BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0584.0012.p (462 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value MA1208 [C] COG2710 Nitrogenase molybdenum-iron protein, alpha an... 744 0.0 MA1218 [C] COG2710 Nitrogenase molybdenum-iron protein, alpha an... 634 0.0 MTH1564 [C] COG2710 Nitrogenase molybdenum-iron protein, alpha a... 303 5e-82 all1440 [C] COG2710 Nitrogenase molybdenum-iron protein, alpha a... 247 3e-65 CAC0257 [C] COG2710 Nitrogenase molybdenum-iron protein, alpha a... 239 6e-63 >MA1208 [C] COG2710 Nitrogenase molybdenum-iron protein, alpha and beta chains Length = 462 Score = 744 bits (1922), Expect = 0.0, Method: Compositional matrix adjust. Identities = 356/462 (77%), Positives = 408/462 (88%) Query: 1 MTCEIVQKERGGVINPIFTCQPAGAQYASIGIKDCIGIVHGGQGCVMFVRLLFSQHFKDN 60 M+CE+ ++ER G+INPIFTCQP GAQYASIGIKDCI IVHGGQGCVMFVR+L +QHFKD+ Sbjct: 1 MSCELKERERPGIINPIFTCQPCGAQYASIGIKDCIAIVHGGQGCVMFVRMLLAQHFKDS 60 Query: 61 FLIASSSVHEDGAVFGALNRVEEAVDVLLMRYPDVKVVPIITTCSTEVIGDDVDGVILKL 120 F IASSS+HEDGAVFGALNRVEEAVDVLL RYP VKVVPIITTCSTEVIGDD+DG+I KL Sbjct: 61 FEIASSSLHEDGAVFGALNRVEEAVDVLLTRYPRVKVVPIITTCSTEVIGDDIDGLIRKL 120 Query: 121 NNGLLKEKYAGREVHLIAVHTPSFVGSQVSGYDVAVEAFVKHFARKGQPNGKLNLITGWV 180 N LLKEKYA REVHLI +HTPSF GS V+GYDVAV VK FA+K +PNGK+NLITGWV Sbjct: 121 NKKLLKEKYADREVHLIPIHTPSFKGSMVTGYDVAVHDIVKEFAKKDKPNGKINLITGWV 180 Query: 181 NPGDVTALKHLLAEMQVDATVLFEIESFDSPILPDKSGESHGSTTIADMTGXXXXXXXXX 240 NPGDVTALKHLL+ M +DATVLFEIESFDSP++PDKSG +HGSTTI D+TG Sbjct: 181 NPGDVTALKHLLSAMDIDATVLFEIESFDSPLMPDKSGVAHGSTTIEDLTGTANATGTIA 240 Query: 241 XXKYEGAKAAKYLEDEFGIPAVIGPTPIGIRNTDTFLQNVRKLTGKPIPESLVRERGIAI 300 +YEGAKAAKYLE EF +PA+IGPTPIGIRNTDTFLQN++K+TGKPIPESLV ERGIAI Sbjct: 241 LNRYEGAKAAKYLESEFDVPAIIGPTPIGIRNTDTFLQNLKKMTGKPIPESLVCERGIAI 300 Query: 301 DALADLVHMFLADKKVAIYGHPDLVIGLAEFCIDLEMQPVLLLLGDDNTTYKSDPRIQAL 360 DA+ DL HMF ADKKVAIYG+PDLVIGLAEFC+D+EM+P++LLLGDDN TY DPRI+ L Sbjct: 301 DAITDLTHMFFADKKVAIYGNPDLVIGLAEFCLDMEMKPMVLLLGDDNKTYPDDPRIKEL 360 Query: 361 QQNVEFGMEIVLNADLLELENRIKNKGLELDLILGHSKGRFTAIDNNIPMVRVGFPTYDR 420 Q+ V+F MEI++NAD+ ELE RIK+K +ELDLI+GHSKGR+TAIDNNIPMVRVGFPT+DR Sbjct: 361 QEKVDFDMEIIMNADMWELERRIKDKEIELDLIMGHSKGRWTAIDNNIPMVRVGFPTFDR 420 Query: 421 AGLYRHPTVGYAGAIRLAEEIANTLFTDMEYKKNKEWILSVW 462 AG+YRHP VGY GAI LAE++ANTLF DMEYKK++EWIL+VW Sbjct: 421 AGMYRHPVVGYEGAIWLAEQMANTLFADMEYKKDREWILNVW 462 >MA1218 [C] COG2710 Nitrogenase molybdenum-iron protein, alpha and beta chains Length = 461 Score = 634 bits (1634), Expect = 0.0, Method: Compositional matrix adjust. Identities = 284/462 (61%), Positives = 375/462 (81%), Gaps = 1/462 (0%) Query: 1 MTCEIVQKERGGVINPIFTCQPAGAQYASIGIKDCIGIVHGGQGCVMFVRLLFSQHFKDN 60 M+CE++ KER G+INP++TCQPAGAQYA IG+KDCI +VHGGQGC MFVRLLF+QHFK+N Sbjct: 1 MSCELMLKERTGIINPMYTCQPAGAQYAGIGVKDCIPLVHGGQGCSMFVRLLFAQHFKEN 60 Query: 61 FLIASSSVHEDGAVFGALNRVEEAVDVLLMRYPDVKVVPIITTCSTEVIGDDVDGVILKL 120 F IASSS+HE AVFG RVEEAV+ L+ RYP ++++PIITTCSTE IGDD++G+I K+ Sbjct: 61 FDIASSSLHESAAVFGGAIRVEEAVETLIARYPHLRIIPIITTCSTETIGDDIEGIIRKV 120 Query: 121 NNGLLKEKYAGREVHLIAVHTPSFVGSQVSGYDVAVEAFVKHFARKGQPNGKLNLITGWV 180 N + KE + R+V L+A+HTPS+ GS V+GYD A+ A VK A+KG+P+ KLN+ TGWV Sbjct: 121 NQKI-KENHPDRDVKLVAIHTPSYSGSMVTGYDNAISAMVKALAKKGEPSEKLNIFTGWV 179 Query: 181 NPGDVTALKHLLAEMQVDATVLFEIESFDSPILPDKSGESHGSTTIADMTGXXXXXXXXX 240 NPGDV+ +KH+L+EMQVD +L + E+FDSPI+PDKS ++G+TTI D+ Sbjct: 180 NPGDVSEIKHILSEMQVDGNILLDTETFDSPIMPDKSAFAYGNTTIEDIADSANALGSIA 239 Query: 241 XXKYEGAKAAKYLEDEFGIPAVIGPTPIGIRNTDTFLQNVRKLTGKPIPESLVRERGIAI 300 +YEGA AA +L+++F +P+++ PTPIGI NTD FL+N+ +LTGKPIPESLV ERG AI Sbjct: 240 LSRYEGANAATFLKEKFDVPSIVTPTPIGINNTDIFLKNISELTGKPIPESLVIERGKAI 299 Query: 301 DALADLVHMFLADKKVAIYGHPDLVIGLAEFCIDLEMQPVLLLLGDDNTTYKSDPRIQAL 360 D++ DL HMF A+KKVAI+G+PDLV GLA+FC++ E++PVLLLLGDDN++YK DPR++ L Sbjct: 300 DSIVDLAHMFFANKKVAIFGNPDLVFGLAQFCLECELEPVLLLLGDDNSSYKKDPRLKEL 359 Query: 361 QQNVEFGMEIVLNADLLELENRIKNKGLELDLILGHSKGRFTAIDNNIPMVRVGFPTYDR 420 ++ M+I+ NADL ELE+RIKNK +++DLILGHSKGR+ AIDN+IPMVRVGFPT+DR Sbjct: 360 EEKTNCDMQIIWNADLWELESRIKNKTIDVDLILGHSKGRYIAIDNDIPMVRVGFPTFDR 419 Query: 421 AGLYRHPTVGYAGAIRLAEEIANTLFTDMEYKKNKEWILSVW 462 AGL+++P +GY GA LAE IANT+F ME K ++EWI++VW Sbjct: 420 AGLWKYPVIGYKGAEWLAENIANTIFASMENKHDREWIINVW 461 >MTH1564 [C] COG2710 Nitrogenase molybdenum-iron protein, alpha and beta chains Length = 268 Score = 303 bits (775), Expect = 5e-82, Method: Compositional matrix adjust. Identities = 0/462 (0%), Positives = 0/462 (0%), Gaps = 17/462 (3%) Query: 5 IVQKERGGVINPIFTCQPAGAQYASIGIKDCIGIVHGGQGCVMFVRLLFSQHFKDNFLIA 64 + + + + I I V + Sbjct: 6 LSIFIPDSLTAETGDLKIKTYKVVLIARAASIFGVKRIVIYHDDADGEARFIRDILTYMD 65 Query: 65 SSSVHEDGAVFGALNRVEEAVDVLLMRYPDVKVVPIITTCSTEVIGDDVDGVILKLNNGL 124 + + L +T + + L + Sbjct: 66 TPQYLRRKVFPIMRELKHVGILPPLRTPHH-PTGKPVTGEYRQGLTVKRVK-KGTLVDIG 123 Query: 125 LKEKYAGR-EVHLIAVHTPSFVGSQVSGYDVAVEAFVKHFA-RKGQPNGKLNLITGWVNP 182 + R ++ + + + V E +++ L V Sbjct: 124 ADKLALCREKLTVNRIMSFRVVRLGKEILIEPDEPEDRYWGYEVLDTRRNLAESLKTVGA 183 Query: 183 GDVTALKHLLAEMQVDATVLFEI-ESFDSPILPDKSGE----SHGSTTIADMTGXXXXXX 237 V A + + + + + + Sbjct: 184 DVVVATSRNASPITSILDEVKTRMRGAREAAILFGGPYKGLPEIDADIWVNTLPGQCTET 243 Query: 238 XXXXXKYEGAKAAKYLEDEFGIPAVIGPTPIGIRNTDTFLQNVRKLTGKPIPESLVRERG 297 A + + Sbjct: 244 VRTEEAV-LATLSVFNMLTQIDEKD- 301 Query: 298 IAIDALADLVHMFLADKKVAIYGHPDLVIGLAEFCIDLEMQPVLLLLGDDNTTYKSDPRI 357 Sbjct: 302 -- 359 Query: 358 QALQQNVEFGMEIVLNADLLELENRIKNKGLELDLILGHSKGRFTAIDNNIPMVRVGFPT 417 Sbjct: 360 -- 417 Query: 418 YDRAGLYRHPTVGYAGAIRLAEEIANTLFTDMEYKKNKEWIL 459 Sbjct: 418 -- 457 >all1440 [C] COG2710 Nitrogenase molybdenum-iron protein, alpha and beta chains Length = 512 Score = 247 bits (631), Expect = 3e-65, Method: Compositional matrix adjust. Identities = 152/453 (33%), Positives = 240/453 (52%), Gaps = 27/453 (5%) Query: 13 VINPIFTCQPAGAQYASIGIKDCIGIVHGGQGCVMFVRLLFSQHFKDNFLIASSSVHEDG 72 +NP CQP GA +A++G + + V G QGCV + R S+H+K+ SSS+ ED Sbjct: 63 TVNPAKGCQPVGAMFAALGFEGTLPFVQGSQGCVAYFRTHLSRHYKEPCSAVSSSMTEDA 122 Query: 73 AVFGALNRVEEAVDVLLMRYPDVKVVPIITTCSTEVIGDDVDGVILKLNN-GLLKEKYAG 131 AVFG LN + E + V Y K++ + TTC EVIGDD+ I N G + + + Sbjct: 123 AVFGGLNNMIEGMQVSYQLY-KPKMIAVCTTCMAEVIGDDLGAFITNSKNAGSIPQDFP- 180 Query: 132 REVHLIAVHTPSFVGSQVSGYDVAVEAFVKHFA---RKGQPNGKLNLITGW-VNPGDVTA 187 + HTPSFVGS ++GYD ++ + + +K NGK+N I G+ G+ Sbjct: 181 ----VPFAHTPSFVGSHITGYDNMMKGILSNLTEGKKKATSNGKINFIPGFDTYVGNNRE 236 Query: 188 LKHLLAEMQVDATVLFE-IESFDSPILPDKSGESH---GSTTIADMTGXXXXXXXXXXXK 243 LK ++ M VD T+L + + FDSP + GE G T + D Sbjct: 237 LKRMMGVMGVDYTILSDSSDYFDSPNM----GEYEMYPGGTKLEDAADSINAKATVALQA 292 Query: 244 YEGAKAAKYLEDEFGIPAVIGPTPIGIRNTDTFLQNVRKLTGKPIPESLVRERGIAIDAL 303 Y K +Y++ ++ + P G++ TD FL V +LTGK IPE L ERG +DA+ Sbjct: 293 YTTPKTREYIKTQWKQETQV-LRPFGVKGTDEFLTAVSELTGKAIPEELEIERGRLVDAI 351 Query: 304 ADLVHMFLADKKVAIYGHPDLVIGLAEFCIDLEMQPVLLLLGDDNTTYKSDPRIQALQQN 363 D + ++ KK AIYG PDL+I + F +++ +PV +L + + T+K + ++A+ Sbjct: 352 TD-SYAWIHGKKFAIYGDPDLIISITSFLLEMGAEPVHILCNNGDDTFKKE--MEAILAA 408 Query: 364 VEFGME--IVLNADLLELENRIKNKGLELDLILGHSKGRFTAIDNNIPMVRVGFPTYDRA 421 FG E + + DL + + + +D +G+S G++ D +IPMVR+G+P +DR Sbjct: 409 SPFGKEAKVWIQKDLWHFRSLLFTE--PVDFFIGNSYGKYLWRDTSIPMVRIGYPLFDRH 466 Query: 422 GLYRHPTVGYAGAIRLAEEIANTLFTDMEYKKN 454 L+R+ T+GY G + + + NTL +M+ N Sbjct: 467 HLHRYSTLGYQGGLNILNWVVNTLLDEMDRSTN 499 >CAC0257 [C] COG2710 Nitrogenase molybdenum-iron protein, alpha and beta chains Length = 454 Score = 239 bits (610), Expect = 6e-63, Method: Compositional matrix adjust. Identities = 152/446 (34%), Positives = 231/446 (51%), Gaps = 17/446 (3%) Query: 14 INPIFTCQPAGAQYASIGIKDCIGIVHGGQGCVMFVRLLFSQHFKDNFLIASSSVHEDGA 73 INP TCQP GA YA++GI +C+ HG QGC + R+ ++HFKD + ++SS +E + Sbjct: 17 INPARTCQPIGAMYAALGIHNCLPHSHGSQGCCSYHRMQLTRHFKDPIMASTSSFNEGAS 76 Query: 74 VFGALNRVEEAVDVLLMRY-PDVKVVPIITTCSTEVIGDDVDGVILKLNNGLLKEKYAGR 132 VFG A+ + Y PD +V + TTC +E +GDD+ I E G+ Sbjct: 77 VFGGRANFNTAIKNIFSIYNPD--IVAVHTTCLSETLGDDLVSYIAD------SEVPEGK 128 Query: 133 EVHLIAVHTPSFVGSQVSGYDVAVEAFVKHFARKGQ-PNGKLNLITGWVNPGDVTALKHL 191 V I +TPS+VGS ++G+ V+A V + + + NGK+N++ G+V P D+ LK + Sbjct: 129 IV--IHANTPSYVGSHITGFSSMVKAMVTYLSENTKNKNGKVNVLPGFVGPADMRELKKI 186 Query: 192 LAEMQVDATVLFEIES-FDSPILPDKSGESHGSTTIADMTGXXXXXXXXXXXKYEGAKAA 250 M+V ++ + DSP+ + G TTI ++ AA Sbjct: 187 FKCMKVPFIMMPDTSGVVDSPMTGEYKMYPKGGTTIDELRDAGNSDYTFALGSLASEAAA 246 Query: 251 KYLEDEFGIPAVIGPTPIGIRNTDTFLQNVRKLTGKPIPESLVRERGIAIDALADLVHMF 310 LE + +P + TPIGI TD F+ + K+TGK +P + ERG +D + D + + Sbjct: 247 IELEKKCKVPFSVLNTPIGIDATDRFIMELSKITGKKVPYEIEEERGQLVDLMLD-SYQY 305 Query: 311 LADKKVAIYGHPDLVIGLAEFCIDLEMQPVLLLLGDDNTTYKSDPRIQALQQNVEFGMEI 370 KKVAI G PD VI L EF I L M P ++ G ++ + L+ N G ++ Sbjct: 306 FHGKKVAISGDPDTVIALTEFVISLGMNPKYVITGTKGEKFEKTIN-KMLEDNGIEGSKV 364 Query: 371 VLNADLLELENRIKNKGLELDLILGHSKGRFTAIDNNIPMVRVGFPTYDRAGLYRHPTVG 430 +DL EL IKN+ +DL++G+S +F A +IP+VR GFP DR G P VG Sbjct: 365 KSFSDLFELHQWIKNE--PVDLVIGNSHMKFIARAEDIPLVRFGFPVMDRYGHSYIPKVG 422 Query: 431 YAGAIRLAEEIANTLFTDMEYKKNKE 456 Y GA+ L + + + M+ +E Sbjct: 423 YKGAMNLLTSMCDAILERMDRDSAEE 448 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.140 0.411 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 35,652,204 Number of extensions: 1611964 Number of successful extensions: 6126 Number of sequences better than 1.0e-05: 20 Number of HSP's gapped: 6069 Number of HSP's successfully gapped: 20 Length of query: 462 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 352 Effective length of database: 25,691,691 Effective search space: 9043475232 Effective search space used: 9043475232 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 118 (50.1 bits)