BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0580.0048.p (319 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH1925 [G] COG1175 ABC-type sugar transport systems, permease co... 263 2e-70 BH0902 [G] COG1175 ABC-type sugar transport systems, permease co... 134 1e-31 alr2532 [G] COG1175 ABC-type sugar transport systems, permease c... 130 3e-30 TM0811 [G] COG1175 ABC-type sugar transport systems, permease co... 129 7e-30 BS_yurN [G] COG1175 ABC-type sugar transport systems, permease c... 122 1e-27 >BH1925 [G] COG1175 ABC-type sugar transport systems, permease components Length = 305 Score = 263 bits (673), Expect = 2e-70, Method: Compositional matrix adjust. Identities = 135/304 (44%), Positives = 195/304 (64%), Gaps = 9/304 (2%) Query: 10 HAESKRPFLTEQRRSRITAYTXXXXXXXXXXXXGLYPIFFTIYLSFFKWDALGPMKFXXX 69 H+ +R +LTE+RR+ ++AY GL+P+ F+ YLSFF+WD L PM + Sbjct: 3 HSAKER-WLTEERRTALSAYAFISPFFILFAVFGLFPMIFSFYLSFFRWDGLNPMSYVG- 60 Query: 70 XMKNYDLVTSDPTFWISFSNTLIMGLMGTVPQIIXXXXXXXXXHSGMTKFKKTFRILYFM 129 + N+ L+ +DPTF+ + NT I+G++GT+PQII +S + +F+ TFR L F+ Sbjct: 61 -LANFQLIFNDPTFFSAIKNTFIIGILGTLPQIIVGILLAFALNSALIRFQHTFRTLVFL 119 Query: 130 PNITSIVAVTLVFSTLFGNN--GMINWMLNGLGLDSVAFNSGWWGVKIAISTMVMWRWTG 187 P ITSIVAV ++F +F N G+ N++L+ +D V ++ +W VKIAI+TMV WRW G Sbjct: 120 PYITSIVAVAIIFGLVFNNQPFGLANYVLSLFNIDPVRWSQEYWPVKIAIATMVFWRWVG 179 Query: 188 YNAIIFLSGLQSIPTDLYEAARIDGANRRQQFTFITMPLLRPFIVFVTLLSTIGALQLFT 247 YN IIFL+G+QSIP +LYEAA+IDGA QQ IT+P+L+PFI+FV +TIG+LQLFT Sbjct: 180 YNTIIFLAGMQSIPKELYEAAKIDGATIGQQIRLITVPMLKPFILFVVFTATIGSLQLFT 239 Query: 248 EPYVFLGQSGTGSTRQEGITMVTYLYSEAFRNXXXXXXXXXXXXXXXXXIIFSLLNMLAS 307 EP +FLG+ R+EGITMV YL+ +AF I + +N+L + Sbjct: 240 EPLIFLGR----GLREEGITMVAYLWRDAFVLNAFGTASAAAIVLFFIIITLTAINLLIT 295 Query: 308 SRMG 311 +R+G Sbjct: 296 NRIG 299 >BH0902 [G] COG1175 ABC-type sugar transport systems, permease components Length = 316 Score = 134 bits (338), Expect = 1e-31, Method: Compositional matrix adjust. Identities = 83/279 (29%), Positives = 132/279 (47%), Gaps = 9/279 (3%) Query: 3 EPVLASPHAESKRPFLTEQRRSRITAYTXXXXXXXXXXXXGLYPIFFTIYLSFFKWDALG 62 E + H T ++ Y YP I +SF + Sbjct: 9 EQEVVQVHTRKSSRLFTFFNSQKVVPYVLISPFILSFIVLSFYPTVQAIVMSF---QRVL 65 Query: 63 PMKFXXXXMKNYDLVTSDPTFWISFSNTLIMGLMGTVPQIIXXXXXXXXXHSGMTKFKKT 122 P + + NY + +PTF+ + NT ++ V + +S + KF+ Sbjct: 66 PSEVTFVGLWNYSRIL-NPTFFTALQNTTTYMILTVVILVSIPMLFAVLLNSKVVKFRIL 124 Query: 123 FRILYFMPNITSIVAVTLVFSTLFG--NNGMINWMLNGLGLDSVAFNSGWWGVKIAISTM 180 FR F+P +TS++ +VF +F + + N +LN +GL+SV + W + + Sbjct: 125 FRTALFLPALTSVIVAGMVFRLMFSESDTAVANQILNWIGLESVEWRYNAWSGMFLMVVL 184 Query: 181 VMWRWTGYNAIIFLSGLQSIPTDLYEAARIDGANRRQQFTFITMPLLRPFIVFVTLLSTI 240 WRW G N + FL+ LQ++P +LYEAA IDGAN Q+F ++T+P L+P +FVT +S I Sbjct: 185 ASWRWMGINILYFLAALQNVPKELYEAADIDGANVVQKFFYVTLPFLKPVTIFVTTISVI 244 Query: 241 GALQLFTEPYVFLGQSGTGSTRQEGITMVTYLYSEAFRN 279 G ++F E +VF GS G+T+V YLY E + Sbjct: 245 GGFRMFEESFVFW---EAGSPGNIGLTIVGYLYQEGIQQ 280 >alr2532 [G] COG1175 ABC-type sugar transport systems, permease components Length = 359 Score = 130 bits (327), Expect = 3e-30, Method: Compositional matrix adjust. Identities = 13/237 (5%), Positives = 38/237 (16%), Gaps = 11/237 (4%) Query: 46 PIFFTIYLSFFKWDALGPMKFXXXXMKNYDLVTSDPTFWISFSNTLIMGLMGTVPQIIXX 105 + DA G + +T F + + + Sbjct: 40 HGLLETARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAV 99 Query: 106 XXXXXXXHSGMTKFKKTFRILYFMPNITSIVAVTLVFSTLFGNNGMINWMLNGLGLDSVA 165 ++ + + + ++ Sbjct: 100 HNEEQLAALEE-ASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNI-- 156 Query: 166 FNSGWWG----VKIAISTMVMWRWTGYNAIIFLSGLQSIPTDLYEAARIDGANRRQQFTF 221 + K + + + + + + + R Sbjct: 157 --VSHFARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIIL 214 Query: 222 ITMPLLRPFIVFVTLLSTIGALQLFTEPYVFLGQSGTGSTRQEGITMVTYLYSEAFR 278 + L + V G G T V+ + Sbjct: 215 YGVSPLEDRSTGADFGCQPVMSLTSSLIAVREH--KAGEPVGYGGTWVSERDTRLGV 269 >TM0811 [G] COG1175 ABC-type sugar transport systems, permease components Length = 300 Score = 129 bits (323), Expect = 7e-30, Method: Compositional matrix adjust. Identities = 86/261 (32%), Positives = 134/261 (51%), Gaps = 10/261 (3%) Query: 45 YPIFFTIYLSFFKWDALGPMKFXXXXMKNYDLVTSDPTFWISFSNTLIMGLMGTVPQIIX 104 YP+ F I +SF+++D +G + +KNY + DP FW +F N+L+ L+ Q+I Sbjct: 34 YPLVFGIKISFYQYDIVGESLYIG--LKNYVDLLRDPLFWNAFKNSLLYLLVVPPLQLIS 91 Query: 105 XXXXXXXXHSGMTKFKKTFRILYFMPNITSIVAVTLVFSTLFGNNGMINWMLNGLGL--D 162 + + + FR L F+P +T I + + ++ G IN++L L + + Sbjct: 92 ILLAVLLDRA--IRGRNLFRTLIFLPVVTPITIAAITWQWMYREKGFINFLLQSLHIIDE 149 Query: 163 SVAFNSGWWGVKIAISTMVMWRWTGYNAIIFLSGLQSIPTDLYEAARIDGANRRQQFTFI 222 +AF S AI + MW+ GY +I+L+GLQSIP +L EAAR+DGA Q F + Sbjct: 150 PIAFLSDPKIALFAIMFVTMWKGFGYYMVIYLAGLQSIPRELIEAARVDGAKPSQVFFKV 209 Query: 223 TMPLLRPFIVFVTLLSTIGALQLFTEPYVFLGQSGTGSTRQEGITMVTYLYSEAFRNXXX 282 T+PLL+P+I+F + +S+I AL +F E Y +T GI ++Y+ AF Sbjct: 210 TIPLLKPYILFCSTMSSIAALNVFGEIYAMTKGGPVHATETMGI----FIYNRAFEYLQF 265 Query: 283 XXXXXXXXXXXXXXIIFSLLN 303 I FSLLN Sbjct: 266 GYSNAAAVLFSFVVIAFSLLN 286 >BS_yurN [G] COG1175 ABC-type sugar transport systems, permease components Length = 292 Score = 122 bits (305), Expect = 1e-27, Method: Compositional matrix adjust. Identities = 70/238 (29%), Positives = 123/238 (51%), Gaps = 13/238 (5%) Query: 46 PIFFTIYLSFFKWDALGPMKFXXXXMKNYDLVTSDPTFWISFSNTLIMGLMGTVPQIIXX 105 PIF ++LS F+W + P K +KNY + DP F+ + +N ++ ++ V Q+ Sbjct: 26 PIFENVFLSLFQWSSFSPEK-TFIGLKNYVELFHDPVFYQALTNNVLYAVISIVCQVFGG 84 Query: 106 XXXXXXXHSGMT-KFKKTFRILYFMPNITSIVAVTLVFSTLFG-NNGMINWMLNGLGLDS 163 + K+ FR ++F+P + S+ + L+F ++ G++N +L +GLD Sbjct: 85 LILAAVLEDKLVRKWSPFFRTVFFLPVVISMTVIALLFDFIYNPETGLLNQLLQAIGLDQ 144 Query: 164 VAFNSGWWG----VKIAISTMVMWRWTGYNAIIFLSGLQSIPTDLYEAARIDGANRRQQF 219 + W G +++ + W+ GY A++++ +Q IP +LYEAAR+DGA + QQF Sbjct: 145 L--TRAWLGDDSTAMLSVIFVSQWQSVGYIAMLYIVSIQKIPDELYEAARLDGAGKIQQF 202 Query: 220 TFITMPLLRPFIVFVTLLSTIGALQLFTEPYVFLGQSGTGSTRQEGITMVTYLYSEAF 277 IT+P + +++ GA +F EPY+ G G G + + T+LY AF Sbjct: 203 FHITVPQTKEMSFVAVVMTLTGAFTVFNEPYILTG-GGPGKASE---VLSTFLYKSAF 256 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.326 0.139 0.429 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 19,022,068 Number of extensions: 678834 Number of successful extensions: 2082 Number of sequences better than 1.0e-05: 159 Number of HSP's gapped: 1964 Number of HSP's successfully gapped: 159 Length of query: 319 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 213 Effective length of database: 26,153,027 Effective search space: 5570594751 Effective search space used: 5570594751 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 116 (49.3 bits)