BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0584.0027.p
         (323 letters)

Database: COG 
           115,334 sequences; 38,378,431 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

STM0077 [C] COG0644 Dehydrogenases (flavoproteins)                    149   9e-36
TM0077 [Q] COG3458 Acetyl esterase (deacetylase)                      149   9e-36
TM0435 [Q] COG3458 Acetyl esterase (deacetylase)                       62   9e-10

>STM0077 [C] COG0644 Dehydrogenases (flavoproteins)
          Length = 428

 Score =  149 bits (375), Expect = 9e-36,   Method: Compositional matrix adjust.
 Identities = 22/325 (6%), Positives = 58/325 (17%), Gaps = 14/325 (4%)

Query: 1   MNTIELRKQELENCRPEPTLEQGVVNEFWDGILAEYARKPLEIISVPEVTPYPGMRVDKV 60
           M+        +       ++   V+      +L           +V     Y       +
Sbjct: 1   MSEDIFDAIIVGAGLAG-SVAALVLAREGAQVLVIERGNSAGAKNVTGGRLYAHSLERII 59

Query: 61  SFQSFDETRVHAWHIQPAQAGNGGGPLPCIITFPGYTGDRGYPERYASWLLLGYSVLAVD 120
              +           +             +        D    +   S L   +    ++
Sbjct: 60  PGFADQAPIERMITHEKLAFMT---DNGAMTIDYCNGEDASASQVSYSVLRSKFDAWLME 116

Query: 121 VRGQ-----LGETGNLL--PMESGVAKGWITQGLLEKEHSYYMAVAMDTVRAVETAAQLQ 173
              +     +           +  V  G    G + +     +A  ++++ A +     +
Sbjct: 117 QAEEAGAQLITGIRVDNVVQRDGKVV-GVEADGDILEAKVVILADGVNSLLAEKLGMTKR 175

Query: 174 GVDPSRXXXXXXXXXXXXXXXXXXXXXXXXXVAADIPNLCRLDFGV-LNSTSSLTEIGAY 232
                                           AA +      D  +      +     + 
Sbjct: 176 VEASHVAVGVKELIELPKLVIEDRFQLQGNEGAACLFAGAPTDGLMGGGFLYTNETTLSL 235

Query: 233 VRRYPEHLERVLDTLAYFDIVNLAPRITAPVLISVGWKDTVCMPESVYAAYNRIESDKQI 292
                 H  +         + +         LI+ G            A  N        
Sbjct: 236 GLVCGLHHLKDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEYAAHVVPEAGMNMQPELVGD 295

Query: 293 MDYPFSGHE-VSEYHLRQKVLFLQK 316
                     +              
Sbjct: 296 GVLIAGDAAGMCMNLGFTIRGMDLA 320


>TM0077 [Q] COG3458 Acetyl esterase (deacetylase)
          Length = 325

 Score =  149 bits (375), Expect = 9e-36,   Method: Compositional matrix adjust.
 Identities = 101/325 (31%), Positives = 150/325 (46%), Gaps = 14/325 (4%)

Query: 1   MNTIELRKQELENCRPEPTLEQGVVNEFWDGILAEYARKPLEIISVPEVTPYPGMRVDKV 60
           M   +L  +EL+  RPE   E+   +EFW+  LAE  + PL+ +     +    +    V
Sbjct: 1   MAFFDLPLEELKKYRPE-RYEEKDFDEFWEETLAESEKFPLDPVFERMESHLKTVEAYDV 59

Query: 61  SFQSFDETRVHAWHIQPAQAGNGGGPLPCIITFPGYTGDRGYPERYASWLLLGYSVLAVD 120
           +F  +   R+  W + P         LPC++ + GY G RG+P  +  W  +GY    +D
Sbjct: 60  TFSGYRGQRIKGWLLVPKLEEE---KLPCVVQYIGYNGGRGFPHDWLFWPSMGYICFVMD 116

Query: 121 VRGQ-----LGETGNLL--PMESGVAKGWITQGLLEKEHSYYMAVAMDTVRAVETAAQLQ 173
            RGQ      G+T +    P++     G++T+G+L+    YY  V  D VRAVE AA   
Sbjct: 117 TRGQGSGWLKGDTPDYPEGPVDPQYP-GFMTRGILDPRTYYYRRVFTDAVRAVEAAASFP 175

Query: 174 GVDPSRXXXXXXXXXXXXXXXXXXXXXXXXXVAADIPNLCRLDFGV-LNSTSSLTEIGAY 232
            VD  R                         +  D+P LC     V L  T    EI  +
Sbjct: 176 QVDQERIVIAGGSQGGGIALAVSALSKKAKALLCDVPFLCHFRRAVQLVDTHPYAEITNF 235

Query: 233 VRRYPEHLERVLDTLAYFDIVNLAPRITAPVLISVGWKDTVCMPESVYAAYNRIESDKQI 292
           ++ + +  E V  TL+YFD VN A R   P L SVG  D +C P +V+AAYN     K+I
Sbjct: 236 LKTHRDKEEIVFRTLSYFDGVNFAARAKIPALFSVGLMDNICPPSTVFAAYNYYAGPKEI 295

Query: 293 MDYPFSGHE-VSEYHLRQKVLFLQK 316
             YP++ HE    +   ++V FL+K
Sbjct: 296 RIYPYNNHEGGGSFQAVEQVKFLKK 320


>TM0435 [Q] COG3458 Acetyl esterase (deacetylase)
          Length = 301

 Score = 62.4 bits (150), Expect = 9e-10,   Method: Compositional matrix adjust.
 Identities = 8/163 (4%), Positives = 25/163 (15%), Gaps = 11/163 (6%)

Query: 1   MNTIELRKQELENCRPEPTLEQGVVNEFWDGILAEYARKPLEIISVPEVTPYPGMRVDKV 60
           M+  +     +        + +  +     G       +  +      V  +       +
Sbjct: 1   MSIDKSYCGFIAIVGRP-NVGKSTLLNKLLGQKISITSRKAQTTRHRIVGIHTEGAYQAI 59

Query: 61  SFQSFDETRVHAWHIQPAQAGNGGGPLPCIITFPGYTGDRGYPERYASWLLLGYSVLAVD 120
              +          I                         G        ++L        
Sbjct: 60  YVDTPGLHMEEKRAINRLMNKAAS--SSIGDVELVIFVVEGTRWTPDDEMVLNKLREGKA 117

Query: 121 VRGQ-----LGETGNLL--PMESGVAKGWITQGLLEKEHSYYM 156
                              P    +A   +    +    +   
Sbjct: 118 PVILAVNKVDNVQEKADLLPHLQFLA-SQMNFLDIVPISAETG 159


  Database: COG
    Posted date:  Aug 3, 2010  9:58 AM
  Number of letters in database: 38,378,431
  Number of sequences in database:  115,334
  
Lambda     K      H
   0.319    0.137    0.417 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 115334
Number of Hits to DB: 24,215,283
Number of extensions: 993369
Number of successful extensions: 2009
Number of sequences better than 1.0e-05: 3
Number of HSP's gapped: 2003
Number of HSP's successfully gapped: 3
Length of query: 323
Length of database: 38,378,431
Length adjustment: 107
Effective length of query: 216
Effective length of database: 26,037,693
Effective search space: 5624141688
Effective search space used: 5624141688
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 116 (49.3 bits)