BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0584.0053.p (299 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value L172964 [IQR] COG1028 Dehydrogenases with different specificitie... 150 2e-36 L142816 [IQR] COG1028 Dehydrogenases with different specificitie... 147 2e-35 SMa0326 [IQR] COG1028 Dehydrogenases with different specificitie... 141 1e-33 VNG0364G [IQR] COG1028 Dehydrogenases with different specificiti... 132 6e-31 SMa0320 [R] COG4221 Short-chain alcohol dehydrogenase of unknown... 124 2e-28 >L172964 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 281 Score = 150 bits (379), Expect = 2e-36, Method: Compositional matrix adjust. Identities = 94/256 (36%), Positives = 134/256 (52%), Gaps = 8/256 (3%) Query: 21 DTIALITGTSSGFGLLTALALARQGCRVAATMRDISRQGELLRQAGEAGLAERIHVISLD 80 D + L+TG+S+GFG L Q +V AT R +S EL + E I ISLD Sbjct: 4 DEVWLVTGSSTGFGRALVEELIAQDYKVVATARQLSTLSELPDK-------ENILKISLD 56 Query: 81 VTDSASIQSAVAAVLDKFGRVDVLVNNXXXXXXXXXXXXXXXXWRRQMDTNFFGLIEVTK 140 VT SIQ AVA + KFGR+DVLVNN R+ MDTNF+G ++T Sbjct: 57 VTKGDSIQKAVAETIAKFGRIDVLVNNAGFGYFGVMEESDQSAVRKIMDTNFWGANDMTL 116 Query: 141 AVLPVMRRQRAGLIVNISSVSGLTGFPGYAPYAASKYAVEGFSESLRQEVLPFGIRVVLV 200 AVLP MR+Q++G I+N S GLT + Y A+K+A+EG ++LR+EV P GI V + Sbjct: 117 AVLPYMRKQKSGHILNTVSRGGLTTTENLSYYHATKFAMEGLFQTLRKEVEPLGIFVTNI 176 Query: 201 EPASFRTPIWDKGMEGMHTSVNSPYQEQLAQVLRYSRMSAETAPDPEQVAGLIARITAMK 260 EP SFRT + E S Y++ + + S +P A +++ ++ Sbjct: 177 EPGSFRTDWAGRSKESAPDKF-SDYEKAHKLLEHLNSYSGTQVGNPASAAKAFIKVSKLE 235 Query: 261 APRLRYPVGKGSRLLT 276 P + Y +GK + L+ Sbjct: 236 NPPMHYLMGKDAYQLS 251 >L142816 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 270 Score = 147 bits (371), Expect = 2e-35, Method: Compositional matrix adjust. Identities = 98/274 (35%), Positives = 146/274 (53%), Gaps = 15/274 (5%) Query: 23 IALITGTSSGFGLLTALALARQGCRVAATMRDISRQGELLRQAGEAGLAERIHVISLDVT 82 I LITG SSG G TA LA+ G +V R + + E L+ G + I LD+T Sbjct: 3 IILITGASSGIGYQTAELLAQNGHKVYGAARRMDKL-EKLKSVG-------VEPIYLDIT 54 Query: 83 DSASIQSAVAAVLDKFGRVDVLVNNXXXXXXXXXXXXXXXXWRRQMDTNFFGLIEVTKAV 142 + S + V ++D G++D+L+NN +RQ + N FGL +TK V Sbjct: 55 NEESCRKLVDTIIDNEGQIDILINNAGYGSYGAIEDVPLEEAKRQFEVNLFGLAALTKLV 114 Query: 143 LPVMRRQRAGLIVNISSVSG-LTGFPGYAPYAASKYAVEGFSESLRQEVLPFGIRVVLVE 201 LP MR Q+AG I+NISS+ G T + G A Y A+KYA+E FS++LR EV PFGI V L+E Sbjct: 115 LPHMRHQKAGRIINISSMGGRFTTYFG-AWYHATKYALEAFSDALRMEVKPFGIDVSLIE 173 Query: 202 PASFRTPIWDKGMEGMHTSVN-SPYQE---QLAQVLRYSRMSAETAPDPEQVAGLIARIT 257 P +T + + S Y+E + A +R + S DP+ ++ I++ Sbjct: 174 PGGIKTNWGLIAADHLAESAKGGTYEEAALKTATSMR-KQYSGNMMSDPKIISKTISKAV 232 Query: 258 AMKAPRLRYPVGKGSRLLTIGKTLLPWKVFEGII 291 + K R RY +G G++ L T+LP ++F+ +I Sbjct: 233 SSKRMRARYLIGFGAKPLVFLHTILPTRIFDWLI 266 >SMa0326 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 279 Score = 141 bits (356), Expect = 1e-33, Method: Compositional matrix adjust. Identities = 89/247 (36%), Positives = 121/247 (48%), Gaps = 10/247 (4%) Query: 25 LITGTSSGFGLLTALALARQGCRVAATMRDISRQGELLRQAGEAGLAERIHVISLDVTDS 84 L+TG S+GFG A L G +V T R + +L Q G+A ++ LDV D Sbjct: 10 LVTGCSTGFGRYIAEHLLEVGEKVVVTARKADKIADL-EQKGDA------LILPLDVIDR 62 Query: 85 ASIQSAVAAVLDKFGRVDVLVNNXXXXXXXXXXXXXXXXWRRQMDTNFFGLIEVTKAVLP 144 Q V A FGR+DVL+NN RR D NFFG +VLP Sbjct: 63 DQCQKVVDAAEAHFGRIDVLINNAGIGFFGAIEETDESNARRLFDVNFFGTANTIHSVLP 122 Query: 145 VMRRQRAGLIVNISSVSGLTGFPGYAPYAASKYAVEGFSESLRQEVLPFGIRVVLVEPAS 204 MR +R+G IVN++S+ GL G+ G Y A+K+AVEG S++LR EV P GI V+ VEP++ Sbjct: 123 HMRARRSGTIVNLTSIGGLVGYTGVGYYCATKFAVEGLSDTLRNEVAPLGINVMTVEPSA 182 Query: 205 FRTPIWDKGMEGMHTSVNSPYQEQLAQVLR-YSRMSAETAPDPEQVAGLIARITAMKAPR 263 FRT W + S+ Y+ + R Y + A DP + A I + P Sbjct: 183 FRTE-WAGSSNEVSASIED-YEATAGEARRAYHTSVGKQAGDPARAAKAIREAVLAQQPP 240 Query: 264 LRYPVGK 270 P+G Sbjct: 241 HHLPLGN 247 >VNG0364G [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 273 Score = 132 bits (332), Expect = 6e-31, Method: Compositional matrix adjust. Identities = 92/271 (33%), Positives = 131/271 (48%), Gaps = 13/271 (4%) Query: 21 DTIALITGTSSGFGLLTALALARQGCRVAATMRDISRQGELLRQAGEAGLAERIHVISLD 80 + +AL+TG SSG G T +L + RV AT RD + +L+ A E G LD Sbjct: 2 EKVALVTGCSSGVGAATVRSLTDEEWRVVATARDTA---DLVALAEETG----CETAELD 54 Query: 81 VTDSASIQSAVAAVLDKFGRVDVLVNNXXXXXXXXXXXXXXXXWRRQMDTNFFGLIEVTK 140 VT A ++ V +D GR+D LVNN RQ D N +G + + Sbjct: 55 VTKPAQCRAVVDDAVDDHGRLDCLVNNAGFAQLGPLEDVPPRKLHRQFDVNVYGPHRLIR 114 Query: 141 AVLPVMRRQRAGLIVNISSVSGLTGFPGYAPYAASKYAVEGFSESLRQEVLPFGIRVVLV 200 A LP MR + G IVN+SSVSG PG Y SK+A+EG S++LR EV GI V +V Sbjct: 115 AALPHMRERGEGTIVNVSSVSGRVATPGMGAYNGSKFALEGLSDALRGEVAEHGIDVAVV 174 Query: 201 EPASFRTPIWDKG---MEGMHTSVNSPYQEQLAQVLRYSRMSAETAPDPEQVAGLIARIT 257 EP T D+ ++ + S Y+ + S ++ A PE+VA +IA Sbjct: 175 EPGPVDTGFQDRADAELDALDRS--GGYERIYSWFEDASAVNGVGAVSPERVADVIATAA 232 Query: 258 AMKAPRLRYPVGKGSRLLTIGKTLLPWKVFE 288 P RYPV + R+ + + LP ++ + Sbjct: 233 VSPNPAARYPVAQAGRVGVLAR-FLPDRLLD 262 >SMa0320 [R] COG4221 Short-chain alcohol dehydrogenase of unknown specificity Length = 249 Score = 124 bits (311), Expect = 2e-28, Method: Compositional matrix adjust. Identities = 77/201 (38%), Positives = 112/201 (55%), Gaps = 7/201 (3%) Query: 12 MNSKNGQNADTIALITGTSSGFGLLTALALARQGCRVAATMRDISRQGELLRQAGEAGLA 71 M S NG+ IAL+TG SSG G TA LA G +V R + E L++ EA Sbjct: 1 MTSLNGK----IALVTGASSGIGAATAAKLAEAGAKVGIAARRTDKL-EDLKKKIEAKGG 55 Query: 72 ERIHVISLDVTDSASIQSAVAAVLDKFGRVDVLVNNXXXXXXXXXXXXXXXXWRRQMDTN 131 E + VI +DV D+ S+++ V ++D +G +D+LVNN W+R +D N Sbjct: 56 EAL-VIEMDVVDTTSVEAGVKKLVDAYGSIDILVNNAGLMPLSDIDQFKVDEWQRMVDVN 114 Query: 132 FFGLIEVTKAVLPVMRRQRAGLIVNISSVSGLTGFPGYAPYAASKYAVEGFSESLRQEV- 190 GL+ T AVLP M +Q +G + N+SS++G F G + Y A+K+AV FS+ LR EV Sbjct: 115 VKGLLNTTAAVLPQMIKQHSGHVFNMSSIAGRKVFKGLSVYCATKHAVTAFSDGLRMEVG 174 Query: 191 LPFGIRVVLVEPASFRTPIWD 211 GIRV ++P + T ++D Sbjct: 175 QKHGIRVTCIQPGAVATELYD 195 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.133 0.376 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 19,755,408 Number of extensions: 778046 Number of successful extensions: 2614 Number of sequences better than 1.0e-05: 309 Number of HSP's gapped: 2151 Number of HSP's successfully gapped: 309 Length of query: 299 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 193 Effective length of database: 26,153,027 Effective search space: 5047534211 Effective search space used: 5047534211 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 115 (48.9 bits)