BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0584.0004.p (453 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value all1438 [C] COG2710 Nitrogenase molybdenum-iron protein, alpha a... 356 3e-98 mlr5908 [C] COG2710 Nitrogenase molybdenum-iron protein, alpha a... 322 1e-87 SMa0830 [C] COG2710 Nitrogenase molybdenum-iron protein, alpha a... 316 5e-86 CAC0258 [C] COG2710 Nitrogenase molybdenum-iron protein, alpha a... 228 2e-59 MTH1565 [C] COG2710 Nitrogenase molybdenum-iron protein, alpha a... 221 2e-57 >all1438 [C] COG2710 Nitrogenase molybdenum-iron protein, alpha and beta chains Length = 480 Score = 356 bits (914), Expect = 3e-98, Method: Compositional matrix adjust. Identities = 178/453 (39%), Positives = 278/453 (61%), Gaps = 10/453 (2%) Query: 5 VPNLFIEPDCEHNR-----QPGKGCAR-PNPGEVTKGCVFQGSQAVLLPISDAAHLVHGT 58 + L E CEHN+ + K C++ PG GC F G+ L+PI DAAHLVHG Sbjct: 8 INELLNESGCEHNQHKHGEKKNKSCSQQAQPGAAQGGCAFDGAMISLVPIVDAAHLVHGP 67 Query: 59 SGCLQNSWGMLEAAMPEGPLPY-LRFSMGLTETDIILGGQKKLLEAIQYIIEYYHPPCIF 117 C NSWG ++ GP Y + F+ ++E D+I GG+KKL +AI I + Y+P +F Sbjct: 68 IACAGNSWGS-RGSLSSGPQLYKMGFTTDMSENDVIFGGEKKLYKAILEIHKRYNPSAVF 126 Query: 118 VYATCITILTAEDLNAVCSKAESLWKLPVIPLHNSGFTGSGNMGSRQAGEALFEKVIGTC 177 VYATC+T L +D++AVC A PVIP+ GF GS N+G+R GE+L + V+GT Sbjct: 127 VYATCVTALIGDDIDAVCKTAAEKIGTPVIPVIAPGFIGSKNLGNRFGGESLLDYVVGTA 186 Query: 178 EPDPRKLTPFDINLIGDYHFSKAGHEIEAMLSKIGIRVISRIAGECTFDELCSAHQAKLN 237 EP+ TP+DINLIG+Y+ + + +L K+GIRV+S+I G+ F+E+ AH+AKLN Sbjct: 187 EPE--YTTPYDINLIGEYNIAGEMWGVLPLLEKLGIRVLSKITGDARFEEIRYAHRAKLN 244 Query: 238 LLVCSRSMVTLARKMRDSFGIPYFEGSFCGSREIRFALRQLSFHFQNEGLDKQLNRYMRR 297 +++CSR+++ +ARKM +++GIPY E SF G ++ LR ++ + L + + + Sbjct: 245 VMICSRALLNMARKMEENYGIPYIEESFYGIDDMNRCLRNIAAKLGDPDLQARTEKLIAE 304 Query: 298 EEERLHKDILRRYKPLKGKKVVLFTDGHESWIYISLLYELGIKIAAIGTNQNAQEDLSRI 357 E L + LKGK+VVL+T G +SW IS +LGI++ A T ++ +ED ++I Sbjct: 305 ETAALDLALAPYRARLKGKRVVLYTGGVKSWSIISAAKDLGIEVVATSTRKSTEEDKAKI 364 Query: 358 KERIHDDTLLIIQSDETRILQLYRERKGDLMIVSGRNSFVPLKEKIPFINIGEEQHGGYA 417 K + D +++ + + +LQL ++ + D++I GRN + LK +IPF++I +E+H YA Sbjct: 365 KRLLGADGIMLEKGNAKELLQLVKDTQADMLIAGGRNQYTALKARIPFLDINQERHHPYA 424 Query: 418 GYDGVRRFAQDVMDTLAQPVWTMIRRHSPWEVD 450 GY G+ A+++ + L P+W IR+ +PW+ D Sbjct: 425 GYVGMIEMARELYEALYSPIWEQIRKPAPWDED 457 >mlr5908 [C] COG2710 Nitrogenase molybdenum-iron protein, alpha and beta chains Length = 496 Score = 322 bits (824), Expect = 1e-87, Method: Compositional matrix adjust. Identities = 167/455 (36%), Positives = 264/455 (58%), Gaps = 13/455 (2%) Query: 5 VPNLFIEPDCEHNRQPG-----KGCARP-NPGEVTKGCVFQGSQAVLLPISDAAHLVHGT 58 + ++F EP C+ NR +GC++P PG GC F G++ VL PI+D AHLVH Sbjct: 8 IQDVFDEPACDTNRGKDAKARKEGCSKPLTPGASAGGCAFDGAKIVLQPITDVAHLVHAP 67 Query: 59 SGCLQNSWGMLEAAMPEGPLPYLRFSMGLTETDIILG-GQKKLLEAIQYIIEYYHPPCIF 117 C NSW AA L F+ LTE DI++G G++KL +AI+ I E Y PP +F Sbjct: 68 LACEGNSWDNRGAASSGPTLWRTSFTTDLTELDIVMGQGERKLFKAIREIKETYAPPAVF 127 Query: 118 VYATCITILTAEDLNAVCSKAESLWKLPVIPLHNSGFTGSGNMGSRQAGEALFEKVIGTC 177 VY+TC+T L +D+ AVC +A + L V+P++ G GS N+G++ A EAL + VIGT Sbjct: 128 VYSTCVTALIGDDIEAVCKRAAEKFGLAVVPINAPGLAGSKNLGNKLAAEALLDHVIGTV 187 Query: 178 EPDPRKLTPFDINLIGDYHFSKAGHEIEAMLSKIGIRVISRIAGECTFDELCSAHQAKLN 237 EPD P+DIN++G+++ S ++ +L ++GIRV + I G+ + ++ SAH+A+ Sbjct: 188 EPD--DAGPYDINILGEFNLSGEFWLVKPLLDRLGIRVRACIPGDARYIDVASAHRARAA 245 Query: 238 LLVCSRSMVTLARKMRDSFGIPYFEGSFCGSREIRFALRQLSFHFQNEGLDKQL---NRY 294 ++VCS +++ LARKM + + IPYFEGSF G + ALR ++ +G D ++ Sbjct: 246 MMVCSSALINLARKMEERWDIPYFEGSFYGITDTSEALRNIAELLVRKGADAEILDRTET 305 Query: 295 MRREEERLHKDILRRYKP-LKGKKVVLFTDGHESWIYISLLYELGIKIAAIGTNQNAQED 353 + E+E + L Y+ L+GK+V+L T G +SW + L E+G++I ++ ED Sbjct: 306 LIAEQEAIAWKKLEAYRRRLQGKRVLLNTGGVKSWSVVHALMEIGMEIVGTSVKKSTVED 365 Query: 354 LSRIKERIHDDTLLIIQSDETRILQLYRERKGDLMIVSGRNSFVPLKEKIPFINIGEEQH 413 RIK + D+ + Q + + + K D+M+ GR FV LK K P+++I +E+ Sbjct: 366 KERIKRILKDENHMFEQMAARDLYAMLSKHKADIMLSGGRTQFVALKAKTPWLDINQERQ 425 Query: 414 GGYAGYDGVRRFAQDVMDTLAQPVWTMIRRHSPWE 448 YAGYDG+ + + + P+W +R +PW+ Sbjct: 426 HPYAGYDGMVELVRQIDLAIHNPIWGQVREPAPWD 460 >SMa0830 [C] COG2710 Nitrogenase molybdenum-iron protein, alpha and beta chains Length = 476 Score = 316 bits (809), Expect = 5e-86, Method: Compositional matrix adjust. Identities = 163/453 (35%), Positives = 262/453 (57%), Gaps = 13/453 (2%) Query: 8 LFIEPDCEHNRQPG-----KGCARPN-PGEVTKGCVFQGSQAVLLPISDAAHLVHGTSGC 61 F EP CE NR KGC++P PG GC F G++ L PI++ AHL+H C Sbjct: 11 FFNEPACERNRSKDFKVRKKGCSQPPMPGAAAGGCAFDGAKVALQPITNVAHLIHAPLAC 70 Query: 62 LQNSWGMLEAAMPEGPLPYLRFSMGLTETDIILG-GQKKLLEAIQYIIEYYHPPCIFVYA 120 NSW A L F+ +TE D+++G ++KL +AI+ I E Y P +FVYA Sbjct: 71 EGNSWDNRGTASSSHMLWRTSFTTDVTEFDVVMGHSERKLFKAIREINEAYAPAAVFVYA 130 Query: 121 TCITILTAEDLNAVCSKAESLWKLPVIPLHNSGFTGSGNMGSRQAGEALFEKVIGTCEPD 180 TC+T L +D++AVC +A + LPV+P++ GF GS N+G++ AGEAL + VIGT EPD Sbjct: 131 TCVTALIGDDIDAVCRRAAEKFGLPVVPVNAPGFVGSKNLGNKLAGEALLDHVIGTVEPD 190 Query: 181 PRKLTPFDINLIGDYHFSKAGHEIEAMLSKIGIRVISRIAGECTFDELCSAHQAKLNLLV 240 + + DIN++G+++ S ++ +L K+G+RV + I G+ + ++ +AH+A+ ++V Sbjct: 191 DARSS--DINILGEFNLSGEFWQVRPLLDKLGVRVRACIPGDSRYLDIATAHRARAAMMV 248 Query: 241 CSRSMVTLARKMRDSFGIPYFEGSFCGSREIRFALRQLSFHFQNEGLDKQL---NRYMRR 297 CS +++ LARKM + + IP+FEGSF G + ALRQ++ +G L + Sbjct: 249 CSTALINLARKMLERWDIPFFEGSFYGITDTSEALRQIAGLLVKQGAGPDLISRTEALIV 308 Query: 298 EEERLHKDILRRYKP-LKGKKVVLFTDGHESWIYISLLYELGIKIAAIGTNQNAQEDLSR 356 EEE L Y+P L+GK+V+L T G +SW L E+G++I ++ D R Sbjct: 309 EEEARAWRRLEVYRPRLQGKRVLLNTGGVKSWSVAHALMEIGLEIVGTSIKKSTDNDKER 368 Query: 357 IKERIHDDTLLIIQSDETRILQLYRERKGDLMIVSGRNSFVPLKEKIPFINIGEEQHGGY 416 +K+ + +D+ + + + + K D+M+ GR F+ LK K+P+++I +E+ Y Sbjct: 369 LKQMLTNDSRMSGATTPRELYSALSDHKADIMLSGGRTQFIALKAKMPWLDINQERQHSY 428 Query: 417 AGYDGVRRFAQDVMDTLAQPVWTMIRRHSPWEV 449 AGY GV A+ + ++ P+W +R +PWE+ Sbjct: 429 AGYHGVVELARQIDLSIHNPIWAQVREAAPWEM 461 >CAC0258 [C] COG2710 Nitrogenase molybdenum-iron protein, alpha and beta chains Length = 466 Score = 228 bits (581), Expect = 2e-59, Method: Compositional matrix adjust. Identities = 134/433 (30%), Positives = 234/433 (54%), Gaps = 11/433 (2%) Query: 16 HNRQPGKGCARPNPGEVT-----KGCVFQGSQAVLLPISDAAHLVHGTSGCLQNSWGMLE 70 +N Q G R + V+ + CV+ G++ VL PI+DA HL+HG GC +W + Sbjct: 38 YNSQNKSGTLRCDENSVSGAVSQRACVYCGARVVLNPITDAFHLIHGPIGCASYTWDLRG 97 Query: 71 AAMPEGPLPYLRFSMGLTETDIILGGQKKLLEAIQYIIEYYHPPCIFVYATCITILTAED 130 + E L FS L ETD+I GG+KKL AI I +HP IF+YATCI + +D Sbjct: 98 SLSSESELFRNSFSTDLRETDVIFGGEKKLRAAIDEIFIKFHPKLIFIYATCIVGVIGDD 157 Query: 131 LNAVCSKAESLW-KLPVIPLHNSGFTGSGNMGSRQAGEALFEKVIGTCEPDPRKLTPFDI 189 ++AVC +AE + + VI + + GF+G+ MG R A A+ K+IG + D + + Sbjct: 158 IDAVCREAEIKYPMMRVIAVKSPGFSGNKAMGYRAACNAIL-KLIGDRKSDKK---VKGV 213 Query: 190 NLIGDYHFSKAGHEIEAMLSKIGIRVISRIAGECTFDELCSAHQAKLNLLVCSRSMVTLA 249 N +GD++ + I++ L KIG+ V+++I G+ ++EL A +A LN++ C+ SM LA Sbjct: 214 NYLGDFNLAGEAWVIQSYLKKIGVPVVAKITGDGKYEELLKAPEASLNIVQCAGSMEYLA 273 Query: 250 RKMRDSFGIPYFEGSFCGSREIRFALRQLSFHFQNEGLDKQLNRYMRREEERLHKDILRR 309 +KM++ +GIPY SF G + +L Q++ E + ++ ++++ EE+++ I Sbjct: 274 KKMQELYGIPYIRISFLGVEDTENSLLQIAHILGGEDIVEKTEKFIQEEEDKVKDKIDFY 333 Query: 310 YKPLKGKKVVLFT-DGHESWIYISLLYELGIKIAAIGTNQNAQEDLSRIKERIHDDTLLI 368 K L+GKK ++ G+++ I LGI+ +GT D IKE + T+++ Sbjct: 334 RKRLEGKKAAIYVGGGYKAISLIKQFKSLGIETVMVGTQTGKPRDYEIIKEITNRGTVIL 393 Query: 369 IQSDETRILQLYRERKGDLMIVSGRNSFVPLKEKIPFINIGEEQHGGYAGYDGVRRFAQD 428 ++ + + + E+ D+++ + + K + F + E+ +G+ G FA++ Sbjct: 394 DDANPSELERFMLEKGADILVGGVKERPLAYKLGVAFCDHNHERKHILSGFIGALNFAEE 453 Query: 429 VMDTLAQPVWTMI 441 + T+ PVW + Sbjct: 454 IDLTVNSPVWKYV 466 >MTH1565 [C] COG2710 Nitrogenase molybdenum-iron protein, alpha and beta chains Length = 268 Score = 221 bits (563), Expect = 2e-57, Method: Compositional matrix adjust. Identities = 0/431 (0%), Positives = 0/431 (0%), Gaps = 13/431 (3%) Query: 16 HNRQPGKG---CARPN-PGEVT-KGCVFQGSQAVLLPISDAAHLVHGTSGCLQNSWGMLE 70 ++ + G L+ Sbjct: 39 GVKRIVIYHDDADGEARFIRDILTYMDTPQYLRRKVFPIMRELKHVGILPPLRTPHHPTG 98 Query: 71 AAMPEGPLPYLRFSMGLTETDIILGGQKKLLEAIQYIIEYYHPPCIFVYATCITILTAED 130 + L T + +G K L + + + I I E Sbjct: 99 KPVTGEYRQGLTVKRVKKGTLVDIGADKLALCREKLTVNRIMSFRVVRLGKEILIEPDEP 158 Query: 131 LNAVCSKAESLWKLPVIPLHNSGFTG-SGNMGSRQAGEALFEKVIGTCEPDPRKLTPFDI 189 + + + + + + T R+ I Sbjct: 159 EDRYWGYEVLDTRRNLAESLKTVGADVVVATSRNASPITSILDEVKTRMRGAREAA---I 215 Query: 190 NLIGDYHFSKAGHEIEAMLSKIGIRVISRIAGECTFDELCSAHQAKLNLLVCSRSMVTLA 249 Sbjct: 216 275 Query: 250 RKMRDSFGIPYFEGSFCGSREIRFALRQLSFHFQNEGLDKQLNRYMRREEERLHKDILRR 309 Sbjct: 276 ---- 331 Query: 310 YKPLKGKKVVLFTDGHESWIYISLLYELGIKIAAIGTNQNAQEDLSRIKERIHDDTLLII 369 Sbjct: 332 391 Query: 370 QSDETRILQLYRERKGDLMIVSGRNSFVPLKEKIPFINIGEEQHGGYAGYDGVRRFAQDV 429 Sbjct: 392 451 Query: 430 MDTLAQPVWTM 440 Sbjct: 452 462 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.322 0.140 0.428 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 35,460,755 Number of extensions: 1550939 Number of successful extensions: 2336 Number of sequences better than 1.0e-05: 19 Number of HSP's gapped: 2287 Number of HSP's successfully gapped: 19 Length of query: 453 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 343 Effective length of database: 25,691,691 Effective search space: 8812250013 Effective search space used: 8812250013 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 117 (49.7 bits)