BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0583.0002.p (285 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value lin0128 [M] COG5632 N-acetylmuramoyl-L-alanine amidase 127 2e-29 >lin0128 [M] COG5632 N-acetylmuramoyl-L-alanine amidase Length = 289 Score = 127 bits (319), Expect = 2e-29, Method: Compositional matrix adjust. Identities = 62/142 (43%), Positives = 87/142 (61%), Gaps = 9/142 (6%) Query: 2 LTLEQVKAKSAKRLD--GLQPVVKAAATALIERCYAREVNIVITQGLRTIAEQDALYAQG 59 LT + K+ ++L+ G+ + +I++ + + + QG R+ AEQ+ALYAQG Sbjct: 3 LTEAWLIEKANRKLNAGGMYKITSDKTRNVIKKMAKEGIYLCVAQGYRSTAEQNALYAQG 62 Query: 60 RTKPGQIVTNAKGGHSYHNYGLAIDFALLLPDGRNVSWDMNRDGDGDKVADWNEVVQEAK 119 RTKPG IVTNAKGG S HNYG+A+D L DG++V W+ + W +VV K Sbjct: 63 RTKPGAIVTNAKGGQSNHNYGVAVDLCLYTNDGKDVIWE-------STTSRWKKVVAAMK 115 Query: 120 ALGFEWGGDWTSIKDYPHFQMS 141 A GF+WGGDW S KDYPHF++ Sbjct: 116 AEGFKWGGDWKSFKDYPHFELC 137 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.315 0.131 0.377 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 20,437,991 Number of extensions: 852340 Number of successful extensions: 3039 Number of sequences better than 1.0e-05: 1 Number of HSP's gapped: 3039 Number of HSP's successfully gapped: 1 Length of query: 285 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 180 Effective length of database: 26,268,361 Effective search space: 4728304980 Effective search space used: 4728304980 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 115 (48.9 bits)