BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0584.0017.p (270 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value lin0667 [H] COG0351 Hydroxymethylpyrimidine/phosphomethylpyrimid... 357 1e-98 BS_thiD [H] COG0351 Hydroxymethylpyrimidine/phosphomethylpyrimid... 281 8e-76 SA0537 [H] COG0351 Hydroxymethylpyrimidine/phosphomethylpyrimidi... 258 5e-69 Cgl3018 [H] COG0351 Hydroxymethylpyrimidine/phosphomethylpyrimid... 205 7e-53 lin0342 [H] COG0351 Hydroxymethylpyrimidine/phosphomethylpyrimid... 152 7e-37 >lin0667 [H] COG0351 Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase Length = 182 Score = 357 bits (915), Expect = 1e-98, Method: Compositional matrix adjust. Identities = 0/243 (0%), Positives = 0/243 (0%) Query: 25 LKTFEEYGTYGFSALTTIVTMDPDNGWHHNVYPIDAAIVAEQLKTVFAGGPVDAMKTGML 84 T + FS+ + + G + Sbjct: 24 AHTADTRLHLAFSSWLFNAKGQLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRR 83 Query: 85 GSVDIVKVAEKAIKENLQTNVVIDPVMVCKGEDEVLNPESANAIRDLLLPLATVATPNLF 144 ++ + + + + Sbjct: 84 CRYELGVEITPPESIYPDFRYRATDPSGIVENEVCPVFAARTTSALQINDDEVMDYQWCD 143 Query: 145 EAGVLSGLGKLTSIDEMKEAARLIHALGTQNVVVKGGKALGGDLAIDVFFDGSEYTVLET 204 Sbjct: 144 203 Query: 205 AKIEPAYNHGAGCTFAAAITGGLANGLSVHDAVVKAKDFVSAAIRSGYAFNQYVGPVFHG 264 Sbjct: 204 263 Query: 265 GYR 267 Sbjct: 264 266 >BS_thiD [H] COG0351 Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase Length = 266 Score = 281 bits (719), Expect = 8e-76, Method: Compositional matrix adjust. Identities = 0/265 (0%), Positives = 0/265 (0%) Query: 6 KALTXXXXXXXXXXXXXXXLKTFEEYGTYGFSALTTIVTMDPDNGWHHNVYPIDAAIVAE 65 ALT LKTF G YG S +T +V + D Sbjct: 5 NALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQNTRGVQSVYRIEPDFVAAQL 64 Query: 66 QLKTVFAGGPVDAMKTGMLGSVDIVKVAEKAIKENLQTNVVIDPVMVCKGEDEVLNPESA 125 + + + + + + + Sbjct: 65 DSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNVVLDTVMLAKSGDPLLSPSAVAT 124 Query: 126 NAIRDLLLPLATVATPNLFEAGVLSGLGKLTSIDEMKEAARLIHALGTQNVVVKGGKALG 185 R L A + + + + + L G + Sbjct: 125 LRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLEQGRSLLAMGCGAVLMKGGHLDDEQ 184 Query: 186 GDLAIDVFFDGSEYTVLETAKIEPAYNHGAGCTFAAAITGGLANGLSVHDAVVKAKDFVS 245 + +T AA+ N Sbjct: 185 SPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAALAALRPRHTNWADTVQEAKSWLSSAL 244 Query: 246 AAIRSGYAFNQYVGPVFHGGYRLER 270 Sbjct: 245 269 >SA0537 [H] COG0351 Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase Length = 276 Score = 258 bits (660), Expect = 5e-69, Method: Compositional matrix adjust. Identities = 134/247 (54%), Positives = 174/247 (70%), Gaps = 3/247 (1%) Query: 25 LKTFEEYGTYGFSALTTIVTMDPDNGWHHNVYPIDAAIVAEQLKTVFAGGPVDAMKTGML 84 LKTF+E TYG ALT IVTMD D W H+V P+ + +QL+T + GP DA+KTGML Sbjct: 24 LKTFQELDTYGMVALTAIVTMDKDT-WSHDVTPLPMDVFEKQLETALSIGP-DAIKTGML 81 Query: 85 GSVDIVKVAEKAIKENLQTNVVIDPVMVCKGEDEVLNPESANAIRDLLLPLATVATPNLF 144 G+ +I+K A + + + V+DPVMVCKGEDEVLNP + A+ LLP ATV TPNLF Sbjct: 82 GTEEIIKRAGEVYEASNAQYFVVDPVMVCKGEDEVLNPGNTEAMIKYLLPKATVVTPNLF 141 Query: 145 EAGVLSGLGKLTSIDEMKEAARLIHALGTQNVVVKGGKALGGDLAIDVFFDGSEYTVLET 204 EAG LSGLGKL SI++MK+AA +I G Q+V++KGGKAL D + D+++DG + L T Sbjct: 142 EAGQLSGLGKLNSIEDMKKAATIIFDKGAQHVIIKGGKALDQDKSYDLYYDGQTFYQLTT 201 Query: 205 AKIEPAYNHGAGCTFAAAITGGLANGLSVHDAVVKAKDFVSAAIRSGYAFNQYVGPVFHG 264 + +YNHGAGCTFAAA T LANG S +AV+ AK FV++AI++G+ N +VGPV HG Sbjct: 202 DMFQQSYNHGAGCTFAAATTAYLANGKSPKEAVISAKAFVASAIKNGWKMNDFVGPVDHG 261 Query: 265 GY-RLER 270 Y R+E Sbjct: 262 AYNRIEH 268 >Cgl3018 [H] COG0351 Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase Length = 266 Score = 205 bits (521), Expect = 7e-53, Method: Compositional matrix adjust. Identities = 108/231 (46%), Positives = 143/231 (61%) Query: 25 LKTFEEYGTYGFSALTTIVTMDPDNGWHHNVYPIDAAIVAEQLKTVFAGGPVDAMKTGML 84 LKTF+ YG A+T +V DP + W+H P+DA ++A Q++ A +D +K GML Sbjct: 23 LKTFQHLDVYGIGAITCLVAFDPKDNWNHRFVPVDAQVIANQIEAATAAHDLDVVKIGML 82 Query: 85 GSVDIVKVAEKAIKENLQTNVVIDPVMVCKGEDEVLNPESANAIRDLLLPLATVATPNLF 144 G+ + A++EN +VV+DPV++CKG++ ++ A+R +LP ATV TPN F Sbjct: 83 GTPATIDTVATALEENSFKHVVLDPVLICKGQEPGAALDTDTALRAKVLPQATVVTPNNF 142 Query: 145 EAGVLSGLGKLTSIDEMKEAARLIHALGTQNVVVKGGKALGGDLAIDVFFDGSEYTVLET 204 EA LSGL KL +ID++KEAARLIH G Q VVVKGG GD A+DV FDG++Y V Sbjct: 143 EATTLSGLDKLETIDDLKEAARLIHEQGPQYVVVKGGIDFPGDNAVDVLFDGTDYHVFSE 202 Query: 205 AKIEPAYNHGAGCTFAAAITGGLANGLSVHDAVVKAKDFVSAAIRSGYAFN 255 KI GAGCTFAA IT LA G S DAV AK V+ A++ A N Sbjct: 203 PKIGDERVSGAGCTFAAVITAELAKGNSAVDAVTTAKRVVTRAVKDAVASN 253 >lin0342 [H] COG0351 Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase Length = 182 Score = 152 bits (383), Expect = 7e-37, Method: Compositional matrix adjust. Identities = 0/245 (0%), Positives = 0/245 (0%), Gaps = 4/245 (1%) Query: 25 LKTFEEYGTYGFSALTTIVTMDPDNGWHHNVYPIDAAIVAEQLKTVFAGGPVDAMKTGML 84 T + FS+ A ++ + ++ Sbjct: 24 AHTADTRLHLAFSSWLFNAKGQLLVT--RRALSKKAWPGVWTNSVCGHPQLGESNEDAVI 81 Query: 85 GSVDIVKVAEKAIKENLQTNVVIDPVMVCKGEDEVLNPESANAIRDLLLPLATVATPNLF 144 E E++ + + + P A L + Sbjct: 82 RRCRYELGVEITPPESIYPDFRYRATDPSGIVENEVCPVFAARTTSALQINDDEVMDYQW 141 Query: 145 EAGVLSGLGKLTSIDEMKEAARLIHALGTQNVVVKGGKALGGDLAIDVFFDGSEYTVLET 204 Sbjct: 142 CDLADVL-- 199 Query: 205 AKIEPAYNHGAGCTFAAAITGGLANGLSVHDAVVKAKDFVSAAIRSGYAFNQYVGPVFHG 264 Sbjct: 200 259 Query: 265 GYRLE 269 Sbjct: 260 264 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.136 0.388 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 18,794,835 Number of extensions: 727921 Number of successful extensions: 1625 Number of sequences better than 1.0e-05: 27 Number of HSP's gapped: 1600 Number of HSP's successfully gapped: 27 Length of query: 270 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 165 Effective length of database: 26,268,361 Effective search space: 4334279565 Effective search space used: 4334279565 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 115 (48.9 bits)