BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0580.0038.p (499 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CAC3213 [R] COG2244 Membrane protein involved in the export of O... 108 3e-23 BH3277 [R] COG2244 Membrane protein involved in the export of O-... 102 1e-21 BS_spoVB [R] COG2244 Membrane protein involved in the export of ... 98 2e-20 CAC1016 [R] COG2244 Membrane protein involved in the export of O... 93 1e-18 BH1233 [R] COG2244 Membrane protein involved in the export of O-... 89 1e-17 >CAC3213 [R] COG2244 Membrane protein involved in the export of O-antigen and teichoic acid Length = 432 Score = 108 bits (269), Expect = 3e-23, Method: Compositional matrix adjust. Identities = 16/400 (4%), Positives = 62/400 (15%), Gaps = 19/400 (4%) Query: 22 KLTGLIGRVILTRIVGAEGIGLYQIAYSFYGFVLMFTGGLPTTLAMASAKKPAQS----- 76 + V + ++ G + Sbjct: 21 ADSIETAVKSELVNVAKKVRIDGFRKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAII 80 Query: 77 -WSLLKIISLGVILFGGIVSMAVFWNSPAISRFLGNPGLEYSIRG--LAPSXXXLFAVPL 133 + + + + ++ +I Sbjct: 81 KEKINPAGAPTYVPGEYKLGEDFTYSVEFEVYPEVELQGLEAIEVEKPIV-----EVTDA 135 Query: 134 LGLVRGYLQGHKQIGVIALSEIIEQASRIFFMLLIVWHVLPLGINRAIGSGLYGTFIGA- 192 +Q +E R+ +A L Sbjct: 136 DVDGMLDTLRKQQATWKEKDGAVEAEDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMI 195 Query: 193 LSAFSLLTIYISVNKTSLPYNTSYTVQSLPIFWFIQSSLMISATRLIIPASEFIDAVLIP 252 + + + + ++ + + + + + Sbjct: 196 PGFEDGIKGHKAGEEFTIDVTFPEEYHAENLKGKA--AKFAINLKKVEERELPELTAEFI 253 Query: 253 NRLLAAGYSTSEATSMYGVIYGMAVIVAYAPTLLTGALCHTLSVQIAAEWQQGNMQRFNR 312 R S + + A + + A+ + + Sbjct: 254 KRFGVEDGSVEGLRAEVRKNMERELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDV 313 Query: 313 LSATAFKVCWLWGIATALFLKGYAPELSLFIFNTETAGPAI-KYLA-AIPLLVGFREIST 370 L A + + E + + + + + Sbjct: 314 LRRQAAQRFGGNEKQALELPRELFEEQAKRRVVVGLLLGEVIRTNELKADEER-VKGLIE 372 Query: 371 SILWSQDIRKSPFYGLLTGIICATAAQYFLVGIPGFGYKG 410 + + + K + + Sbjct: 373 EMASAYEDPKEVIEFYSKNKELMDNMRNVALEEQAVEAVL 412 >BH3277 [R] COG2244 Membrane protein involved in the export of O-antigen and teichoic acid Length = 432 Score = 102 bits (254), Expect = 1e-21, Method: Compositional matrix adjust. Identities = 30/421 (7%), Positives = 69/421 (16%), Gaps = 44/421 (10%) Query: 3 GSRXXXRQSAIRAFALFIVKLTGLIGRVILTRIVGAEGIGLYQIAYSFYGFVL-MFTGGL 61 Q R + I + V + ++ G Sbjct: 2 QVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGFRKGKVPMNIVAQRYGAS 61 Query: 62 PTTLAMASAKKPAQSWSL------LKIISLGVILF---GGIVSMAVFWNSPAISRFLGNP 112 S + KI G + + ++ Sbjct: 62 V----RQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGEDFTYSVEFEVYPE-VE 116 Query: 113 GLE--------YSIRGLAPSXXXLFAVPLLGLVRGYLQGHKQIGVIALSEIIEQASRIFF 164 + + + + K V A + + Sbjct: 117 LQGLEAIEVEKPIVEVTDAD---VDGMLDTLRKQQATWKEKDGAVEAEDRVTIDFTGSVD 173 Query: 165 ML---LIVWHVLPLGINRAIGSGLYGTFIGALSAFSLLTIY---------ISVNKTSLPY 212 L + + + I A TI ++ + + Sbjct: 174 GEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFPEEYHAENLKGKAAKF 233 Query: 213 NTSYTVQSLPIFWFIQSSLMISATRLIIPASEFIDAVLIPNRLLAAGYSTSEATSMYGVI 272 + R + + + S + Sbjct: 234 AINLKKVEERELPE---LTAEFIKRFGVEDGSVEGLRAEVRKNMERELK-SAIRNRVKSQ 289 Query: 273 YGMAVIVAYAPTLLTGALCHTLSVQIAAEWQQGNMQRFNRLSATAFKVCWLWGI--ATAL 330 ++ A + + + V Q+ L L Sbjct: 290 AIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPRELFEEQAKRRVVVGL 349 Query: 331 FLKGYAPELSLFIFNTETAGPAIKYLAAIPLLVGFREISTSILWSQDIRKSPFYGLLTGI 390 L L G + +A E + D ++ Sbjct: 350 LLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNKELMDNMRNVALEEQAVE 409 Query: 391 I 391 Sbjct: 410 A 410 >BS_spoVB [R] COG2244 Membrane protein involved in the export of O-antigen and teichoic acid Length = 432 Score = 98.2 bits (243), Expect = 2e-20, Method: Compositional matrix adjust. Identities = 26/409 (6%), Positives = 69/409 (16%), Gaps = 29/409 (7%) Query: 20 IVKLTGLIGRVILTRIVGAEGIGLYQIAY-SFYGFVLMFTGGLPTTL----AMASAKKPA 74 I + V + V G M+ A Sbjct: 19 IAADSIETAVKSELVNVAKKVRIDGFRKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDA 78 Query: 75 QSWSLLKIISLGVILFGGIVSMA-----VFWNSPAISRFLGNPGLEYSIRGLAPSXXXLF 129 + + + + GLE Sbjct: 79 IIKEKINPAGAPTY---VPGEYKLGEDFTYSVEFEVYPEVELQGLEAIEVEKPI------ 129 Query: 130 AVPLLGL---VRGYLQGHKQIGVIALSEIIEQASRIFFMLLIVWHVLPLGINRAIGSGLY 186 + L+ + + + V G + Sbjct: 130 VEVTDADVDGMLDTLRKQQATWKEKDGAVEAEDRVTIDFTGSVDGEEFEGGKASDFVLAM 189 Query: 187 GTFIGALSAFSLLTIYISVNKTSLPYNTSYTVQSLP-----IFWFIQSSLMISATRLIIP 241 G I + ++ + +L R + Sbjct: 190 GQGRMIPG--FEDGIKGHKAGEEFTIDVTFPEEYHAENLKGKAAKFAINLKKVEERELPE 247 Query: 242 ASEFIDAVLIPNRLLAAGYSTSEATSMYGVIYGMAVIVAYAPTLLTGALCHTLSVQIAAE 301 + G +M + + + + + V A Sbjct: 248 LTAEFIKRFGVEDGSVEGLRAEVRKNMERELKSAIRNRVKSQAIEGLVKANDIDVPAALI 307 Query: 302 WQQGNMQRFNRLSATAFKVCWLWGIATALFLKGYAPELSLFIFNTETAGPAIKYLAAIPL 361 + ++ R + LF + + + + E + Sbjct: 308 DSEIDVLRRQAAQRFGGNEKQALELPRELFEEQAKRRVVVGLLLGEVIRTNELKADEERV 367 Query: 362 LVGFREISTSILWSQDIRKSPFYGLLTGIICATAAQYFLVGIPGFGYKG 410 E++++ +++ + A Sbjct: 368 KGLIEEMASAYEDPKEVIEFYSKNKELMDNMRNVALEEQAVEAVLAKAK 416 >CAC1016 [R] COG2244 Membrane protein involved in the export of O-antigen and teichoic acid Length = 432 Score = 93.2 bits (230), Expect = 1e-18, Method: Compositional matrix adjust. Identities = 22/428 (5%), Positives = 76/428 (17%), Gaps = 38/428 (8%) Query: 9 RQSAIRAFALF-----IVKLTGLIGRVILTRIVG-AEGIGLYQIAYSFYGFVLMFTG-GL 61 I + V I ++ V G + Sbjct: 3 VSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGFRKGKVPMNIVAQRYGASV 62 Query: 62 PTTL--------AMASAKKPAQSWSLLKIISLGVILFGGIVSMAVFWNSPAISRFLGNPG 113 + + + K + + V + + + + G Sbjct: 63 RQDVLGDLMSRNFIDAIIKEKINPAGAPTY---VPGEYKLGEDFTYSVEFEVYPEVELQG 119 Query: 114 LEYSIRGLAPSXXXLFAVPLLGLVRGYLQGHKQIGVIALSEIIEQASRIFFMLLIVWHVL 173 LE + + + L+ + + + V Sbjct: 120 LEAIEVEKPIVE--VTD-ADVDGMLDTLRKQQATWKEKDGAVEAEDRVTIDFTGSVDGEE 176 Query: 174 PLGINRAIGSGLYGTFIGA-LSAFSLLTIYISVNKTSL----PYNTSYTVQSLPIFWFIQ 228 G + G + T + ++ + I Sbjct: 177 FEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFPEEYHAENLKGKAAKFAIN 236 Query: 229 SSLMISATRLIIPASEFIDAVLIPNRLLAAGYSTSEATSMYGVIYGMAVIVAYAPTLLTG 288 + + A + + + + + A L Sbjct: 237 LKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMERELKSAIRNRVKSQAIEGLVK 296 Query: 289 ---ALCHTLSVQIAAEWQQGNMQRFNRLSATAFKVCWLWGIATALFLKGYAPELSLFIFN 345 + + + + + L + + + Sbjct: 297 ANDIDVPAALIDSEIDVLRRQAAQRF----GGNEKQALELPRELFEEQAKRRVVVGLLLG 352 Query: 346 TETAGPAIKYL-AAIPLLVGFREISTSILWSQDIRKSPFYGL--LTGIICATAAQYFLVG 402 +K + L+ + +++ + + + + + Sbjct: 353 EVIRTNELKADEERVKGLIEEMASAYEDP--KEVIEFYSKNKELMDNMRNVALEEQAVEA 410 Query: 403 IPGFGYKG 410 + Sbjct: 411 VLAKAKVT 418 >BH1233 [R] COG2244 Membrane protein involved in the export of O-antigen and teichoic acid Length = 432 Score = 89.4 bits (220), Expect = 1e-17, Method: Compositional matrix adjust. Identities = 25/406 (6%), Positives = 72/406 (17%), Gaps = 21/406 (5%) Query: 19 FIVKLTGLIGRVILTRIVGAEGIGLYQIAYSFYGFVLMFTG-GLPTTLAMASAKKPA-QS 76 I + V + ++ + Sbjct: 18 TIAADSIETAVKSELVNVAKKVRIDGFRKGKVPMNIVAQRYGASVRQDVLGDLMSRNFID 77 Query: 77 WSLLK---IISLGVILFGGIV-SMAVFWNSPAI-SRFLGNPGLEYSIRGLAPSXXXLFAV 131 + + + G ++ + GLE Sbjct: 78 AIIKEKINPAGAPTYVPGEYKLGEDFTYSVEFEVYPEVELQGLEAIEVEKPI------VE 131 Query: 132 PLLGL---VRGYLQGHKQIGVIALSEIIEQASRIFFMLLIVWHVLPLGINRAIGSGLYGT 188 + L+ + + + V G + G Sbjct: 132 VTDADVDGMLDTLRKQQATWKEKDGAVEAEDRVTIDFTGSVDGEEFEGGKASDFVLAMGQ 191 Query: 189 FIGALSAFSLLTIYISVNKTSLPYNT---SYTVQSLPIFWFIQS-SLMISATRLIIPASE 244 + T Y ++L + +L R + + Sbjct: 192 GRMIPGFE-DGIKGHKAGEEFTIDVTFPEEYHAENLKGKAAKFAINLKKVEERELPELTA 250 Query: 245 FIDAVLIPNRLLAAGYSTSEATSMYGVIYGMAVIVAYAPTLLTGALCHTLSVQIAAEWQQ 304 G +M + + + + + V A + Sbjct: 251 EFIKRFGVEDGSVEGLRAEVRKNMERELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSE 310 Query: 305 GNMQRFNRLSATAFKVCWLWGIATALFLKGYAPELSLFIFNTETAGPAIKYLAAIPLLVG 364 ++ R + LF + + + + E + Sbjct: 311 IDVLRRQAAQRFGGNEKQALELPRELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGL 370 Query: 365 FREISTSILWSQDIRKSPFYGLLTGIICATAAQYFLVGIPGFGYKG 410 E++++ +++ + A Sbjct: 371 IEEMASAYEDPKEVIEFYSKNKELMDNMRNVALEEQAVEAVLAKAK 416 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.327 0.141 0.427 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 31,106,625 Number of extensions: 1212317 Number of successful extensions: 4985 Number of sequences better than 1.0e-05: 19 Number of HSP's gapped: 4963 Number of HSP's successfully gapped: 19 Length of query: 499 Length of database: 38,378,431 Length adjustment: 111 Effective length of query: 388 Effective length of database: 25,576,357 Effective search space: 9923626516 Effective search space used: 9923626516 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 118 (50.1 bits)