BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0580.0023.p (605 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH0941 [V] COG1132 ABC-type multidrug transport system, ATPase a... 360 5e-99 BS_ygaD [V] COG1132 ABC-type multidrug transport system, ATPase ... 354 3e-97 YPO1735 [V] COG1132 ABC-type multidrug transport system, ATPase ... 345 1e-94 CAC1613 [V] COG1132 ABC-type multidrug transport system, ATPase ... 337 4e-92 lin0155 [V] COG1132 ABC-type multidrug transport system, ATPase ... 328 2e-89 >BH0941 [V] COG1132 ABC-type multidrug transport system, ATPase and permease components Length = 584 Score = 360 bits (923), Expect = 5e-99, Method: Compositional matrix adjust. Identities = 202/568 (35%), Positives = 317/568 (55%), Gaps = 10/568 (1%) Query: 39 PYWKQTLLVMAVLIVSAVLGLLPPLFIQQIIDQALPDKNFRXXXXXXXXXXXXXXXXGLL 98 PYWKQ + + + ++ + LL PL + IID + + + Sbjct: 16 PYWKQIFITIIIGLLKFGIPLLIPLLVAYIIDGIIFAEQMTNEEKFSALFTVMGGIFFIF 75 Query: 99 GVLQ-------NYLNSFISLHIVYDMKNQMYRHLQKMQLQFYSGVQQGEVITRMTSDISG 151 VL+ Y +I I+YD+++ ++ H+QK+ L+FYS + GE+I+R+ D+ Sbjct: 76 IVLRPPIEYYRQYFAQWIGNKILYDIRDHLFTHIQKLSLRFYSNRKVGEIISRVIHDVEQ 135 Query: 152 VQGVFSSTVVNFASNLFILVSTAVTLFIMNWKLALLGIFVIPLFVVPTRKMGNLSWKLAK 211 + + ++N ++F ++ +F MN L L+ I + P + + L + Sbjct: 136 TKTFVMTGLMNIWLDMFTIIIALAIMFSMNAWLTLVAIAMFPFYGFSIKYFYAKLRHLTR 195 Query: 212 QTQEKISEQNQIIQETLSISGYLLMKLFTKESAEYTKFQTVNSEATSLQIRQSMAGRWFM 271 + +++ + E + G +++ F E E T+F N + I + Sbjct: 196 VRSQALADVQGHLHE--RVQGMSVIRSFALEDYEQTQFAKRNGTFLNRAIDHTKWNAKTF 253 Query: 272 MVLSTFTSXXXXXXXXXXXXXXXHGELSIGAIVAFVALLGRLYGPVMQMTNLYVEIKRSA 331 V++T T +GEL +GA+ AFV + RLY P+ ++ N + +S Sbjct: 254 AVVNTVTDIAPLLVIFVAACFVINGELEVGAMTAFVLYMERLYNPLRRLVNSSTTLTQSI 313 Query: 332 ALFERIFDYFDMKPLIVDKPEARFISAAGRDITFHEVSFAYQADKT-ALHGISFXXXXXX 390 A +R+F++ D K IVDK A + D+TF VSF+Y+ ++T AL + F Sbjct: 314 ASMDRVFEFIDEKYDIVDKEGAVKLEEVHGDVTFDHVSFSYEKEETPALKDVHFHVKRGE 373 Query: 391 XXXXVGPSGAGKTTITNLIPRLYETDSGAITIGGSNIRDFTLESLRAQIGLVTQDTYLFN 450 VG SG GK+T+ +LIPR Y+ SG I I G +IRD+ + SLR QIG+V Q+ LF+ Sbjct: 374 TVAFVGMSGGGKSTLISLIPRFYDVSSGRILIDGHDIRDYQVRSLRDQIGMVLQENILFS 433 Query: 451 GTIRENLIYADPGASEDRIIAVCQAAYIHDFIMSLPEGYDTVVGNRGVKLSGGEKQRISI 510 ++R N++ P A+E+ +IA +AA HDFIM+LP GYDT VG RGVKLSGG+KQR++I Sbjct: 434 DSVRMNILMGKPDATEEEVIAAAKAANAHDFIMNLPNGYDTEVGERGVKLSGGQKQRVAI 493 Query: 511 ARVLLKNPPVIIMDEATSSLDTASEYYIQQAMHTLLQGKTSIVIAHRLSTIMAADQILVV 570 ARV LKNPP++I DEATS+LD SE+YIQ+AM L + +T+ ++AHRLSTI AD+I+VV Sbjct: 494 ARVFLKNPPILIFDEATSALDLESEHYIQEAMEKLAKNRTTFIVAHRLSTITHADRIIVV 553 Query: 571 QAGTIVEAGTHQSLLEQHGVYRDLYDKQ 598 + G IVE+GTH L+ +GVY+ L++ Q Sbjct: 554 ENGEIVESGTHDELMSCNGVYKKLFEVQ 581 >BS_ygaD [V] COG1132 ABC-type multidrug transport system, ATPase and permease components Length = 165 Score = 354 bits (908), Expect = 3e-97, Method: Compositional matrix adjust. Identities = 0/578 (0%), Positives = 0/578 (0%), Gaps = 9/578 (1%) Query: 28 ALLLRIAGYFFPYWKQTLLVMAVLIVSAVLGLLPPLFIQQIIDQAL------PDKNFRXX 81 + + + + + + R Sbjct: 11 EQVGQALKARGATVTTAESCTGGWVAKVITDIAGSSAWFERGFVTYSNEAKAQMIGVREE 70 Query: 82 XXXXXXXXXXXXXXGLLGVLQNYLNSFISLHIVYDMKNQMYRHLQKMQLQFYSGVQQGEV 141 + + ++ I + + +++ Sbjct: 71 TLAQHGAVSEPVVVEMAIGALKAARADYAVSISGIAGPDGGSEEKPVGTVWFAFATARGE 130 Query: 142 ITRMTSDISGVQGVFSSTVVNFASNLFILVSTAVTLFIMNWKLALLGIFVIPLFVVPTRK 201 Sbjct: 131 190 Query: 202 MGNLSWKLAKQTQEKISEQNQIIQETLSISGYLLMKLFTKESAEYTKFQTVNSEATSLQI 261 Sbjct: 191 -- 248 Query: 262 RQSMAGRWFMMVLSTFTSXXXXXXXXXXXXXXXHGELSIGAIVAFVALLGRLYGPVMQMT 321 Sbjct: 249 308 Query: 322 NLYVEIKRSAALFERIFDYFDMKPLIVDKPEARFISAAGRDITFHEVSFAYQADK-TALH 380 Sbjct: 309 368 Query: 381 GISFXXXXXXXXXXVGPSGAGKTTITNLIPRLYETDSGAITIGGSNIRDFTLESLRAQIG 440 Sbjct: 369 428 Query: 441 LVTQDTYLFNGTIRENLIYADPGASEDRIIAVCQAAYIHDFIMSLPEGYDTVVGNRGVKL 500 Sbjct: 429 488 Query: 501 SGGEKQRISIARVLLKNPPVIIMDEATSSLDTASEYYIQQAMHTLLQGKTSIVIAHRLST 560 Sbjct: 489 548 Query: 561 IMAADQILVVQAGTIVEAGTHQSLLEQHGVYRDLYDKQ 598 Sbjct: 549 586 >YPO1735 [V] COG1132 ABC-type multidrug transport system, ATPase and permease components Length = 572 Score = 345 bits (884), Expect = 1e-94, Method: Compositional matrix adjust. Identities = 208/577 (36%), Positives = 306/577 (53%), Gaps = 14/577 (2%) Query: 29 LLLRIAGYFFPYWKQTLLVMAVLIVSAVLGLLPPLFIQQIIDQALPDKNFRXXXXXXXXX 88 +L R Y+ PY L I++ +L L P+ ++ ID+ LP++++ Sbjct: 1 MLRRFFSYYAPYKGLFFLDFGCAILAGLLELSFPMAVKLFIDKLLPNQDWVMIVWAAAGL 60 Query: 89 XXXXXXXGLLGVLQNYLNSFISLHIVYDMKNQMYRHLQKMQLQFYSGVQQGEVITRMTSD 148 +L + NY + + I DM+ Q + HLQK+ +Y ++ G +IT +T D Sbjct: 61 LLIYLLNTVLMAIVNYWGHALGVGIETDMRRQAFNHLQKLSFSYYDNIKTGHIITHVTKD 120 Query: 149 ISGVQGVFSSTVVNFASNLFILVSTAVTLFIM----NWKLALLGIFVIPLFVVPTRKMGN 204 + V V +LFI V T + FI+ + LA++ I ++P + G+ Sbjct: 121 LEEVGEVAHHG----PEDLFIAVMTFIGAFILMATVHLPLAMVTIIIVPFMTYLVTRYGS 176 Query: 205 LSWKLAKQTQEKISEQNQIIQETLSISGYLLMKLFTKESAEYTKF--QTVNSEATSLQIR 262 ++ ++ N I+E SI G ++K F E E F N T L+ Sbjct: 177 KMTDTWRKLFGQVGNFNTRIEE--SIGGIRVVKAFANEPHEEKLFAKDNENYRTTKLRAY 234 Query: 263 QSMAGRWFMMVLSTFTSXXXXXXXXXXXXXXXHGELSIGAIVAFVALLGRLYGPVMQMTN 322 + M M LST H +LS G V F+ L+ + PV ++T+ Sbjct: 235 RIMTTSMTMSYLST--RLVQLIVMVVGTWYVIHDQLSYGGFVGFLLLVEVFFRPVEKITS 292 Query: 323 LYVEIKRSAALFERIFDYFDMKPLIVDKPEARFISAAGRDITFHEVSFAYQADKTALHGI 382 + + A F+R D P IVD+P AR + DI++ V F Y + + Sbjct: 293 VLESYPKGIAGFKRFTQLIDTLPDIVDQPNARPVGHLRGDISYQHVCFGYSSQAPIFTDL 352 Query: 383 SFXXXXXXXXXXVGPSGAGKTTITNLIPRLYETDSGAITIGGSNIRDFTLESLRAQIGLV 442 + VGPSGAGKTT+ +L+PR YE ++GAITI G +IR+ T +SLR IG+V Sbjct: 353 NLQIHAGETVAFVGPSGAGKTTLCSLLPRFYELNAGAITIDGIDIREMTQQSLRNNIGIV 412 Query: 443 TQDTYLFNGTIRENLIYADPGASEDRIIAVCQAAYIHDFIMSLPEGYDTVVGNRGVKLSG 502 QD +LF G+IREN+ Y AS+D I+ Q A + + I SLPEG DT+VG RGVKLSG Sbjct: 413 QQDVFLFGGSIRENIAYGKLNASDDEIMVAAQRARLDELISSLPEGLDTLVGERGVKLSG 472 Query: 503 GEKQRISIARVLLKNPPVIIMDEATSSLDTASEYYIQQAMHTLLQGKTSIVIAHRLSTIM 562 G+KQR+SIAR+ LKNPP++I+DEATS+LDTA+E IQ A+ L QG+T++VIAHRL+TI Sbjct: 473 GQKQRLSIARIFLKNPPILILDEATSALDTATEQAIQLALTELSQGRTTLVIAHRLATIQ 532 Query: 563 AADQILVVQAGTIVEAGTHQSLLEQHGVYRDLYDKQY 599 AD+I+VV G I+E G H LL + G Y L+ Q+ Sbjct: 533 NADRIIVVDKGGIIEQGDHHELLARQGAYAILHHAQF 569 >CAC1613 [V] COG1132 ABC-type multidrug transport system, ATPase and permease components Length = 579 Score = 337 bits (863), Expect = 4e-92, Method: Compositional matrix adjust. Identities = 187/486 (38%), Positives = 273/486 (56%), Gaps = 4/486 (0%) Query: 116 DMKNQMYRHLQKMQLQFYSGVQQGEVITRMTSDISGVQGVFSSTVVN-FASNLFILVSTA 174 DM+ ++ HLQ + +Y GE+++++ SD+ + + N F S L IL + Sbjct: 93 DMRQDLFYHLQMLPFSYYDENNTGEMMSKLVSDLFDISELAHHGPENVFISALKIL-GSF 151 Query: 175 VTLFIMNWKLALLGIFVIPLFVVPTRKMGNLSWKLAKQTQEKISEQNQIIQETLSISGYL 234 V L ++N K+ + V + V+ + K+ ++KI+ N +Q++L G Sbjct: 152 VILMMINVKMTFILFVVTLIMVIFSINRNTKMQKIFFDNRKKIANVNSSVQDSLE--GIR 209 Query: 235 LMKLFTKESAEYTKFQTVNSEATSLQIRQSMAGRWFMMVLSTFTSXXXXXXXXXXXXXXX 294 ++K F ES E KF N+ +++ A F+ S F Sbjct: 210 VVKSFANESVEQKKFDVSNNNYLKSKVKSYKAMGEFIAGSSLFQGMLYLSIIVSGAVFIK 269 Query: 295 HGELSIGAIVAFVALLGRLYGPVMQMTNLYVEIKRSAALFERIFDYFDMKPLIVDKPEAR 354 G L I + + + P+ + N + ++ A F+R + + KP I+DKP A Sbjct: 270 DGTLKISDLAIYALYINIFINPIDVLINFMEQFQKGYAGFKRFIEVVETKPSILDKPGAE 329 Query: 355 FISAAGRDITFHEVSFAYQADKTALHGISFXXXXXXXXXXVGPSGAGKTTITNLIPRLYE 414 I +I F +V F Y +H ++ VGPSG GKTTI +L+PR Y+ Sbjct: 330 EIKNVKGNIKFKDVCFKYDDAHEVIHNLNINIDAGKTVALVGPSGGGKTTICSLLPRFYD 389 Query: 415 TDSGAITIGGSNIRDFTLESLRAQIGLVTQDTYLFNGTIRENLIYADPGASEDRIIAVCQ 474 SG+ITI ++IRD TL SLR IG+V QD Y+F+GTIR+N+ Y P ASE+ +I + Sbjct: 390 VTSGSITIDDNDIRDVTLNSLRNAIGVVQQDVYIFSGTIRDNIAYGRPEASEEEVIEAAK 449 Query: 475 AAYIHDFIMSLPEGYDTVVGNRGVKLSGGEKQRISIARVLLKNPPVIIMDEATSSLDTAS 534 A IHD+IMSL +GYDT VG RGVKLSGG+KQRISIARV LKNPP++I+DEATS+LD S Sbjct: 450 GANIHDYIMSLEDGYDTFVGERGVKLSGGQKQRISIARVFLKNPPILILDEATSALDNES 509 Query: 535 EYYIQQAMHTLLQGKTSIVIAHRLSTIMAADQILVVQAGTIVEAGTHQSLLEQHGVYRDL 594 E YIQ+++ L G+TSIVIAHRLSTI AD+I+V++ I E G H+ LL++ GVY Sbjct: 510 EKYIQKSLEKLSSGRTSIVIAHRLSTIRNADEIIVIKDRGIKEKGNHEELLKKGGVYSYY 569 Query: 595 YDKQYE 600 Y+ Q+E Sbjct: 570 YNMQFE 575 >lin0155 [V] COG1132 ABC-type multidrug transport system, ATPase and permease components Length = 593 Score = 328 bits (841), Expect = 2e-89, Method: Compositional matrix adjust. Identities = 192/593 (32%), Positives = 312/593 (52%), Gaps = 10/593 (1%) Query: 12 GRLKFD-DDDKKTNLSKALLLRIAGYFFPYWKQTLLVMAVLIVSAVLGLLPPLFIQQIID 70 R KFD D++ +T S A L RI Y PY K + + V++++ V ++ P + +ID Sbjct: 2 ARNKFDIDEELETAFSAAHLKRILVYVKPYQKSIYITLFVILLANVATMIGPYLTKVVID 61 Query: 71 QALPDKNFRXXXXXXXXXXXXXXXXGLLGVLQNYLNSFISLHIVYDMKNQMYRHLQKMQL 130 + +P+KN GL + + I I+ DM+ ++ HLQK+ Sbjct: 62 ETIPNKNMTQLFWIAVIFIVSVIVTGLCMRYRIRSITLIGQDILKDMRTAIFGHLQKLPF 121 Query: 131 QFYSGVQQGEVITRMTSDISGVQGVFSSTVVNFASNLFILVSTAVTLFIMNWKLALLGIF 190 ++ G+++ R+ + I+ + + S+ ++N S++ ++ T + +++ L L + Sbjct: 122 SYFDSRPHGKILIRVVNYINMLSDLLSNGLINLISDILSVIVTLGFMLMIDPVLTLYSLA 181 Query: 191 VIPLFVVPTRKMGNLSWKLAKQTQEKISEQNQIIQETLSISGYLLMKLFTKESAEYTKFQ 250 +IP+ V + K + K S N I E SI+G + + F++E + F Sbjct: 182 LIPVLFVIVMVIKTAQRKAYQVLSNKQSNMNAYIHE--SIAGIKVTQSFSREEENFEIFT 239 Query: 251 TVNSEATSLQIRQSMAGRWFMMVLSTFTSXXXXXXXXXXXXXXXHG---ELSIGAIVAFV 307 V++E R M +L G ++S G ++AF+ Sbjct: 240 EVSNEYR----RSWMKAVKIQFLLWPGVQNIAVMTTCLIYFVGIKGYGVDVSTGTLIAFI 295 Query: 308 ALLGRLYGPVMQMTNLYVEIKRSAALFERIFDYFDMKPLIVDKPEARFISAAGRDITFHE 367 +G + PV+ + N Y + + ERIF+ D++P I D P A+ + ++ F + Sbjct: 296 GYVGNFWNPVINIGNFYNSLITATTYLERIFETMDVEPDIKDVPNAKKMPPIVGNVDFKD 355 Query: 368 VSFAYQADKTALHGISFXXXXXXXXXXVGPSGAGKTTITNLIPRLYETDSGAITIGGSNI 427 V F Y+ L GI+F VGP+GAGKTTI NL+ R Y +SG I + G N+ Sbjct: 356 VYFRYEEGVDILKGINFHVDAGESIALVGPTGAGKTTIINLLSRFYNINSGEILVDGENV 415 Query: 428 RDFTLESLRAQIGLVTQDTYLFNGTIRENLIYADPGASEDRIIAVCQAAYIHDFIMSLPE 487 + TL SLR+Q+G++ QDT++F+GTI EN+ Y A+E+ IIA + HDFI L + Sbjct: 416 EEVTLRSLRSQMGVMLQDTFIFSGTIIENIRYGKLDATEEEIIAAAKVVRAHDFISGLKD 475 Query: 488 GYDTVVGNRGVKLSGGEKQRISIARVLLKNPPVIIMDEATSSLDTASEYYIQQAMHTLLQ 547 GY T V RG LS G++Q IS AR LL +P ++I+DEATSS+DT +E +Q+ + LL+ Sbjct: 476 GYYTEVKERGSTLSAGQRQLISFARALLADPKILILDEATSSIDTQTEILLQEGLERLLE 535 Query: 548 GKTSIVIAHRLSTIMAADQILVVQAGTIVEAGTHQSLLEQHGVYRDLYDKQYE 600 G+TS +IAHRLSTI + +I + G I EAG+H+ L+ QHG Y +LY Q++ Sbjct: 536 GRTSFIIAHRLSTIKNSSRIFYIDNGRIQEAGSHEELMAQHGYYYNLYQSQFD 588 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.322 0.137 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 37,703,860 Number of extensions: 1400127 Number of successful extensions: 9313 Number of sequences better than 1.0e-05: 1782 Number of HSP's gapped: 7407 Number of HSP's successfully gapped: 1884 Length of query: 605 Length of database: 38,378,431 Length adjustment: 112 Effective length of query: 493 Effective length of database: 25,461,023 Effective search space: 12552284339 Effective search space used: 12552284339 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 119 (50.4 bits)