BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0584.0015.p (314 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSp0222 [PR] COG4638 Phenylpropionate dioxygenase and related ri... 107 3e-23 CC2393 [PR] COG4638 Phenylpropionate dioxygenase and related rin... 103 3e-22 Cgl2332 [PR] COG4638 Phenylpropionate dioxygenase and related ri... 94 2e-19 SMa1501 [PR] COG4638 Phenylpropionate dioxygenase and related ri... 93 7e-19 PA4904 [PR] COG4638 Phenylpropionate dioxygenase and related rin... 92 1e-18 >RSp0222 [PR] COG4638 Phenylpropionate dioxygenase and related ring-hydroxylating dioxygenases, large terminal subunit Length = 342 Score = 107 bits (266), Expect = 3e-23, Method: Compositional matrix adjust. Identities = 91/331 (27%), Positives = 142/331 (42%), Gaps = 37/331 (11%) Query: 5 DEVQDKPLAVKLLDMKLVCYRS-EGKVVIARDLCFHRGAPLSKGWVENGDIVCPYHGFRY 63 DE+ DKPL ++ ++V YR EGKV D C HRGAPLS G+V +G +VC YHG Sbjct: 14 DEIADKPLGRRICGERMVFYRGQEGKVAALEDFCPHRGAPLSLGFVRDGHLVCGYHGLTM 73 Query: 64 NCEGKCTAVPAHPSSKISPKLKLIVYPAVERYGLIWTCLGSAAEQIP---DFPGWEDPDY 120 +GKC ++P ++ + +P VERYG IW G + P W + + Sbjct: 74 KADGKCASMPGQ---RVGGFPCIRQFPVVERYGFIWVWPGDPEQADPARIHHLEWAESEA 130 Query: 121 INILPPGFDIAGSSGRQMEGFLDVSHFAYVHTATFGDRNNTE-VPQYKVRREGNELVAE- 178 + I ++ +D++H YVH + G E PQ KV EG+ +V Sbjct: 131 WAYGGGLYHIQCDYRLMIDNLMDLTHETYVHATSIGQPEIEEAAPQTKV--EGDAVVTSR 188 Query: 179 ---------YWSTVSNYGKGQENPAPEGFMWLREFRVFPPFAASLTVYFPDEGRLN---- 225 +W+T G G + P + F PP + V G+ Sbjct: 189 FMENIMPPPFWATALR-GAGLADDVPCDRWQICRFT--PPSHVLIEVGVAHAGKGGYHAA 245 Query: 226 --------ILNCASPVSARYTRLFCPITRNFD-KSAPVEDTIKFNL-QVFAEDADMVEAQ 275 +++ +P + F + RNF A + DTI+ ++F+ED DM+E+Q Sbjct: 246 PEHRVGSIVVDFITPETETSIWYFWGMARNFRVDDAALTDTIRQGQGKIFSEDLDMLESQ 305 Query: 276 TPEDLPLDLQAEAHIPADRTSIAYRQLLTEL 306 L + + D + R++L L Sbjct: 306 QRNLLAYPERNLLKLNIDAGGVQSRRVLERL 336 >CC2393 [PR] COG4638 Phenylpropionate dioxygenase and related ring-hydroxylating dioxygenases, large terminal subunit Length = 353 Score = 103 bits (258), Expect = 3e-22, Method: Compositional matrix adjust. Identities = 82/302 (27%), Positives = 134/302 (44%), Gaps = 41/302 (13%) Query: 5 DEVQDKPLAVKLLDMKLVCYRS-EGKVVIARDLCFHRGAPLSKGWVENGDIVCPYHGFRY 63 DE+ D+PL ++ +V +R + + V D C HRGAPLS G+V +G++VC YHG Sbjct: 19 DEIADRPLGRRICGKAMVFHRGKDDRPVALEDFCPHRGAPLSLGFVRDGELVCGYHGLTM 78 Query: 64 NCEGKCTAVPAHPSSKISPKLKLIVYPAVERYGLIWTCLGSAAEQIP---------DFPG 114 CEG+ TA+P +++ + +P +ERYG IW G A+ P D P Sbjct: 79 GCEGRATAMPGQ---RVAAFPAIRAFPVLERYGFIWVWPGDPAQADPAKLHPLFWADSPD 135 Query: 115 WEDPDYINILPPGFDIAGSSGRQMEGFLDVSHFAYVHTATFGDRNNTEVPQYKVRREGNE 174 W Y L + IA ++ +D++H YVH + G + E P R EG E Sbjct: 136 WA---YGGGL---YHIACDYRLMVDNLMDLTHETYVHATSIGQKEIDEAP-VTTRLEGEE 188 Query: 175 LVAE----------YWSTVSNYGKGQENPAPEGFMWLR-------EFRVFPPFAASLTVY 217 +V +W EN + + R V A + Sbjct: 189 VVTSRFMEGIHAPPFWRMALRGAGLPENALVDRWQICRFSLPSHVMIEVGVALAGHGGYH 248 Query: 218 FPDEGRLN--ILNCASPVSARYTRLFCPITRNFD-KSAPVEDTIKFNL-QVFAEDADMVE 273 P + +++ +++ +P + F + R+F A + DTI+ ++F+ED +M+E Sbjct: 249 APADKKVSSVVVDFITPETETSHHYFWGMARSFAVGDAALTDTIREGQGKIFSEDLEMLE 308 Query: 274 AQ 275 Q Sbjct: 309 RQ 310 >Cgl2332 [PR] COG4638 Phenylpropionate dioxygenase and related ring-hydroxylating dioxygenases, large terminal subunit Length = 376 Score = 94.4 bits (233), Expect = 2e-19, Method: Compositional matrix adjust. Identities = 80/295 (27%), Positives = 130/295 (44%), Gaps = 35/295 (11%) Query: 10 KPLAVKLLDMKLVCYRS-EGKVVIARDLCFHRGAPLSKGWVENGD-IVCPYHGFRYNCEG 67 KP+A + + L YR+ +G+ V D C+HR APLSKG + D I CPYHG YN G Sbjct: 35 KPMARTIANKPLALYRTKDGRAVALADACWHRLAPLSKGKLVGTDGIQCPYHGLEYNSAG 94 Query: 68 KCTAVPAHPSSKISPKLKLIVYPAVERYGLIWTCLGSAA----EQIPDFPGWEDPDYINI 123 +C +PA + ++P + YP VE + +W LG Q+PD P++ Sbjct: 95 RCMKMPAQET--LNPSAAVNSYPVVEAHRFVWVWLGDPTLADPTQVPDMHQMSHPEWAG- 151 Query: 124 LPPGFDIAGSSGRQ--MEGFLDVSHFAYVHTATFGDRNNTEVPQYKVRREGNELVAEYWS 181 G I+ Q ++ +D++H +VH+++ G +E E + V + Sbjct: 152 --DGRTISADCNYQLVLDNLMDLTHEEFVHSSSIGQDELSESEFVVTHTEDSVTVTRWMH 209 Query: 182 TVSNYGKGQEN-----PAPEGFM--WLREFRVFPPFAASLTVYFPDEGR----------L 224 + Q+N P EG + W + + P + V G + Sbjct: 210 DIDAPPFWQKNMNDKFPGFEGKVDRW-QIIHYYYPSTICIDVGVAKAGTGAQEGDRSQGV 268 Query: 225 N--ILNCASPVSARYTRLFCPITRNFD-KSAPVEDTIKFNLQ-VFAEDADMVEAQ 275 N ++N +P S R + F RN+ +S + ++ + VF ED DM+ AQ Sbjct: 269 NGYVMNTITPDSDRSSHYFWAFMRNYRLESQTITTQLRDGVSGVFKEDEDMLTAQ 323 >SMa1501 [PR] COG4638 Phenylpropionate dioxygenase and related ring-hydroxylating dioxygenases, large terminal subunit Length = 304 Score = 92.8 bits (229), Expect = 7e-19, Method: Compositional matrix adjust. Identities = 77/258 (29%), Positives = 123/258 (47%), Gaps = 22/258 (8%) Query: 64 NCEGKCTAVPAHPSSKISPKLKLIVY----PAVERYGLIWTCLGSAAEQIPDFPGWEDPD 119 + EGK A + P ++ + P YG IWT LG+ ++ P + + D Sbjct: 47 DGEGKAAAWHSRPELPAGSRVDIDTLDGGLPVKMAYGYIWTSLGTPPAELFAIPEYAESD 106 Query: 120 YINILPPGFDIAGSSGRQMEGFLDVSHFAYVHTATFGDRNNTEVPQYKVRR--EGNELVA 177 + + S+ R +E FLD+ HF YVHT G +TEV +Y V E +E+VA Sbjct: 107 RRRLNAASIGVNVSAPRAIENFLDMGHFPYVHTDILGAEPHTEVKEYDVELSVERDEIVA 166 Query: 178 EYWSTVSNYGKGQENPAPEGFMWLRE-FRVFPPFAASLTVYFP-DEGRLNILNC-ASPVS 234 T + + + A G + +RV P+ + L P DE RL+++ PV Sbjct: 167 ----TRCRFFQPMASTASTGGADVEYIYRVPHPYCSVLYKSSPVDESRLDVIAVFLQPVD 222 Query: 235 ARYTR---LFCPI-TRNFDKSAPVEDTIKFNLQVFAEDADMVEAQTPEDLPLDLQAEAHI 290 + R + C + N DK +F +F +D ++E Q P+ LPLD +AE I Sbjct: 223 QEHVRAHMMLCVLDEENEDKVIK-----RFQQTIFGQDKPILENQFPKRLPLDPRAETPI 277 Query: 291 PADRTSIAYRQLLTELGL 308 AD+++IAYR+ L++ G+ Sbjct: 278 RADKSAIAYRRWLSQKGV 295 >PA4904 [PR] COG4638 Phenylpropionate dioxygenase and related ring-hydroxylating dioxygenases, large terminal subunit Length = 351 Score = 91.7 bits (226), Expect = 1e-18, Method: Compositional matrix adjust. Identities = 80/302 (26%), Positives = 128/302 (42%), Gaps = 41/302 (13%) Query: 5 DEVQDKPLAVKLLDMKLVCYRSE-GKVVIARDLCFHRGAPLSKGWVENGDIVCPYHGFRY 63 DE+ KPL ++ D +V YR E G+V D C HRGAPLS G+VE+G +VC YHG Sbjct: 14 DELAGKPLGRRICDEPMVFYRGEDGRVAALEDFCPHRGAPLSLGFVEDGVLVCGYHGLAM 73 Query: 64 NCEGKCTAVPAHPSSKISPKLKLIVYPAVERYGLIWTCLGSAAEQ----IPDFPGWEDPD 119 +G+ A+P ++ + +P ER+G +W G+ + IP E PD Sbjct: 74 GEDGRTRAMPG---QRVRGFPCIRRFPVQERHGFVWVWPGAEEQADDALIPRLEWAESPD 130 Query: 120 --YINILPPGFDIAGSSGRQMEGFLDVSHFAYVHTATFGDRNNTEVPQYKVRREGNELVA 177 Y L + I ++ +D++H YVH ++ G + E R EG+E++ Sbjct: 131 WAYGGGL---YHIHCDYRLMIDNLMDLTHETYVHASSIGQKEIDEAAP-TTRVEGDEVIT 186 Query: 178 E----------YWSTVSNYGKGQENPAPEGFMWLREFRVFPPFAASLTVYFPDEGRLN-- 225 +W ++ A + + R PP + V G Sbjct: 187 SRHMQNVMAPPFWRMALRGNGLADDVAVDRWQIC---RFTPPSHVLIEVGVAHAGHGGYQ 243 Query: 226 ----------ILNCASPVSARYTRLFCPITRNFD--KSAPVEDTIKFNLQVFAEDADMVE 273 +++ +P SA F + RNF A E + ++F ED +M+E Sbjct: 244 APAERKAASIVVDFITPESATSIWYFWGMARNFQAHDQALTESIREGQGKIFGEDLEMLE 303 Query: 274 AQ 275 +Q Sbjct: 304 SQ 305 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.138 0.433 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 25,474,438 Number of extensions: 1094364 Number of successful extensions: 1485 Number of sequences better than 1.0e-05: 26 Number of HSP's gapped: 1455 Number of HSP's successfully gapped: 26 Length of query: 314 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 208 Effective length of database: 26,153,027 Effective search space: 5439829616 Effective search space used: 5439829616 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 116 (49.3 bits)