BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0584.0020.p (370 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value lin0696 [M] COG0463 Glycosyltransferases involved in cell wall b... 117 2e-26 RSp0387 [M] COG0463 Glycosyltransferases involved in cell wall b... 87 5e-17 HI0653 [M] COG0463 Glycosyltransferases involved in cell wall bi... 84 3e-16 PM1306 [M] COG0463 Glycosyltransferases involved in cell wall bi... 78 2e-14 mll0582_1 [M] COG0463 Glycosyltransferases involved in cell wall... 77 6e-14 >lin0696 [M] COG0463 Glycosyltransferases involved in cell wall biogenesis Length = 301 Score = 117 bits (294), Expect = 2e-26, Method: Compositional matrix adjust. Identities = 13/325 (4%), Positives = 42/325 (12%), Gaps = 74/325 (22%) Query: 4 ISLCMIVRNEENSLPRCLSSAVSIADEIVVVDTGSTDRTKEIAASFGAVIYDFTWIEDFS 63 S C + + + + + + + T+ D Sbjct: 5 KSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTRHRIVGIHTEGAYQAIYVDTP 64 Query: 64 AARNFAFSKATKDYILWLDADDYLKDKDQELFRGLKTSLPGHVDSVNMPYNLAFDA-AGN 122 + + + + ++ + D + + A Sbjct: 65 GLHMEEKRAINRLMNKAASSSIGD---VELVIFVVEGTRWTPDDEMVLNKLREGKAPVIL 121 Query: 123 VVTSLRRNRLVRRSCNFKWIGPVHEYLE---------------VYGPSYSSDVCITHEKD 167 V + + + TH Sbjct: 122 AVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGLNVDTIAAIVRKHLPEATHHF- 180 Query: 168 KAYTDRNLRIYQKRAAAGEAFSPRDQYYYANELRDHGMHEEACRYYTLFLEGGQGWIEDN 227 + + +R A E Sbjct: 181 ---PEDYITDRSQRFMASE---------------------------------IIREKLMR 204 Query: 228 YQACLRLAECQERLGNREEAFQALCRTLKIDKPRAEFCCRLGAWHLEKNQLHQAVYWYEL 287 + E + + + Sbjct: 205 ------FLGAELPYSVTVEIERFVSNERGGYDINGLILVEREGQKKM-----------VI 247 Query: 288 AVMLPRQNDSMGIRNESYSTWLPNL 312 + + Sbjct: 248 GNKGAKIKTIGIEARKDMQEM-FEA 271 >RSp0387 [M] COG0463 Glycosyltransferases involved in cell wall biogenesis Length = 362 Score = 86.7 bits (213), Expect = 5e-17, Method: Compositional matrix adjust. Identities = 43/92 (46%), Positives = 61/92 (66%) Query: 3 EISLCMIVRNEENSLPRCLSSAVSIADEIVVVDTGSTDRTKEIAASFGAVIYDFTWIEDF 62 +++L +I RNE + RCL SA D ++V+DTGSTD T IA + GA ++ FTW++DF Sbjct: 12 DLALVVIARNEAACIERCLRSARPHVDRMIVLDTGSTDATVAIAEACGAQVHHFTWVDDF 71 Query: 63 SAARNFAFSKATKDYILWLDADDYLKDKDQEL 94 SAARN A A D+ L +DAD++L+ EL Sbjct: 72 SAARNAALQFADADWNLVMDADEWLESGAGEL 103 >HI0653 [M] COG0463 Glycosyltransferases involved in cell wall biogenesis Length = 254 Score = 84.0 bits (206), Expect = 3e-16, Method: Compositional matrix adjust. Identities = 41/93 (44%), Positives = 57/93 (61%) Query: 1 MIEISLCMIVRNEENSLPRCLSSAVSIADEIVVVDTGSTDRTKEIAASFGAVIYDFTWIE 60 M IS+ MI++NE L CL + DEI+++D+GSTD TKEIA S+GA Y+ + + Sbjct: 1 MPTISVAMIIKNEAQDLANCLDTVKDWVDEIIILDSGSTDNTKEIALSYGAKFYENSDWQ 60 Query: 61 DFSAARNFAFSKATKDYILWLDADDYLKDKDQE 93 F R A T DY+LWLDAD+ + K Q+ Sbjct: 61 GFGKQRQLAQQYVTSDYVLWLDADERVTPKLQQ 93 >PM1306 [M] COG0463 Glycosyltransferases involved in cell wall biogenesis Length = 254 Score = 78.2 bits (191), Expect = 2e-14, Method: Compositional matrix adjust. Identities = 39/85 (45%), Positives = 51/85 (60%) Query: 1 MIEISLCMIVRNEENSLPRCLSSAVSIADEIVVVDTGSTDRTKEIAASFGAVIYDFTWIE 60 M +S+ MIV+NE L +CL + D+IV++D+GSTD TKEIA S+GA Y Sbjct: 1 MPTLSVAMIVKNEAEHLAQCLDTVKDWVDDIVILDSGSTDNTKEIALSYGAKFYAHCEWP 60 Query: 61 DFSAARNFAFSKATKDYILWLDADD 85 F R A T DY+LWLDAD+ Sbjct: 61 GFGRQRQIAQQYVTTDYVLWLDADE 85 >mll0582_1 [M] COG0463 Glycosyltransferases involved in cell wall biogenesis Length = 301 Score = 76.6 bits (187), Expect = 6e-14, Method: Compositional matrix adjust. Identities = 11/243 (4%), Positives = 34/243 (13%), Gaps = 48/243 (19%) Query: 1 MIEISLCMIVRNEENSLPRCLSSAVSIADEIVVVDTGSTDRTKEIAASF-------GAVI 53 M S+ ++ + T +T A+ Sbjct: 1 MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTRHRIVGIHTEGAYQAIY 60 Query: 54 YDFTWIEDFSAARNFAFSKATK----DYILWLDADDYL-KDKDQELFRGLKTSLPGHVDS 108 T + K + + + + + Sbjct: 61 -VDTP-GLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWTPDDE-MVLNKLREGKA 117 Query: 109 VNMPYNLAFDAAGNVVTSLRRNRLVRRSCNFKWIGPVHEYLEVYGPSYSSDVCITHEKDK 168 + N + +++ +L++ P + Sbjct: 118 PVILA-------VNK--VDNVQEKADLLPHLQFLASQMNFLDI-VPIS-----AETGLNV 162 Query: 169 AY---------TDRNLRIYQKRAAAGEA---------FSPRDQYYYANELRDHGMHEEAC 210 + + E Sbjct: 163 DTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEIERFV 222 Query: 211 RYY 213 Sbjct: 223 SNE 225 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.321 0.135 0.421 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 27,874,218 Number of extensions: 1117800 Number of successful extensions: 2006 Number of sequences better than 1.0e-05: 18 Number of HSP's gapped: 2030 Number of HSP's successfully gapped: 18 Length of query: 370 Length of database: 38,378,431 Length adjustment: 108 Effective length of query: 262 Effective length of database: 25,922,359 Effective search space: 6791658058 Effective search space used: 6791658058 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 116 (49.3 bits)