BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0584.0001.p (501 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value sll1167 [V] COG1680 Beta-lactamase class C and other penicillin ... 169 1e-41 MA1345 [V] COG1680 Beta-lactamase class C and other penicillin b... 139 1e-32 BH0715 [V] COG1680 Beta-lactamase class C and other penicillin b... 133 8e-31 SA2230 [V] COG1680 Beta-lactamase class C and other penicillin b... 114 3e-25 PA1797 [V] COG1680 Beta-lactamase class C and other penicillin b... 111 2e-24 >sll1167 [V] COG1680 Beta-lactamase class C and other penicillin binding proteins Length = 414 Score = 169 bits (427), Expect = 1e-41, Method: Compositional matrix adjust. Identities = 138/420 (32%), Positives = 202/420 (48%), Gaps = 30/420 (7%) Query: 25 ILWGFTPHMNAESAQPSTAGSIEEFKQLLDRKVPQWQETYGVPGVAVGVIHNGKVAYTLN 84 +LWG + + +P S+ ++ L+DR E + A+ +IHNGKV Sbjct: 4 VLWGESIWFSHPPLRPGDRASLNQY--LIDRL--NQGEGKELGSAALALIHNGKVDTIHT 59 Query: 85 YGVADTKSHQPVD-KDTLFQAGSISKSLTAWGILHLVDQGRLSLDDPAAKYLTRWKLPDS 143 GV + S PVD ++TL+Q S+SK +TAWG++ LV+ G+++LDDP +L RW+ P Sbjct: 60 VGVENVDSGSPVDPENTLYQMASVSKLVTAWGVMKLVEMGQINLDDPVLSHLRRWQFPAD 119 Query: 144 KFDPSQVTIRRLLSHTAGLPAHRGYLGTAPGKKLPALEESLS----GAGWSNDPVRVAAA 199 +VTI +LLSHT G H GY G PG+ L LE+SL+ A V Sbjct: 120 SPYRGEVTIAQLLSHTGGQNDHLGYGGFLPGQPLQTLEQSLTLTKDVAFGEPRGVEAVYP 179 Query: 200 PGTQEVYSGAGYTLLQLVIEEVTGQPFEQYMDEQVLKPLGMKHSTFAATT-----GSPFM 254 PG + YSG GYT+LQL++EEVTGQ F ++M EQ+L PLGM + F T + + Sbjct: 180 PGEEFSYSGGGYTVLQLLVEEVTGQSFAEFMQEQILHPLGMTGANFDWPTIVDQGRANNL 239 Query: 255 STGYGYFGQALPDYQFTEKAAAGLSTTSADLMTLIIDSMNGRNGQLPVNQVAAKPLIQEM 314 +T + Q P FT AA L + AD++ L N V +Q M Sbjct: 240 ATSFTEDLQPSPPRHFTATGAASLYASLADMVKFA-------QAHLQPNSVLKADTLQRM 292 Query: 315 QTPVL-TDS----GLGIFTRTLPDGQVMLYHPGDNRGFHSFYGFVPESGDGLAVLTNSEN 369 T TD G+ ++ T G V+ + G+ + PE+G+G+ +L + Sbjct: 293 ATAQPGTDGTWGLGMMLYQTTTSGGAVIGHDGGNMPALNHTLRVNPETGNGIVLLLSGNR 352 Query: 370 GIDLRQDIYNAWVQSLTGSVPDGA-DALAAGRQTNAYITQGLAA-ALGLYLVIFAVRLRK 427 L + + WV TG + A + R A I G A A+GL I LR+ Sbjct: 353 --SLASHLGDDWVYWETGKLTTAARERFWQSRLVPALIVIGAGAIAIGLLTFITPGLLRQ 410 >MA1345 [V] COG1680 Beta-lactamase class C and other penicillin binding proteins Length = 252 Score = 139 bits (350), Expect = 1e-32, Method: Compositional matrix adjust. Identities = 0/384 (0%), Positives = 0/384 (0%), Gaps = 29/384 (7%) Query: 2 KRKEKTIFFRGLGLSMMIAT-SLTILWGFTPHMNAESAQPSTAGSIEEFKQLLDRKVPQW 60 + + S +L P S A E Q+ Sbjct: 24 LARVPLENVEYVADLGCGPGNSTALLQQRWPAARITGIDSSPAMIAEAR---SALPDCQF 80 Query: 61 QETYGVPGVAVGVIHNGKVAYTLNYGVADTKSHQPV-DKDTLFQAGSISKSLTAWGILHL 119 E V + +L + + + ++ H+ Sbjct: 81 VEADIRNWQPVQALDLIFANASLQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHV 140 Query: 120 VDQGRLSLDDPAAKYLTRWKLPDSKFDPSQVTIRRLLSHTAGLP--AHRGYLGTAPGKKL 177 + + + + + +D ++ Sbjct: 141 LMREVAWEQNYPDRGREPLAGVHAYYDI--LSEAGCEVDIWRTTYYHQMPSHQAIIDWVT 198 Query: 178 PALEESLSGAGWSNDPVRVAAAPGTQEVYSGAGYTLLQLVIEEVTGQPFEQYMDEQVLKP 237 Sbjct: 199 ATGLRPWLQDL----TESEQQ- 253 Query: 238 LGMKHSTFAATTGSPF---MSTGYGYFGQALPDYQFTEKA---AAGLSTTSADLMTLIID 291 Sbjct: 254 313 Query: 292 SM-NGRNGQLPVNQVAAKPLIQEMQTPVLTDS-GL-GI---FTRTLPDGQVMLYHPGDNR 345 Sbjct: 314 --- 370 Query: 346 GFHSFYGFVPESGDGLAVLTNSEN 369 Sbjct: 371 394 >BH0715 [V] COG1680 Beta-lactamase class C and other penicillin binding proteins Length = 252 Score = 133 bits (334), Expect = 8e-31, Method: Compositional matrix adjust. Identities = 0/362 (0%), Positives = 0/362 (0%), Gaps = 36/362 (9%) Query: 48 EFKQLLDRKVPQWQETYGVPGVAVGVIHNGKVAYTLNYGVADTKSHQPVDKDTLFQAGSI 107 + + +A+ D + Sbjct: 29 LENVEYVADLGCGPGNSTALLQQRWPAARITGIDSSPAMIAE-ARSALPDCQFVEADIRN 87 Query: 108 SKSLTAWGILHLVDQGRLSLDDPAAKYLTRWKLPDSKFDPSQVTIRRLLSHTAGLPAHRG 167 + + A ++ + D L + + L T L Sbjct: 88 WQPVQALDLIFANASLQWLPDHYELFPHLVSLLNP-QGVLAVQMPDNWLEPTHVLMR--- 143 Query: 168 YLGTAPGKKLPALEESLSGAGWSNDPVRVAAAPGTQEVYSGAGYTLLQLVIEEVTGQPFE 227 + E + + Q +I+ VT Sbjct: 144 EVAWEQNYPDRGREPLAGVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLR 203 Query: 228 QYMDEQVLKPLGMKHSTFAATTGSPFMSTGYGYFGQALPDYQFTEKAAA---------GL 278 ++ + + + Y F Sbjct: 204 PWLQDLTESEQQLFLKRYHQMLEE-----QYPLQENGQILLAFPRLFIV 258 Query: 279 STTSADLMTLIIDSM--NGRNGQLPVNQVA--AKPLIQEMQTPVLTDSGLGIFTRTLPDG 334 Sbjct: 259 --- 315 Query: 335 QVMLYHPGDNRGFHSFYGFVPESGDGLAVLTNSENGID------LRQDIYNAWVQSLTGS 388 Sbjct: 316 ---- 371 Query: 389 VP 390 Sbjct: 372 373 >SA2230 [V] COG1680 Beta-lactamase class C and other penicillin binding proteins Length = 252 Score = 114 bits (286), Expect = 3e-25, Method: Compositional matrix adjust. Identities = 0/525 (0%), Positives = 0/525 (0%), Gaps = 60/525 (11%) Query: 6 KTIFFRGLGLSMMIATSLTILWGFTPHMNAESAQPSTAGSIEEFKQLLDRKVPQWQETYG 65 + ++ +L + E+ + + Sbjct: 4 WNPSLYLHFSAERSRPAVELLAR----VPLENVEYVADLGCGPGNSTALLQQRWPA---- 55 Query: 66 VPGVAVGVIHNGKVAYTLNYGVADTKSHQPVDKDTLFQAGSISKSLTAWGILHLVDQGRL 125 + + + I + + + + Sbjct: 56 ARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPVQALDLIFANASLQWLPDHYELFPH 115 Query: 126 SLDDPAAKYLTRWKLPDSKFDPSQVTIRRLLSHTAGLPAHRGYLGTAPGK--KLPALEES 183 + + + ++ + + R ++ P + + Sbjct: 116 LVSLLNPQGVLAVQM-PDNWLEPTHVLMREVAWEQNYPDRGREPLAGVHAYYDILSEAGC 174 Query: 184 LSGAGWSNDPVRVAAAPGTQEVYSGAGYTLLQLVIEEVTGQPFEQYMDEQVLKPLGMKHS 243 + + + E + + M Sbjct: 175 EVDI-------WRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFLKRYHQMLEE 227 Query: 244 TFAATTG-SPFMSTGYGYFGQ----ALPDYQFTEKAAAGLSTTSADLMTLIIDSMNGRNG 298 + ++ + Sbjct: 228 QYPLQENGQILLAFPRLFIVARRME 287 Query: 299 QLPVNQVAAKPLIQEMQTPVLTDSGLGIFTRTLPDGQVMLYHPGDNRGFHSFYGFVPESG 358 Sbjct: 288 --- 344 Query: 359 DGLAVLTNSENGIDLRQDIYNAWVQSLTGSVPDGADALAAGRQTNAY-----ITQGLAAA 413 Sbjct: 345 ------ 398 Query: 414 LGLYLVIFA-----------VRLRKRRRVFITKHSQQPFIRLGIRVVLLTATGTLLFLVS 462 Sbjct: 399 --- 455 Query: 463 YSWSI------LGLQSGLKNIVLVVFAWHLILLAAGFFPKTKIRK 501 Sbjct: 456 --- 497 >PA1797 [V] COG1680 Beta-lactamase class C and other penicillin binding proteins Length = 252 Score = 111 bits (278), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 0/279 (0%), Positives = 0/279 (0%), Gaps = 21/279 (7%) Query: 44 GSIEEFKQLLDRKVPQWQETYGVPGVAVGVIHNGKVAYTLNYGVADTKSHQPVDKDTLFQ 103 + + Q + + + Sbjct: 34 YVADLGCGPGNSTALLQQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPVQA 93 Query: 104 AGSISKSLTAWGILHLVDQGRLSLDDPAAKYLTRWKLPDSKFDPSQ------VTIRRLLS 157 I + + + + + + + ++ + V + Sbjct: 94 LDLIFANASLQWLPDHYELFPHLVSLLNPQGVLAVQM-PDNWLEPTHVLMREVAWEQNYP 152 Query: 158 HTAGLPAHR--GYLGTAPGKKLPALEESLSGAGWSNDPVRVAAAPGTQEVYSGAGYTLLQ 215 P Y + ++ + + P + L Sbjct: 153 DRGREPLAGVHAYYDILS-EAGCEVDIWRTTY-YHQMP------SHQAIIDWVTATGLRP 204 Query: 216 LVIEEVTGQPFEQYMDEQVLKPLGMKHSTFAATTGSPFMSTGYGYFGQALPDYQFTEKAA 275 + + + + Sbjct: 205 WLQDLTESEQQLFLKRYHQMLEEQYPLQEN-- 262 Query: 276 AGLSTTSADLMTLIIDSMNGRNGQLPVNQVAAKPLIQEM 314 Sbjct: 263 -- 299 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.136 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 38,976,069 Number of extensions: 1689610 Number of successful extensions: 4224 Number of sequences better than 1.0e-05: 59 Number of HSP's gapped: 4126 Number of HSP's successfully gapped: 59 Length of query: 501 Length of database: 38,378,431 Length adjustment: 111 Effective length of query: 390 Effective length of database: 25,576,357 Effective search space: 9974779230 Effective search space used: 9974779230 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 118 (50.1 bits)