BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0584.0034.p (502 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH1861 [G] COG3534 Alpha-L-arabinofuranosidase 684 0.0 BS_abfA [G] COG3534 Alpha-L-arabinofuranosidase 645 0.0 BH1874 [G] COG3534 Alpha-L-arabinofuranosidase 146 8e-35 BS_xsa [G] COG3534 Alpha-L-arabinofuranosidase 130 5e-30 >BH1861 [G] COG3534 Alpha-L-arabinofuranosidase Length = 500 Score = 684 bits (1766), Expect = 0.0, Method: Compositional matrix adjust. Identities = 322/502 (64%), Positives = 380/502 (75%), Gaps = 3/502 (0%) Query: 1 MTIKASMIVDKDFKLAEVDPRLYGSFIEHLGRAVYGGIYEPGHATADENGFRQDVLNAVR 60 MT+ A+M+VDK FK+ E+D R+YGSFIEHLGRAVY GIYEPGH DE GFR+DV+ V+ Sbjct: 1 MTLTATMVVDKSFKIGEIDKRIYGSFIEHLGRAVYEGIYEPGHPDGDEQGFRKDVIRLVQ 60 Query: 61 ALNVPIIRYPGGNFVSGYNWEDGVGPKAERKQTLELAWWTTETNHVGTNEFAEWAKLAGS 120 L VP++RYPGGNFVSGYNWEDGVGP +ER + L+LAW TTETN +GTNEF +WAK G+ Sbjct: 61 ELQVPLVRYPGGNFVSGYNWEDGVGPVSERPKRLDLAWRTTETNEIGTNEFVDWAKKVGA 120 Query: 121 EVMMAVNLGTRGIDAARNLVEYCNHPSGSYWSDLRISHGVKAPHQFKTWCLGNEMDGPWQ 180 EV MAVNLG+RG+DAARNLVEYCNHPSGSYWSDLRISHG K PH KTWCLGNEMDGPWQ Sbjct: 121 EVNMAVNLGSRGVDAARNLVEYCNHPSGSYWSDLRISHGYKDPHNIKTWCLGNEMDGPWQ 180 Query: 181 IGAKTAVEYGRLANETAKAMRWVDPSLELVACGSSGSGMSTFAEWEATVLDLTYDNVDFL 240 IG KTA EYGR+A E K M+ VDPS+ELVACGSS S M+TFA+WEATVLD TYD VD++ Sbjct: 181 IGQKTAEEYGRVAAEAGKVMKLVDPSIELVACGSSNSKMATFADWEATVLDHTYDYVDYI 240 Query: 241 SLHTYYNNNDSDTANFLAKSLDLDQFIDSVAAICDXXXXXXXXXXXXXXXXDEWNVW-KS 299 SLHTYY N D D AN+LA+S+D+D+FI SV AI D DEWNVW S Sbjct: 241 SLHTYYGNRDDDLANYLAQSMDMDEFIRSVIAIADYVKAKKRSKKTIHLSFDEWNVWFHS 300 Query: 300 QGSSRMETHWQIAPPEFEDVYNMEDALAVGCCLISLLKHADRVKMACLAQLINTIAPITT 359 + R T W +APP ED+Y EDAL VG LI+LLKHADRVK+ACLAQL+N IAPI T Sbjct: 301 NEADRQITPWSVAPPLLEDIYTFEDALLVGSMLITLLKHADRVKIACLAQLVNVIAPIMT 360 Query: 360 ENGGPLWLQTTYYPYMHASLYGRGTVLHPLITSPKYDSSDFTDVPYLEAVSVYNEELSEI 419 E GGP W QT +YPYMHAS+YGRG L I+SPKYDS DFTDVPYL+A V+ EE E+ Sbjct: 361 EKGGPAWKQTIFYPYMHASVYGRGVALQAQISSPKYDSKDFTDVPYLDAAVVHLEEAEEV 420 Query: 420 TVFAVNRHLSEGMELSVDLRSFGPTEVIQHTVMEHEDLQAANTKLHPGNVLPHDRGTAVA 479 T+FAVN+H +E + L D+RSF V++H V+EHE+++A N V PH G + Sbjct: 421 TIFAVNKHQTESLNLQCDMRSFEGYHVLEHIVLEHENMKATNQGRE--QVTPHHNGDSAI 478 Query: 480 EDGRITALLSKASWNVIRLRVK 501 + GR+TA L+K SWNVIRL K Sbjct: 479 DQGRLTANLAKLSWNVIRLGKK 500 >BS_abfA [G] COG3534 Alpha-L-arabinofuranosidase Length = 500 Score = 645 bits (1665), Expect = 0.0, Method: Compositional matrix adjust. Identities = 307/500 (61%), Positives = 372/500 (74%), Gaps = 3/500 (0%) Query: 4 KASMIVDKDFKLAEVDPRLYGSFIEHLGRAVYGGIYEPGHATADENGFRQDVLNAVRALN 63 KA MIVDK++K+ EVD R+YGSFIEH+GRAVY GIYEP H ADE+GFR+DV + ++ L Sbjct: 3 KARMIVDKEYKIGEVDKRIYGSFIEHMGRAVYEGIYEPDHPEADEDGFRKDVQSLIKELQ 62 Query: 64 VPIIRYPGGNFVSGYNWEDGVGPKAERKQTLELAWWTTETNHVGTNEFAEWAKLAGSEVM 123 VPIIRYPGGNF+SGYNWEDGVGP R + L+LAW TTETN VGTNEF W K +EV Sbjct: 63 VPIIRYPGGNFLSGYNWEDGVGPVENRPRRLDLAWQTTETNEVGTNEFLSWPKKVNTEVN 122 Query: 124 MAVNLGTRGIDAARNLVEYCNHPSGSYWSDLRISHGVKAPHQFKTWCLGNEMDGPWQIGA 183 MAVNLGTRGIDAARNLVEYCNHP GSYWSDLR SHG + P+ KTWCLGNEMDGPWQIG Sbjct: 123 MAVNLGTRGIDAARNLVEYCNHPKGSYWSDLRRSHGYEQPYGIKTWCLGNEMDGPWQIGH 182 Query: 184 KTAVEYGRLANETAKAMRWVDPSLELVACGSSGSGMSTFAEWEATVLDLTYDNVDFLSLH 243 KTA EYGRLA ETAK M+WVDPS+ELVACGSS SGM TF +WEA VL+ TY++VD++SLH Sbjct: 183 KTADEYGRLAAETAKVMKWVDPSIELVACGSSNSGMPTFIDWEAKVLEHTYEHVDYISLH 242 Query: 244 TYYNNNDSDTANFLAKSLDLDQFIDSVAAICDXXXXXXXXXXXXXXXXDEWNVW-KSQGS 302 TYY N D++ N+LA+S+DLD FI SVAA CD DEWNVW S + Sbjct: 243 TYYGNRDNNLPNYLARSMDLDHFIKSVAATCDYVKAKTRSKKTINLSLDEWNVWYHSNEA 302 Query: 303 SRMETHWQIAPPEFEDVYNMEDALAVGCCLISLLKHADRVKMACLAQLINTIAPITTENG 362 + W A P ED+YN EDAL VG LI++L+HADRVK+ACLAQL+N IAPI TE G Sbjct: 303 DKKVEPWITARPILEDIYNFEDALLVGSLLITMLQHADRVKIACLAQLVNVIAPIMTEKG 362 Query: 363 GPLWLQTTYYPYMHASLYGRGTVLHPLITSPKYDSSDFTDVPYLEAVSVYNEELSEITVF 422 G W Q +YPYMHAS+YGRG L PLI+SPKYD SDFTDVPY++A VY+EE +T+F Sbjct: 363 GEAWRQPIFYPYMHASVYGRGESLKPLISSPKYDCSDFTDVPYVDAAVVYSEEEETLTIF 422 Query: 423 AVNRHLSEGMELSVDLRSFGPTEVIQHTVMEHEDLQAANTKLHPGNVLPHDRGTAVAEDG 482 AVN+ + ME + LR F ++ +H V+EH+D++A N + + NV+PH G++ + Sbjct: 423 AVNK-AEDQMETEISLRGFESYQIAEHIVLEHQDIKATN-QHNRKNVVPHSNGSSSVSEN 480 Query: 483 RITALLSKASWNVIRLRVKS 502 +TA + SWNVIRL+ +S Sbjct: 481 GLTAHFTPLSWNVIRLKKQS 500 >BH1874 [G] COG3534 Alpha-L-arabinofuranosidase Length = 498 Score = 146 bits (368), Expect = 8e-35, Method: Compositional matrix adjust. Identities = 124/487 (25%), Positives = 201/487 (41%), Gaps = 36/487 (7%) Query: 3 IKASMIVDKDFKLAEVDPRLYGSFIEHLGRAVYGGIY-EPGHATADENGFRQDVLNAVRA 61 +K S+ V D + ++ +YG F EHLGR +Y G++ + G R DVL A++ Sbjct: 1 MKQSIKVYTDLEHGKISKHIYGHFSEHLGRCIYEGLWVGEDSPIPNTEGIRNDVLGALQE 60 Query: 62 LNVPIIRYPGGNFVSGYNWEDGVGPKAERKQTLELAW-WTTETNHVGTNEFAEWAKLAGS 120 LN+P++R+PGG F Y+W+DGVGP+ RK+ + W E NH GT+EF +L G+ Sbjct: 61 LNIPVLRWPGGCFADEYHWKDGVGPRENRKRMVNTHWGGVVENNHFGTHEFMRLCELLGA 120 Query: 121 EVMMAVNLGTRGIDAARNLVEYCNHPSGSYWSDLRISHGVKAPHQFKTWCLGNEMDGPWQ 180 E + N+G+ + + VEY S ++ R +G + P K + +GNE W Sbjct: 121 EPYICGNVGSGTVQEMQEWVEYMTFDGESPMANWRTENGREEPWALKYFGVGNE---NWG 177 Query: 181 IGAKTAVE-YGRLANETAKAMR-WVDPSLELVACGSSGSGMSTFAEWEATVLDLTYDNVD 238 G E Y L +R + D + +A G++ W ++ +D Sbjct: 178 CGGHMRPEYYADLYRRYQTYVRNYGDNKIYKIAGGANVDDY----RWTEVLMREAAHLMD 233 Query: 239 FLSLHTY-------------YNNNDSDTANFLAKSLDLDQFIDSVAAICDXXXXXXXXXX 285 LSLH Y ++ +S+ L KS +D+ + + I D Sbjct: 234 GLSLHYYTVPGDFWENKGSALDDRESEWFKTLKKSFRMDELLTKHSTIMD----RYDPEK 289 Query: 286 XXXXXXDEWNVWKSQGSSRMETHWQIAPPEFEDVYNMEDALAVGCCLISLLKHADRVKMA 345 DEW W + P + DAL G +H DRV MA Sbjct: 290 RVGLIVDEWGTW-------FDVEPGTNPGFLYQQNTIRDALVAGVHFHIFHEHNDRVHMA 342 Query: 346 CLAQLINTIAPITTENGGPLWLQTTYYPYMHASLYGRGTVLHPLITSPKYDSSDFTDVPY 405 +AQ++N + + G + L TY+ + ++ T L Y D Sbjct: 343 NIAQMVNVLQAMVLTEGEQMLLTPTYHVFNMYKVHQDATRLEVNADVGTYKFGDDELPAV 402 Query: 406 LEAVSVYNEELSEITVFAVNRHLSEGMELSVD-LRSFGPTEVIQHTVMEHEDLQAANTKL 464 + S NE ++V ++ H + L ++ L + ++ +L A NT Sbjct: 403 TVSASKDNEGAIHVSVCHLDPHRDTDLTLKLNGLNKSLSASSVSGELLTANELNAHNTFE 462 Query: 465 HPGNVLP 471 P V P Sbjct: 463 RPNEVEP 469 >BS_xsa [G] COG3534 Alpha-L-arabinofuranosidase Length = 148 Score = 130 bits (327), Expect = 5e-30, Method: Compositional matrix adjust. Identities = 0/511 (0%), Positives = 0/511 (0%), Gaps = 38/511 (7%) Query: 8 IVDKDFKLAEVDPRLYGSFIEHLGRAVYGGIYEPGHA-TADENGFRQDVLNAVRALNVPI 66 + K + ++ E V + + Sbjct: 7 ALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLIDMGEEIGLATVYRVLNQFDDAG 66 Query: 67 IRYPGGNFVSGYNWEDGVGPKAERKQTLELAW-WTTETNHVGTNEFAEWAKLAGSEVMMA 125 I +E L + E + A + + Sbjct: 67 IVTRHNFEGGKSVFELTQQ---HHHDHLICLDCGKVIEFSDDSIEARQREIAAKHGIRLT 123 Query: 126 VNLGTRGIDAARNLVEYCNHPSGSYWSDLRISHGVKAPHQFKTWCLGNEMDGPWQIGAKT 185 Sbjct: 124 --- 180 Query: 186 AVEYGRLANETAKAMRWV-DPSLELVACGSSGSGMSTFAEWEATVLDLTYDNVDFLSLHT 244 Sbjct: 181 --- 237 Query: 245 YYNNND-----SDTANF--------LAKSLDLDQFIDSVAAICDXXXXXXXXXXXXXXXX 291 Sbjct: 238 ---- 293 Query: 292 DEWNVWKSQGSSRMETHWQIAPPEFEDVYNMEDALAVGCCLISLLKHADRVKMACLAQLI 351 Sbjct: 294 ------- 346 Query: 352 NTIAPITTENGGPLWLQTTYYPYMHASLYGRGTVLHPLITSPKYDSSDFTDVPYLEAVSV 411 Sbjct: 347 -- 404 Query: 412 YNEELSEITVFAVNRHLSEGMELSVDLRSFGPTEVIQHTVMEHEDLQAANTKLHPGNVLP 471 Sbjct: 405 464 Query: 472 HDRGTAVAEDGRITALLSKASWNVIRLRVKS 502 Sbjct: 465 495 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.133 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 36,721,234 Number of extensions: 1527341 Number of successful extensions: 2341 Number of sequences better than 1.0e-05: 4 Number of HSP's gapped: 2326 Number of HSP's successfully gapped: 4 Length of query: 502 Length of database: 38,378,431 Length adjustment: 111 Effective length of query: 391 Effective length of database: 25,576,357 Effective search space: 10000355587 Effective search space used: 10000355587 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 118 (50.1 bits)