BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0580.0040.p (385 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_yocF [T] COG4585 Signal transduction histidine kinase 388 e-108 BS_yvfT [T] COG4585 Signal transduction histidine kinase 200 3e-51 BH0582 [T] COG4585 Signal transduction histidine kinase 152 9e-37 DRA0009 [T] COG4585 Signal transduction histidine kinase 136 5e-32 SA1158 [T] COG4585 Signal transduction histidine kinase 125 8e-29 >BS_yocF [T] COG4585 Signal transduction histidine kinase Length = 370 Score = 388 bits (996), Expect = e-108, Method: Compositional matrix adjust. Identities = 193/369 (52%), Positives = 267/369 (72%), Gaps = 2/369 (0%) Query: 4 MQKWHHIFHKSTGLSPYVWVVFYILPFYFIFRSASVNQNQWVYGIVMVAVFFACYVLSFK 63 M K H F K G++PY+W +F+ILPFYFI++S+S + GI++ +FF+ Y +F Sbjct: 1 MIKNHFTFQKLNGITPYIWTIFFILPFYFIWKSSSTFVI--IVGIILTLLFFSVYRFAFV 58 Query: 64 SKGWVVYFWTTVQILVSITMTLLFGYMYFALFIAFFIGNIQKRAGFFTLYSIHLLTTIAA 123 SKGW +Y W + I +S LF Y+YFA FIA+FIGNI++R F LY +HL++ A Sbjct: 59 SKGWTIYLWGFLLIGISTASITLFSYIYFAFFIAYFIGNIKERVPFHILYYVHLISAAVA 118 Query: 124 INYEMITGNRVFISQLPFVLVSMIAVVLLPATTYNRNNQDKLQGQLEDANKRISELVIME 183 N+ ++ F++Q+PFV++++I+ +LLP + +R +++L+ +LEDAN+RI+ELV +E Sbjct: 119 ANFSLVLKKEFFLTQIPFVVITLISAILLPFSIKSRKERERLEEKLEDANERIAELVKLE 178 Query: 184 ERQRIARDLHDTLGQKLSLIGLKSDLAGKLIHKNPAQAAVEINDVRQTARSALKEVREMV 243 ERQRIARDLHDTLGQKLSLIGLKSDLA KLI+K+P QAA E+ V+QTAR++L EVR++V Sbjct: 179 ERQRIARDLHDTLGQKLSLIGLKSDLARKLIYKDPEQAARELKSVQQTARTSLNEVRKIV 238 Query: 244 TQMRGIRLEDELIRIRQLLEAAEIEFQLEGSPKLVNTSLMTENVLSMCLKEAVTNVVKHS 303 + M+GIRL+DELI I+Q+LEAA+I F E N SL+ EN+LSMCLKEAVTNVVKHS Sbjct: 239 SSMKGIRLKDELINIKQILEAADIMFIYEEEKWPENISLLNENILSMCLKEAVTNVVKHS 298 Query: 304 GATLCQVQIEPSRTDLLIKVKDNGTGIEGSNLYDKGHGLQGMRERLEFVNGSMDVLQDKG 363 A C+V I+ +++I V D+GT N + KGHGL GMRERLEF NGS+ + + G Sbjct: 299 QAKTCRVDIQQLWKEVVITVSDDGTFKGEENSFSKGHGLLGMRERLEFANGSLHIDTENG 358 Query: 364 TTLIIRVPN 372 T L + +PN Sbjct: 359 TKLTMAIPN 367 >BS_yvfT [T] COG4585 Signal transduction histidine kinase Length = 328 Score = 200 bits (509), Expect = 3e-51, Method: Compositional matrix adjust. Identities = 115/332 (34%), Positives = 187/332 (56%), Gaps = 12/332 (3%) Query: 50 MVAVFFACYVLSFKSKGWVVY-FWTTVQILVSITMTLLFG--YMYFALFIAFFIGNIQKR 106 M+ +F A Y F S G + +W VQ+ V + ++ + Y+Y F A F+G +++ Sbjct: 1 MLLLFVAAYRQLFCSVGKASFTYWLIVQMAVILMYSVFYNITYIYLGFFPANFVGYYKEK 60 Query: 107 AGFFTLYSIHLLTTIAAINYEMITGN---RVFISQLPFVLVSMIAVVLLPATTYNRNNQD 163 F + + + Y+ I + R S LPF+++ +I+ P + + Sbjct: 61 TNFNRAFCALIFILLFPCLYQFIANSVSLRELFSVLPFLVIMLIS----PFGIRSMFRRI 116 Query: 164 KLQGQLEDANKRISELVIMEERQRIARDLHDTLGQKLSLIGLKSDLAGKLIHKNPAQAAV 223 +L+ +L AN++I EL EER RIARDLHDTLG LSL+ LKS L +L +P + + Sbjct: 117 ELEAKLAQANEQIKELSKREERVRIARDLHDTLGHTLSLLTLKSQLIQRLAASDPERTKL 176 Query: 224 EINDVRQTARSALKEVREMVTQMRGIRLEDELIRIRQLLEAAEIEFQLEGSPKLVNTSLM 283 E ++ ++RSALK+VRE+V+ MR + + +EL+ I+ +L A I FQ EG+ S + Sbjct: 177 EAKEMETSSRSALKQVRELVSDMRTVTITEELVNIQHILRAGNITFQYEGADDFSVISPV 236 Query: 284 TENVLSMCLKEAVTNVVKHSGATLCQVQIEPSRTDLLIKVKDNGTGIEGSNLYDKGHGLQ 343 T+N++SMC++EAVTN++KHS AT C + I + I ++D+G G ++ G+GL Sbjct: 237 TQNIISMCMREAVTNIIKHSKATHCAITISQFADKMRIVIRDDGKGAPKEKMF--GNGLW 294 Query: 344 GMRERLEFVNGSMDVLQDKGTTLIIRVPNVFK 375 GM ERL + G + V GT + + +P + K Sbjct: 295 GMEERLMLIEGGLTVSDHNGTVVALTIPLIKK 326 >BH0582 [T] COG4585 Signal transduction histidine kinase Length = 374 Score = 152 bits (384), Expect = 9e-37, Method: Compositional matrix adjust. Identities = 96/296 (32%), Positives = 170/296 (57%), Gaps = 26/296 (8%) Query: 85 LLFGYMYFALFIAFFIGNIQKRA----GFFTLYSIHLLTTIAAINYEMITGNRVFISQLP 140 LL+G++ ++ F+G + G L+++H+ T A + + V QLP Sbjct: 86 LLYGFI-----VSDFLGRAHAKRLMWIGVVGLFAMHISTAYALYGHWFTFYSPV---QLP 137 Query: 141 FVLVSMIAVVLLPATTYNRNNQDKLQGQLEDANKRISELVIMEERQRIARDLHDTLGQKL 200 +++ ++ LP R L+ +L+ AN+++ + EER RIARDLHDTLGQ L Sbjct: 138 ILILQLV----LPFMIRIREKSKLLKEELDTANQKLERYIQEEERHRIARDLHDTLGQSL 193 Query: 201 SLIGLKSDLAGKLIHKNPAQAAVEINDVRQTARSALKEVREMVTQMRGIRLEDELIRIRQ 260 ++I +KS LA +L+ K+ QA E+ +V T+R LK+VRE+VT M+ + LE+E+ ++ Sbjct: 194 TMITMKSQLATRLVEKDADQAKKEMTEVMNTSRRVLKQVRELVTTMKFVSLEEEVSHAKE 253 Query: 261 LLEAAEIEFQL--EG-SPKLVNTSLMTENVLSMCLKEAVTNVVKHSGATLCQVQIEPSRT 317 +L I ++ EG +P+L+ + E++L++ L+EAVTN++KHS AT C + E + Sbjct: 254 ILSLQNITLEVIQEGATPQLLKVA---ESMLALSLREAVTNIIKHSRATHCTIVKEYANN 310 Query: 318 DLLIKVKDNGTGIEGSNLYDKGHGLQGMRERLEFVNGS--MDVLQDKGTTLIIRVP 371 + ++V DNG G + G+G+ +RER+ + G+ + L + G ++ + +P Sbjct: 311 EYKLQVIDNGIG--KIDQMQTGNGITSIRERIHLLQGNVCLTSLPNGGMSITMTIP 364 >DRA0009 [T] COG4585 Signal transduction histidine kinase Length = 336 Score = 136 bits (343), Expect = 5e-32, Method: Compositional matrix adjust. Identities = 114/342 (33%), Positives = 170/342 (49%), Gaps = 30/342 (8%) Query: 53 VFFACYVLSFKSKGWVVYFWTTVQILVSITMTLLFGYMYFALFIAFFI--GNI--QKRAG 108 F A YV +F + W V L ++ M L ++Y ++F + G++ +R Sbjct: 9 AFVAVYVFTFGKRD-PDDRWAYVGWLSALLMWALGDWLYGPSALSFLVYGGSLIGWQRRQ 67 Query: 109 FFTLYSIHLLTTIAAINYEMITGNRVFI---SQLPFV-LVSMIAVVLLPATTYNRNNQDK 164 + L AAIN ++ G + + SQ F+ L+ ++A TY + Sbjct: 68 WVALGG-------AAINALLMVGRLLTLPERSQGDFIFLLFILASAYANHATYRGLRARR 120 Query: 165 LQGQLEDANKRISELVIMEERQRIARDLHDTLGQKLSLIGLKSDLAGKLIHKNPAQAAVE 224 Q++ ++ L ER+RIARDLHD LG LS+I LKS+LAGKL K+P +AA E Sbjct: 121 RLAQVQLEKEK---LAADAERERIARDLHDLLGHTLSVIVLKSELAGKLAEKHPQRAAAE 177 Query: 225 INDVRQTARSALKEVREMVTQMRGIRLEDELIRIRQLLEAAEIEFQLEGSPKLVNTSLMT 284 I +V + +R AL EVR V RG L EL R + L+AA I + + + L Sbjct: 178 IREVERISREALSEVRAAVQGYRGSGLAAELARAKVALDAAGIRLVITDTLPELPADL-- 235 Query: 285 ENVLSMCLKEAVTNVVKHSGATLCQVQIEPSRTDLLIKVKDNGTGIEGSNLYDKGHGLQG 344 E+ L+M L+EAVTNVV+HSGA+ ++ + + ++D+G G + +G GL Sbjct: 236 ESTLAMVLREAVTNVVRHSGASELRLTLRGQANGFQLTLQDDGRGGDAP----EGTGLNS 291 Query: 345 MRERLEFVNGSMDVLQDKGTTLIIRVP-----NVFKQPELKE 381 MRERL + G+ + GT L VP K PEL E Sbjct: 292 MRERLRAMGGTFERDGHTGTRLQAFVPLSAGTAGLKAPELLE 333 >SA1158 [T] COG4585 Signal transduction histidine kinase Length = 363 Score = 125 bits (315), Expect = 8e-29, Method: Compositional matrix adjust. Identities = 94/372 (25%), Positives = 190/372 (51%), Gaps = 29/372 (7%) Query: 11 FHKSTGLSPYVWVVFYILPFYFIFRSASVNQNQWVYGIVMVAVFFACYVLSFKSKG---- 66 F K T ++ +++ I P IF + V +W+Y I+ V VF Y++ Sbjct: 3 FLKDTSIAEISSILYLIFPIAGIFFN-EVYGPKWLY-IISVIVFSLSYLILVIVNNRLNT 60 Query: 67 ------WVVYFWTTVQILVSITMTLLFGYMYFALFIAF-FIGNIQKRAGFFTLYSIHLLT 119 +++++ + S+ L + Y A + F F N++K A + ++ + T Sbjct: 61 LMFYILLIIHYFIICYFVFSVHPMLSLFFFYSAFAVPFTFKNNVKKTATNLFILTMIICT 120 Query: 120 TIAAINYEMITGNRVFISQLPFVLVSMIAVVLLPATTYNRNNQDKLQGQLEDANKRISEL 179 I + Y N F++ + + +V I++++L + + + Q ++ + N+ I+ L Sbjct: 121 IITYLLY-----NNYFVAMMVYYVV--ISLIMLD--NFKKMKNREYQKEIAEKNRHINTL 171 Query: 180 VIMEERQRIARDLHDTLGQKLSLIGLKSDLAGKLIHKNPAQAAVEINDVRQTARSALKEV 239 + +ER RI +DLHDTLG + + LKS+LA KLI + + E+ + + +R +L +V Sbjct: 172 IAEQERHRIGQDLHDTLGHVFASLSLKSELAYKLIDTDVEKVKAELLAINKLSRESLNKV 231 Query: 240 REMVTQMRGIRLEDELIRIRQLLEAAEIEFQLEGSPKLVNTSLMTENVLSMCLKEAVTNV 299 RE++ ++ +E+ IR++L+ A+I+F E S +++L M +EA+ NV Sbjct: 232 REIIDDVKLPSFIEEIDSIRKVLKDADIDFTFENKELAQVLSPTKQSMLVMITREAINNV 291 Query: 300 VKHSGATLCQVQIEP-SRTDLLIKVKDNGTGIEGSNLYDKGHGLQGMRERLEFVNGSMDV 358 +KH+ A+ +++ + LL+ ++D+G GI D ++ + +R++ +NG++ V Sbjct: 292 IKHANASKVHGKLKTVNNHKLLLMIEDDGKGI------DSDCEVKSISQRVQHLNGTLAV 345 Query: 359 LQDKGTTLIIRV 370 GT +II + Sbjct: 346 DSTNGTKIIIEI 357 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.324 0.138 0.400 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 26,155,331 Number of extensions: 1054644 Number of successful extensions: 3565 Number of sequences better than 1.0e-05: 65 Number of HSP's gapped: 3478 Number of HSP's successfully gapped: 65 Length of query: 385 Length of database: 38,378,431 Length adjustment: 108 Effective length of query: 277 Effective length of database: 25,922,359 Effective search space: 7180493443 Effective search space used: 7180493443 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 117 (49.7 bits)