BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0584.0005.p (439 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value all1437 [C] COG2710 Nitrogenase molybdenum-iron protein, alpha a... 261 1e-69 mlr5909 [C] COG2710 Nitrogenase molybdenum-iron protein, alpha a... 237 3e-62 SMa0873 [C] COG2710 Nitrogenase molybdenum-iron protein, alpha a... 231 2e-60 CAC0257 [C] COG2710 Nitrogenase molybdenum-iron protein, alpha a... 184 3e-46 MTH1564 [C] COG2710 Nitrogenase molybdenum-iron protein, alpha a... 182 9e-46 >all1437 [C] COG2710 Nitrogenase molybdenum-iron protein, alpha and beta chains Length = 444 Score = 261 bits (668), Expect = 1e-69, Method: Compositional matrix adjust. Identities = 149/421 (35%), Positives = 234/421 (55%), Gaps = 8/421 (1%) Query: 10 KPLSVNPFQTSQIIGAVLALQGIYRAVPILHGAQGCAESVKTVLSRHFREPISIQNIAIH 69 K ++VNP + SQ +GA LA G+ +P+ HG+QGC K VL RHFRE I + A+ Sbjct: 9 KSVAVNPLKQSQALGASLAFLGLKGMIPLFHGSQGCTAFAKVVLVRHFREAIPLATTAMT 68 Query: 70 EQNLIFGGEKGIRDMIRLVVEKYNPDVLALIGTSITEVVGEDLLGTAEGYYRQHKMMFKD 129 E I GGE I I +VEK +P+++ L T +TE G+D+ + +H + Sbjct: 69 EVTTILGGEDNIEQAILTLVEKSSPEIIGLCSTGLTETRGDDIERFLKDIRDRHPEISHL 128 Query: 130 KLMCGLYLPDYEGSMESGYAKSVYAVIQKVIQNSGXXXXXXXXXXXXLLPGAHLTPGDVM 189 ++ PD++G+++ G+A +V +++Q++ Q +L G+ TPGD+ Sbjct: 129 PIVFA-PTPDFKGALQDGFAAAVESIVQEIPQPG-----TTRSEQVTILAGSAFTPGDLQ 182 Query: 190 ELKEMIASFDIEVIVLPDLSSSLTGHLMTGHTPLSRGGVPLDYLREMLTSTYTLALGGSM 249 E+KE++ +F + I +PD+ +SL GHL ++ ++ G + L+E+ S +T+ALG SM Sbjct: 183 EIKEIVTAFGLVPIFVPDIGASLDGHLDEEYSSVTTSGTTVKQLQEVGCSAFTIALGESM 242 Query: 250 EACAKLLKEELQIPYQVFQGVTGLRETDEFFLFLRQFSQSDGQNKYRWQRQFLLDCMLDT 309 A++L++ IPY+VF +TGL DEF L S + KY QR+ L D MLDT Sbjct: 243 RGAARILEDRFNIPYEVFSELTGLEPVDEFIQALAILSSNSVPEKYCRQRRQLQDAMLDT 302 Query: 310 RSVFRGKRIIAALEPDHLIALYQCLAELGIKSVGSVIPAGSPAVAAMP-EAVQTGDLEDL 368 F KRI ALEPD L ++ + L +G + +V SP + +P ++V GDLED Sbjct: 303 HFYFGAKRISLALEPDLLWSMVKFLQSMGTQIHAAVTTTRSPLLEQLPIKSVTIGDLEDF 362 Query: 369 ERMAAGGADLWVSNAYGERHALKSGIPFLPLGFPVFNRFGAPANISVGYRGTADLLNNIA 428 E++A +DL + N+ A + IP LG P+++R G VGYRG+ ++L I Sbjct: 363 EQLAV-ESDLLIGNSNLAAIAKRLSIPHYRLGIPIYDRLGNGHYTKVGYRGSMEVLFGIG 421 Query: 429 N 429 N Sbjct: 422 N 422 >mlr5909 [C] COG2710 Nitrogenase molybdenum-iron protein, alpha and beta chains Length = 460 Score = 237 bits (604), Expect = 3e-62, Method: Compositional matrix adjust. Identities = 139/426 (32%), Positives = 224/426 (52%), Gaps = 6/426 (1%) Query: 10 KPLSVNPFQTSQIIGAVLALQGIYRAVPILHGAQGCAESVKTVLSRHFREPISIQNIAIH 69 K +VNP ++SQ +GA A G+ A+P+ HG+QGC + RHF+E I +Q A+ Sbjct: 9 KSAAVNPLKSSQPLGAAFAFLGVEGAMPLFHGSQGCTSFALVLFVRHFKEAIPLQTTAMD 68 Query: 70 EQNLIFGGEKGIRDMIRLVVEKYNPDVLALIGTSITEVVGEDLLGTAEGYYRQHKMMFKD 129 E I GG + + I + + P ++ + T++ E GED +H Sbjct: 69 EVATILGGADHLEEAILNLKTRTKPKLIGVCTTALVETRGEDCASDIANVKLKHVEELAG 128 Query: 130 KLMCGLYLPDYEGSMESGYAKSVYAVIQKVIQNSGXXXXXXXXXXXXLLPGAHLTPGDVM 189 + PD++G++E G+AK+V A+I+ + ++ +LPG +LT DV Sbjct: 129 TEVVLANTPDFDGAIEEGWAKAVAAMIEGITRSG---ERTRQPKKIAILPGCNLTVADVE 185 Query: 190 ELKEMIASFDIEVIVLPDLSSSLTGHLMTGHTPLSRGGVPLDYLREMLTSTYTLALGGSM 249 L++M+ SF ++ ++LPD+S SL G + + GG ++ +RE+ T+ + +G M Sbjct: 186 HLRDMVESFGLKPVILPDVSGSLDGTVPDRWVTTTYGGTSVEEIRELGTAAQCIVIGEHM 245 Query: 250 EACAKLLKEELQIPYQVFQGVTGLRETDEFFLFLRQFSQSDGQNKYRWQRQFLLDCMLDT 309 AK L +PY VFQ +TGL+ D F L S + ++ R R L D +LD Sbjct: 246 RHPAKTLHGLTGVPYAVFQSLTGLKAVDRFVSLLSAVSGAAVPDRVRRHRAQLEDALLDG 305 Query: 310 RSVFRGKRIIAALEPDHLIALYQCLAELGIKSVGSVIPAG-SPAVAAMP-EAVQTGDLED 367 F GK+I A EPD L L +G +V S + +P E VQ GDL D Sbjct: 306 HFHFGGKKIAIAAEPDQLYQLATFFTGMGCDIAAAVTTTDMSKILQKVPAEWVQIGDLGD 365 Query: 368 LERMAAGGADLWVSNAYGERHALKSGIPFLPLGFPVFNRFGAPANISVGYRGTADLLNNI 427 LE +AA GADL V++++G + + + IP + +GFP+F+R G+ +++ YRGT DL+ ++ Sbjct: 366 LEALAA-GADLLVTHSHGRQASRRLEIPLMRVGFPIFDRLGSQHKLTILYRGTRDLIFDV 424 Query: 428 ANTMNS 433 AN + Sbjct: 425 ANIFQA 430 >SMa0873 [C] COG2710 Nitrogenase molybdenum-iron protein, alpha and beta chains Length = 441 Score = 231 bits (589), Expect = 2e-60, Method: Compositional matrix adjust. Identities = 140/429 (32%), Positives = 230/429 (53%), Gaps = 11/429 (2%) Query: 10 KPLSVNPFQTSQIIGAVLALQGIYRAVPILHGAQGCAESVKTVLSRHFREPISIQNIAIH 69 K ++NP ++SQ +G LA G+ A+P+ HG+QGC +L RHF+E I +Q A+ Sbjct: 9 KWATINPLKSSQPLGGALAFLGVGGAIPLFHGSQGCTSFALVLLVRHFKEAIPLQTTAMD 68 Query: 70 EQNLIFGGEKGIRDMIRLVVEKYNPDVLALIGTSITEVVGEDLLGTAEGYY--RQHKMMF 127 + ++ GG + I + + P ++ + T++ E GEDL G R ++ Sbjct: 69 DVAIVLGGAGHLEQAILNLKIRAKPKLIGICTTALVETRGEDLAGDLASIKLERAEELTG 128 Query: 128 KDKLMCGLYLPDYEGSMESGYAKSVYAVIQKVIQNSGXXXXXXXXXXXXLLPGAHLTPGD 187 D ++ PD++G+ME G+AK+V A+I+ + + +LPG +LT D Sbjct: 129 TDVVLAN--TPDFDGAMEEGWAKAVTAMIKAITR---IGEQERQSRTIAILPGWNLTIAD 183 Query: 188 VMELKEMIASFDIEVIVLPDLSSSLTGHLMTGH-TPLSRGGVPLDYLREMLTSTYTLALG 246 + +L++++ SF ++ I+LPDLS SL G + P + GG+ ++ +RE+ T+ +A+G Sbjct: 184 IEQLRDIVESFGLKPIILPDLSGSLDGIVPDDRWVPTTYGGISVEEIRELGTAAQCIAIG 243 Query: 247 GSMEACAKLLKEELQIPYQVFQGVTGLRETDEFFLFLRQFSQSDGQNKYRWQRQFLLDCM 306 M A+ +K +PY +FQ +TGL D F L S K R +R L D + Sbjct: 244 EHMRGPAEEMKTLTGVPYVLFQSLTGLNAVDRFVSLLSSISGRPAPAKVRRRRAQLQDAL 303 Query: 307 LDTRSVFRGKRIIAALEPDHLIALYQCLAELGIKSVGSVIPAG-SPAVAAMP-EAVQTGD 364 LD GK+I A EPD L L LG + V +V G S + +P E +Q GD Sbjct: 304 LDGHFHSAGKKIAIAAEPDQLYQLATFFICLGAEIVAAVTTKGASKILHKVPVEIIQVGD 363 Query: 365 LEDLERMAAGGADLWVSNAYGERHALKSGIPFLPLGFPVFNRFGAPANISVGYRGTADLL 424 L DLE +A ADL V++++G+ + + G P + +GFPVF++ G+ +++ Y GT DL+ Sbjct: 364 LGDLESLAT-HADLLVTHSHGQHASARLGTPLMRVGFPVFDQLGSQHKLTILYHGTRDLI 422 Query: 425 NNIANTMNS 433 ++N S Sbjct: 423 FEVSNIFQS 431 >CAC0257 [C] COG2710 Nitrogenase molybdenum-iron protein, alpha and beta chains Length = 454 Score = 184 bits (466), Expect = 3e-46, Method: Compositional matrix adjust. Identities = 124/436 (28%), Positives = 215/436 (49%), Gaps = 25/436 (5%) Query: 10 KPLSVNPFQTSQIIGAVLALQGIYRAVPILHGAQGCAESVKTVLSRHFREPISIQNIAIH 69 K L +NP +T Q IGA+ A GI+ +P HG+QGC + L+RHF++PI + + Sbjct: 13 KALRINPARTCQPIGAMYAALGIHNCLPHSHGSQGCCSYHRMQLTRHFKDPIMASTSSFN 72 Query: 70 EQNLIFGGEKGIRDMIRLVVEKYNPDVLALIGTSITEVVGEDLLGTAEGYYRQHKMMFKD 129 E +FGG I+ + YNPD++A+ T ++E +G+DL+ Y + + Sbjct: 73 EGASVFGGRANFNTAIKNIFSIYNPDIVAVHTTCLSETLGDDLVS-----YIADSEVPEG 127 Query: 130 KLMCGLYLPDYEGSMESGYAKSVYAVIQKVIQNSGXXXXXXXXXXXXLLPGAHLTPGDVM 189 K++ P Y GS +G++ V A++ + +N+ +LPG + P D+ Sbjct: 128 KIVIHANTPSYVGSHITGFSSMVKAMVTYLSENT-----KNKNGKVNVLPG-FVGPADMR 181 Query: 190 ELKEMIASFDIEVIVLPDLSSSLTGHLMTGHTPLSRGGVPLDYLREMLTSTYTLALGG-S 248 ELK++ + I++PD S + + + +GG +D LR+ S YT ALG + Sbjct: 182 ELKKIFKCMKVPFIMMPDTSGVVDSPMTGEYKMYPKGGTTIDELRDAGNSDYTFALGSLA 241 Query: 249 MEACAKLLKEELQIPYQVFQGVTGLRETDEFFLFLRQFSQSDGQNKYRWQRQFLLDCMLD 308 EA A L+++ ++P+ V G+ TD F + L + + + +R L+D MLD Sbjct: 242 SEAAAIELEKKCKVPFSVLNTPIGIDATDRFIMELSKITGKKVPYEIEEERGQLVDLMLD 301 Query: 309 TRSVFRGKRIIAALEPDHLIALYQCLAELGIKS----VGSVIPAGSPAVAAMPE------ 358 + F GK++ + +PD +IAL + + LG+ G+ + M E Sbjct: 302 SYQYFHGKKVAISGDPDTVIALTEFVISLGMNPKYVITGTKGEKFEKTINKMLEDNGIEG 361 Query: 359 -AVQT-GDLEDLERMAAGG-ADLWVSNAYGERHALKSGIPFLPLGFPVFNRFGAPANISV 415 V++ DL +L + DL + N++ + A IP + GFPV +R+G V Sbjct: 362 SKVKSFSDLFELHQWIKNEPVDLVIGNSHMKFIARAEDIPLVRFGFPVMDRYGHSYIPKV 421 Query: 416 GYRGTADLLNNIANTM 431 GY+G +LL ++ + + Sbjct: 422 GYKGAMNLLTSMCDAI 437 >MTH1564 [C] COG2710 Nitrogenase molybdenum-iron protein, alpha and beta chains Length = 268 Score = 182 bits (462), Expect = 9e-46, Method: Compositional matrix adjust. Identities = 0/447 (0%), Positives = 0/447 (0%), Gaps = 35/447 (7%) Query: 9 AKPLSVNPFQTSQIIGAVLALQGIYRAVPILHGAQGCAESVKTVLSRHFREPISIQNIAI 68 +I + L ++ + + + Sbjct: 10 IPDSLTAETGDLKIKTYKVVLIARAASIFGVKRIVIYHDDADGEARFIRDILTYMDTPQY 69 Query: 69 HEQNLIFGGEKGIRDMIRLVVEKYNPDVLALIGTSITEVVGEDLLGTAEGYYRQHKMMFK 128 + + + I + + + + + + Sbjct: 70 LRRKVFPIMRELKHVGILPPLRTPHHPTGKPVTGEYRQGLTVKR----VKKGTLVDIGAD 125 Query: 129 DKL-------MCGLYLPDYEGSMESGYAKSVYAVIQKVIQNSGXXXXXXXXXXXXLLPGA 181 + + + + + Sbjct: 126 KLALCREKLTVNRIMSFRVVRLGKEILIEPDEPEDRYWGYEVL-----DTRRNLAESLKT 180 Query: 182 HLTPGDVMELKEMIASFDIEVIVLPDLSSSLTGHLMTGHTP----LSRGGVPLDYLREML 237 + V+ + + + + P Sbjct: 181 -VGADVVVATSRNASPITSILDEVKTRMRGAREAAILFGGPYKGLPEIDADIWVNTLPGQ 239 Query: 238 TSTYTLALGGSMEACAKLLKEELQIPYQVFQGVTGLRETDEFFLFLRQFSQSDGQNKYRW 297 Sbjct: 240 - 298 Query: 298 QRQFLLDCMLDTRSVFRGKRIIAAL-EPDHLIALYQCLAELGIKSV-------GSVIPAG 349 Sbjct: 299 358 Query: 350 SPAVAAMPE----AVQTGDLEDLER-MAAGGADLWVSNAYGERHALKSGIPFLPLGFPVF 404 Sbjct: 359 418 Query: 405 NRFGAPANISVGYRGTADLLNNIANTM 431 Sbjct: 419 445 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.138 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 32,114,567 Number of extensions: 1331151 Number of successful extensions: 3816 Number of sequences better than 1.0e-05: 17 Number of HSP's gapped: 3771 Number of HSP's successfully gapped: 17 Length of query: 439 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 330 Effective length of database: 25,807,025 Effective search space: 8516318250 Effective search space used: 8516318250 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 117 (49.7 bits)