNAIVIGFNVRPDAQTKAAAEQEKVDVRLHN 743 K+ + VR +++HSGAGAITESDI+LA ASNA +IGFNVR +AQ + A+E+ +++R +N Sbjct: 734 KLGTDEVRARVVHSGAGAITESDISLAEASNAAIIGFNVRANAQARTASERAGIEIRYYN 793 Query: 744 IIYNVIEEIESAMKGMLDPVYKENIIGHAEVRNVFKISKVGTIAGCMVTSGKITRNAEMR 803 IIY++++++++AM G+L P +E +G+AE+ VF I+KVG +AGC V GK+ R A +R Sbjct: 794 IIYDLVDDVKAAMSGLLSPERRETFLGNAEILEVFNITKVGKVAGCRVVEGKVERGAGVR 853 Query: 804 LIRSGIVVFQGKIDTLKRFKDDAKEVAQGYECGITLERYNDVVEGDIIEAFIMETVER 861 L+R +V+ +GK+ TLKRFKD+ EV G ECG+ E Y D+ GD IE F +E + R Sbjct: 854 LVRDNVVIHEGKLKTLKRFKDEVNEVPVGQECGMAFENYEDIRAGDTIECFRVEHITR 911 >AGc1314 [C] COG0277 FAD/FMN-containing dehydrogenases Length = 913 Score = 590 bits (1520), Expect = e-168, Method: Compositional matrix adjust. Identities = 301/598 (50%), Positives = 413/598 (69%), Gaps = 10/598 (1%) Query: 268 QPVERREKIDNTPKKIIVRGSMTVGETAKLLHKDASEVIKKLISMGVMATINQELDIDTI 327 Q E REK+ +++I+ ++T+ E ++ + + A +VIK L+ G M +D D Sbjct: 320 QMQETREKV---LREVILPETITIQELSQRMSERAVDVIKFLMKEGQMLKPGDVIDADLA 376 Query: 328 LLLSSEFGVEVEVKIPVDEDSFE--TVEENDSEEDLQTRPPVVTIMGHVDHGKTTLLDAI 385 L++ EFG V+ V E E + D E ++ +RPPVVTIMGHVDHGKT+LLDAI Sbjct: 377 ELIAVEFGHTVK---RVSESDVEEGIFNQTDDEGEMVSRPPVVTIMGHVDHGKTSLLDAI 433 Query: 386 RSTNVTGGEAGGITQHIGAYQVEINHKKITFLDTPGHEAFTAMRARGAQVTDMTIIVVAA 445 R NV GEAGGITQHIGAYQVE N KITF+DTPGH AFTAMRARGAQ TD+ ++VVAA Sbjct: 434 RQANVVSGEAGGITQHIGAYQVEKNGHKITFIDTPGHAAFTAMRARGAQATDIAVLVVAA 493 Query: 446 DDGVMPQTVEAINHAKAAGLPIIVAVNKIDKPGADPDRVKQELTNYELVPEEWGGDTIFV 505 DD VMPQT+E+INHAKAAG+PI+VA+NKIDK A+PD+V+Q+L +E+ E GG+ + V Sbjct: 494 DDSVMPQTIESINHAKAAGVPIVVAINKIDKHEANPDKVRQQLLQHEVFVESMGGEVLDV 553 Query: 506 NLSAKQRINXXXXXXXXXXVAEVNEYKANPDKRARGTVIEAELDKNRGPVARILVQNGTL 565 +SAK ++N AE+ + KA+P + A GTVIEAELD+ RG VA +LVQ GTL Sbjct: 554 EVSAKNKLNLDKLLEAILLQAEILDLKADPSRTAEGTVIEAELDRGRGAVATVLVQKGTL 613 Query: 566 KVGDAFVAGNCFGRVRAMVNDKGRKIKEAGPSTPVEITGLTEVPQAGDPFMAFEDERKAR 625 K G VAG+ +GRVRA+VNDKG +KEAGP+ PVEI GL+ P AGD F E+E +AR Sbjct: 614 KPGQIIVAGDQWGRVRALVNDKGDHVKEAGPAMPVEILGLSGTPSAGDRFAVVENESRAR 673 Query: 626 AIADRRSTSQRQSEL--NTNTRVTLDDLFKHIKEGEIKDLNVIIKGDVQGSVEALKGSLA 683 I++ R R + T R +L+ + ++ +K+ ++IK DVQGSVEA+ SL Sbjct: 674 EISEYRQRLARDKAVARQTGQRGSLEQMMSQLQTSGLKEFPLVIKADVQGSVEAIIASLD 733 Query: 684 KIEVEGVRVKIIHSGAGAITESDITLAAASNAIVIGFNVRPDAQTKAAAEQEKVDVRLHN 743 K+ + VR +++HSGAGAITESDI+LA ASNA +IGFNVR +AQ + A+E+ +++R +N Sbjct: 734 KLGTDEVRARVVHSGAGAITESDISLAEASNAAIIGFNVRANAQARTASERAGIEIRYYN 793 Query: 744 IIYNVIEEIESAMKGMLDPVYKENIIGHAEVRNVFKISKVGTIAGCMVTSGKITRNAEMR 803 IIY++++++++AM G+L P +E +G+AE+ VF I+KVG +AGC V GK+ R A +R Sbjct: 794 IIYDLVDDVKAAMSGLLSPERRETFLGNAEILEVFNITKVGKVAGCRVVEGKVERGAGVR 853 Query: 804 LIRSGIVVFQGKIDTLKRFKDDAKEVAQGYECGITLERYNDVVEGDIIEAFIMETVER 861 L+R +V+ +GK+ TLKRFKD+ EV G ECG+ E Y D+ GD IE F +E + R Sbjct: 854 LVRDNVVIHEGKLKTLKRFKDEVNEVPVGQECGMAFENYEDIRAGDTIECFRVEHITR 911 >SPBC1271.15c [J] COG0532 Translation initiation factor 2 (IF-2; GTPase) Length = 686 Score = 347 bits (891), Expect = 3e-95, Method: Compositional matrix adjust. Identities = 201/587 (34%), Positives = 326/587 (55%), Gaps = 20/587 (3%) Query: 289 MTVGETAKLLHKDASEVIKKLISMGVMATINQEL-DIDTILLLSSEFGVEVEVKIPVDED 347 ++V A LL +++ L +G+ + L + LL+ E+G +V+ Sbjct: 96 LSVASFANLLQIPTRRILRDLNRLGIKDVFPEYLITYEYSSLLAEEYGFDVQEAAAYSAP 155 Query: 348 SFETVEENDSEEDLQTRPPVVTIMGHVDHGKTTLLDAIRSTNVTGGEAGGITQHIGAYQV 407 S +++ N ++ RPPVVT+MGHVDHGKTTLLDA R + + E GGITQ IGA+ V Sbjct: 156 SPKSL--NSKKDSGPLRPPVVTLMGHVDHGKTTLLDAFRKSTIASTEHGGITQKIGAFTV 213 Query: 408 EINH--KKITFLDTPGHEAFTAMRARGAQVTDMTIIVVAADDGVMPQTVEAINHAKAAGL 465 + K ITFLDTPGH AF AMR RGA + D+ ++VVA DDGV PQTVEAI H ++A + Sbjct: 214 PFDKGSKFITFLDTPGHMAFEAMRKRGANIADIVVLVVAGDDGVKPQTVEAIKHIQSADV 273 Query: 466 PIIVAVNKIDKPGADPDRVKQELTNYELVPEEWGGDTIFVNLSAKQRINXXXXXXXXXXV 525 P++VA+ K D+PG ++ ++L N + E GG+T + +SAK + Sbjct: 274 PVVVALTKSDRPGTPIHKIYEQLLNNGIQVEALGGETQIIPISAKTGKGIPELEAAILTL 333 Query: 526 AEVNEYKANPDKRARGTVIEAELDKNRGPVARILVQNGTLKVGDAFVAGNCFGRVRAMVN 585 AE+ E +A+P +G ++E+ + K G A ++V+ GT+K G VAG + +VR++V+ Sbjct: 334 AEIMEIRASPRDPFQGWIVESSVTKGVGSSATVVVKRGTVKKGMYLVAGKSWCKVRSLVD 393 Query: 586 DKGRKIKEAGPSTPVEITGLTEVPQAGDPFMAFEDERKARAIAD---RRSTSQRQSELNT 642 + IK+ P ++ G ++P AGD + E +A+ I R+S Q EL Sbjct: 394 VNKKSIKQVLPGQAAQVYGWKDLPIAGDLAYEVKSESEAKRILSDIYRQSNEQNFYELAE 453 Query: 643 NTRVTLDDLFKHIKEGEI---------KDLNVIIKGDVQGSVEALKGSLAKIEVEGVRVK 693 + K G K N+I K + GS+EAL L ++ V+ + Sbjct: 454 SQNEQRVSALAAKKSGPAAIQEETSVSKSFNIIAKCEDTGSMEALSDYLKPLQFGKVKSR 513 Query: 694 IIHSGAGAITESDITLAAASNAIVIGFNVRPDAQTKAAAEQEKVDVRLHNIIYNVIEEIE 753 ++++G G +TE+DI A S+AI+I F V T AE+ V + HN+IY++++++ Sbjct: 514 VLYTGVGPVTETDIERAETSDAIIISFGVSVPKATFRLAEKHNVKLLFHNVIYHLMDDVR 573 Query: 754 SAMKGMLDPVYKENIIGHAEVRNVFKIS---KVGTIAGCMVTSGKITRNAEMRLIRSGIV 810 L P+ + + G A + +F I V +AGC VT+G I ++ ++RL+R+ + Sbjct: 574 KLFALRLPPILVQRVTGEAIISAIFDIKAKRAVVHVAGCRVTNGTIEKSHKIRLVRNDKI 633 Query: 811 VFQGKIDTLKRFKDDAKEVAQGYECGITLERYNDVVEGDIIEAFIME 857 ++ G+ID+LK K++ + +G ECGI L+ ++++V GD ++ F+ E Sbjct: 634 IWSGEIDSLKHLKEEVTSIKKGRECGILLKNFDEIVTGDKLQTFVEE 680 >YOL023w [J] COG0532 Translation initiation factor 2 (IF-2; GTPase) Length = 676 Score = 322 bits (826), Expect = 1e-87, Method: Compositional matrix adjust. Identities = 212/608 (34%), Positives = 335/608 (55%), Gaps = 59/608 (9%) Query: 280 PKKIIVRGSMTVGETAKLLHKDASEVIKKLISMGVMATINQELDIDTILLLSSEFGVEV- 338 P + ++V + A LL+ +IK L ++G +I T +LS E+ VE+ Sbjct: 57 PLNFSIPNYISVNKLANLLNCRVERLIKDLTALGFE-------NITTTYILSKEY-VELI 108 Query: 339 --EVKIPVDEDSFETVEENDSEED--------LQTRPPVVTIMGHVDHGKTTLLDAIRST 388 E + S T +N +E L R PVVTIMGHVDHGKTT++D +R + Sbjct: 109 LQEYNFALPNLSTSTNLDNVYDELKSPVNPKLLTKRAPVVTIMGHVDHGKTTIIDYLRKS 168 Query: 389 NVTGGEAGGITQHIGAYQVEI--NHKKITFLDTPGHEAFTAMRARGAQVTDMTIIVVAAD 446 +V E GGITQHIGA+Q+ + KKITFLDTPGH AF MR RGA +TD+ ++VV+ + Sbjct: 169 SVVAQEHGGITQHIGAFQITAPKSGKKITFLDTPGHAAFLKMRERGANITDIIVLVVSVE 228 Query: 447 DGVMPQTVEAINHAKAAGLPIIVAVNKIDKPGADPDRVK--QELTNYELVP----EEWGG 500 D +MPQT+EAI HAK +G +I+A+ KID+ +R K +++ N +V E+ GG Sbjct: 229 DSLMPQTLEAIKHAKNSGNEMIIAITKIDRIPQPKEREKKIEKVINDLIVQGIPVEKIGG 288 Query: 501 DTIFVNLSAKQRINXXXXXXXXXXVAEVNEYKA--NPDKRARGTVIEAELDKNRGPVARI 558 D + +SAK N ++EV + +A +P A G +IE+++ K G VA + Sbjct: 289 DVQVIPISAKTGENMDLLEESIVLLSEVMDIRAENSPKTIAEGWIIESQVKKQVGNVATV 348 Query: 559 LVQNGTLKVGDAFVAGNCFGRVRAMVNDKGRKIKEAGPSTPVEITGLTEVPQAGDPFMAF 618 LV+ GTL+ G + GN F +++ +++DKG I +A PS E+ G +VP GD + Sbjct: 349 LVKKGTLQKGKILICGNTFCKIKNLIDDKGIPILKATPSYATEVLGWKDVPHVGDEVIQV 408 Query: 619 EDERKARAIADRRSTSQRQSELNTNTRVT--------------LDDLFKH---IKEGEI- 660 + E A+ +R Q E+ N+ + L+ +H ++E E Sbjct: 409 KSEAIAKKFISKR---QDLIEVQKNSSIVEKLNEERALAKEQHLNKELEHENTVQEHEQN 465 Query: 661 ---KDLNVIIKGDVQGSVEALKGSLAKIEVEGVRVKIIHSGAGAITESDITLAAASNAIV 717 K +N IIK DV GS EA+ S++ + + VR +I S G TESD+ +A + + + Sbjct: 466 TGPKLINYIIKCDVSGSAEAVSESISSLGNDEVRCNVISSSVGIPTESDLKMAQITESTI 525 Query: 718 IGFNVRPDAQTKAAAEQEKVDVRLHNIIYNVIEEIESAMKGMLDPVYKENIIGHAEVRNV 777 + FN+ + ++ + + ++ +N+IY +IE++ + L P++++ I+ +VR Sbjct: 526 LCFNL-GNLPSEVINNRAGIKIKQYNVIYKLIEDVTETLTENLKPIFEKKIVSTVDVRET 584 Query: 778 FKI---SKVGTIAGCMVTSGKITRNAEMRLIR--SGIVVFQGKIDTLKRFKDDAKEVAQG 832 F K+ IAGC V +G I +N+ ++++R + V+F GKI TLK KDD EV++G Sbjct: 585 FDFRLKKKIIRIAGCKVNNGVIKKNSLVQVVRGPNEDVIFDGKISTLKHNKDDVAEVSKG 644 Query: 833 YECGITLE 840 +ECGIT E Sbjct: 645 HECGITFE 652 >PH1095_3 [J] COG0532 Translation initiation factor 2 (IF-2; GTPase) Length = 578 Score = 129 bits (325), Expect = 2e-29, Method: Compositional matrix adjust. Identities = 130/457 (28%), Positives = 204/457 (44%), Gaps = 106/457 (23%) Query: 379 TTLLDAIRSTNVTGGEAGGITQHIGA------------------YQVEINHKKITFLDTP 420 TTLLD IR TNV EAGGITQHIGA ++ EI + F+DTP Sbjct: 1 TTLLDKIRKTNVAAKEAGGITQHIGATEVPIDVVKKIAGPLIKLWKAEIRLPGLLFIDTP 60 Query: 421 GHEAFTAMRARGAQVTDMTIIVVAADDGVMPQTVEAINHAKAAGLPIIVAVNKIDKPGA- 479 GHEAFT++RARG + D+ ++V+ ++G PQT+E+I + P +VA NKID+ Sbjct: 61 GHEAFTSLRARGGSLADLAVLVIDVNEGFQPQTIESIEILRRYRTPFVVAANKIDRIRGW 120 Query: 480 -----DP---------DRVKQELTN--YELVPE--EWGG-------------DTIFVNLS 508 +P R QEL +EL+ + E+G + V +S Sbjct: 121 VIEEDEPFLMNIKRQDQRAIQELETKLWELIGKFYEFGFQANRFDRVQNFTRELAIVPIS 180 Query: 509 AKQRINXXXXXXXXXXVAEV---NEYKANPDKRARGTVIEAELDKNRGPVARILVQNGTL 565 AK I +++ + K + ARGT++E + G +++ +GTL Sbjct: 181 AKYGIGIAELLVLIAGLSQKYLEEKLKIEVEGPARGTILEVREEPGLGHTIDVIIYDGTL 240 Query: 566 KVGDAFVAGN----CFGRVRAMVNDKGR-----------KIKEAGPSTPVEIT--GLTEV 608 D V G +VRA++ K + E + V+I GL E Sbjct: 241 HKDDTIVVGGKDKAIVTKVRALLKPKPLDEIRDPRFRFDYVDEVTAAAGVKIAAPGLEEA 300 Query: 609 PQAGDPFMAF---EDERKARAIADRRSTSQRQSELNTNTRVTLDDLFKHIKEGEIKDLNV 665 AG P +A ED +A + RQ E + ++ D + V Sbjct: 301 -LAGSPVIAAPTPEDVERA------KEEIMRQIE---SVVISTD------------KVGV 338 Query: 666 IIKGDVQGSVEALKGSLAKIEVEGVRVKIIHSGAGAITESDITLAAA------SNAIVIG 719 I+K D GS+EAL L + E + I + G I+++D+ A + +++G Sbjct: 339 IVKADTLGSLEALSKELQEKE-----IPIRKADVGNISKTDVMEALSVKEENPKYGVILG 393 Query: 720 FNVRPDAQTKAAAEQEKVDVRLHNIIYNVIEEIESAM 756 FNV+ + K A+ ++V + + NIIY +IE+ E+ + Sbjct: 394 FNVKVNEDAKEVAKAKEVPIFVGNIIYKLIEDYEAWI 430 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.312 0.130 0.358 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 56,482,455 Number of extensions: 2322097 Number of successful extensions: 23802 Number of sequences better than 1.0e-05: 83 Number of HSP's gapped: 23710 Number of HSP's successfully gapped: 87 Length of query: 861 Length of database: 38,378,431 Length adjustment: 115 Effective length of query: 746 Effective length of database: 25,115,021 Effective search space: 18735805666 Effective search space used: 18735805666 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.8 bits) S2: 120 (50.8 bits)